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P53039 (YIP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein YIP1
Alternative name(s):
YPT-interacting protein 1
Gene names
Name:YIP1
Ordered Locus Names:YGR172C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for fusion of ER-derived vesicles with the Golgi during ER-to-Golgi protein transport, probably by mediating correct membrane localization of YPT1. Ref.1 Ref.7

Subunit structure

Component of the YIP1-YIF1 complex, composed of at least YIF1, YIP1 and YOS1. The complex interacts with the ER to Golgi SNAREs BOS1 and SEC22. Interacts with the prenylated form of the Rab GTPases YPT1 and YPT31. Interacts with the YIP1 family members YIP4 and YIP5. Interacts with SNX3, YIP3 and YOP1. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein Ref.1 Ref.4.

Sequence similarities

Belongs to the YIP1 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Protein transport protein YIP1
PRO_0000066269

Regions

Topological domain1 – 110110Cytoplasmic Potential
Transmembrane111 – 13121Helical; Potential
Topological domain1321Lumenal Potential
Transmembrane133 – 15321Helical; Potential
Topological domain154 – 17219Cytoplasmic Potential
Transmembrane173 – 19321Helical; Potential
Topological domain194 – 1963Lumenal Potential
Transmembrane197 – 21721Helical; Potential
Topological domain218 – 22710Cytoplasmic Potential
Transmembrane228 – 24821Helical; Potential

Experimental info

Mutagenesis701E → G in YIP1-41; lethal; abolishes binding to YIF1, YPT1 and YPT31. In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with A-130. Ref.9
Mutagenesis701E → K in YIP1-4; temperature sensitive; reduces binding to YIF1, YPT1 and YPT31. In YIP1-44; lethal; when associated with E-130. Ref.9
Mutagenesis701E → V in YIP1-42; temperature sensitive; abolishes binding to YPT1 and YPT31, and inhibits ER vesicle budding. Ref.9
Mutagenesis761E → K in YIP1-6; lethal; reduces binding to YIF1. Ref.9
Mutagenesis811F → Y in YIP1-9; lethal. Ref.9
Mutagenesis1141P → L in YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with E-129. Ref.1
Mutagenesis1291G → E in YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with L-114. Ref.1
Mutagenesis1301K → A in YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with G-70. Ref.9
Mutagenesis1301K → E in YIP1-44; lethal; when associated with K-70. Ref.9
Mutagenesis153 – 1553LMS → GGG in YIP1-12; abolishes binding to YPT1 and YPT31. Ref.9
Mutagenesis1751G → E in YIP1-2; abolishes binding to YPT1 and YPT31, reduces binding to YIF1, blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius. Ref.1
Mutagenesis1771C → S in YIP1-18; abolishes binding to YIF1, YPT1 and YPT31. Ref.9
Mutagenesis1801P → G in YIP1-19; lethal; abolishes binding to YPT1 and YPT31, and reduces binding to YIF1. Ref.9

Sequences

Sequence LengthMass (Da)Tools
P53039 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 64372B4823A0BC5E

FASTA24827,080
        10         20         30         40         50         60 
MSFYNTSNNA NNGGGFYQPS AQFAVPQGSM SFQNTVGSSN TGNDNNLGVA PDPLPVGILH 

        70         80         90        100        110        120 
ALSTKGYPHE PPLLEEIGIN FDHIITKTKM VLIPIRFGSG VPQEILNDSD LAGPLIFFLL 

       130        140        150        160        170        180 
FGLFLLMAGK VHFGYIYGVA LFGTISLHNL SKLMSNNDTS TQTNLQFFNT ASILGYCFLP 

       190        200        210        220        230        240 
LCFLSLLGIF HGLNNTTGYV VSVLFVIWST WTSSGFLNSL LQLQNARLLI AYPLLIFYSV 


FALMVIFV 

« Hide

References

« Hide 'large scale' references
[1]"Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p."
Yang X., Matern H.T., Gallwitz D.
EMBO J. 17:4954-4963(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH YPT1 AND YPT31, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF PRO-114; GLY-129 AND GLY-175.
Strain: HLR3.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A novel Golgi membrane protein is part of a GTPase-binding protein complex involved in vesicle targeting."
Matern H.T., Yang X., Andrulis E., Sternglanz R., Trepte H.-H., Gallwitz D.
EMBO J. 19:4485-4492(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIF1, SUBCELLULAR LOCATION.
[5]"Yop1p, the yeast homolog of the polyposis locus protein 1, interacts with Yip1p and negatively regulates cell growth."
Calero M., Whittaker G.R., Collins R.N.
J. Biol. Chem. 276:12100-12112(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YOP1.
[6]"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
Calero M., Collins R.N.
Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIP3.
[7]"The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles."
Barrowman J., Wang W., Zhang Y., Ferro-Novick S.
J. Biol. Chem. 278:19878-19884(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BOS1; SEC22 AND YPT1.
[8]"Dual prenylation is required for Rab protein localization and function."
Calero M., Chen C.Z., Zhu W., Winand N.J., Havas K.A., Gilbert P.M., Burd C.G., Collins R.N.
Mol. Biol. Cell 14:1852-1867(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIP4; YIP5 AND YPT1.
[9]"Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor."
Chen C.Z., Calero M., DeRegis C.J., Heidtman M., Barlowe C., Collins R.N.
Genetics 168:1827-1841(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF GLU-70; GLU-76; PHE-81; LYS-130; 153-LEU--SER-155; CYS-177 AND PRO-180, INTERACTION WITH YIF1; YPT1 AND YPT31.
[10]"The phox homology (PX) domain protein interaction network in yeast."
Vollert C.S., Uetz P.
Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNX3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X97342 Genomic DNA. Translation: CAA66031.1.
Z72957 Genomic DNA. Translation: CAA97198.1.
BK006941 Genomic DNA. Translation: DAA08267.1.
PIRS64486.
RefSeqNP_011688.3. NM_001181301.3.

3D structure databases

ProteinModelPortalP53039.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33424. 44 interactions.
DIPDIP-2080N.
IntActP53039. 12 interactions.
MINTMINT-480951.
STRING4932.YGR172C.

Protein family/group databases

TCDB9.B.135.1.1. the membrane trafficking yip (yip) family.

Proteomic databases

PaxDbP53039.

Protocols and materials databases

DNASU853082.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR172C; YGR172C; YGR172C.
GeneID853082.
KEGGsce:YGR172C.

Organism-specific databases

CYGDYGR172c.
SGDS000003404. YIP1.

Phylogenomic databases

eggNOGCOG5080.
GeneTreeENSGT00510000046817.
HOGENOMHOG000037706.
OMAGGWWTAF.
OrthoDBEOG7KM654.

Enzyme and pathway databases

BioCycYEAST:G3O-30868-MONOMER.

Gene expression databases

GenevestigatorP53039.

Family and domain databases

InterProIPR006977. Yip1.
[Graphical view]
PfamPF04893. Yip1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973053.
PROP53039.

Entry information

Entry nameYIP1_YEAST
AccessionPrimary (citable) accession number: P53039
Secondary accession number(s): D6VUV6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families