Reviewed, UniProtKB/Swiss-Prot P52981 (GLGB_SYNY3)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	sll0158

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	770 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P52231	1	EBI-862111,EBI-862916

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 770	770	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188758
Sites
	Active site	328	1	By similarity
	Active site	363	1	By similarity
	Active site	368	1	By similarity
	Active site	431	1	By similarity
	Active site	433	1	By similarity
	Active site	486	1	By similarity
	Active site	553	1	By similarity
	Active site	554	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52981-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A435AFCA7703FA8A	FASTA	770	89,528
	10 20 30 40 50 60 MTYTINADQV HQIVHNLHHD PFEVLGCHPL GDHGKVNQWV IRAYLPTAEA VTVLLPTDRR 70 80 90 100 110 120 EVIMTTVHHP NFFECVLELE EPKNYQLRIT ENGHERVIYD PYGFKTPKLT DFDLHVFGEG 130 140 150 160 170 180 NHHRIYEKLG AHLMTVDGVK GVYFAVWAPN ARNVSILGDF NNWDGRLHQM RKRNNMVWEL 190 200 210 220 230 240 FIPELGVGTS YKYEIKNWEG HIYEKTDPYG FYQEVRPKTA SIVADLDGYQ WHDEDWLEAR 250 260 270 280 290 300 RTSDPLSKPV SVYELHLGSW LHTAYDEPVK TLHGEGVPVE VSEWNTGARF LTYYELVDKL 310 320 330 340 350 360 IPYVKELGYT HIELLPIAEH PFDGSWGYQV TGYYAPTSRF GSPEDFMYFV DQCHLNGIGV 370 380 390 400 410 420 IIDWVPGHFP KDGHGLAFFD GTHLYEHGDP RKGEHKEWGT LIFNYGRNEV RNFLVANALF 430 440 450 460 470 480 WFDKYHIDGM RVDAVASMLY LDYCREEGEW VANEYGGREN LEAADFLRQV NSVVYSYFPG 490 500 510 520 530 540 ILSIAEESTS WPMVSWPTYV GGLGFNLKWN MGWMHDMLDY FSMDPWFRQF HQNSITFSMW 550 560 570 580 590 600 YNHSENYMLA LSHDEVVHGK SNMLGKMPGD EWQKYANVRA LFTYMFTHPG KKTMFMSMEF 610 620 630 640 650 660 GQWSEWNVWG DLEWHLLNFP PHQQLKQFFT ELNHLYKNEP ALYSNDFDES GFQWIDCSDN 670 680 690 700 710 720 RHSVVSFIRR AKNSAEFVVT ICNFTPQPHS HYRVGVPVPG FYTELFNSDA RQYGGSNMGN 730 740 750 760 770 LGGKWTEEWS FHEQPYSLDL CLPPLSVLVL KLSQNAEENT VPAEEASNIA

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10073.1.
PIR	S76095.
RefSeq	NP_442003.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52981. 1 interaction.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	952758.
GenomeReviews	Gene locus sll0158 in contig BA000022_GR.
KEGG	syn:sll0158.
NMPDR	fig|1148.1.peg.2104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P52981.
OMA	P52981. PKTASIV.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0158-MON.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 2 hits. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_SYNY3

Accession

Primary (citable) accession number: P52981

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)