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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Streptomyces aureofaciens
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei440 – 4401NucleophileBy similarity
Active sitei493 – 4931Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
OrganismiStreptomyces aureofaciens
Taxonomic identifieri1894 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7647641,4-alpha-glucan branching enzyme GlgBPRO_0000188746Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP52980.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

P52980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAARQPSPT VRDKAAPEPA APAAPKGARA PRARRAAPPH GVRPAPALAA
60 70 80 90 100
EERARLLEGR HHDPHAVLGA RTQRGGVAFR VLRPYAKAVT VVAKGLRTEL
110 120 130 140 150
VDEGDGLFSG LLPLTGVPDY RLLVTYDSDE IEVHDPYRFL PALGELDLHL
160 170 180 190 200
IGEGRHEELW TALGSQPMEH QGVAGTRFTV WAPNALGVRV TGDFSYWDAV
210 220 230 240 250
AYPMRSLGAS GVWELFLPGV AEGALYKYEI TRPDGGRTLR ADPMARYAEV
260 270 280 290 300
PPANASIVTA SRYEWQDAEW MARRGALAPH QAPMSVYELH LASWRPGLSY
310 320 330 340 350
RQLAEQLPAY VKELGFTHVE LMPVAEHPFG GSWGYQVTGF YAPTSRMGTP
360 370 380 390 400
DDFRFLVDAL HRAGIGVIVD WVPAHFPRDD WALAEFDGRP LYEHQDPRRA
410 420 430 440 450
AHPDWGTLEF DYGRKEVRNF LVANAVYWCQ EFHVDGLRAD AVASMLYLDY
460 470 480 490 500
SRDEGDWSPN AHGGREDLDA VALLQEMNAT VYRRFPGVVT IAEESTAWDG
510 520 530 540 550
VTRPTDSGGL GFGLKWNMGW MHDTLRYVSK EPVHRKYHHH DMTFGMVYAF
560 570 580 590 600
SENFVLPISH DEVVHGKRSL VSKMPGDWWQ QRATHRAYLG FMWAHPGKQL
610 620 630 640 650
LFMGQEFAQG SEWSETYGPD WWVLDSSYPA AGDHLGVRSL VRDLNRTYTA
660 670 680 690 700
SPALWERDSV PEGFAWVEAD AADDNVFAFL RFARDGSPLL CVSNFSPVVR
710 720 730 740 750
HGYRIGVPQE VGQWREVLNT DLEPYGGSGV HHARALRPEP VPAQGRAVSL
760
RMTLPPMATV WLRP
Length:764
Mass (Da):85,325
Last modified:October 1, 1996 - v1
Checksum:i6B45482E4A268ACF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11647 Genomic DNA. Translation: AAA67437.1.
PIRiS47569.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11647 Genomic DNA. Translation: AAA67437.1.
PIRiS47569.

3D structure databases

ProteinModelPortaliP52980.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning of the putative glycogen branching enzyme gene, glgB, from Streptomyces aureofaciens."
    Homerova D., Kormanec J.
    Biochim. Biophys. Acta 1200:334-336(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10762 / CCM 3239 / DSM 40127 / JCM 4008 / LMG 5968.

Entry informationi

Entry nameiGLGB_STRAU
AccessioniPrimary (citable) accession number: P52980
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.