Protein transport protein YIP1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P53039 (YIP1_YEAST)

Last modified February 9, 2010. Version 74. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Protein transport protein YIP1
Alternative name(s):
YPT-interacting protein 1

Gene names

Name:	YIP1
Ordered Locus Names:	YGR172C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Required for fusion of ER-derived vesicles with the Golgi during ER-to-Golgi protein transport, probably by mediating correct membrane localization of YPT1. Ref.1 Ref.6
Subunit structure	Component of the YIP1-YIF1 complex, composed of at least YIF1, YIP1 and YOS1. The complex interacts with the ER to Golgi SNAREs BOS1 and SEC22. Interacts with the prenylated form of the Rab GTPases YPT1 and YPT31. Interacts with the YIP1 family members YIP4 and YIP5. Interacts with SNX3, YIP3 and YOP1. Ref.1 Ref.6 Ref.3 Ref.4 Ref.5 Ref.7 Ref.8 Ref.9
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein Ref.1 Ref.3.
Sequence similarities	Belongs to the YIP1 family.

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Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle fusion with Golgi apparatus Ref.6 Inferred from mutant phenotype. Source: SGD
Cellular component	ER to Golgi transport vesicle Inferred from direct assay. Source: SGD endoplasmic reticulum membrane Inferred from direct assay. Source: SGD integral to Golgi membrane Ref.1 Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.5 Ref.9 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ATG20	Q07528	1	EBI-25295,EBI-36894
EEB1	Q02891	1	EBI-25295,EBI-29462
GCS1	P35197	1	EBI-25295,EBI-7475
SNX3	Q08826	1	EBI-25295,EBI-17603
VAM7	P32912	1	EBI-25295,EBI-20232
VPS17	P32913	1	EBI-25295,EBI-20366
YIF1	P53845	3	EBI-25295,EBI-28230
YIP3	P53633	1	EBI-25295,EBI-25301
YIP4	P53093	1	EBI-25295,EBI-24124
YPT35	P38815	2	EBI-25295,EBI-24665

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 248

248

Protein transport protein YIP1

PRO_0000066269

Regions

Topological domain

1 – 110

110

Cytoplasmic Potential

Transmembrane

111 – 131

Potential

Topological domain

132

Lumenal Potential

Transmembrane

133 – 153

Potential

Topological domain

154 – 172

Cytoplasmic Potential

Transmembrane

173 – 193

Potential

Topological domain

194 – 196

Lumenal Potential

Transmembrane

197 – 217

Potential

Topological domain

218 – 227

Cytoplasmic Potential

Transmembrane

228 – 248

Potential

Experimental info

Mutagenesis

E → G in YIP1-41; lethal; abolishes binding to YIF1, YPT1 and YPT31. In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with A-130. Ref.8

Mutagenesis

E → K in YIP1-4; temperature sensitive; reduces binding to YIF1, YPT1 and YPT31. In YIP1-44; lethal; when associated with E-130. Ref.8

Mutagenesis

E → V in YIP1-42; temperature sensitive; abolishes binding to YPT1 and YPT31, and inhibits ER vesicle budding. Ref.8

Mutagenesis

E → K in YIP1-6; lethal; reduces binding to YIF1. Ref.8

Mutagenesis

F → Y in YIP1-9; lethal. Ref.8

Mutagenesis

114

P → L in YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with E-129. Ref.1

Mutagenesis

129

G → E in YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with L-114. Ref.1

Mutagenesis

130

K → A in YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with G-70. Ref.8

Mutagenesis

130

K → E in YIP1-44; lethal; when associated with K-70. Ref.8

Mutagenesis

153 – 155

LMS → GGG in YIP1-12; abolishes binding to YPT1 and YPT31. Ref.8

Mutagenesis

175

G → E in YIP1-2; abolishes binding to YPT1 and YPT31, reduces binding to YIF1, blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius. Ref.1

Mutagenesis

177

C → S in YIP1-18; abolishes binding to YIF1, YPT1 and YPT31. Ref.8

Mutagenesis

180

P → G in YIP1-19; lethal; abolishes binding to YPT1 and YPT31, and reduces binding to YIF1. Ref.8

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Sequences

Sequence

Length

Mass (Da)

Tools

P53039-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 64372B4823A0BC5E
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FASTA

248

27,080

        10         20         30         40         50         60 
MSFYNTSNNA NNGGGFYQPS AQFAVPQGSM SFQNTVGSSN TGNDNNLGVA PDPLPVGILH 

        70         80         90        100        110        120 
ALSTKGYPHE PPLLEEIGIN FDHIITKTKM VLIPIRFGSG VPQEILNDSD LAGPLIFFLL 

       130        140        150        160        170        180 
FGLFLLMAGK VHFGYIYGVA LFGTISLHNL SKLMSNNDTS TQTNLQFFNT ASILGYCFLP 

       190        200        210        220        230        240 
LCFLSLLGIF HGLNNTTGYV VSVLFVIWST WTSSGFLNSL LQLQNARLLI AYPLLIFYSV 


FALMVIFV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p." Yang X., Matern H.T., Gallwitz D. EMBO J. 17:4954-4963(1998) [PubMed: 9724632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH YPT1 AND YPT31, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF PRO-114; GLY-129 AND GLY-175. Strain: HLR3.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"A novel Golgi membrane protein is part of a GTPase-binding protein complex involved in vesicle targeting." Matern H.T., Yang X., Andrulis E., Sternglanz R., Trepte H.-H., Gallwitz D. EMBO J. 19:4485-4492(2000) [PubMed: 10970842] [Abstract] Cited for: INTERACTION WITH YIF1, SUBCELLULAR LOCATION.
[4]	"Yop1p, the yeast homolog of the polyposis locus protein 1, interacts with Yip1p and negatively regulates cell growth." Calero M., Whittaker G.R., Collins R.N. J. Biol. Chem. 276:12100-12112(2001) [PubMed: 11278413] [Abstract] Cited for: INTERACTION WITH YOP1.
[5]	"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins." Calero M., Collins R.N. Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed: 11785952] [Abstract] Cited for: INTERACTION WITH YIP3.
[6]	"The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles." Barrowman J., Wang W., Zhang Y., Ferro-Novick S. J. Biol. Chem. 278:19878-19884(2003) [PubMed: 12657649] [Abstract] Cited for: FUNCTION, INTERACTION WITH BOS1; SEC22 AND YPT1.
[7]	"Dual prenylation is required for Rab protein localization and function." Calero M., Chen C.Z., Zhu W., Winand N.J., Havas K.A., Gilbert P.M., Burd C.G., Collins R.N. Mol. Biol. Cell 14:1852-1867(2003) [PubMed: 12802060] [Abstract] Cited for: INTERACTION WITH YIP4; YIP5 AND YPT1.
[8]	"Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor." Chen C.Z., Calero M., DeRegis C.J., Heidtman M., Barlowe C., Collins R.N. Genetics 168:1827-1841(2004) [PubMed: 15611160] [Abstract] Cited for: MUTAGENESIS OF GLU-70; GLU-76; PHE-81; LYS-130; 153-LEU--SER-155; CYS-177 AND PRO-180, INTERACTION WITH YIF1; YPT1 AND YPT31.
[9]	"The phox homology (PX) domain protein interaction network in yeast." Vollert C.S., Uetz P. Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed: 15263065] [Abstract] Cited for: INTERACTION WITH SNX3.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	X97342 Genomic DNA. Translation: CAA66031.1. Z72957 Genomic DNA. Translation: CAA97198.1.
PIR	S64486.
RefSeq	NP_011688.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2080N.
IntAct	P53039. 20 interactions.
STRING	P53039.
Genome annotation databases
Ensembl	YGR172C; YGR172C; YGR172C; Saccharomyces cerevisiae. [Genome view]
GeneID	853082.
KEGG	sce:YGR172C.
NMPDR	fig\|4932.3.peg.2814.
Organism-specific databases
CYGD	YGR172c.
SGD	S000003404. YIP1.
Phylogenomic databases
eggNOG	fuNOG09767.
HOGENOM	HBG611223.
OMA	LVAYPCV.
OrthoDB	EOG9WQ2KB.
PhylomeDB	P53039.
Gene expression databases
ArrayExpress	P53039.
Genevestigator	P53039.
GermOnline	YGR172C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	973053.

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Entry information

Entry name

YIP1_YEAST

Accession

Primary (citable) accession number: P53039

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents