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Protein

Ubiquitin fusion degradation protein 1

Gene

UFD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions at a post-ubiquitation step in the ubiquitin fusion degradation (UFD) pathway. Has a role in the endoplasmic reticulum-associated degradation (ERAD) pathway. Required for the proteasome-dependent processing/activation of MGA2 and SPT23 transcription factors leading to the subsequent expression of OLE1. Has an additional role in the turnover of OLE1 where it targets ubiquitinated OLE1 and other proteins to the ERAD.4 Publications

Miscellaneous

Present with 3530 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei37Monoubiquitin-binding1
Sitei39Monoubiquitin-binding1
Sitei109Monoubiquitin-binding1

GO - Molecular functioni

  • polyubiquitin modification-dependent protein binding Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

  • cytoplasm protein quality control by the ubiquitin-proteasome system Source: SGD
  • ER-associated misfolded protein catabolic process Source: SGD
  • negative regulation of telomerase activity Source: SGD
  • nonfunctional rRNA decay Source: SGD
  • positive regulation of protein localization to nucleus Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • retrograde protein transport, ER to cytosol Source: SGD
  • ribosome-associated ubiquitin-dependent protein catabolic process Source: SGD
  • ubiquitin-dependent ERAD pathway Source: SGD

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30766-MONOMER
ReactomeiR-SCE-110320 Translesion Synthesis by POLH

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin fusion degradation protein 1
Short name:
UB fusion protein 1
Alternative name(s):
Polymerase-interacting protein 3
Gene namesi
Name:UFD1
Synonyms:PIP3
Ordered Locus Names:YGR048W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR048W
SGDiS000003280 UFD1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94V → D in UFD1-1; grows more slowly. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949891 – 361Ubiquitin fusion degradation protein 1Add BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53044
PaxDbiP53044
PRIDEiP53044

PTM databases

CarbonylDBiP53044
iPTMnetiP53044

Interactioni

Subunit structurei

Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factor YOS9, and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1. Interacts with NPL4, CDC48 AND UBX2.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • polyubiquitin modification-dependent protein binding Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi33295, 171 interactors
DIPiDIP-1476N
IntActiP53044, 20 interactors
MINTiP53044
STRINGi4932.YGR048W

Structurei

Secondary structure

1361
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 29Combined sources11
Helixi30 – 32Combined sources3
Turni35 – 37Combined sources3
Helixi40 – 44Combined sources5
Beta strandi45 – 50Combined sources6
Helixi52 – 60Combined sources9
Beta strandi69 – 72Combined sources4
Turni74 – 76Combined sources3
Beta strandi79 – 87Combined sources9
Beta strandi93 – 96Combined sources4
Helixi98 – 104Combined sources7
Beta strandi111 – 118Combined sources8
Beta strandi123 – 128Combined sources6
Helixi131 – 135Combined sources5
Helixi140 – 150Combined sources11
Beta strandi154 – 164Combined sources11
Beta strandi167 – 177Combined sources11
Beta strandi188 – 190Combined sources3
Beta strandi192 – 196Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZC1NMR-A1-208[»]
ProteinModelPortaliP53044
SMRiP53044
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53044

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 28Monoubiquitin-binding2
Regioni30 – 32Monoubiquitin-binding3
Regioni99 – 101Monoubiquitin-binding3

Sequence similaritiesi

Belongs to the UFD1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002408
HOGENOMiHOG000212737
InParanoidiP53044
KOiK14016
OMAiFFGYEIK
OrthoDBiEOG092C4VXI

Family and domain databases

InterProiView protein in InterPro
IPR004854 Ufd1-like
PANTHERiPTHR12555 PTHR12555, 1 hit
PfamiView protein in Pfam
PF03152 UFD1, 1 hit

Sequencei

Sequence statusi: Complete.

P53044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSGFSSFGG GNGFVNMPQT FEEFFRCYPI AMMNDRIRKD DANFGGKIFL
60 70 80 90 100
PPSALSKLSM LNIRYPMLFK LTANETGRVT HGGVLEFIAE EGRVYLPQWM
110 120 130 140 150
METLGIQPGS LLQISSTDVP LGQFVKLEPQ SVDFLDISDP KAVLENVLRN
160 170 180 190 200
FSTLTVDDVI EISYNGKTFK IKILEVKPES SSKSICVIET DLVTDFAPPV
210 220 230 240 250
GYVEPDYKAL KAQQDKEKKN SFGKGQVLDP SVLGQGSMST RIDYAGIANS
260 270 280 290 300
SRNKLSKFVG QGQNISGKAP KAEPKQDIKD MKITFDGEPA KLDLPEGQLF
310 320 330 340 350
FGFPMVLPKE DEESAAGSKS SEQNFQGQGI SLRKSNKRKT KSDHDSSKSK
360
APKSPEVIEI D
Length:361
Mass (Da):39,810
Last modified:October 1, 1996 - v1
Checksum:i198E0CD48B863B98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22153 Genomic DNA Translation: AAC49023.1
U17264 Genomic DNA Translation: AAB46627.1
DQ115391 Genomic DNA Translation: AAZ22463.1
Z72833 Genomic DNA Translation: CAA97047.1
AY692806 Genomic DNA Translation: AAT92825.1
BK006941 Genomic DNA Translation: DAA08146.1
PIRiS59814
RefSeqiNP_011562.1, NM_001181177.1

Genome annotation databases

EnsemblFungiiYGR048W; YGR048W; YGR048W
GeneIDi852939
KEGGisce:YGR048W

Similar proteinsi

Entry informationi

Entry nameiUFD1_YEAST
AccessioniPrimary (citable) accession number: P53044
Secondary accession number(s): D6VUI5, Q45U36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health