Reviewed, UniProtKB/Swiss-Prot P53000 (PROA_CAMJE)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Cj0558c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Gamma-glutamyl phosphate reductase		PRO_0000189710
Experimental info
	Sequence conflict	57	1	A → K in AAA23030. Ref.1
	Sequence conflict	100	1	S → N in AAA23030. Ref.1
	Sequence conflict	104	1	M → I in AAA23030. Ref.1
	Sequence conflict	283	1	A → T in AAA23030. Ref.1
	Sequence conflict	293	1	K → E in AAA23030. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53000-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: EB27A90652906668	FASTA	410	46,000
	10 20 30 40 50 60 MRNLLENIKK NSQKLLNLTP KDKEKIILKL AQILRENFKI ILEANKKDMA NFTKSGAMKD 70 80 90 100 110 120 RLLLDEKRIL ALCEGLEKIA YIEDPIGKIS KGWKNYAGLS IQKMSIPLGL ICVIYEARPS 130 140 150 160 170 180 LSAEIAALMI KSSNACVFKG GSEAKFTNEA IFTLVNKVLK EFDLQDCFAM FTQRDEILQI 190 200 210 220 230 240 LAFDDLIDVI IPRGSSNMIQ EIANNTKIPL IKQNKGLCHA FVDQSANLDM ALKIILNAKC 250 260 270 280 290 300 QRVSVCNALE TLLIHEKIAK NFISLLIPEF EKFKVKIHAH ENALAYFNNS NLKIFKANEN 310 320 330 340 350 360 TFDTEWLDFA LSVKLVKDCD EAIEHINKHS SLHSETIISN DASNIAKFQR LINSSCIYAN 370 380 390 400 410 ASTRFSDGGE FGFGGEVGIS TSKLHARGPM GIEDICTYKY IINGEGQIRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the gamma-glutamyl phosphate reductase gene of Campylobacter jejuni." Louie H., Chan V.L. Mol. Gen. Genet. 240:29-35(1993) [PubMed: 8341262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M74579 Genomic DNA. Translation: AAA23030.1. AL111168 Genomic DNA. Translation: CAL34704.1.
PIR	F81402. S35213.
3D structure databases
HSSP	HSSP built from PDB template 1O20 based on UniProtKB Q9WYC9.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus Cj0558c in contig AL111168_GR.
KEGG	cje:Cj0558c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P53000.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0558C-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CAMJE

Accession

Primary (citable) accession number: P53000
Secondary accession number(s): Q0PAV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)