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Protein

Replication factor C subunit 2

Gene

RfC4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. Subunit 2 binds ATP.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi56 – 63ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB

GO - Biological processi

  • DNA damage checkpoint Source: FlyBase
  • DNA repair Source: FlyBase
  • DNA replication Source: UniProtKB
  • DNA replication checkpoint Source: FlyBase
  • DNA strand elongation involved in DNA replication Source: FlyBase
  • female meiosis chromosome segregation Source: FlyBase
  • lateral inhibition Source: FlyBase
  • leading strand elongation Source: FlyBase
  • mitotic cell cycle Source: FlyBase
  • mitotic chromosome condensation Source: FlyBase
  • sister chromatid cohesion Source: FlyBase

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-110312. Translesion synthesis by REV1.
R-DME-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-DME-110320. Translesion Synthesis by POLH.
R-DME-174411. Polymerase switching on the C-strand of the telomere.
R-DME-176187. Activation of ATR in response to replication stress.
R-DME-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-DME-5655862. Translesion synthesis by POLK.
R-DME-5656121. Translesion synthesis by POLI.
R-DME-5656169. Termination of translesion DNA synthesis.
R-DME-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-DME-6782135. Dual incision in TC-NER.
R-DME-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DME-69091. Polymerase switching.

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 2
Alternative name(s):
Activator 1 40 kDa subunit
Short name:
A1 40 kDa subunit
Activator 1 subunit 2
Replication factor C 40 kDa subunit
Short name:
RF-C 40 kDa subunit
Short name:
RFC40
Replication factor C subunit 4
Short name:
DmRfc4
Gene namesi
Name:RfC4
Synonyms:RfC40
ORF Names:CG14999
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0260985. RfC4.

Subcellular locationi

GO - Cellular componenti

  • DNA replication factor C complex Source: FlyBase
  • Elg1 RFC-like complex Source: FlyBase
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects in mitotic chromosome cohesion and condensation due to aberrant checkpoint control in response to DNA replication inhibition or damage to chromosomes; premature chromosome condensation and precocious sister chromatid separation figures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217691 – 331Replication factor C subunit 2Add BLAST331

Proteomic databases

PaxDbiP53034.
PRIDEiP53034.

Expressioni

Developmental stagei

Expressed in early embryos.1 Publication

Gene expression databases

BgeeiFBgn0260985.
GenevisibleiP53034. DM.

Interactioni

Subunit structurei

Heteropentamer of subunits of 140/145, 40, 38, 37, and 36.5 kDa that forms a complex with PCNA in the presence of ATP.By similarity

Protein-protein interaction databases

BioGridi63972. 14 interactors.
DIPiDIP-22077N.
IntActiP53034. 3 interactors.
MINTiMINT-925288.
STRINGi7227.FBpp0073120.

Structurei

3D structure databases

ProteinModelPortaliP53034.
SMRiP53034.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the activator 1 small subunits family.Curated

Phylogenomic databases

eggNOGiKOG0991. Eukaryota.
COG0470. LUCA.
GeneTreeiENSGT00550000075050.
InParanoidiP53034.
KOiK10755.
OMAiQSTWSGF.
OrthoDBiEOG091G0CKT.
PhylomeDBiP53034.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P53034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEEPEKTAD DKRSHLPWIE KYRPVKFKEI VGNEDTVARL SVFATQGNAP
60 70 80 90 100
NIIIAGPPGV GKTTTIQCLA RILLGDSYKE AVLELNASNE RGIDVVRNKI
110 120 130 140 150
KMFAQQKVTL PRGRHKIVIL DEADSMTEGA QQALRRTMEI YSSTTRFALA
160 170 180 190 200
CNTSEKIIEP IQSRCAMLRF TKLSDAQVLA KLIEVAKWEK LNYTEDGLEA
210 220 230 240 250
IVFTAQGDMR QGLNNLQSTA QGFGDITAEN VFKVCDEPHP KLLEEMIHHC
260 270 280 290 300
AANDIHKAYK ILAKLWKLGY SPEDIIANIF RVCKRINIDE HLKLDFIREI
310 320 330
GITHMKIIDG INSLLQLTAL LAKLCIAAEK H
Length:331
Mass (Da):37,173
Last modified:October 1, 1996 - v1
Checksum:i0ACE1C6DCBA8AF8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15967 Genomic DNA. Translation: AAB60241.1.
AE014296 Genomic DNA. Translation: AAF47843.1.
AY094829 mRNA. Translation: AAM11182.1.
PIRiS55020.
RefSeqiNP_523915.1. NM_079191.3.
UniGeneiDm.839.

Genome annotation databases

EnsemblMetazoaiFBtr0073264; FBpp0073120; FBgn0260985.
GeneIDi38492.
KEGGidme:Dmel_CG14999.

Similar proteinsi

Entry informationi

Entry nameiRFC2_DROME
AccessioniPrimary (citable) accession number: P53034
Secondary accession number(s): Q9VZH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: August 30, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families