P53007 (TXTP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tricarboxylate transport protein, mitochondrial

Alternative name(s):
Citrate transport protein
Short name=CTP
Solute carrier family 25 member 1
Tricarboxylate carrier protein

Gene names

Name:	SLC25A1
Synonyms:	SLC20A3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in citrate-H+/malate exchange. Important for the bioenergetics of hepatic cells as it provides a carbon source for fatty acid and sterol biosyntheses, and NAD+ for the glycolytic pathway.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification] Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transit peptide Transmembrane Transmembrane helix
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Traceable author statement. Source: Reactome long-chain fatty-acyl-CoA biosynthetic process Traceable author statement. Source: Reactome mitochondrial citrate transport Traceable author statement. Source: UniProtKB small molecule metabolic process Traceable author statement. Source: Reactome triglyceride biosynthetic process Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Traceable author statement. Source: UniProtKB
Molecular_function	citrate transmembrane transporter activity Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 13	13	Mitochondrion By similarity
	Chain	14 – 311	298	Tricarboxylate transport protein, mitochondrial		PRO_0000019262
Regions
	Transmembrane	29 – 46	18	Helical; Name=1; Potential
	Transmembrane	86 – 105	20	Helical; Name=2; Potential
	Transmembrane	129 – 143	15	Helical; Name=3; Potential
	Transmembrane	183 – 202	20	Helical; Name=4; Potential
	Transmembrane	224 – 241	18	Helical; Name=5; Potential
	Transmembrane	278 – 297	20	Helical; Name=6; Potential
	Repeat	23 – 111	89	Solcar 1
	Repeat	122 – 208	87	Solcar 2
	Repeat	218 – 303	86	Solcar 3
Amino acid modifications
	Modified residue	97	1	N6-acetyllysine Ref.5
Experimental info
	Sequence conflict	26	1	G → E in AAB08515. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53007 [UniParc]. Last modified February 11, 2002. Version 2. Checksum: F1341629924953D6	FASTA	311	34,013
	10 20 30 40 50 60 MPAPRAPRAL AAAAPASGKA KLTHPGKAIL AGGLAGGIEI CITFPTEYVK TQLQLDERSH 70 80 90 100 110 120 PPRYRGIGDC VRQTVRSHGV LGLYRGLSSL LYGSIPKAAV RFGMFEFLSN HMRDAQGRLD 130 140 150 160 170 180 STRGLLCGLG AGVAEAVVVV CPMETIKVKF IHDQTSPNPK YRGFFHGVRE IVREQGLKGT 190 200 210 220 230 240 YQGLTATVLK QGSNQAIRFF VMTSLRNWYR GDNPNKPMNP LITGVFGAIA GAASVFGNTP 250 260 270 280 290 300 LDVIKTRMQG LEAHKYRNTW DCGLQILKKE GLKAFYKGTV PRLGRVCLDV AIVFVIYDEV 310 VKLLNKVWKT D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Localization of the human mitochondrial citrate transporter protein gene to chromosome 22q11 in the DiGeorge syndrome critical region." Heisterkamp N., Mulder M.P., Langeveld A., ten Hoeve J., Wang Z., Roe B., Groffen J. Genomics 29:451-456(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning, genomic organization, and chromosomal localization of human citrate transport protein to the DiGeorge/velocardiofacial syndrome minimal critical region." Goldmuntz E., Wang Z., Roe B.A., Budarf M.L. Genomics 33:271-276(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-97, MASS SPECTROMETRY.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25147 mRNA. Translation: AAB08515.1. L76134 Genomic DNA. Translation: AAL40091.1. L75823 mRNA. Translation: AAL40090.1. AK292313 mRNA. Translation: BAF85002.1. BC004980 mRNA. Translation: AAH04980.1. BC008061 mRNA. Translation: AAH08061.1.
IPI	IPI00294159.
PIR	G01789.
RefSeq	NP_005975.1. NM_005984.3.
UniGene	Hs.111024.
3D structure databases
ProteinModelPortal	P53007.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53007. 6 interactions.
MINT	MINT-1153318.
STRING	9606.ENSP00000215882.
Protein family/group databases
TCDB	2.A.29.7.2. mitochondrial carrier (MC) family.
PTM databases
PhosphoSite	P53007.
Polymorphism databases
DMDM	20141931.
Proteomic databases
PaxDb	P53007.
PeptideAtlas	P53007.
PRIDE	P53007.
Protocols and materials databases
DNASU	6576.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000215882; ENSP00000215882; ENSG00000100075. ENST00000598008; ENSP00000472050; ENSG00000268928.
GeneID	6576.
KEGG	hsa:6576.
UCSC	uc002zoy.3. human.
Organism-specific databases
CTD	6576.
GeneCards	GC22M019163.
H-InvDB	HIX0040279.
HGNC	HGNC:10979. SLC25A1.
HPA	HPA030183.
MIM	190315. gene.
neXtProt	NX_P53007.
PharmGKB	PA35855.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG306627.
HOVERGEN	HBG103009.
InParanoid	P53007.
KO	K15100.
OMA	FAVTPME.
OrthoDB	EOG44BB2X.
PhylomeDB	P53007.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	P53007.
Bgee	P53007.
CleanEx	HS_SLC25A1.
Genevestigator	P53007.
GermOnline	ENSG00000100075. Homo sapiens.
Family and domain databases
Gene3D	1.50.40.10. 1 hit.
InterPro	IPR002067. Mit_carrier. IPR018108. Mitochondrial_sb/sol_carrier. IPR023395. Mt_carrier_dom. [Graphical view]
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00926. MITOCARRIER.
SUPFAM	SSF103506. Mitoch_carrier. 1 hit.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC25A1. human.
GenomeRNAi	6576.
NextBio	25587.
SOURCE	Search...

Entry information

Entry name

TXTP_HUMAN

Accession

Primary (citable) accession number: P53007
Secondary accession number(s): A8K8E8, Q9BSK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 11, 2002
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.