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P52981 (GLGB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:sll0158
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length770 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

trxAP522311EBI-862111,EBI-862916

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7707701,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188758

Sites

Active site4331Nucleophile By similarity
Active site4861Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P52981 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: A435AFCA7703FA8A

FASTA77089,528
        10         20         30         40         50         60 
MTYTINADQV HQIVHNLHHD PFEVLGCHPL GDHGKVNQWV IRAYLPTAEA VTVLLPTDRR 

        70         80         90        100        110        120 
EVIMTTVHHP NFFECVLELE EPKNYQLRIT ENGHERVIYD PYGFKTPKLT DFDLHVFGEG 

       130        140        150        160        170        180 
NHHRIYEKLG AHLMTVDGVK GVYFAVWAPN ARNVSILGDF NNWDGRLHQM RKRNNMVWEL 

       190        200        210        220        230        240 
FIPELGVGTS YKYEIKNWEG HIYEKTDPYG FYQEVRPKTA SIVADLDGYQ WHDEDWLEAR 

       250        260        270        280        290        300 
RTSDPLSKPV SVYELHLGSW LHTAYDEPVK TLHGEGVPVE VSEWNTGARF LTYYELVDKL 

       310        320        330        340        350        360 
IPYVKELGYT HIELLPIAEH PFDGSWGYQV TGYYAPTSRF GSPEDFMYFV DQCHLNGIGV 

       370        380        390        400        410        420 
IIDWVPGHFP KDGHGLAFFD GTHLYEHGDP RKGEHKEWGT LIFNYGRNEV RNFLVANALF 

       430        440        450        460        470        480 
WFDKYHIDGM RVDAVASMLY LDYCREEGEW VANEYGGREN LEAADFLRQV NSVVYSYFPG 

       490        500        510        520        530        540 
ILSIAEESTS WPMVSWPTYV GGLGFNLKWN MGWMHDMLDY FSMDPWFRQF HQNSITFSMW 

       550        560        570        580        590        600 
YNHSENYMLA LSHDEVVHGK SNMLGKMPGD EWQKYANVRA LFTYMFTHPG KKTMFMSMEF 

       610        620        630        640        650        660 
GQWSEWNVWG DLEWHLLNFP PHQQLKQFFT ELNHLYKNEP ALYSNDFDES GFQWIDCSDN 

       670        680        690        700        710        720 
RHSVVSFIRR AKNSAEFVVT ICNFTPQPHS HYRVGVPVPG FYTELFNSDA RQYGGSNMGN 

       730        740        750        760        770 
LGGKWTEEWS FHEQPYSLDL CLPPLSVLVL KLSQNAEENT VPAEEASNIA 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27184 / PCC 6803 / N-1.
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA10073.1.
PIRS76095.
RefSeqNP_442003.1. NC_000911.1.
YP_005652063.1. NC_017277.1.
YP_007451883.1. NC_020286.1.

3D structure databases

ProteinModelPortalP52981.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP52981. 1 interaction.
STRING1148.sll0158.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbP52981.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA10073; BAA10073; BAA10073.
GeneID952758.
KEGGsyn:sll0158.
syy:SYNGTS_2110.
syz:MYO_121300.
PATRIC23841580. VBISynSp132158_2338.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.
PhylomeDBP52981.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 2 hits.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_SYNY3
AccessionPrimary (citable) accession number: P52981
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries