P52957 (AFLR_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sterigmatocystin biosynthesis regulatory protein

Gene names

Name:	aflR
ORF Names:	AN7820

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella › Emericella nidulans › mitosporic Emericella nidulans

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the regulation of sterigmatocystin biosynthesis. Ref.2
Pathway	Mycotoxin biosynthesis; sterigmatocystin biosynthesis [regulation].
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aflatoxin biosynthetic process Inferred from electronic annotation. Source: InterPro regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: GOC sterigmatocystin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Sterigmatocystin biosynthesis regulatory protein		PRO_0000114934
Regions
	DNA binding	28 – 55	28	Zn(2)-C6 fungal-type
	Compositional bias	381 – 390	10	Poly-Ser
Experimental info
	Sequence conflict	340	1	S → T in AAC49195. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52957 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: C9B8A0F83799472D	FASTA	433	46,566
	10 20 30 40 50 60 MEPPAISQQS TPTAPGGTQG TRKLRESCIS CSRSKVKCNK EKPTCSRCVR RGLPCEYMVS 70 80 90 100 110 120 RRTGRTRVIG VEQPKTAPSP TTPTNTTAAT TATKAGPPVT TDSAVHTPVI TTAPSPKPVQ 130 140 150 160 170 180 IQSPPAEPDL WGAILSPNTS TSTDLSSLLS VNTNFSQLFA SLSPSLLEGM DGMDAEMHAP 190 200 210 220 230 240 ELGALSVADP SSSMMQGLEA PNAAQPPSSN TTSHSYCLSI CLDTLMRLFP NAGANCERPG 250 260 270 280 290 300 HESNPGKLFT IESVIEDNKQ ILDTAQTILA CRCAEDEYVV TLVSLIVFKV LGWYVAAARD 310 320 330 340 350 360 RSSDPGREED FNWSTAQDSR RGSVSSFEEQ VLHLPTVVGS YCIDGHHQSR MAAQLVLSEL 370 380 390 400 410 420 YRVQRLVTQV SRRLESIRRR SSSSSSSASS NTTDSDGGMS TPLSSTTLVH LEDDLRKRLR 430 AVSSETISIL RHA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans." Brown D.W., Yu J.-H., Kelkar H.S., Fernandes M., Nesbitt T.C., Keller N.P., Adams T.H., Leonard T.J. Proc. Natl. Acad. Sci. U.S.A. 93:1418-1422(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FGSC 26.
[2]	"Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus." Yu J.-H., Butchko R.A.E., Fernandes M., Keller N.P., Leonard T.J., Adams T.H. Curr. Genet. 29:549-555(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[4]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34740 Genomic DNA. Translation: AAC49195.1. AACD01000132 Genomic DNA. Translation: EAA61608.1. BN001304 Genomic DNA. Translation: CBF80174.1.
PIR	S69999.
RefSeq	XP_681089.1. XM_675997.1.
3D structure databases
ProteinModelPortal	P52957.
ModBase	Search...
Protein-protein interaction databases
STRING	162425.CADANIAP00000963.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANIAT00000963; CADANIAP00000963; CADANIAG00000963.
GeneID	2869641.
KEGG	ani:AN7820.2.
Phylogenomic databases
eggNOG	NOG289594.
HOGENOM	HOG000033838.
OMA	AYASESS.
OrthoDB	EOG4T4H59.
Enzyme and pathway databases
UniPathway	UPA00377.
Family and domain databases
Gene3D	4.10.240.10. 1 hit.
InterPro	IPR002409. Aflatoxin_BRP. IPR013700. AflR. IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
Pfam	PF08493. AflR. 1 hit. PF00172. Zn_clus. 1 hit. [Graphical view]
PRINTS	PR00755. AFLATOXINBRP.
SMART	SM00066. GAL4. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
PROSITE	PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFLR_EMENI

Accession

Primary (citable) accession number: P52957
Secondary accession number(s): C8VDU4, Q5AV60

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 1, 2007
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program