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P52955 (LBX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor LBX1
Alternative name(s):
Ladybird homeobox protein homolog 1
Gene names
Name:Lbx1
Synonyms:Lbx1h
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor required for the development of GABAergic interneurons in the dorsal horn of the spinal cord and migration and further development of hypaxial muscle precursor cells for limb muscles, diaphragm and hypoglossal cord. Ref.6 Ref.7 Ref.9

Subunit structure

Interacts with SKOR1 which acts as a transcriptional corepressor. Ref.8

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in the dorsal part of the spinal cord and hindbrain and in presumptive myogenic cells in lateral regions of differentiating somites. Ref.1

Developmental stage

Expressed in the developing central nervous system from 10.5 dpc to 16.5 dpc. Expressed in presumptive myogenic cells from 9.5 dpc until 16.5 dpc with highest levels at 10.5-11.5 dpc. Ref.1

Disruption phenotype

Death at birth. Mice fail to expand their lungs and do not move their abnormally thin limbs. Ref.6

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Sequence caution

The sequence BAE25939.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processDifferentiation
Myogenesis
Neurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processheart looping

Inferred from mutant phenotype PubMed 12522123. Source: MGI

muscle organ development

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 12522123. Source: MGI

negative regulation of neuron differentiation

Inferred from mutant phenotype Ref.9. Source: MGI

neuron fate commitment

Inferred from mutant phenotype Ref.7. Source: MGI

neuron fate determination

Inferred from mutant phenotype Ref.9. Source: MGI

regulation of transcription from RNA polymerase II promoter involved in spinal cord association neuron specification

Inferred from mutant phenotype PubMed 12062038. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay Ref.8. Source: MGI

spinal cord motor neuron differentiation

Inferred from genetic interaction PubMed 15769945. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcription factor complex

Inferred from direct assay Ref.8. Source: MGI

   Molecular_functionsequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.8. Source: MGI

transcription regulatory region sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Skor1Q8BX462EBI-604594,EBI-604451

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282Transcription factor LBX1
PRO_0000049167

Regions

DNA binding125 – 18460Homeobox
Compositional bias219 – 2279Poly-Gly
Compositional bias271 – 28212Asp/Glu-rich (highly acidic)

Experimental info

Sequence conflict33 – 7240LTPFS…PGGLP → YAVQHRGHPQQAVRAEKLLA VWGGAPAGGRGQARAGRLA in CAA62343. Ref.1
Sequence conflict2251Missing in CAA62343. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P52955 [UniParc].

Last modified November 14, 2006. Version 2.
Checksum: 3A4A80CD47B0228C

FASTA28230,262
        10         20         30         40         50         60 
MTSKEDGKAA PGEERRRSPL DHLPPPANSN KPLTPFSIED ILNKPSVRRS YSLCGAAHLL 

        70         80         90        100        110        120 
AAADKHAPGG LPLAGRALLS QTSPLCALEE LASKTFKGLE VSVLQAAEGR DGMTIFGQRQ 

       130        140        150        160        170        180 
TPKKRRKSRT AFTNHQIYEL EKRFLYQKYL SPADRDQIAQ QLGLTNAQVI TWFQNRRAKL 

       190        200        210        220        230        240 
KRDLEEMKAD VESAKKLGPS GQMDIVALAE LEQNSEASGG GGGGGCGRAK SRPGSPALPP 

       250        260        270        280 
GAPQAPGGGP LQLSPASPLT DQRASSQDCS EDEEDEEIDV DD 

« Hide

References

« Hide 'large scale' references
[1]"Mouse Lbx1 and human LBX1 define a novel mammalian homeobox gene family related to the Drosophila lady bird genes."
Jagla K., Dolle P., Mattei M.-G., Jagla T., Schuhbaur B., Dretzen G., Bellard F., Bellard M.
Mech. Dev. 53:345-356(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: BALB/c.
[2]Jagla K.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Mouse homeobox gene Lbx1 in myogenesis."
Kurose T., Endo T.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-282.
Strain: C57BL/6J.
Tissue: Medulla oblongata.
[6]"The role of Lbx1 in migration of muscle precursor cells."
Brohmann H., Jagla K., Birchmeier C.
Development 127:437-445(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]"The homeodomain factor lbx1 distinguishes two major programs of neuronal differentiation in the dorsal spinal cord."
Muller T., Brohmann H., Pierani A., Heppenstall P.A., Lewin G.R., Jessell T.M., Birchmeier C.
Neuron 34:551-562(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Corl1, a novel neuronal lineage-specific transcriptional corepressor for the homeodomain transcription factor Lbx1."
Mizuhara E., Nakatani T., Minaki Y., Sakamoto Y., Ono Y.
J. Biol. Chem. 280:3645-3655(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKOR1.
[9]"Lbx1 and Tlx3 are opposing switches in determining GABAergic versus glutamatergic transmitter phenotypes."
Cheng L., Samad O.A., Xu Y., Mizuguchi R., Luo P., Shirasawa S., Goulding M., Ma Q.
Nat. Neurosci. 8:1510-1515(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X90829 mRNA. Translation: CAA62343.1.
AB108497 mRNA. Translation: BAC75634.1.
BC119177 mRNA. Translation: AAI19178.1.
BC120586 mRNA. Translation: AAI20587.1.
AK144562 mRNA. Translation: BAE25939.1. Different initiation.
RefSeqNP_034821.2. NM_010691.5.
XP_006526785.1. XM_006526722.1.
UniGeneMm.116815.

3D structure databases

ProteinModelPortalP52955.
SMRP52955. Positions 124-182.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201117. 1 interaction.
IntActP52955. 2 interactions.

Proteomic databases

PRIDEP52955.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000099401; ENSMUSP00000096997; ENSMUSG00000025216.
GeneID16814.
KEGGmmu:16814.
UCSCuc008hqv.1. mouse.

Organism-specific databases

CTD10660.
MGIMGI:104867. Lbx1.

Phylogenomic databases

eggNOGNOG240187.
GeneTreeENSGT00740000114906.
HOGENOMHOG000007247.
HOVERGENHBG006244.
InParanoidP52955.
KOK09353.
OMANSEATAG.
OrthoDBEOG7X3QRB.
PhylomeDBP52955.
TreeFamTF325047.

Gene expression databases

BgeeP52955.
CleanExMM_LBX1.
GenevestigatorP52955.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00031. HTHREPRESSR.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio290692.
PROP52955.
SOURCESearch...

Entry information

Entry nameLBX1_MOUSE
AccessionPrimary (citable) accession number: P52955
Secondary accession number(s): Q3UN09, Q80XA3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 14, 2006
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot