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Protein

Peroxisome proliferation transcriptional regulator

Gene

PIP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The PIP2-OAF1 heterodimer acts as a transcriptional activator to induce the transcription of genes encoding proteins involved in fatty acid beta-oxidation, a response called oleic acid induction, when cells grow on fatty acids as sole carbon source. Recognizes and binds to the oleate response element (ORE) (or peroxisome box), two inverted CGG triplets spaced by 14 to 18 intervening nucleotides, in the promoter region of a number of genes (such as CTA1, FOX1 to FOX3, FAA2, PAS8, PAS10, etc.) for peroxisomal proteins. Activity is inhibited by OAF1 under non-inducing conditions. Activity is repressed by glucose.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi25 – 5228Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • positive regulation of fatty acid beta-oxidation Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter by oleic acid Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33833-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisome proliferation transcriptional regulator
Alternative name(s):
Oleate-activated transcription factor 2
Gene namesi
Name:PIP2
Synonyms:OAF2
Ordered Locus Names:YOR363C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR363C.
SGDiS000005890. PIP2.

Subcellular locationi

GO - Cellular componenti

  • Pip2-Oaf1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 996996Peroxisome proliferation transcriptional regulatorPRO_0000114964Add
BLAST

Proteomic databases

MaxQBiP52960.
PRIDEiP52960.

PTM databases

iPTMnetiP52960.

Expressioni

Inductioni

Autoregulated. Induced upon growth on fatty acids.2 Publications

Interactioni

Subunit structurei

Heterodimer of PIP2 and OAF1.

Protein-protein interaction databases

BioGridi34748. 21 interactions.
DIPiDIP-1013N.
IntActiP52960. 1 interaction.
MINTiMINT-2784690.

Structurei

3D structure databases

ProteinModelPortaliP52960.
SMRiP52960. Positions 23-80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi985 – 99399aaTAD

Domaini

the 9aaTAD motif (residues 985 to 993) is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000000033.
HOGENOMiHOG000066010.
InParanoidiP52960.
OMAiKPRCGRC.
OrthoDBiEOG7HF1SV.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR002409. Aflatoxin_BRP.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
PRINTSiPR00755. AFLATOXINBRP.
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYFTDESSPA MNRVGKKRNR LSFVCQACRK AKTKCDQEKP RCGRCTKQNL
60 70 80 90 100
FCIYDVARQA APRNPNKDAT IARLKKEIRY WRNKTVDLTQ EKKDFYTALK
110 120 130 140 150
RPTEELAARR TCKSLQENSF PISLYKTHPR LIMTKVMKRE INPLSEKYLI
160 170 180 190 200
FQDTFLKTLI ASVLLSCSRN SMIPALNADI SRSRTQPCVK NNVVKMREVL
210 220 230 240 250
LKNSKYESQR KSINEFTDRL LQRKNPEEQI AVNKVISLLY SNRESSYLED
260 270 280 290 300
TCPTENDYSD LLKGYINEIE KTLPPKAIIE QYLSHFFEHI FHLIPFASKE
310 320 330 340 350
MLEESIHTTV QYNELGEVRL SMGTTLIRNK MENLCILLLI LRIAYISLTF
360 370 380 390 400
IEDKIEDYSP YITKEMLEQY PIQSEVIFLA QQILASENWC ACANENTISC
410 420 430 440 450
LLYIWCAFVF SPTEGDFLLE QPSDVIINLV ILIGTSIGLH RDPSDFPALN
460 470 480 490 500
HPEASDKRLL NLRRIQWLSI ISMATLESSL KGRLLVSPLS MIDLFIDVRD
510 520 530 540 550
PNCVEIYKKR VKKDLTGSES DEQLLEIHEI FFHRAQLALF LSDLNNITIS
560 570 580 590 600
YSGSVPMDTL ETLRVKANEL LKNKFQLRSV DINIYDEEKT FQKLTFNSIL
610 620 630 640 650
NSISLSGQIL GKLMMLRASI ALMLYFETLA MERSECLSFF YKYFFQCCAD
660 670 680 690 700
TISLIRFFFL YFNGSYEKVL SSLVCFITTK VIQLAVPTTM FTLLVIIMRV
710 720 730 740 750
ELAKNMLLVK CNECNARGDI SDLPEIKEKI KSLDTIKENF ERLLLEVYLL
760 770 780 790 800
ASQNLRFKYF YIFKMLTLFD VFIQRLRKGQ LFSGLFVKVD KDLTTKKIAT
810 820 830 840 850
MLELTLGINL DKSDHLIDRL KGKNLTVNFT LDQLYQIIKE FDRIKNIGVA
860 870 880 890 900
DPQNSLNPSK PNMKDNTPTI ELLLNSSVEN ESVPPYSSSN DPTNVGNAST
910 920 930 940 950
YSLAHNISNQ NNEENMPPSI GSSESNRAAP NLNFMPINNN YNNSGSNINN
960 970 980 990
NDNVKLPSNF KNYYDPPMSS LDISMDVPDI FGSLDFFDYD LLFQND
Length:996
Mass (Da):114,711
Last modified:October 1, 1996 - v1
Checksum:iA4E3895746EFCF3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91991 Genomic DNA. Translation: CAA63046.1.
Z75271 Genomic DNA. Translation: CAA99692.1.
BK006948 Genomic DNA. Translation: DAA11124.1.
PIRiS70646.
RefSeqiNP_015008.3. NM_001183783.3.

Genome annotation databases

EnsemblFungiiYOR363C; YOR363C; YOR363C.
GeneIDi854545.
KEGGisce:YOR363C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91991 Genomic DNA. Translation: CAA63046.1.
Z75271 Genomic DNA. Translation: CAA99692.1.
BK006948 Genomic DNA. Translation: DAA11124.1.
PIRiS70646.
RefSeqiNP_015008.3. NM_001183783.3.

3D structure databases

ProteinModelPortaliP52960.
SMRiP52960. Positions 23-80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34748. 21 interactions.
DIPiDIP-1013N.
IntActiP52960. 1 interaction.
MINTiMINT-2784690.

PTM databases

iPTMnetiP52960.

Proteomic databases

MaxQBiP52960.
PRIDEiP52960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR363C; YOR363C; YOR363C.
GeneIDi854545.
KEGGisce:YOR363C.

Organism-specific databases

EuPathDBiFungiDB:YOR363C.
SGDiS000005890. PIP2.

Phylogenomic databases

GeneTreeiENSGT00390000000033.
HOGENOMiHOG000066010.
InParanoidiP52960.
OMAiKPRCGRC.
OrthoDBiEOG7HF1SV.

Enzyme and pathway databases

BioCyciYEAST:G3O-33833-MONOMER.

Miscellaneous databases

PROiP52960.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR002409. Aflatoxin_BRP.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
PRINTSiPR00755. AFLATOXINBRP.
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Pip2p: a transcriptional regulator of peroxisome proliferation in the yeast Saccharomyces cerevisiae."
    Rottensteiner H., Kal A.J., Filipits M., Binder M., Hamilton B., Tabak H.F., Ruis H.
    EMBO J. 15:2924-2934(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DNA-BINDING.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A heterodimer of the Zn2Cys6 transcription factors Pip2p and Oaf1p controls induction of genes encoding peroxisomal proteins in Saccharomyces cerevisiae."
    Rottensteiner H., Kal A.J., Hamilton B., Ruis H., Tabak H.F.
    Eur. J. Biochem. 247:776-783(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH OAF1, INDUCTION.
  5. "A complex containing two transcription factors regulates peroxisome proliferation and the coordinate induction of beta-oxidation enzymes in Saccharomyces cerevisiae."
    Karpichev I.V., Luo Y., Marians R.C., Small G.M.
    Mol. Cell. Biol. 17:69-80(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH OAF1, INDUCTION.
  6. "Functional analysis of the Zn(2)Cys(6) transcription factors Oaf1p and Pip2p. Different roles in fatty acid induction of beta-oxidation in Saccharomyces cerevisiae."
    Baumgartner U., Hamilton B., Piskacek M., Ruis H., Rottensteiner H.
    J. Biol. Chem. 274:22208-22216(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Saccharomyces cerevisiae Pip2p-Oaf1p regulates PEX25 transcription through an adenine-less ORE."
    Rottensteiner H., Hartig A., Hamilton B., Ruis H., Erdmann R., Gurvitz A.
    Eur. J. Biochem. 270:2013-2022(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.
  8. "Nine-amino-acid transactivation domain: establishment and prediction utilities."
    Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
    Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.

Entry informationi

Entry nameiPIP2_YEAST
AccessioniPrimary (citable) accession number: P52960
Secondary accession number(s): D6W358
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.