Reviewed, UniProtKB/Swiss-Prot P53037 (PSD2_YEAST)

Names and origin

Protein names

Recommended name:
    Phosphatidylserine decarboxylase proenzyme 2
    EC=4.1.1.65
Cleaved into the following 2 chains:
    1- Recommended name:
            Phosphatidylserine decarboxylase 2 beta chain
    2- Recommended name:
            Phosphatidylserine decarboxylase 2 alpha chain

Gene names

Name:	PSD2
Ordered Locus Names:	YGR170W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the regulation of phospholipid biosynthesis and interorganelle trafficking of phosphatidylserine.
Catalytic activity	Phosphatidyl-L-serine = phosphatidylethanolamine + CO2.
Cofactor	Pyruvoyl group.
Pathway	Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.
Subcellular location	Golgi apparatus › Golgi stack. Vacuole.
Sequence similarities	Belongs to the phosphatidylserine decarboxylase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Golgi apparatus Vacuole
Ligand	Pyruvate
Molecular function	Decarboxylase Lyase
PTM	Phosphoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphatidylcholine biosynthetic process Inferred from direct assay. Source: SGD
Cellular component	Golgi membrane Traceable author statement. Source: SGD Golgi stack Inferred from electronic annotation. Source: UniProtKB-SubCell fungal-type vacuole membrane Traceable author statement. Source: SGD
Molecular function	phosphatidylserine decarboxylase activity Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1138	1138	Phosphatidylserine decarboxylase proenzyme 2		PRO_0000045200
	Chain	1 – 1042	1042	Phosphatidylserine decarboxylase 2 beta chain By similarity		PRO_0000045201
	Chain	1043 – 1138	96	Phosphatidylserine decarboxylase 2 alpha chain By similarity		PRO_0000045202
Regions
	Compositional bias	152 – 159	8	Poly-Ser
	Compositional bias	271 – 274	4	Poly-Ser
	Compositional bias	591 – 594	4	Poly-Gln
	Compositional bias	1045 – 1049	5	Poly-Ile
Sites
	Site	1042 – 1043	2	Cleavage (non-hydrolytic) By similarity
Amino acid modifications
	Modified residue	1043	1	Pyruvic acid (Ser) By similarity
	Modified residue	1108	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	801	1	E → G in AAA69819. Ref.1
	Sequence conflict	974	1	Y → N in AAA69819. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53037-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 934BA6579E121053	FASTA	1,138	130,065
	10 20 30 40 50 60 MRIIKGRKRG KNKKPTLILK IHVIQAENIE ALKTFNCNPV CFVTTNTFYS QKTNKLKNSN 70 80 90 100 110 120 THWNQTLRIK LPRNPTSEWL RIIVYDALPT GAPPTTPSRP RTTTANTSSS TLSNSGLSSH 130 140 150 160 170 180 SHSSRNLNVT SKGNQTSTSI NSVSSSATPA PSHSSSSLST TGPGSTHKNR INSYLYLGEA 190 200 210 220 230 240 KISLLDLFKR KDTTTSYKFS IEAQRYHLYD MKGGKDQDSL NCNFLVGDIL LGFKLECNVK 250 260 270 280 290 300 RTPTFQAFNA WRNELNTYLG RIDRNKARMR SSSSLPPPLE DMLSNSSAVS GNEIRREKPY 310 320 330 340 350 360 SDTDLAHDEE VNAEDEIDAE ESIEDMNSSG SICTERRYDI DNDTIFDSIS EVVSLNDEEL 370 380 390 400 410 420 DILNDFEEAD HPNVPDINVH DIDEDTRISL SSMITALDEY DIVEPEDVAK LPAVSENDIT 430 440 450 460 470 480 SVDDEESENQ QESDEEFDIY NEDEREDSDF QSKEYIGSRL LHLQRGKHNK SYANYLYRRA 490 500 510 520 530 540 KSNFFISKKE HAMGVVFMHI GAIKNLPALR NRLSKTNYEM DPFIVISFGR RVFKTSWRKH 550 560 570 580 590 600 TLNPEFNEYA AFEVFPHETN FAFSIKVVDK DSFSFNDDVA KCELAWFDML QQQQHENEWI 610 620 630 640 650 660 PYEIPLDLTV EPAHAPKQPV LYSSFKYVSY PFLKKSFWKE AVDTSVNLER LDIIQVMLYL 670 680 690 700 710 720 ERLGSFTMAD SFELFQHFNK SAWAGQSITR SQLVEGLQSW RKSTNFKRIW TCPRCMRSCK 730 740 750 760 770 780 PTRNARRSKL VLENDLITHF AICTFSKEHK TLKPSYVSSA FASKRWFSKV LIKLTYGKYA 790 800 810 820 830 840 LGSNNANILV QDRDTGIIIE EKISAHVKLG MRIIYNGKSP ESKKFRSLLK TLSIRQGKKF 850 860 870 880 890 900 DSTASAKQIE PFIKFHSLDL SQCRDKDFKT FNEFFYRKLK PGSRLPESNN KEILFSPADS 910 920 930 940 950 960 RCTVFPTIQE SKEIWVKGRK FSIKKLANNY NPETFNDNNC SIGIFRLAPQ DYHRFHSPCN 970 980 990 1000 1010 1020 GTIGKPVYVD GEYYTVNPMA VRSELDVFGE NIRVIIPIDS PQFGKLLYIP IGAMMVGSIL 1030 1040 1050 1060 1070 1080 LTCKENDVVE SGQELGYFKF GGSTIIIIIP HNNFMFDSDL VKNSSERIET LVKVGMSIGH 1090 1100 1110 1120 1130 TSNVNELKRI RIKVDDPKKI ERIKRTISVS DENAKSTGNV TWEYHTLREM MNKDFAGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phosphatidylserine decarboxylase 2 of Saccharomyces cerevisiae. Cloning and mapping of the gene, heterologous expression, and creation of the null allele." Trotter P.J., Pedretti J., Yates R., Voelker D.R. J. Biol. Chem. 270:6071-6080(1995) [PubMed: 7890740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PTY36.
[2]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1108, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U19910 Genomic DNA. Translation: AAA69819.1. Z72955 Genomic DNA. Translation: CAA97196.1.
PIR	S64484.
RefSeq	NP_011686.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6756N.
IntAct	P53037. 9 interactions.
STRING	P53037.
Proteomic databases
PRIDE	P53037.
Genome annotation databases
Ensembl	YGR170W; YGR170W; YGR170W; Saccharomyces cerevisiae. [Genome view]
GeneID	853080.
GenomeReviews	Gene locus YGR170W in contig Y13135_GR.
KEGG	sce:YGR170W.
NMPDR	fig|4932.3.peg.2812.
Organism-specific databases
CYGD	YGR170w.
SGD	S000003402. PSD2.
Phylogenomic databases
HOGENOM	P53037.
OMA	MRIIYNG.
Enzyme and pathway databases
BRENDA	4.1.1.65. 250.
Gene expression databases
ArrayExpress	P53037.
Genevestigator	P53037.
GermOnline	YGR170W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR003817. PS_Dcarbxylase. IPR005221. PS_decarb. [Graphical view]
PANTHER	PTHR10067. PS_decarb. 1 hit.
Pfam	PF00168. C2. 2 hits. PF02666. PS_Dcarbxylase. 1 hit. [Graphical view]
SMART	SM00239. C2. 2 hits. [Graphical view]
TIGRFAMs	TIGR00163. PS_decarb. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	973047.

Entry information

Entry name

PSD2_YEAST

Accession

Primary (citable) accession number: P53037

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)