P52955 (LBX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor LBX1

Alternative name(s):
Ladybird homeobox protein homolog 1

Gene names

Name:	Lbx1
Synonyms:	Lbx1h

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor required for the development of GABAergic interneurons in the dorsal horn of the spinal cord and migration and further development of hypaxial muscle precursor cells for limb muscles, diaphragm and hypoglossal cord. Ref.6 Ref.7 Ref.9
Subunit structure	Interacts with SKOR1 which acts as a transcriptional corepressor. Ref.8
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in the dorsal part of the spinal cord and hindbrain and in presumptive myogenic cells in lateral regions of differentiating somites. Ref.1
Developmental stage	Expressed in the developing central nervous system from 10.5 dpc to 16.5 dpc. Expressed in presumptive myogenic cells from 9.5 dpc until 16.5 dpc with highest levels at 10.5-11.5 dpc. Ref.1
Disruption phenotype	Death at birth. Mice fail to expand their lungs and do not move their abnormally thin limbs. Ref.6
Sequence similarities	Contains 1 homeobox DNA-binding domain.
Sequence caution	The sequence BAE25939.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Differentiation Myogenesis Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heart looping Inferred from mutant phenotype PubMed 12522123. Source: MGI muscle organ development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell proliferation Inferred from mutant phenotype PubMed 12522123. Source: MGI negative regulation of neuron differentiation Inferred from mutant phenotype Ref.9. Source: MGI neuron fate determination Inferred from mutant phenotype Ref.9. Source: MGI regulation of transcription from RNA polymerase II promoter involved in spinal cord association neuron specification Inferred from mutant phenotype PubMed 12062038. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	transcription factor complex Inferred from direct assay Ref.8. Source: MGI
Molecular_function	sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.8. Source: MGI transcription regulatory region sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Skor1	Q8BX46	2	EBI-604594,EBI-604451

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Transcription factor LBX1		PRO_0000049167
Regions
	DNA binding	125 – 184	60	Homeobox
	Compositional bias	219 – 227	9	Poly-Gly
	Compositional bias	271 – 282	12	Asp/Glu-rich (highly acidic)
Experimental info
	Sequence conflict	33 – 72	40	LTPFS…PGGLP → YAVQHRGHPQQAVRAEKLLA VWGGAPAGGRGQARAGRLA in CAA62343. Ref.1
	Sequence conflict	225	1	Missing in CAA62343. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52955 [UniParc]. Last modified November 14, 2006. Version 2. Checksum: 3A4A80CD47B0228C	FASTA	282	30,262
	10 20 30 40 50 60 MTSKEDGKAA PGEERRRSPL DHLPPPANSN KPLTPFSIED ILNKPSVRRS YSLCGAAHLL 70 80 90 100 110 120 AAADKHAPGG LPLAGRALLS QTSPLCALEE LASKTFKGLE VSVLQAAEGR DGMTIFGQRQ 130 140 150 160 170 180 TPKKRRKSRT AFTNHQIYEL EKRFLYQKYL SPADRDQIAQ QLGLTNAQVI TWFQNRRAKL 190 200 210 220 230 240 KRDLEEMKAD VESAKKLGPS GQMDIVALAE LEQNSEASGG GGGGGCGRAK SRPGSPALPP 250 260 270 280 GAPQAPGGGP LQLSPASPLT DQRASSQDCS EDEEDEEIDV DD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse Lbx1 and human LBX1 define a novel mammalian homeobox gene family related to the Drosophila lady bird genes." Jagla K., Dolle P., Mattei M.-G., Jagla T., Schuhbaur B., Dretzen G., Bellard F., Bellard M. Mech. Dev. 53:345-356(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: BALB/c.
[2]	Jagla K. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Mouse homeobox gene Lbx1 in myogenesis." Kurose T., Endo T. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-282. Strain: C57BL/6J. Tissue: Medulla oblongata.
[6]	"The role of Lbx1 in migration of muscle precursor cells." Brohmann H., Jagla K., Birchmeier C. Development 127:437-445(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"The homeodomain factor lbx1 distinguishes two major programs of neuronal differentiation in the dorsal spinal cord." Muller T., Brohmann H., Pierani A., Heppenstall P.A., Lewin G.R., Jessell T.M., Birchmeier C. Neuron 34:551-562(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Corl1, a novel neuronal lineage-specific transcriptional corepressor for the homeodomain transcription factor Lbx1." Mizuhara E., Nakatani T., Minaki Y., Sakamoto Y., Ono Y. J. Biol. Chem. 280:3645-3655(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKOR1.
[9]	"Lbx1 and Tlx3 are opposing switches in determining GABAergic versus glutamatergic transmitter phenotypes." Cheng L., Samad O.A., Xu Y., Mizuguchi R., Luo P., Shirasawa S., Goulding M., Ma Q. Nat. Neurosci. 8:1510-1515(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X90829 mRNA. Translation: CAA62343.1. AB108497 mRNA. Translation: BAC75634.1. BC119177 mRNA. Translation: AAI19178.1. BC120586 mRNA. Translation: AAI20587.1. AK144562 mRNA. Translation: BAE25939.1. Different initiation.
IPI	IPI00132589.
RefSeq	NP_034821.2. NM_010691.5.
UniGene	Mm.116815.
3D structure databases
ProteinModelPortal	P52955.
SMR	P52955. Positions 123-182.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52955. 2 interactions.
Proteomic databases
PRIDE	P52955.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000099401; ENSMUSP00000096997; ENSMUSG00000025216.
GeneID	16814.
KEGG	mmu:16814.
UCSC	uc008hqv.1. mouse.
Organism-specific databases
CTD	10660.
MGI	MGI:104867. Lbx1.
Phylogenomic databases
eggNOG	NOG240187.
GeneTree	ENSGT00700000104206.
HOGENOM	HOG000007247.
HOVERGEN	HBG006244.
InParanoid	P52955.
KO	K09353.
OMA	NSEATAG.
OrthoDB	EOG4TTGJP.
Gene expression databases
Bgee	P52955.
CleanEx	MM_LBX1.
Genevestigator	P52955.
GermOnline	ENSMUSG00000025216. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR000047. HTH_motif. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00031. HTHREPRESSR.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	290692.
SOURCE	Search...

Entry information

Entry name

LBX1_MOUSE

Accession

Primary (citable) accession number: P52955
Secondary accession number(s): Q3UN09, Q80XA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 14, 2006
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program