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Protein

Replication factor C subunit 2

Gene

RfC4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. Subunit 2 binds ATP.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi56 – 63ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB

GO - Biological processi

  • DNA damage checkpoint Source: FlyBase
  • DNA repair Source: FlyBase
  • DNA replication Source: UniProtKB
  • DNA replication checkpoint Source: FlyBase
  • DNA strand elongation involved in DNA replication Source: FlyBase
  • female meiosis chromosome segregation Source: FlyBase
  • lateral inhibition Source: FlyBase
  • leading strand elongation Source: FlyBase
  • mitotic chromosome condensation Source: FlyBase
  • mitotic nuclear division Source: FlyBase
  • neurogenesis Source: FlyBase
  • sister chromatid cohesion Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-110312. Translesion synthesis by REV1.
R-DME-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-DME-110320. Translesion Synthesis by POLH.
R-DME-174411. Polymerase switching on the C-strand of the telomere.
R-DME-176187. Activation of ATR in response to replication stress.
R-DME-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-DME-5655862. Translesion synthesis by POLK.
R-DME-5656121. Translesion synthesis by POLI.
R-DME-5656169. Termination of translesion DNA synthesis.
R-DME-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-DME-6782135. Dual incision in TC-NER.
R-DME-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DME-69091. Polymerase switching.

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 2
Alternative name(s):
Activator 1 40 kDa subunit
Short name:
A1 40 kDa subunit
Activator 1 subunit 2
Replication factor C 40 kDa subunit
Short name:
RF-C 40 kDa subunit
Short name:
RFC40
Replication factor C subunit 4
Short name:
DmRfc4
Gene namesi
Name:RfC4
Synonyms:RfC40
ORF Names:CG14999
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0260985. RfC4.

Subcellular locationi

GO - Cellular componenti

  • DNA replication factor C complex Source: FlyBase
  • microtubule associated complex Source: FlyBase
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects in mitotic chromosome cohesion and condensation due to aberrant checkpoint control in response to DNA replication inhibition or damage to chromosomes; premature chromosome condensation and precocious sister chromatid separation figures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217691 – 331Replication factor C subunit 2Add BLAST331

Proteomic databases

PaxDbiP53034.
PRIDEiP53034.

Expressioni

Developmental stagei

Expressed in early embryos.1 Publication

Gene expression databases

BgeeiFBgn0260985.
GenevisibleiP53034. DM.

Interactioni

Subunit structurei

Heteropentamer of subunits of 140/145, 40, 38, 37, and 36.5 kDa that forms a complex with PCNA in the presence of ATP.By similarity

Protein-protein interaction databases

BioGridi63972. 14 interactors.
DIPiDIP-22077N.
IntActiP53034. 3 interactors.
MINTiMINT-925288.
STRINGi7227.FBpp0073120.

Structurei

3D structure databases

ProteinModelPortaliP53034.
SMRiP53034.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the activator 1 small subunits family.Curated

Phylogenomic databases

eggNOGiKOG0991. Eukaryota.
COG0470. LUCA.
GeneTreeiENSGT00550000075050.
InParanoidiP53034.
KOiK10755.
OMAiHHCAAND.
OrthoDBiEOG091G0CKT.
PhylomeDBiP53034.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P53034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEEPEKTAD DKRSHLPWIE KYRPVKFKEI VGNEDTVARL SVFATQGNAP
60 70 80 90 100
NIIIAGPPGV GKTTTIQCLA RILLGDSYKE AVLELNASNE RGIDVVRNKI
110 120 130 140 150
KMFAQQKVTL PRGRHKIVIL DEADSMTEGA QQALRRTMEI YSSTTRFALA
160 170 180 190 200
CNTSEKIIEP IQSRCAMLRF TKLSDAQVLA KLIEVAKWEK LNYTEDGLEA
210 220 230 240 250
IVFTAQGDMR QGLNNLQSTA QGFGDITAEN VFKVCDEPHP KLLEEMIHHC
260 270 280 290 300
AANDIHKAYK ILAKLWKLGY SPEDIIANIF RVCKRINIDE HLKLDFIREI
310 320 330
GITHMKIIDG INSLLQLTAL LAKLCIAAEK H
Length:331
Mass (Da):37,173
Last modified:October 1, 1996 - v1
Checksum:i0ACE1C6DCBA8AF8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15967 Genomic DNA. Translation: AAB60241.1.
AE014296 Genomic DNA. Translation: AAF47843.1.
AY094829 mRNA. Translation: AAM11182.1.
PIRiS55020.
RefSeqiNP_523915.1. NM_079191.3.
UniGeneiDm.839.

Genome annotation databases

EnsemblMetazoaiFBtr0073264; FBpp0073120; FBgn0260985.
GeneIDi38492.
KEGGidme:Dmel_CG14999.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15967 Genomic DNA. Translation: AAB60241.1.
AE014296 Genomic DNA. Translation: AAF47843.1.
AY094829 mRNA. Translation: AAM11182.1.
PIRiS55020.
RefSeqiNP_523915.1. NM_079191.3.
UniGeneiDm.839.

3D structure databases

ProteinModelPortaliP53034.
SMRiP53034.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63972. 14 interactors.
DIPiDIP-22077N.
IntActiP53034. 3 interactors.
MINTiMINT-925288.
STRINGi7227.FBpp0073120.

Proteomic databases

PaxDbiP53034.
PRIDEiP53034.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073264; FBpp0073120; FBgn0260985.
GeneIDi38492.
KEGGidme:Dmel_CG14999.

Organism-specific databases

CTDi5984.
FlyBaseiFBgn0260985. RfC4.

Phylogenomic databases

eggNOGiKOG0991. Eukaryota.
COG0470. LUCA.
GeneTreeiENSGT00550000075050.
InParanoidiP53034.
KOiK10755.
OMAiHHCAAND.
OrthoDBiEOG091G0CKT.
PhylomeDBiP53034.

Enzyme and pathway databases

ReactomeiR-DME-110312. Translesion synthesis by REV1.
R-DME-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-DME-110320. Translesion Synthesis by POLH.
R-DME-174411. Polymerase switching on the C-strand of the telomere.
R-DME-176187. Activation of ATR in response to replication stress.
R-DME-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-DME-5655862. Translesion synthesis by POLK.
R-DME-5656121. Translesion synthesis by POLI.
R-DME-5656169. Termination of translesion DNA synthesis.
R-DME-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-DME-6782135. Dual incision in TC-NER.
R-DME-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DME-69091. Polymerase switching.

Miscellaneous databases

GenomeRNAii38492.
PROiP53034.

Gene expression databases

BgeeiFBgn0260985.
GenevisibleiP53034. DM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFC2_DROME
AccessioniPrimary (citable) accession number: P53034
Secondary accession number(s): Q9VZH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.