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P53039

- YIP1_YEAST

UniProt

P53039 - YIP1_YEAST

Protein

Protein transport protein YIP1

Gene

YIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Required for fusion of ER-derived vesicles with the Golgi during ER-to-Golgi protein transport, probably by mediating correct membrane localization of YPT1.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: SGD
    2. protein transport Source: UniProtKB-KW
    3. vesicle fusion with Golgi apparatus Source: SGD
    4. vesicle-mediated transport Source: SGD

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30868-MONOMER.

    Protein family/group databases

    TCDBi9.B.135.1.1. the membrane trafficking yip (yip) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein transport protein YIP1
    Alternative name(s):
    YPT-interacting protein 1
    Gene namesi
    Name:YIP1
    Ordered Locus Names:YGR172C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR172c.
    SGDiS000003404. YIP1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: SGD
    2. ER to Golgi transport vesicle Source: SGD
    3. integral component of Golgi membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi70 – 701E → G in YIP1-41; lethal; abolishes binding to YIF1, YPT1 and YPT31. In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with A-130. 1 Publication
    Mutagenesisi70 – 701E → K in YIP1-4; temperature sensitive; reduces binding to YIF1, YPT1 and YPT31. In YIP1-44; lethal; when associated with E-130. 1 Publication
    Mutagenesisi70 – 701E → V in YIP1-42; temperature sensitive; abolishes binding to YPT1 and YPT31, and inhibits ER vesicle budding. 1 Publication
    Mutagenesisi76 – 761E → K in YIP1-6; lethal; reduces binding to YIF1. 1 Publication
    Mutagenesisi81 – 811F → Y in YIP1-9; lethal. 1 Publication
    Mutagenesisi114 – 1141P → L in YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with E-129. 1 Publication
    Mutagenesisi129 – 1291G → E in YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with L-114. 1 Publication
    Mutagenesisi130 – 1301K → A in YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with G-70. 1 Publication
    Mutagenesisi130 – 1301K → E in YIP1-44; lethal; when associated with K-70. 1 Publication
    Mutagenesisi153 – 1553LMS → GGG in YIP1-12; abolishes binding to YPT1 and YPT31.
    Mutagenesisi175 – 1751G → E in YIP1-2; abolishes binding to YPT1 and YPT31, reduces binding to YIF1, blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius. 1 Publication
    Mutagenesisi177 – 1771C → S in YIP1-18; abolishes binding to YIF1, YPT1 and YPT31. 1 Publication
    Mutagenesisi180 – 1801P → G in YIP1-19; lethal; abolishes binding to YPT1 and YPT31, and reduces binding to YIF1. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 248248Protein transport protein YIP1PRO_0000066269Add
    BLAST

    Proteomic databases

    MaxQBiP53039.
    PaxDbiP53039.

    Expressioni

    Gene expression databases

    GenevestigatoriP53039.

    Interactioni

    Subunit structurei

    Component of the YIP1-YIF1 complex, composed of at least YIF1, YIP1 and YOS1. The complex interacts with the ER to Golgi SNAREs BOS1 and SEC22. Interacts with the prenylated form of the Rab GTPases YPT1 and YPT31. Interacts with the YIP1 family members YIP4 and YIP5. Interacts with SNX3, YIP3 and YOP1.8 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ATG20Q075282EBI-25295,EBI-36894
    VPS17P329132EBI-25295,EBI-20366
    YIF1P538456EBI-25295,EBI-28230
    YPT35P388153EBI-25295,EBI-24665

    Protein-protein interaction databases

    BioGridi33424. 44 interactions.
    DIPiDIP-2080N.
    IntActiP53039. 12 interactions.
    MINTiMINT-480951.
    STRINGi4932.YGR172C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53039.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 110110CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini132 – 1321LumenalSequence Analysis
    Topological domaini154 – 17219CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini194 – 1963LumenalSequence Analysis
    Topological domaini218 – 22710CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei111 – 13121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei133 – 15321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei173 – 19321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei197 – 21721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei228 – 24821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the YIP1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5080.
    GeneTreeiENSGT00510000046817.
    HOGENOMiHOG000037706.
    OMAiGGWWTAF.
    OrthoDBiEOG7KM654.

    Family and domain databases

    InterProiIPR006977. Yip1.
    [Graphical view]
    PfamiPF04893. Yip1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53039-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFYNTSNNA NNGGGFYQPS AQFAVPQGSM SFQNTVGSSN TGNDNNLGVA    50
    PDPLPVGILH ALSTKGYPHE PPLLEEIGIN FDHIITKTKM VLIPIRFGSG 100
    VPQEILNDSD LAGPLIFFLL FGLFLLMAGK VHFGYIYGVA LFGTISLHNL 150
    SKLMSNNDTS TQTNLQFFNT ASILGYCFLP LCFLSLLGIF HGLNNTTGYV 200
    VSVLFVIWST WTSSGFLNSL LQLQNARLLI AYPLLIFYSV FALMVIFV 248
    Length:248
    Mass (Da):27,080
    Last modified:October 1, 1996 - v1
    Checksum:i64372B4823A0BC5E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97342 Genomic DNA. Translation: CAA66031.1.
    Z72957 Genomic DNA. Translation: CAA97198.1.
    BK006941 Genomic DNA. Translation: DAA08267.1.
    PIRiS64486.
    RefSeqiNP_011688.3. NM_001181301.3.

    Genome annotation databases

    EnsemblFungiiYGR172C; YGR172C; YGR172C.
    GeneIDi853082.
    KEGGisce:YGR172C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97342 Genomic DNA. Translation: CAA66031.1 .
    Z72957 Genomic DNA. Translation: CAA97198.1 .
    BK006941 Genomic DNA. Translation: DAA08267.1 .
    PIRi S64486.
    RefSeqi NP_011688.3. NM_001181301.3.

    3D structure databases

    ProteinModelPortali P53039.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33424. 44 interactions.
    DIPi DIP-2080N.
    IntActi P53039. 12 interactions.
    MINTi MINT-480951.
    STRINGi 4932.YGR172C.

    Protein family/group databases

    TCDBi 9.B.135.1.1. the membrane trafficking yip (yip) family.

    Proteomic databases

    MaxQBi P53039.
    PaxDbi P53039.

    Protocols and materials databases

    DNASUi 853082.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR172C ; YGR172C ; YGR172C .
    GeneIDi 853082.
    KEGGi sce:YGR172C.

    Organism-specific databases

    CYGDi YGR172c.
    SGDi S000003404. YIP1.

    Phylogenomic databases

    eggNOGi COG5080.
    GeneTreei ENSGT00510000046817.
    HOGENOMi HOG000037706.
    OMAi GGWWTAF.
    OrthoDBi EOG7KM654.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30868-MONOMER.

    Miscellaneous databases

    NextBioi 973053.
    PROi P53039.

    Gene expression databases

    Genevestigatori P53039.

    Family and domain databases

    InterProi IPR006977. Yip1.
    [Graphical view ]
    Pfami PF04893. Yip1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p."
      Yang X., Matern H.T., Gallwitz D.
      EMBO J. 17:4954-4963(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH YPT1 AND YPT31, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF PRO-114; GLY-129 AND GLY-175.
      Strain: HLR3.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A novel Golgi membrane protein is part of a GTPase-binding protein complex involved in vesicle targeting."
      Matern H.T., Yang X., Andrulis E., Sternglanz R., Trepte H.-H., Gallwitz D.
      EMBO J. 19:4485-4492(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIF1, SUBCELLULAR LOCATION.
    5. "Yop1p, the yeast homolog of the polyposis locus protein 1, interacts with Yip1p and negatively regulates cell growth."
      Calero M., Whittaker G.R., Collins R.N.
      J. Biol. Chem. 276:12100-12112(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YOP1.
    6. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
      Calero M., Collins R.N.
      Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIP3.
    7. "The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles."
      Barrowman J., Wang W., Zhang Y., Ferro-Novick S.
      J. Biol. Chem. 278:19878-19884(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH BOS1; SEC22 AND YPT1.
    8. "Dual prenylation is required for Rab protein localization and function."
      Calero M., Chen C.Z., Zhu W., Winand N.J., Havas K.A., Gilbert P.M., Burd C.G., Collins R.N.
      Mol. Biol. Cell 14:1852-1867(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIP4; YIP5 AND YPT1.
    9. "Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor."
      Chen C.Z., Calero M., DeRegis C.J., Heidtman M., Barlowe C., Collins R.N.
      Genetics 168:1827-1841(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF GLU-70; GLU-76; PHE-81; LYS-130; 153-LEU--SER-155; CYS-177 AND PRO-180, INTERACTION WITH YIF1; YPT1 AND YPT31.
    10. "The phox homology (PX) domain protein interaction network in yeast."
      Vollert C.S., Uetz P.
      Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNX3.

    Entry informationi

    Entry nameiYIP1_YEAST
    AccessioniPrimary (citable) accession number: P53039
    Secondary accession number(s): D6VUV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3