Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription initiation factor TFIID subunit 6

Gene

TAF6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.10 Publications

Miscellaneous

Present with 6506 (+/-1290) molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • chromatin binding Source: SGD
  • identical protein binding Source: IntAct
  • protein complex scaffold activity Source: SGD
  • protein heterodimerization activity Source: InterPro
  • RNA polymerase II activating transcription factor binding Source: SGD

GO - Biological processi

  • chromatin organization Source: SGD
  • histone acetylation Source: SGD
  • regulation of sequence-specific DNA binding transcription factor activity Source: InterPro
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30610-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 6
Alternative name(s):
TBP-associated factor 6
TBP-associated factor 60 kDa
Short name:
TAFII-60
Short name:
TAFII60
Gene namesi
Name:TAF6
Synonyms:TAF60
Ordered Locus Names:YGL112C
ORF Names:G2985
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL112C.
SGDiS000003080. TAF6.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188781 – 516Transcription initiation factor TFIID subunit 6Add BLAST516

Proteomic databases

MaxQBiP53040.
PRIDEiP53040.

Interactioni

Subunit structurei

In TFIID, TAF6 heterodimerizes with TAF9, forming ultimately an octamer consisting of a TAF6/TAF9 heterotetramer core flanked by TAF4/TAF12 dimers on either side, similar to the histone H2A/H2B/H3/H4 octamer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Component of the 1.8 MDa SAGA complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA. Component of the SALSA complex, which consists of at least TRA1, SPT7 (C-terminal truncated form), TAF5, ADA3, SPT20, TAF12, TAF6, HFI1, GCN5, ADA2 and SPT3. Component of the SLIK complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SPT20, RTG2, TAF12, TAF6, HFI1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10 and TAF9.6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein complex scaffold activity Source: SGD
  • protein heterodimerization activity Source: InterPro
  • RNA polymerase II activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi33139. 407 interactors.
DIPiDIP-981N.
IntActiP53040. 122 interactors.
MINTiMINT-405577.
STRINGi4932.YGL112C.

Structurei

3D structure databases

ProteinModelPortaliP53040.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 75Histone-foldAdd BLAST70

Sequence similaritiesi

Belongs to the TAF6 family.Curated

Phylogenomic databases

GeneTreeiENSGT00640000091486.
HOGENOMiHOG000159894.
InParanoidiP53040.
KOiK03131.
OMAiDVEYRIH.
OrthoDBiEOG092C0TYK.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR009072. Histone-fold.
IPR011442. TAF6_C.
IPR004823. TAF_TATA-bd.
PfamiView protein in Pfam
PF02969. TAF. 1 hit.
PF07571. TAF6_C. 1 hit.
SMARTiView protein in SMART
SM00803. TAF. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.
SSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

P53040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTQQQSYTI WSPQDTVKDV AESLGLENIN DDVLKALAMD VEYRILEIIE
60 70 80 90 100
QAVKFKRHSK RDVLTTDDVS KALRVLNVEP LYGYYDGSEV NKAVSFSKVN
110 120 130 140 150
TSGGQSVYYL DEEEVDFDRL INEPLPQVPR LPTFTTHWLA VEGVQPAIIQ
160 170 180 190 200
NPNLNDIRVS QPPFIRGAIV TALNDNSLQT PVTSTTASAS VTDTGASQHL
210 220 230 240 250
SNVKPGQNTE VKPLVKHVLS KELQIYFNKV ISTLTAKSQA DEAAQHMKQA
260 270 280 290 300
ALTSLRTDSG LHQLVPYFIQ FIAEQITQNL SDLQLLTTIL EMIYSLLSNT
310 320 330 340 350
SIFLDPYIHS LMPSILTLLL AKKLGGSPKD DSPQEIHEFL ERTNALRDFA
360 370 380 390 400
ASLLDYVLKK FPQAYKSLKP RVTRTLLKTF LDINRVFGTY YGCLKGVSVL
410 420 430 440 450
EGESIRFFLG NLNNWARLVF NESGITLDNI EEHLNDDSNP TRTKFTKEET
460 470 480 490 500
QILVDTVISA LLVLKKDLPD LYEGKGEKVT DEDKEKLLER CGVTIGFHIL
510
KRDDAKELIS AIFFGE
Length:516
Mass (Da):57,902
Last modified:October 1, 1996 - v1
Checksum:i14B590E02C16F289
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97644 Genomic DNA. Translation: CAA66240.1.
L40145 Genomic DNA. Translation: AAA83389.1.
Z72634 Genomic DNA. Translation: CAA96819.1.
AY723809 Genomic DNA. Translation: AAU09726.1.
BK006941 Genomic DNA. Translation: DAA07996.1.
PIRiS64120.
RefSeqiNP_011403.1. NM_001180977.1.

Genome annotation databases

EnsemblFungiiYGL112C; YGL112C; YGL112C.
GeneIDi852766.
KEGGisce:YGL112C.

Similar proteinsi

Entry informationi

Entry nameiTAF6_YEAST
AccessioniPrimary (citable) accession number: P53040
Secondary accession number(s): D6VU35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names