ID   RTA1_YEAST              Reviewed;         317 AA.
AC   P53047; D6VUZ6; E9P922;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   24-JAN-2024, entry version 142.
DE   RecName: Full=Protein RTA1;
GN   Name=RTA1; OrderedLocusNames=YGR213C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RX   PubMed=8660468; DOI=10.1007/s002940050110;
RA   Soustre I., Letourneux Y., Karst F.;
RT   "Characterization of the Saccharomyces cerevisiae RTA1 gene involved in 7-
RT   aminocholesterol resistance.";
RL   Curr. Genet. 30:121-125(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9290212;
RX   DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA   Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT   "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT   chromosome VII.";
RL   Yeast 13:1077-1090(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- FUNCTION: Involved in 7-aminocholesterol resistance.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the lipid-translocating exporter (LTE)
CC       (TC 9.A.26.1) family. {ECO:0000305}.
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DR   EMBL; X84736; CAA59227.1; -; Genomic_DNA.
DR   EMBL; Z72998; CAA97240.1; -; Genomic_DNA.
DR   EMBL; AY693143; AAT93162.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08307.1; -; Genomic_DNA.
DR   PIR; S64536; S64536.
DR   RefSeq; NP_011729.1; NM_001181342.1.
DR   AlphaFoldDB; P53047; -.
DR   SMR; P53047; -.
DR   BioGRID; 33466; 19.
DR   DIP; DIP-1599N; -.
DR   IntAct; P53047; 1.
DR   MINT; P53047; -.
DR   STRING; 4932.YGR213C; -.
DR   TCDB; 9.A.26.1.1; the lipid-translocating exporter (lte) family.
DR   PaxDb; 4932-YGR213C; -.
DR   PeptideAtlas; P53047; -.
DR   EnsemblFungi; YGR213C_mRNA; YGR213C; YGR213C.
DR   GeneID; 853127; -.
DR   KEGG; sce:YGR213C; -.
DR   AGR; SGD:S000003445; -.
DR   SGD; S000003445; RTA1.
DR   VEuPathDB; FungiDB:YGR213C; -.
DR   eggNOG; ENOG502QURG; Eukaryota.
DR   GeneTree; ENSGT00940000176387; -.
DR   HOGENOM; CLU_033465_3_1_1; -.
DR   InParanoid; P53047; -.
DR   OMA; FRCKSSK; -.
DR   OrthoDB; 10482at2759; -.
DR   BioCyc; YEAST:G3O-30895-MONOMER; -.
DR   BioGRID-ORCS; 853127; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P53047; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P53047; Protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   InterPro; IPR007568; RTA1.
DR   PANTHER; PTHR31465; PROTEIN RTA1-RELATED; 1.
DR   PANTHER; PTHR31465:SF1; PROTEIN RTA1-RELATED; 1.
DR   Pfam; PF04479; RTA1; 1.
PE   1: Evidence at protein level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..317
FT                   /note="Protein RTA1"
FT                   /id="PRO_0000097510"
FT   TOPO_DOM        1..16
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        38..64
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        86..99
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        121..142
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        164..178
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        179..199
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        200..214
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        215..235
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        236..259
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        281..317
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        234
FT                   /note="I -> V (in Ref. 5; AAT93162)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   317 AA;  35486 MW;  D4CF78080D129B4A CRC64;
     MAKDGFELYR YTPELGASIL FTVLFAVSGV AFVILLFHYS VKSKRRVGSL MKSQPVLRYY
     GTVNLAGAYI PFIFGCFVEC VGFAFRCKSS KDTTLLNPYI IQTVFLLVSP TLYAASIYMI
     FGRMATLLFA ENLMIMPARF NTTIFVIGDV GSLLLQAIGG AMMSKVTSAS SGSHLVTAGL
     FIQIAFFGLF IINEVLFIFK MSKKPTNVSV RYGSWKYLNI ALLVNSFLIL IRSIVRAVEF
     IQGYDGEIAS HEWYLYIFDG LPMFLLVLIF IVAFPLINIF RIHEESIQAQ QSARFDGTDY
     PDVEVTSIEE DLASKSE
//