Reviewed, UniProtKB/Swiss-Prot P53035 (MIG2_YEAST)

Names and origin

Protein names

Recommended name:
    Regulatory protein MIG2

Gene names

Name:	MIG2
Synonyms:	MLZ1
Ordered Locus Names:	YGL209W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in glucose repression of the SUC2 gene.
Subcellular location	Nucleus Potential.
Miscellaneous	Present with 504 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the creA/MIG C2H2-type zinc-finger protein family. Contains 2 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription from RNA polymerase II promoter by glucose Inferred from genetic interaction. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Traceable author statement. Source: SGD
Molecular function	sequence-specific DNA binding Inferred from direct assay. Source: SGD specific transcriptional repressor activity Inferred from genetic interaction. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Regulatory protein MIG2		PRO_0000046882
Regions
	Zinc finger	17 – 39	23	C2H2-type 1
	Zinc finger	45 – 69	25	C2H2-type 2

Sequences

	Sequence		Length	Mass (Da)
	P53035-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BE25CBC21FB962C6	FASTA	382	42,048
	10 20 30 40 50 60 MPKKQTNFPV DNENRPFRCD TCHRGFHRLE HKKRHLRTHT GEKPHHCAFP GCGKSFSRSD 70 80 90 100 110 120 ELKRHMRTHT GQSQRRLKKA SVQKQEFLTV SGIPTIASGV MIHQPIPQVL PANMAINVQA 130 140 150 160 170 180 VNGGNIIHAP NAVHPMVIPI MAQPAPIHAS AASFQPATSP MPISTYTPVP SQSFTSFQSS 190 200 210 220 230 240 IGSIQSNSDV SSIFSNMNVR VNTPRSVPNS PNDGYLHQQH IPQQYQHQTA SPSVAKQQKT 250 260 270 280 290 300 FAHSLASALS TLQKRTPVSA PSTTIESPSS PSDSSHTSAS SSAISLPFSN APSQLAVAKE 310 320 330 340 350 360 LESVYLDSNR YTTKTRRERA KFEIPEEQEE DTNNSSSGSN EEEHESLDHE SSKSRKKLSG 370 380 VKLPPVRNLL KQIDVFNGPK RV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two zinc-finger-containing repressors are responsible for glucose repression of SUC2 expression." Lutfiyya L.L., Johnston M. Mol. Cell. Biol. 16:4790-4797(1996) [PubMed: 8756637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames." Kail M., Juettner E., Vaux D. Yeast 12:799-807(1996) [PubMed: 8813766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes." Feuermann M., Simeonava L., Souciet J.-L., Potier S. Yeast 13:475-477(1997) [PubMed: 9153757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U54564 Genomic DNA. Translation: AAB37570.1. U33754 Genomic DNA. Translation: AAC49496.1. Z72731 Genomic DNA. Translation: CAA96923.2. Z72731 Genomic DNA. Translation: CAA96924.1. Z72730 Genomic DNA. Translation: CAA96921.1. Z72732 Genomic DNA. Translation: CAA96925.1.
PIR	S71669.
RefSeq	NP_011306.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P53035. 7 interactions.
STRING	P53035.
Genome annotation databases
Ensembl	YGL209W; YGL209W; YGL209W; Saccharomyces cerevisiae. [Genome view]
GeneID	852663.
KEGG	sce:YGL209W.
NMPDR	fig|4932.3.peg.2409.
Organism-specific databases
CYGD	YGL209w.
SGD	S000003177. MIG2.
Phylogenomic databases
HOGENOM	P53035.
OrthoDB	EOG9TF23N
Gene expression databases
ArrayExpress	P53035.
Genevestigator	P53035.
GermOnline	YGL209W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 1 hit.
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 2 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 2 hits. PS50157. ZINC_FINGER_C2H2_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971951.

Entry information

Entry name

MIG2_YEAST

Accession

Primary (citable) accession number: P53035
Secondary accession number(s): O11853, O11854

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)