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Protein

Serine/threonine-protein phosphatase T

Gene

PPT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90 (HSC82 and HSP82). Dephosphorylation positively regulates the Hsp90 chaperone machinery.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Stimulated by arachidonic acid and other unsaturated fatty acids, and by arachidoyl coenzyme A.1 Publication

Kineticsi

  1. KM=34 mM for p-nitrophenylphosphate1 Publication

    pH dependencei

    Optimum pH is 7.8.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi249Manganese 1By similarity1
    Metal bindingi251Manganese 1By similarity1
    Metal bindingi278Manganese 1By similarity1
    Metal bindingi278Manganese 2By similarity1
    Metal bindingi310Manganese 2By similarity1
    Active sitei311Proton donorBy similarity1
    Metal bindingi359Manganese 2By similarity1
    Metal bindingi434Manganese 2By similarity1

    GO - Molecular functioni

    • metal ion binding Source: UniProtKB-KW
    • protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    • protein dephosphorylation Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30830-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase T (EC:3.1.3.16)
    Short name:
    PPT
    Gene namesi
    Name:PPT1
    Ordered Locus Names:YGR123C
    ORF Names:G6347
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VII

    Organism-specific databases

    EuPathDBiFungiDB:YGR123C.
    SGDiS000003355. PPT1.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: SGD
    • nucleus Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi311H → A: Loss of phosphatase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000588981 – 513Serine/threonine-protein phosphatase TAdd BLAST513

    Proteomic databases

    MaxQBiP53043.
    PRIDEiP53043.

    Expressioni

    Inductioni

    Expression peaks in early log phase and decreases dramatically during the stationary phase (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Interacts (via TPR repeats) with HSP82 (via C-terminal MEEVD pentapeptide).1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HSC82P151083EBI-13796,EBI-8666
    HSP82P028298EBI-13796,EBI-8659

    Protein-protein interaction databases

    BioGridi33370. 30 interactors.
    DIPiDIP-1525N.
    IntActiP53043. 10 interactors.
    MINTiMINT-406578.

    Structurei

    Secondary structure

    1513
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi192 – 201Combined sources10
    Helixi203 – 205Combined sources3
    Helixi211 – 226Combined sources16
    Beta strandi230 – 234Combined sources5
    Beta strandi237 – 239Combined sources3
    Beta strandi243 – 247Combined sources5
    Helixi254 – 264Combined sources11
    Beta strandi271 – 275Combined sources5
    Beta strandi280 – 283Combined sources4
    Helixi286 – 299Combined sources14
    Turni301 – 303Combined sources3
    Beta strandi304 – 306Combined sources3
    Helixi314 – 320Combined sources7
    Helixi322 – 329Combined sources8
    Helixi332 – 342Combined sources11
    Beta strandi347 – 351Combined sources5
    Turni352 – 354Combined sources3
    Beta strandi355 – 357Combined sources3
    Helixi370 – 374Combined sources5
    Beta strandi384 – 386Combined sources3
    Helixi387 – 393Combined sources7
    Beta strandi398 – 404Combined sources7
    Beta strandi411 – 413Combined sources3
    Helixi415 – 424Combined sources10
    Beta strandi428 – 432Combined sources5
    Beta strandi439 – 444Combined sources6
    Helixi445 – 447Combined sources3
    Beta strandi449 – 452Combined sources4
    Helixi458 – 460Combined sources3
    Beta strandi465 – 471Combined sources7
    Turni480 – 482Combined sources3
    Beta strandi488 – 493Combined sources6
    Turni503 – 506Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3ICFX-ray2.30A/B179-513[»]
    ProteinModelPortaliP53043.
    SMRiP53043.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP53043.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Repeati12 – 45TPR 1Add BLAST34
    Repeati46 – 79TPR 2Add BLAST34
    Repeati80 – 113TPR 3Add BLAST34

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni188 – 513CatalyticAdd BLAST326

    Domaini

    The TPR repeats mediate protein-protein interactions with substrate proteins, but also autoinhibit PPT1 phosphatase activity.

    Sequence similaritiesi

    Contains 3 TPR repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, TPR repeat

    Phylogenomic databases

    GeneTreeiENSGT00530000063173.
    HOGENOMiHOG000172698.
    InParanoidiP53043.
    KOiK04460.
    OMAiCKYKYSQ.
    OrthoDBiEOG092C1UGR.

    Family and domain databases

    Gene3Di1.25.40.10. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_ApaH.
    IPR029052. Metallo-depent_PP-like.
    IPR013235. PPP_dom.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR011236. Ser/Thr_PPase_5.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical_dom.
    IPR019734. TPR_repeat.
    [Graphical view]
    PANTHERiPTHR11668:SF21. PTHR11668:SF21. 3 hits.
    PfamiPF00149. Metallophos. 1 hit.
    PF08321. PPP5. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    SM00028. TPR. 3 hits.
    [Graphical view]
    SUPFAMiSSF48452. SSF48452. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    PS50005. TPR. 3 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53043-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSTPTAADRA KALERKNEGN VFVKEKHFLK AIEKYTEAID LDSTQSIYFS
    60 70 80 90 100
    NRAFAHFKVD NFQSALNDCD EAIKLDPKNI KAYHRRALSC MALLEFKKAR
    110 120 130 140 150
    KDLNVLLKAK PNDPAATKAL LTCDRFIREE RFRKAIGGAE NEAKISLCQT
    160 170 180 190 200
    LNLSSFDANA DLANYEGPKL EFEQLYDDKN AFKGAKIKNM SQEFISKMVN
    210 220 230 240 250
    DLFLKGKYLP KKYVAAIISH ADTLFRQEPS MVELENNSTP DVKISVCGDT
    260 270 280 290 300
    HGQFYDVLNL FRKFGKVGPK HTYLFNGDFV DRGSWSCEVA LLFYCLKILH
    310 320 330 340 350
    PNNFFLNRGN HESDNMNKIY GFEDECKYKY SQRIFNMFAQ SFESLPLATL
    360 370 380 390 400
    INNDYLVMHG GLPSDPSATL SDFKNIDRFA QPPRDGAFME LLWADPQEAN
    410 420 430 440 450
    GMGPSQRGLG HAFGPDITDR FLRNNKLRKI FRSHELRMGG VQFEQKGKLM
    460 470 480 490 500
    TVFSAPNYCD SQGNLGGVIH VVPGHGILQA GRNDDQNLII ETFEAVEHPD
    510
    IKPMAYSNGG FGL
    Length:513
    Mass (Da):57,995
    Last modified:October 1, 1996 - v1
    Checksum:i6966DA22340A5793
    GO

    Sequence cautioni

    The sequence CAA61596 differs from that shown. Reason: Frameshift at position 381.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X89417 Genomic DNA. Translation: CAA61596.1. Frameshift.
    X83099 Genomic DNA. Translation: CAA58158.1.
    Z72908 Genomic DNA. Translation: CAA97134.1.
    AY558095 Genomic DNA. Translation: AAS56421.1.
    S39959 Genomic DNA. Translation: AAB22462.1.
    BK006941 Genomic DNA. Translation: DAA08216.1.
    PIRiS52571.
    RefSeqiNP_011639.3. NM_001181252.3.

    Genome annotation databases

    EnsemblFungiiYGR123C; YGR123C; YGR123C.
    GeneIDi853023.
    KEGGisce:YGR123C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X89417 Genomic DNA. Translation: CAA61596.1. Frameshift.
    X83099 Genomic DNA. Translation: CAA58158.1.
    Z72908 Genomic DNA. Translation: CAA97134.1.
    AY558095 Genomic DNA. Translation: AAS56421.1.
    S39959 Genomic DNA. Translation: AAB22462.1.
    BK006941 Genomic DNA. Translation: DAA08216.1.
    PIRiS52571.
    RefSeqiNP_011639.3. NM_001181252.3.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3ICFX-ray2.30A/B179-513[»]
    ProteinModelPortaliP53043.
    SMRiP53043.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi33370. 30 interactors.
    DIPiDIP-1525N.
    IntActiP53043. 10 interactors.
    MINTiMINT-406578.

    Proteomic databases

    MaxQBiP53043.
    PRIDEiP53043.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYGR123C; YGR123C; YGR123C.
    GeneIDi853023.
    KEGGisce:YGR123C.

    Organism-specific databases

    EuPathDBiFungiDB:YGR123C.
    SGDiS000003355. PPT1.

    Phylogenomic databases

    GeneTreeiENSGT00530000063173.
    HOGENOMiHOG000172698.
    InParanoidiP53043.
    KOiK04460.
    OMAiCKYKYSQ.
    OrthoDBiEOG092C1UGR.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30830-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP53043.
    PROiP53043.

    Family and domain databases

    Gene3Di1.25.40.10. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_ApaH.
    IPR029052. Metallo-depent_PP-like.
    IPR013235. PPP_dom.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR011236. Ser/Thr_PPase_5.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical_dom.
    IPR019734. TPR_repeat.
    [Graphical view]
    PANTHERiPTHR11668:SF21. PTHR11668:SF21. 3 hits.
    PfamiPF00149. Metallophos. 1 hit.
    PF08321. PPP5. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    SM00028. TPR. 3 hits.
    [Graphical view]
    SUPFAMiSSF48452. SSF48452. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    PS50005. TPR. 3 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPPT1_YEAST
    AccessioniPrimary (citable) accession number: P53043
    Secondary accession number(s): D6VUQ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: November 30, 2016
    This is version 159 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6990 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.