Reviewed,
UniProtKB/Swiss-Prot P52996 (PANB_BACSU)
Last modified
November 3, 2009.
Version 65.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Alternative name(s): Ketopantoate hydroxymethyltransferase Short name=KPHMT | ||||
| Gene names |
| ||||
| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 1423 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 277 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. |
| Catalytic activity | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156 |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. |
| Pathway | Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156 |
| Subunit structure | Homodecamer; pentamer of dimers By similarity. |
| Subcellular location | Cytoplasm Potential. |
| Sequence similarities | Belongs to the panB family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Pantothenate biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | Magnesium Metal-binding |
| Molecular function | Methyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 277 | 277 | 3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156 | PRO_0000184816 | |||||
Regions | |||||||||
| Region | 43 – 44 | 2 | Alpha-ketoisovalerate binding By similarity | ||||||
Sites | |||||||||
| Active site | 181 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 43 | 1 | Magnesium By similarity | ||||||
| Metal binding | 82 | 1 | Magnesium By similarity | ||||||
| Metal binding | 114 | 1 | Magnesium By similarity | ||||||
| Binding site | 82 | 1 | Alpha-ketoisovalerate By similarity | ||||||
| Binding site | 112 | 1 | Alpha-ketoisovalerate By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome." Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P. Microbiology 142:2005-2016(1996) [PubMed: 8760912] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg. |
| [2] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
Cross-references
Sequence databases | |
|---|---|
| L47709 Genomic DNA. Translation: AAB38448.1. AL009126 Genomic DNA. Translation: CAB14159.1. | |
| PIR | G69671. |
| RefSeq | NP_390124.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1OY0 based on UniProtKB Q10505. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 939032. |
| GenomeReviews | Gene locus BSU22430 in contig AL009126_GR. |
| KEGG | bsu:BSU22430. |
| NMPDR | fig|224308.1.peg.2247. |
Organism-specific databases | |
| SubtiList | BG11519. panB. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P52996. |
| OMA | YATPEQT. |
Enzyme and pathway databases | |
| BioCyc | BSUB224308:BSU2242-MON. |
| BRENDA | 2.1.2.11. 150. |
Family and domain databases | |
| HAMAP | MF_00156. [Tree] |
| InterPro | IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view] |
| Gene3D | G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. |
| PANTHER | PTHR20881. Pantoate_transf. 1 hit. |
| Pfam | PF02548. Pantoate_transf. 1 hit. [Graphical view] |
| PIRSF | PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit. |
| TIGRFAMs | TIGR00222. panB. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | PANB_BACSU | ||||||||
| Accession | Primary (citable) accession number: P52996 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
