P52961 (NAR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
EC=2.4.2.31

Alternative name(s):
Mono(ADP-ribosyl)transferase 1
CD_antigen=CD296

Gene names

Name:

ART1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has ADP-ribosyltransferase activity toward GLP1R. Ref.5
Catalytic activity	NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP+ + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Sarcoplasmic reticulum membrane; Lipid-anchor › GPI-anchor.
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.

Ontologies

Keywords
Cellular component	Membrane Sarcoplasmic reticulum
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	NAD NADP
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein ADP-ribosylation Traceable author statement. Source: ProtInc
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement. Source: ProtInc sarcoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+-protein-arginine ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC NAD+ ADP-ribosyltransferase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 295	273	GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1		PRO_0000019311
	Propeptide	296 – 327	32	Removed in mature form Potential		PRO_0000019312
Sites
	Active site	247	1	By similarity
	Binding site	121	1	NAD By similarity
	Binding site	179	1	NAD By similarity
	Binding site	233	1	NAD By similarity
Amino acid modifications
	Lipidation	295	1	GPI-anchor amidated serine Potential
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	253	1	N-linked (GlcNAc...) Potential
	Disulfide bond	53 ↔ 277		By similarity
	Disulfide bond	174 ↔ 224		By similarity
Natural variations
	Natural variant	105	1	P → L. Corresponds to variant rs35123761 [ dbSNP | Ensembl ].		VAR_034125
	Natural variant	126	1	P → R. Corresponds to variant rs35619488 [ dbSNP | Ensembl ].		VAR_034126
	Natural variant	257	1	L → P. Ref.1 Corresponds to variant rs2280134 [ dbSNP | Ensembl ].		VAR_053526

Sequences

	Sequence		Length	Mass (Da)
	P52961 [UniParc]. Last modified September 23, 2008. Version 2. Checksum: 8FDC568197031EA5	FASTA	327	36,335
	10 20 30 40 50 60 MQMPAMMSLL LVSVGLMEAL QAQSHPITRR DLFSQEIQLD MALASFDDQY AGCAAAMTAA 70 80 90 100 110 120 LPDLNHTEFQ ANQVYADSWT LASSQWQERQ ARWPEWSLSP TRPSPPPLGF RDEHGVALLA 130 140 150 160 170 180 YTANSPLHKE FNAAVREAGR SRAHYLHHFS FKTLHFLLTE ALQLLGSGQR PPRCHQVFRG 190 200 210 220 230 240 VHGLRFRPAG PRATVRLGGF ASASLKHVAA QQFGEDTFFG IWTCLGAPIK GYSFFPGEEE 250 260 270 280 290 300 VLIPPFETFQ VINASRLAQG PARIYLRALG KHSTYNCEYI KDKKCKSGPC HLDNSAMGQS 310 320 PLSAVWSLLL LLWFLVVRAF PDGPGLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Immunological and structural conservation of mammalian skeletal muscle glycosylphosphatidylinositol-linked ADP-ribosyltransferases." Okazaki I.J., Zolkiewska A., Nightingale M.S., Moss J. Biochemistry 33:12828-12836(1994) [PubMed: 7947688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PRO-257. Tissue: Skeletal muscle.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Conservation of the ART gene family across mammalian species." Kuehl M., Glowacki G., Haag F., Koch-Nolte F. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-223.
[5]	"Glucagon like-peptide-1 receptor is covalently modified by endogenous mono-ADP-ribosyltransferase." Dezelak M., Bavec A. Mol. Biol. Rep. 0:0-0(2011) [PubMed: 21901419] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S74683 mRNA. Translation: AAB32387.1. CH471158 Genomic DNA. Translation: EAX02559.1. BC069102 mRNA. Translation: AAH69102.1. BC111729 mRNA. Translation: AAI11730.1. AJ291430 mRNA. Translation: CAC69964.1.
IPI	IPI00006067.
PIR	A55966.
RefSeq	NP_004305.2. NM_004314.2.
UniGene	Hs.382188.
3D structure databases
ProteinModelPortal	P52961.
ModBase	Search...
Protein-protein interaction databases
STRING	P52961.
PTM databases
PhosphoSite	P52961.
Polymorphism databases
DMDM	206729882.
Proteomic databases
PRIDE	P52961.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000250693; ENSP00000250693; ENSG00000129744.
GeneID	417.
KEGG	hsa:417.
Organism-specific databases
CTD	417.
GeneCards	GC11P003666.
H-InvDB	HIX0035913.
HGNC	HGNC:723. ART1.
MIM	601625. gene.
neXtProt	NX_P52961.
PharmGKB	PA25014.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19334.
GeneTree	ENSGT00530000062975.
HOGENOM	HBG443999.
HOVERGEN	HBG004464.
InParanoid	P52961.
OMA	AQGPARI.
OrthoDB	EOG4G4GQW.
PhylomeDB	P52961.
Gene expression databases
ArrayExpress	P52961.
Bgee	P52961.
CleanEx	HS_ART1.
Genevestigator	P52961.
GermOnline	ENSG00000129744. Homo sapiens.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
KO	K06716.
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
PROSITE	PS01291. ART. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00102. Becaplermin.
SOURCE	Search...

Entry information

Entry name

NAR1_HUMAN

Accession

Primary (citable) accession number: P52961
Secondary accession number(s): Q6NTD2, Q96KT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.