Reviewed, UniProtKB/Swiss-Prot P52985 (DHOM_LACLC)

Names and origin

Protein names

Recommended name:
    Homoserine dehydrogenase
      Short name=HDH
    EC=1.1.1.3

Gene names

Name:

hom

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Sequence similarities	Belongs to the homoserine dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Isoleucine biosynthesis Methionine biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro homoserine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Homoserine dehydrogenase		PRO_0000066697
Regions
	Domain	350 – 423	74	ACT
	Nucleotide binding	8 – 15	8	NADP By similarity
Sites
	Active site	206	1	Proton donor Potential
	Binding site	106	1	NADP By similarity
	Binding site	191	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52985-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B178CF30D62A6349	FASTA	428	46,629
	10 20 30 40 50 60 MAVNIAILGF GTVGTGLPTL LSENKEKLAK ILDEEIVISK VLMRDNKAIE KARSQGFNYD 70 80 90 100 110 120 FVLNLDDILA DSEISIVVEL MGRIEPAKTY ITQAIEAGKN VVTANKDLLA VHGVELRSLA 130 140 150 160 170 180 QKHHVALYYE AAVAGGIPIL RTLANSFSSD KITHLLGILN GTSNFMMTKM SEEGWTYDES 190 200 210 220 230 240 LAKAQELGYA ESDPTNDVDG IDASYKLAIL SEFAFGMTLA PDDIAKSGLR SIQKTDVEIA 250 260 270 280 290 300 QQFGYVLKLT GEINEVDSGI FAEVSPTFLP KSHPLASVNG VMNAVFIESE GIGDSVFYGA 310 320 330 340 350 360 GAGQKPTATS VLADIVRIVK RVKDGTIGKS FNEYARSTSL ANPHDIENKY YFSVETPDST 370 380 390 400 410 420 GQLLLLVELF TSENVSFEQV LQQKGNGKRA VVVIISHKIN RVQLSAIQDK LNQEKDFKLL NRFKVLGD

References

[1]	"Cloning and transcriptional analysis of two threonine biosynthetic genes from Lactococcus lactis MG1614." Madsen S.M., Albrechtsen B., Hansen E.B., Israelsen H. J. Bacteriol. 178:3689-3694(1996) [PubMed: 8682767] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MG1614.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X96988 Genomic DNA. Translation: CAA65713.1.
PIR	JC6049.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.3. 289716.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR005106. Asp/hSer_DH_NAD-bd. IPR016204. Homoserine_dehydrogenase. IPR001342. Homoserine_dehydrogenase_cat. IPR019811. Homoserine_dehydrogenase_CS. [Graphical view]
Pfam	PF01842. ACT. 1 hit. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF000098. Homoser_dehydrog. 1 hit.
PROSITE	PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHOM_LACLC

Accession

Primary (citable) accession number: P52985

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)