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P52980 (GLGB_STRAU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
OrganismStreptomyces aureofaciens
Taxonomic identifier1894 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length764 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7647641,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188746

Sites

Active site4401Nucleophile By similarity
Active site4931Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P52980 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6B45482E4A268ACF

FASTA76485,325
        10         20         30         40         50         60 
MSAARQPSPT VRDKAAPEPA APAAPKGARA PRARRAAPPH GVRPAPALAA EERARLLEGR 

        70         80         90        100        110        120 
HHDPHAVLGA RTQRGGVAFR VLRPYAKAVT VVAKGLRTEL VDEGDGLFSG LLPLTGVPDY 

       130        140        150        160        170        180 
RLLVTYDSDE IEVHDPYRFL PALGELDLHL IGEGRHEELW TALGSQPMEH QGVAGTRFTV 

       190        200        210        220        230        240 
WAPNALGVRV TGDFSYWDAV AYPMRSLGAS GVWELFLPGV AEGALYKYEI TRPDGGRTLR 

       250        260        270        280        290        300 
ADPMARYAEV PPANASIVTA SRYEWQDAEW MARRGALAPH QAPMSVYELH LASWRPGLSY 

       310        320        330        340        350        360 
RQLAEQLPAY VKELGFTHVE LMPVAEHPFG GSWGYQVTGF YAPTSRMGTP DDFRFLVDAL 

       370        380        390        400        410        420 
HRAGIGVIVD WVPAHFPRDD WALAEFDGRP LYEHQDPRRA AHPDWGTLEF DYGRKEVRNF 

       430        440        450        460        470        480 
LVANAVYWCQ EFHVDGLRAD AVASMLYLDY SRDEGDWSPN AHGGREDLDA VALLQEMNAT 

       490        500        510        520        530        540 
VYRRFPGVVT IAEESTAWDG VTRPTDSGGL GFGLKWNMGW MHDTLRYVSK EPVHRKYHHH 

       550        560        570        580        590        600 
DMTFGMVYAF SENFVLPISH DEVVHGKRSL VSKMPGDWWQ QRATHRAYLG FMWAHPGKQL 

       610        620        630        640        650        660 
LFMGQEFAQG SEWSETYGPD WWVLDSSYPA AGDHLGVRSL VRDLNRTYTA SPALWERDSV 

       670        680        690        700        710        720 
PEGFAWVEAD AADDNVFAFL RFARDGSPLL CVSNFSPVVR HGYRIGVPQE VGQWREVLNT 

       730        740        750        760 
DLEPYGGSGV HHARALRPEP VPAQGRAVSL RMTLPPMATV WLRP 

« Hide

References

[1]"Cloning of the putative glycogen branching enzyme gene, glgB, from Streptomyces aureofaciens."
Homerova D., Kormanec J.
Biochim. Biophys. Acta 1200:334-336(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10762 / CCM 3239 / DSM 40127 / JCM 4008 / LMG 5968.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11647 Genomic DNA. Translation: AAA67437.1.
PIRS47569.

3D structure databases

ProteinModelPortalP52980.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_STRAU
AccessionPrimary (citable) accession number: P52980
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries