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Protein

Methylsterol monooxygenase

Gene

ERG25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol.2 Publications

Miscellaneous

Present with 77100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O.1 Publication

Cofactori

Fe cation1 Publication

Pathwayi: zymosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (ERG11)
  2. Delta(14)-sterol reductase (ERG24)
  3. Methylsterol monooxygenase (ERG25)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (ERG26)
  5. 3-keto-steroid reductase (ERG27)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

  • C-4 methylsterol oxidase activity Source: SGD
  • iron ion binding Source: InterPro

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandIron, NAD

Enzyme and pathway databases

BioCyciMetaCyc:YGR060W-MONOMER
YEAST:YGR060W-MONOMER
ReactomeiR-SCE-191273 Cholesterol biosynthesis
R-SCE-192105 Synthesis of bile acids and bile salts
UniPathwayiUPA00770; UER00756

Names & Taxonomyi

Protein namesi
Recommended name:
Methylsterol monooxygenase (EC:1.14.18.91 Publication)
Alternative name(s):
C-4 methylsterol oxidase
Gene namesi
Name:ERG25
Synonyms:FET6
Ordered Locus Names:YGR060W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR060W
SGDiS000003292 ERG25

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei56 – 76HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170391 – 309Methylsterol monooxygenaseAdd BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki96Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP53045
PaxDbiP53045
PRIDEiP53045

PTM databases

iPTMnetiP53045

Interactioni

Subunit structurei

Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Interacts with ERG27 and ERG28.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33305, 311 interactors
DIPiDIP-2799N
IntActiP53045, 60 interactors
MINTiP53045
STRINGi4932.YGR060W

Structurei

3D structure databases

ProteinModelPortaliP53045
SMRiP53045
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi160 – 164Histidine box-15
Motifi173 – 177Histidine box-25
Motifi257 – 263Histidine box-37

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063017
HOGENOMiHOG000162289
InParanoidiP53045
KOiK07750
OMAiQFNCNFA
OrthoDBiEOG092C3Y3T

Family and domain databases

InterProiView protein in InterPro
IPR006694 Fatty_acid_hydroxylase
PfamiView protein in Pfam
PF04116 FA_hydroxylase, 1 hit

Sequencei

Sequence statusi: Complete.

P53045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVFNNATL SGLVQASTYS QTLQNVAHYQ PQLNFMEKYW AAWYSYMNND
60 70 80 90 100
VLATGLMFFL LHEFMYFFRC LPWFIIDQIP YFRRWKLQPT KIPSAKEQLY
110 120 130 140 150
CLKSVLLSHF LVEAIPIWTF HPMCEKLGIT VEVPFPSLKT MALEIGLFFV
160 170 180 190 200
LEDTWHYWAH RLFHYGVFYK YIHKQHHRYA APFGLSAEYA HPAETLSLGF
210 220 230 240 250
GTVGMPILYV MYTGKLHLFT LCVWITLRLF QAVDSHSGYD FPWSLNKIMP
260 270 280 290 300
FWAGAEHHDL HHHYFIGNYA SSFRWWDYCL DTESGPEAKA SREERMKKRA

ENNAQKKTN
Length:309
Mass (Da):36,479
Last modified:October 1, 1996 - v1
Checksum:iE0422C416DD17794
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302N → K in AAS56153 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31885 Genomic DNA Translation: AAC49139.1
DQ115391 Genomic DNA Translation: AAZ22473.1
Z72845 Genomic DNA Translation: CAA97062.1
AY557827 Genomic DNA Translation: AAS56153.1
BK006941 Genomic DNA Translation: DAA08156.1
PIRiS64354
RefSeqiNP_011574.3, NM_001181189.3

Genome annotation databases

EnsemblFungiiYGR060W; YGR060W; YGR060W
GeneIDi852951
KEGGisce:YGR060W

Similar proteinsi

Entry informationi

Entry nameiMSMO_YEAST
AccessioniPrimary (citable) accession number: P53045
Secondary accession number(s): D6VUJ5, E9P8T3, Q45U26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 20, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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