P53036 (SAP4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SIT4-associating protein SAP4

Gene names

Name:	SAP4
Ordered Locus Names:	YGL229C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	818 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Associates with the SIT4 phosphatase in a cell cycle dependent manner. May be directly or indirectly involved in SIT4-dependent functions in budding and in normal G1 cyclin expression By similarity.
Post-translational modification	Hyperphosphorylated in the absence of SIT4 By similarity.
Miscellaneous	Present with 279 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the SAPS family.

Ontologies

Keywords
Biological process	Cell cycle
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G1/S transition of mitotic cell cycle Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 818	818	SIT4-associating protein SAP4		PRO_0000097585

Sequences

	Sequence		Length	Mass (Da)
	P53036 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FB6E4FC06E4DD3FB	FASTA	818	94,729
	10 20 30 40 50 60 MSLWPFGETL SHSGIDSILE EYYLIFRSLE GNETSSTDDK KNEPSMESES EFGTESRDRS 70 80 90 100 110 120 DLNQSFIDRI LLETALLDEL NGAANDRLVD FICLGYFYDD RSQQVRHMDY LVDMLMAYLK 130 140 150 160 170 180 DIDRTGYRTP FLLENSFHQT GEYEDQDDED PMLYVNIISS IFCSKSAPIV EALVQNTPFL 190 200 210 220 230 240 SSLFEVFQFE NIEAENCPIL AVFLKINETL LFEQTSSYLE FFKSQPNIVD KFLYHIEVSP 250 260 270 280 290 300 LVEFLIKIML TDQVESPTNI IDFLYHQDLI PKCLNLLENS KYSPGIQNSS GELLKALISI 310 320 330 340 350 360 STNFKLDTLW IGPNRLTRQL ASPQYVDQLI NIILFQRGHA MGVAVSIIIE LIRKNNSDYD 370 380 390 400 410 420 EVDLLSTTIV DNPPSQRDPV YLGHLLYELT MHMEDFYALL IKLENDDDDD HDTASKALPS 430 440 450 460 470 480 VKHHLLENQL HESFRPLGFE RVKITELISE MLHCSNMGLM NSKRGEKIAR TRDKCRDTLD 490 500 510 520 530 540 QNSLEKAMKN LNINDNTITS NTLEDKCNNN DSNDSNDNQK QKKNIKKKFH DNELYSTFDT 550 560 570 580 590 600 SDDNIDDDDD MSFEIPYVSE TQNLKIRKNP TIGDLFKIKL HDLGFFPKFL QLFLRYPWNN 610 620 630 640 650 660 FWHNIVFDII QQIFNGRMDF SYNSFLVYSL FDFKKSTRFI PKPLYGSNQK LPVKDFHIIS 670 680 690 700 710 720 DFILQGHKDS FEFYEKEKTN LGYMGQLVLI AEEIAKYSKI YKTDLIAPDI YAFLQDEVWM 730 740 750 760 770 780 SYSSDILNET RTMCSIILGG GQFCAESDEN TNQDFLEKAD MSKPAHPSTM DENEIVHEED 790 800 810 VKLHDKVAEL IDELGQLTEL DIHDKIKDVI VDHHSDLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The SAPs, a new family of proteins, associate and function positively with the SIT4 phosphatase." Luke M.M., della Seta F., di Como C.J., Sugimoto H., Kobayashi R., Arndt K.T. Mol. Cell. Biol. 16:2744-2755(1996) [PubMed: 8649382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U50562 Genomic DNA. Translation: AAC49305.1. Z72751 Genomic DNA. Translation: CAA96947.1. Z72750 Genomic DNA. Translation: CAA96946.1. BK006941 Genomic DNA. Translation: DAA07889.1.
PIR	S64251.
RefSeq	NP_011285.1. NM_001181095.1.
3D structure databases
ProteinModelPortal	P53036.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1604N.
IntAct	P53036. 15 interactions.
MINT	MINT-386459.
STRING	P53036.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL229C; YGL229C; YGL229C.
GeneID	852622.
KEGG	sce:YGL229C.
NMPDR	fig|4932.3.peg.2388.
Organism-specific databases
CYGD	YGL229c.
SGD	S000003198. SAP4.
Phylogenomic databases
eggNOG	fuNOG08097.
GeneTree	EFGT00050000002207.
HOGENOM	HBG204381.
OrthoDB	EOG4SBJ67.
Gene expression databases
ArrayExpress	P53036.
Genevestigator	P53036.
GermOnline	YGL229C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007587. SAPS. [Graphical view]
PANTHER	PTHR12634. SAPS. 1 hit.
Pfam	PF04499. SAPS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971838.

Entry information

Entry name

SAP4_YEAST

Accession

Primary (citable) accession number: P53036
Secondary accession number(s): D6VVA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program