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P52957 (AFLR_EMENI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sterigmatocystin biosynthesis regulatory protein
Gene names
Name:aflR
ORF Names:AN7820
OrganismEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]
Taxonomic identifier227321 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length433 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of sterigmatocystin biosynthesis. Ref.2

Pathway

Mycotoxin biosynthesis; sterigmatocystin biosynthesis [regulation].

Subcellular location

Nucleus By similarity.

Sequence similarities

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
Metal-binding
Zinc
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaflatoxin biosynthetic process

Inferred from electronic annotation. Source: InterPro

asperthecin biosynthetic process

Inferred from genetic interaction PubMed 22283524. Source: ASPGD

monodictyphenone biosynthetic process

Inferred from mutant phenotype PubMed 20139316. Source: ASPGD

positive regulation of asperthecin biosynthetic process

Inferred from genetic interaction PubMed 22283524. Source: ASPGD

positive regulation of secondary metabolite biosynthetic process

Inferred from mutant phenotype Ref.2. Source: ASPGD

positive regulation of sterigmatocystin biosynthetic process

Inferred from mutant phenotype Ref.2. Source: ASPGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype Ref.2. Source: ASPGD

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 9680223. Source: ASPGD

regulation of secondary metabolic process

Inferred from sequence or structural similarity PubMed 21351751. Source: ASPGD

sterigmatocystin biosynthetic process

Inferred from mutant phenotype Ref.2. Source: ASPGD

   Cellular_componentnucleus

Inferred by curator PubMed 9680223. Source: ASPGD

   Molecular_functionsequence-specific DNA binding

Inferred from direct assay PubMed 9680223. Source: ASPGD

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 9680223. Source: ASPGD

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 433433Sterigmatocystin biosynthesis regulatory protein
PRO_0000114934

Regions

DNA binding28 – 5528Zn(2)-C6 fungal-type
Compositional bias381 – 39010Poly-Ser

Experimental info

Sequence conflict3401S → T in AAC49195. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P52957 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: C9B8A0F83799472D

FASTA43346,566
        10         20         30         40         50         60 
MEPPAISQQS TPTAPGGTQG TRKLRESCIS CSRSKVKCNK EKPTCSRCVR RGLPCEYMVS 

        70         80         90        100        110        120 
RRTGRTRVIG VEQPKTAPSP TTPTNTTAAT TATKAGPPVT TDSAVHTPVI TTAPSPKPVQ 

       130        140        150        160        170        180 
IQSPPAEPDL WGAILSPNTS TSTDLSSLLS VNTNFSQLFA SLSPSLLEGM DGMDAEMHAP 

       190        200        210        220        230        240 
ELGALSVADP SSSMMQGLEA PNAAQPPSSN TTSHSYCLSI CLDTLMRLFP NAGANCERPG 

       250        260        270        280        290        300 
HESNPGKLFT IESVIEDNKQ ILDTAQTILA CRCAEDEYVV TLVSLIVFKV LGWYVAAARD 

       310        320        330        340        350        360 
RSSDPGREED FNWSTAQDSR RGSVSSFEEQ VLHLPTVVGS YCIDGHHQSR MAAQLVLSEL 

       370        380        390        400        410        420 
YRVQRLVTQV SRRLESIRRR SSSSSSSASS NTTDSDGGMS TPLSSTTLVH LEDDLRKRLR 

       430 
AVSSETISIL RHA 

« Hide

References

« Hide 'large scale' references
[1]"Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans."
Brown D.W., Yu J.-H., Kelkar H.S., Fernandes M., Nesbitt T.C., Keller N.P., Adams T.H., Leonard T.J.
Proc. Natl. Acad. Sci. U.S.A. 93:1418-1422(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: FGSC 26.
[2]"Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus."
Yu J.-H., Butchko R.A.E., Fernandes M., Keller N.P., Leonard T.J., Adams T.H.
Curr. Genet. 29:549-555(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae."
Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. expand/collapse author list , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.
Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[4]"The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. expand/collapse author list , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U34740 Genomic DNA. Translation: AAC49195.1.
AACD01000132 Genomic DNA. Translation: EAA61608.1.
BN001304 Genomic DNA. Translation: CBF80174.1.
PIRS69999.
RefSeqXP_681089.1. XM_675997.1.

3D structure databases

ProteinModelPortalP52957.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING162425.CADANIAP00000963.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANIAT00000963; CADANIAP00000963; CADANIAG00000963.
GeneID2869641.
KEGGani:AN7820.2.

Phylogenomic databases

eggNOGNOG289594.
HOGENOMHOG000033838.
OMANTNEDEH.
OrthoDBEOG77M8Z1.

Enzyme and pathway databases

UniPathwayUPA00377.

Family and domain databases

Gene3D4.10.240.10. 1 hit.
InterProIPR002409. Aflatoxin_BRP.
IPR013700. AflR.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamPF08493. AflR. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
PRINTSPR00755. AFLATOXINBRP.
SMARTSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMSSF57701. SSF57701. 1 hit.
PROSITEPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAFLR_EMENI
AccessionPrimary (citable) accession number: P52957
Secondary accession number(s): C8VDU4, Q5AV60
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 1, 2007
Last modified: June 11, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways