Reviewed, UniProtKB/Swiss-Prot P52986 (DHOM_SYNY3)

Names and origin

Protein names

Recommended name:
    Homoserine dehydrogenase
      Short name=HDH
    EC=1.1.1.3

Gene names

Name:	hom
Ordered Locus Names:	sll0455

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Sequence similarities	Belongs to the homoserine dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Isoleucine biosynthesis Methionine biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro homoserine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Homoserine dehydrogenase		PRO_0000066704
Regions
	Domain	355 – 428	74	ACT
	Nucleotide binding	8 – 15	8	NADP By similarity
Sites
	Active site	202	1	Proton donor Potential
	Binding site	102	1	NADP By similarity
	Binding site	187	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52986-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4A7A034A7D2766B5	FASTA	433	45,486
	10 20 30 40 50 60 MTVKIGLLGL GTVGSGTVEI LQDPQGRSPL LKAIEVKAVG VRSLDKPRQV NLPPEVLTTD 70 80 90 100 110 120 LEAIVTDPDI AIVVELMGGL EPARSLILQA IAHKKHIVTA NKAVIARYGP EIYEAANQHG 130 140 150 160 170 180 VYVLLEAAVG GGIPIIKPLK QSLGGNRIQS IVGILNGTTN YILSRMTSEG ADFDEVLTAA 190 200 210 220 230 240 QQLGYAEADP SADVDGLDAA DKIAILASLG FGGRVKREDV ACEGIRSVSA VDIAYADRLG 250 260 270 280 290 300 FVIKLLAIAD GNAGEDSEAL QLRVHPTLIA KDHPLASVNG VYNGVLVTGD PLGQVMFYGR 310 320 330 340 350 360 GAGAGPTASA VVSDVINIVG IITSSDENPA LDPLLSCTHQ HYCQVSPIED LVTRFYCRFL 370 380 390 400 410 420 CADVPGVIGH LGMGFGNHGV SLESLVQIGF TDGCAEIVVV THDVREGDYR AALEEISQLE 430 AVKEIPSVIR VLS

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10325.1.
PIR	S74407.
RefSeq	NP_442255.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P52986. 2 interactions.
STRING	P52986.
Genome annotation databases
GeneID	952451.
GenomeReviews	Gene locus sll0455 in contig BA000022_GR.
KEGG	syn:sll0455.
NMPDR	fig|1148.1.peg.2356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0460.
HOGENOM	HBG527379.
OMA	LGIVNGT.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0455-MONOMER.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR005106. Asp/hSer_DH_NAD-bd. IPR016204. Homoserine_dehydrogenase. IPR001342. Homoserine_dehydrogenase_cat. IPR019811. Homoserine_dehydrogenase_CS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01842. ACT. 1 hit. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF000098. Homoser_dehydrog. 1 hit.
PROSITE	PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHOM_SYNY3

Accession

Primary (citable) accession number: P52986

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)