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Protein

Protein transport protein YIP1

Gene

YIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for fusion of ER-derived vesicles with the Golgi during ER-to-Golgi protein transport, probably by mediating correct membrane localization of YPT1.2 Publications

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • protein transport Source: UniProtKB-KW
  • vesicle fusion with Golgi apparatus Source: SGD
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30868-MONOMER.

Protein family/group databases

TCDBi9.B.135.1.1. the membrane trafficking yip (yip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein YIP1
Alternative name(s):
YPT-interacting protein 1
Gene namesi
Name:YIP1
Ordered Locus Names:YGR172C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR172C.
SGDiS000003404. YIP1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 110CytoplasmicSequence analysisAdd BLAST110
Transmembranei111 – 131HelicalSequence analysisAdd BLAST21
Topological domaini132LumenalSequence analysis1
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Topological domaini154 – 172CytoplasmicSequence analysisAdd BLAST19
Transmembranei173 – 193HelicalSequence analysisAdd BLAST21
Topological domaini194 – 196LumenalSequence analysis3
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Topological domaini218 – 227CytoplasmicSequence analysis10
Transmembranei228 – 248HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • ER to Golgi transport vesicle Source: SGD
  • integral component of Golgi membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi70E → G in YIP1-41; lethal; abolishes binding to YIF1, YPT1 and YPT31. In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with A-130. 1 Publication1
Mutagenesisi70E → K in YIP1-4; temperature sensitive; reduces binding to YIF1, YPT1 and YPT31. In YIP1-44; lethal; when associated with E-130. 1 Publication1
Mutagenesisi70E → V in YIP1-42; temperature sensitive; abolishes binding to YPT1 and YPT31, and inhibits ER vesicle budding. 1 Publication1
Mutagenesisi76E → K in YIP1-6; lethal; reduces binding to YIF1. 1 Publication1
Mutagenesisi81F → Y in YIP1-9; lethal. 1 Publication1
Mutagenesisi114P → L in YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with E-129. 1 Publication1
Mutagenesisi129G → E in YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with L-114. 1 Publication1
Mutagenesisi130K → A in YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with G-70. 1 Publication1
Mutagenesisi130K → E in YIP1-44; lethal; when associated with K-70. 1 Publication1
Mutagenesisi153 – 155LMS → GGG in YIP1-12; abolishes binding to YPT1 and YPT31. 1 Publication3
Mutagenesisi175G → E in YIP1-2; abolishes binding to YPT1 and YPT31, reduces binding to YIF1, blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius. 1 Publication1
Mutagenesisi177C → S in YIP1-18; abolishes binding to YIF1, YPT1 and YPT31. 1 Publication1
Mutagenesisi180P → G in YIP1-19; lethal; abolishes binding to YPT1 and YPT31, and reduces binding to YIF1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000662691 – 248Protein transport protein YIP1Add BLAST248

Proteomic databases

MaxQBiP53039.
PRIDEiP53039.

Interactioni

Subunit structurei

Component of the YIP1-YIF1 complex, composed of at least YIF1, YIP1 and YOS1. The complex interacts with the ER to Golgi SNAREs BOS1 and SEC22. Interacts with the prenylated form of the Rab GTPases YPT1 and YPT31. Interacts with the YIP1 family members YIP4 and YIP5. Interacts with SNX3, YIP3 and YOP1.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG20Q075282EBI-25295,EBI-36894
VPS17P329132EBI-25295,EBI-20366
YIF1P538456EBI-25295,EBI-28230
YPT35P388153EBI-25295,EBI-24665

Protein-protein interaction databases

BioGridi33424. 49 interactors.
DIPiDIP-2080N.
IntActiP53039. 12 interactors.
MINTiMINT-480951.

Structurei

3D structure databases

ProteinModelPortaliP53039.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the YIP1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046817.
HOGENOMiHOG000037706.
InParanoidiP53039.
KOiK20363.
OMAiMLMSSGY.
OrthoDBiEOG092C580G.

Family and domain databases

InterProiIPR006977. Yip1.
[Graphical view]
PfamiPF04893. Yip1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFYNTSNNA NNGGGFYQPS AQFAVPQGSM SFQNTVGSSN TGNDNNLGVA
60 70 80 90 100
PDPLPVGILH ALSTKGYPHE PPLLEEIGIN FDHIITKTKM VLIPIRFGSG
110 120 130 140 150
VPQEILNDSD LAGPLIFFLL FGLFLLMAGK VHFGYIYGVA LFGTISLHNL
160 170 180 190 200
SKLMSNNDTS TQTNLQFFNT ASILGYCFLP LCFLSLLGIF HGLNNTTGYV
210 220 230 240
VSVLFVIWST WTSSGFLNSL LQLQNARLLI AYPLLIFYSV FALMVIFV
Length:248
Mass (Da):27,080
Last modified:October 1, 1996 - v1
Checksum:i64372B4823A0BC5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97342 Genomic DNA. Translation: CAA66031.1.
Z72957 Genomic DNA. Translation: CAA97198.1.
BK006941 Genomic DNA. Translation: DAA08267.1.
PIRiS64486.
RefSeqiNP_011688.3. NM_001181301.3.

Genome annotation databases

EnsemblFungiiYGR172C; YGR172C; YGR172C.
GeneIDi853082.
KEGGisce:YGR172C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97342 Genomic DNA. Translation: CAA66031.1.
Z72957 Genomic DNA. Translation: CAA97198.1.
BK006941 Genomic DNA. Translation: DAA08267.1.
PIRiS64486.
RefSeqiNP_011688.3. NM_001181301.3.

3D structure databases

ProteinModelPortaliP53039.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33424. 49 interactors.
DIPiDIP-2080N.
IntActiP53039. 12 interactors.
MINTiMINT-480951.

Protein family/group databases

TCDBi9.B.135.1.1. the membrane trafficking yip (yip) family.

Proteomic databases

MaxQBiP53039.
PRIDEiP53039.

Protocols and materials databases

DNASUi853082.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR172C; YGR172C; YGR172C.
GeneIDi853082.
KEGGisce:YGR172C.

Organism-specific databases

EuPathDBiFungiDB:YGR172C.
SGDiS000003404. YIP1.

Phylogenomic databases

GeneTreeiENSGT00510000046817.
HOGENOMiHOG000037706.
InParanoidiP53039.
KOiK20363.
OMAiMLMSSGY.
OrthoDBiEOG092C580G.

Enzyme and pathway databases

BioCyciYEAST:G3O-30868-MONOMER.

Miscellaneous databases

PROiP53039.

Family and domain databases

InterProiIPR006977. Yip1.
[Graphical view]
PfamiPF04893. Yip1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYIP1_YEAST
AccessioniPrimary (citable) accession number: P53039
Secondary accession number(s): D6VUV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.