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P52979 (GLGB_RHIRD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
OrganismRhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)
Taxonomic identifier358 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length734 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7347341,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188671

Sites

Active site4171Nucleophile By similarity
Active site4701Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P52979 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 70A3CD35A77F31E6

FASTA73483,623
        10         20         30         40         50         60 
MKKPLNSAEE KKTGDITKAE IEAIKSGLHS NPFQIIPLHE TPEGFSARCF IPGAEEVSVL 

        70         80         90        100        110        120 
TLDGNFVGEL KQIDPDGFFE GRIDLSKRQP VRYRACRDDA EWAVTDPYSF GPVLGPMDDY 

       130        140        150        160        170        180 
FVREGSICGY STGWARIPLK LEGVEGFHFA VWAPNGRRVS VVGDFNNWDG RRHVMRFRKD 

       190        200        210        220        230        240 
TGIWEIFAPD VYACAYKFEI LGANGELLPL KADPYARRGE LRPKNASVTA PELTQKWEDQ 

       250        260        270        280        290        300 
AHREHWAQVD QRRQPISIYE VHAGSWQRSE DGTFLSWDEL EAQLIPYCTD MGFTHIEFLP 

       310        320        330        340        350        360 
ITEHPYDPSW GYQTTGLYAP TARFGDPEGF ARFVNGAHKV GIGVLLDWVP AHFPTDEHGL 

       370        380        390        400        410        420 
RWFDGTALYE HADPRQGFHP DWNTAIYNFG RIEVMSYLIN NALYWAEKFH LDGLRVDAVA 

       430        440        450        460        470        480 
SMLYLDYSRK EGEWIPNEYG GRENLESVRF LQKMNSLVYG THPGVMTIAE ESTSWPKVSQ 

       490        500        510        520        530        540 
PVHEGGLGFG FKWNMGFMHD TLSYFSREPV HRKFHHQELT FGLLYAFTEN FVLPLSHDEV 

       550        560        570        580        590        600 
VHGKGSLIAK MSGDDWQKFA NLRSYYGFMW GYPGKKLLFM GQEFAQWSEW SEKGSLDWNL 

       610        620        630        640        650        660 
RQYPMHEGMR RLVRDLNLTY RSKAALHARD CEPDGFRWLV VDDHENSVFA WLRTAPGEKP 

       670        680        690        700        710        720 
VAVICNLTPV YRENYYVPLG VAGRWREILN TDAEIYGGSG KGNGGRVQAV DAGGEIGAML 

       730 
VLPPLATIML EPEN 

« Hide

References

[1]"Gene organization and transcription analysis of the Agrobacterium tumefaciens glycogen (glg) operon: two transcripts for the single phosphoglucomutase gene."
Ugalde J.E., Lepek V., Uttaro A.D., Estrella J., Iglesias A., Ugalde R.A.
J. Bacteriol. 180:6557-6564(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A348.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF033856 Genomic DNA. Translation: AAD03472.1.

3D structure databases

ProteinModelPortalP52979.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_RHIRD
AccessionPrimary (citable) accession number: P52979
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 15, 1998
Last modified: June 11, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries