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Protein

Elongation factor 1-alpha

Gene

eft-3

more
Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTPBy similarity
Nucleotide bindingi91 – 955GTPBy similarity
Nucleotide bindingi153 – 1564GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: WormBase
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: UniProtKB-KW
  • translation factor activity, RNA binding Source: WormBase

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: WormBase
  • growth Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-156842. Eukaryotic Translation Elongation.
R-CEL-3371511. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alpha
Short name:
EF-1-alpha
Gene namesi
Name:eft-3
ORF Names:F31E3.5
AND
Name:eft-4
ORF Names:R03G5.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componentsi: Chromosome III, Chromosome X

Organism-specific databases

WormBaseiF31E3.5; CE01270; WBGene00001168; eft-3.
R03G5.1a; CE01270; WBGene00001169; eft-4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Elongation factor 1-alphaPRO_0000090918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei301 – 30115-glutamyl glycerylphosphorylethanolamineBy similarity
Modified residuei374 – 37415-glutamyl glycerylphosphorylethanolamineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP53013.
PaxDbiP53013.
PRIDEiP53013.

PTM databases

iPTMnetiP53013.

Expressioni

Gene expression databases

ExpressionAtlasiP53013. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi41189. 13 interactions.
DIPiDIP-26595N.
IntActiP53013. 5 interactions.
MINTiMINT-210627.
STRINGi6239.R03G5.1a.3.

Structurei

3D structure databases

ProteinModelPortaliP53013.
SMRiP53013. Positions 4-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 242238tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 218G1PROSITE-ProRule annotation
Regioni70 – 745G2PROSITE-ProRule annotation
Regioni91 – 944G3PROSITE-ProRule annotation
Regioni153 – 1564G4PROSITE-ProRule annotation
Regioni194 – 1963G5PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0052. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00670000097815.
HOGENOMiHOG000229291.
InParanoidiP53013.
KOiK03231.
OMAiASEKMPW.
OrthoDBiEOG7NKKK3.
PhylomeDBiP53013.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKEKVHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAQEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDIALWKF ETAKYYITII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLVVACGT GEFEAGISKN GQTREHALLA QTLGVKQLIV
160 170 180 190 200
ACNKMDSTEP PFSEARFTEI TNEVSGFIKK IGYNPKAVPF VPISGFNGDN
210 220 230 240 250
MLEVSSNMPW FKGWAVERKE GNASGKTLLE ALDSIIPPQR PTDRPLRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG IIKPGMVVTF APQNVTTEVK SVEMHHESLP
310 320 330 340 350
EAVPGDNVGF NVKNVSVKDI RRGSVCSDSK QDPAKEARTF HAQVIIMNHP
360 370 380 390 400
GQISNGYTPV LDCHTAHIAC KFNELKEKVD RRTGKKVEDF PKFLKSGDAG
410 420 430 440 450
IVELIPTKPL CVESFTDYAP LGRFAVRDMR QTVAVGVIKS VEKSDGSSGK
460
VTKSAQKAAP KKK
Length:463
Mass (Da):50,668
Last modified:October 1, 1996 - v1
Checksum:i12544AF1F17E15B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080948 Genomic DNA. Translation: CCD68024.1.
FO080392 Genomic DNA. Translation: CCD63407.1.
PIRiT16218.
RefSeqiNP_498520.1. NM_066119.6.
NP_509323.1. NM_076922.4.
UniGeneiCel.22702.
Cel.22703.

Genome annotation databases

EnsemblMetazoaiF31E3.5.1; F31E3.5.1; WBGene00001168.
F31E3.5.2; F31E3.5.2; WBGene00001168.
R03G5.1a.1; R03G5.1a.1; WBGene00001169.
R03G5.1a.2; R03G5.1a.2; WBGene00001169.
R03G5.1a.3; R03G5.1a.3; WBGene00001169.
R03G5.1a.4; R03G5.1a.4; WBGene00001169.
GeneIDi175975.
181044.
KEGGicel:CELE_F31E3.5.
cel:CELE_R03G5.1.
UCSCiF31E3.5.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080948 Genomic DNA. Translation: CCD68024.1.
FO080392 Genomic DNA. Translation: CCD63407.1.
PIRiT16218.
RefSeqiNP_498520.1. NM_066119.6.
NP_509323.1. NM_076922.4.
UniGeneiCel.22702.
Cel.22703.

3D structure databases

ProteinModelPortaliP53013.
SMRiP53013. Positions 4-443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41189. 13 interactions.
DIPiDIP-26595N.
IntActiP53013. 5 interactions.
MINTiMINT-210627.
STRINGi6239.R03G5.1a.3.

PTM databases

iPTMnetiP53013.

Proteomic databases

EPDiP53013.
PaxDbiP53013.
PRIDEiP53013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF31E3.5.1; F31E3.5.1; WBGene00001168.
F31E3.5.2; F31E3.5.2; WBGene00001168.
R03G5.1a.1; R03G5.1a.1; WBGene00001169.
R03G5.1a.2; R03G5.1a.2; WBGene00001169.
R03G5.1a.3; R03G5.1a.3; WBGene00001169.
R03G5.1a.4; R03G5.1a.4; WBGene00001169.
GeneIDi175975.
181044.
KEGGicel:CELE_F31E3.5.
cel:CELE_R03G5.1.
UCSCiF31E3.5.2. c. elegans.

Organism-specific databases

CTDi175975.
181044.
WormBaseiF31E3.5; CE01270; WBGene00001168; eft-3.
R03G5.1a; CE01270; WBGene00001169; eft-4.

Phylogenomic databases

eggNOGiKOG0052. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00670000097815.
HOGENOMiHOG000229291.
InParanoidiP53013.
KOiK03231.
OMAiASEKMPW.
OrthoDBiEOG7NKKK3.
PhylomeDBiP53013.

Enzyme and pathway databases

ReactomeiR-CEL-156842. Eukaryotic Translation Elongation.
R-CEL-3371511. HSF1 activation.

Miscellaneous databases

NextBioi890570.
PROiP53013.

Gene expression databases

ExpressionAtlasiP53013. baseline and differential.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (EFT-3 AND EFT-4).
    Strain: Bristol N2.

Entry informationi

Entry nameiEF1A_CAEEL
AccessioniPrimary (citable) accession number: P53013
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.