Reviewed, UniProtKB/Swiss-Prot P52979 (GLGB_AGRTU)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:

glgB

Organism

Agrobacterium tumefaciens

Taxonomic identifier

358 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188671
Sites
	Active site	312	1	By similarity
	Active site	347	1	By similarity
	Active site	352	1	By similarity
	Active site	415	1	By similarity
	Active site	417	1	By similarity
	Active site	470	1	By similarity
	Active site	537	1	By similarity
	Active site	538	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52979-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 70A3CD35A77F31E6	FASTA	734	83,623
	10 20 30 40 50 60 MKKPLNSAEE KKTGDITKAE IEAIKSGLHS NPFQIIPLHE TPEGFSARCF IPGAEEVSVL 70 80 90 100 110 120 TLDGNFVGEL KQIDPDGFFE GRIDLSKRQP VRYRACRDDA EWAVTDPYSF GPVLGPMDDY 130 140 150 160 170 180 FVREGSICGY STGWARIPLK LEGVEGFHFA VWAPNGRRVS VVGDFNNWDG RRHVMRFRKD 190 200 210 220 230 240 TGIWEIFAPD VYACAYKFEI LGANGELLPL KADPYARRGE LRPKNASVTA PELTQKWEDQ 250 260 270 280 290 300 AHREHWAQVD QRRQPISIYE VHAGSWQRSE DGTFLSWDEL EAQLIPYCTD MGFTHIEFLP 310 320 330 340 350 360 ITEHPYDPSW GYQTTGLYAP TARFGDPEGF ARFVNGAHKV GIGVLLDWVP AHFPTDEHGL 370 380 390 400 410 420 RWFDGTALYE HADPRQGFHP DWNTAIYNFG RIEVMSYLIN NALYWAEKFH LDGLRVDAVA 430 440 450 460 470 480 SMLYLDYSRK EGEWIPNEYG GRENLESVRF LQKMNSLVYG THPGVMTIAE ESTSWPKVSQ 490 500 510 520 530 540 PVHEGGLGFG FKWNMGFMHD TLSYFSREPV HRKFHHQELT FGLLYAFTEN FVLPLSHDEV 550 560 570 580 590 600 VHGKGSLIAK MSGDDWQKFA NLRSYYGFMW GYPGKKLLFM GQEFAQWSEW SEKGSLDWNL 610 620 630 640 650 660 RQYPMHEGMR RLVRDLNLTY RSKAALHARD CEPDGFRWLV VDDHENSVFA WLRTAPGEKP 670 680 690 700 710 720 VAVICNLTPV YRENYYVPLG VAGRWREILN TDAEIYGGSG KGNGGRVQAV DAGGEIGAML 730 VLPPLATIML EPEN

References

[1]

"Gene organization and transcription analysis of the Agrobacterium tumefaciens glycogen (glg) operon: two transcripts for the single phosphoglucomutase gene." Ugalde J.E., Lepek V., Uttaro A.D., Estrella J., Iglesias A., Ugalde R.A. J. Bacteriol. 180:6557-6564(1998) [PubMed: 9851999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A348.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF033856 Genomic DNA. Translation: AAD03472.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Enzyme and pathway databases
BRENDA	2.4.1.18. 2199.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 2 hits. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_AGRTU

Accession

Primary (citable) accession number: P52979

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)