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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei417 – 4171NucleophileBy similarity
Active sitei470 – 4701Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
OrganismiRhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)
Taxonomic identifieri358 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7347341,4-alpha-glucan branching enzyme GlgBPRO_0000188671Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP52979.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

P52979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPLNSAEE KKTGDITKAE IEAIKSGLHS NPFQIIPLHE TPEGFSARCF
60 70 80 90 100
IPGAEEVSVL TLDGNFVGEL KQIDPDGFFE GRIDLSKRQP VRYRACRDDA
110 120 130 140 150
EWAVTDPYSF GPVLGPMDDY FVREGSICGY STGWARIPLK LEGVEGFHFA
160 170 180 190 200
VWAPNGRRVS VVGDFNNWDG RRHVMRFRKD TGIWEIFAPD VYACAYKFEI
210 220 230 240 250
LGANGELLPL KADPYARRGE LRPKNASVTA PELTQKWEDQ AHREHWAQVD
260 270 280 290 300
QRRQPISIYE VHAGSWQRSE DGTFLSWDEL EAQLIPYCTD MGFTHIEFLP
310 320 330 340 350
ITEHPYDPSW GYQTTGLYAP TARFGDPEGF ARFVNGAHKV GIGVLLDWVP
360 370 380 390 400
AHFPTDEHGL RWFDGTALYE HADPRQGFHP DWNTAIYNFG RIEVMSYLIN
410 420 430 440 450
NALYWAEKFH LDGLRVDAVA SMLYLDYSRK EGEWIPNEYG GRENLESVRF
460 470 480 490 500
LQKMNSLVYG THPGVMTIAE ESTSWPKVSQ PVHEGGLGFG FKWNMGFMHD
510 520 530 540 550
TLSYFSREPV HRKFHHQELT FGLLYAFTEN FVLPLSHDEV VHGKGSLIAK
560 570 580 590 600
MSGDDWQKFA NLRSYYGFMW GYPGKKLLFM GQEFAQWSEW SEKGSLDWNL
610 620 630 640 650
RQYPMHEGMR RLVRDLNLTY RSKAALHARD CEPDGFRWLV VDDHENSVFA
660 670 680 690 700
WLRTAPGEKP VAVICNLTPV YRENYYVPLG VAGRWREILN TDAEIYGGSG
710 720 730
KGNGGRVQAV DAGGEIGAML VLPPLATIML EPEN
Length:734
Mass (Da):83,623
Last modified:December 14, 1998 - v2
Checksum:i70A3CD35A77F31E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033856 Genomic DNA. Translation: AAD03472.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033856 Genomic DNA. Translation: AAD03472.1.

3D structure databases

ProteinModelPortaliP52979.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Gene organization and transcription analysis of the Agrobacterium tumefaciens glycogen (glg) operon: two transcripts for the single phosphoglucomutase gene."
    Ugalde J.E., Lepek V., Uttaro A.D., Estrella J., Iglesias A., Ugalde R.A.
    J. Bacteriol. 180:6557-6564(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A348.

Entry informationi

Entry nameiGLGB_RHIRD
AccessioniPrimary (citable) accession number: P52979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: December 14, 1998
Last modified: September 30, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.