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Protein

Transcription factor LBX1

Gene

Lbx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor required for the development of GABAergic interneurons in the dorsal horn of the spinal cord and migration and further development of hypaxial muscle precursor cells for limb muscles, diaphragm and hypoglossal cord.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi125 – 18460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. heart looping Source: MGI
  2. muscle organ development Source: UniProtKB-KW
  3. negative regulation of cell proliferation Source: MGI
  4. negative regulation of neuron differentiation Source: MGI
  5. neuron fate commitment Source: MGI
  6. neuron fate determination Source: MGI
  7. regulation of transcription, DNA-templated Source: MGI
  8. regulation of transcription from RNA polymerase II promoter involved in spinal cord association neuron specification Source: MGI
  9. spinal cord motor neuron differentiation Source: MGI
  10. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Myogenesis, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor LBX1
Alternative name(s):
Ladybird homeobox protein homolog 1
Gene namesi
Name:Lbx1
Synonyms:Lbx1h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:104867. Lbx1.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Death at birth. Mice fail to expand their lungs and do not move their abnormally thin limbs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Transcription factor LBX1PRO_0000049167Add
BLAST

Proteomic databases

PRIDEiP52955.

Expressioni

Tissue specificityi

Expressed in the dorsal part of the spinal cord and hindbrain and in presumptive myogenic cells in lateral regions of differentiating somites.1 Publication

Developmental stagei

Expressed in the developing central nervous system from 10.5 dpc to 16.5 dpc. Expressed in presumptive myogenic cells from 9.5 dpc until 16.5 dpc with highest levels at 10.5-11.5 dpc.1 Publication

Gene expression databases

BgeeiP52955.
CleanExiMM_LBX1.
GenevestigatoriP52955.

Interactioni

Subunit structurei

Interacts with SKOR1 which acts as a transcriptional corepressor.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Skor1Q8BX462EBI-604594,EBI-604451

Protein-protein interaction databases

BioGridi201117. 1 interaction.
IntActiP52955. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP52955.
SMRiP52955. Positions 124-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi219 – 2279Poly-Gly
Compositional biasi271 – 28212Asp/Glu-rich (highly acidic)Add
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG240187.
GeneTreeiENSGT00780000121835.
HOGENOMiHOG000007247.
HOVERGENiHBG006244.
InParanoidiP52955.
KOiK09353.
OMAiNSEATAG.
OrthoDBiEOG7X3QRB.
PhylomeDBiP52955.
TreeFamiTF325047.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52955-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKEDGKAA PGEERRRSPL DHLPPPANSN KPLTPFSIED ILNKPSVRRS
60 70 80 90 100
YSLCGAAHLL AAADKHAPGG LPLAGRALLS QTSPLCALEE LASKTFKGLE
110 120 130 140 150
VSVLQAAEGR DGMTIFGQRQ TPKKRRKSRT AFTNHQIYEL EKRFLYQKYL
160 170 180 190 200
SPADRDQIAQ QLGLTNAQVI TWFQNRRAKL KRDLEEMKAD VESAKKLGPS
210 220 230 240 250
GQMDIVALAE LEQNSEASGG GGGGGCGRAK SRPGSPALPP GAPQAPGGGP
260 270 280
LQLSPASPLT DQRASSQDCS EDEEDEEIDV DD
Length:282
Mass (Da):30,262
Last modified:November 14, 2006 - v2
Checksum:i3A4A80CD47B0228C
GO

Sequence cautioni

The sequence BAE25939.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 7240LTPFS…PGGLP → YAVQHRGHPQQAVRAEKLLA VWGGAPAGGRGQARAGRLA in CAA62343 (PubMed:8645601).CuratedAdd
BLAST
Sequence conflicti225 – 2251Missing in CAA62343 (PubMed:8645601).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90829 mRNA. Translation: CAA62343.1.
AB108497 mRNA. Translation: BAC75634.1.
BC119177 mRNA. Translation: AAI19178.1.
BC120586 mRNA. Translation: AAI20587.1.
AK144562 mRNA. Translation: BAE25939.1. Different initiation.
CCDSiCCDS29859.1.
RefSeqiNP_034821.2. NM_010691.5.
XP_006526785.1. XM_006526722.1.
UniGeneiMm.116815.

Genome annotation databases

EnsembliENSMUST00000099401; ENSMUSP00000096997; ENSMUSG00000025216.
GeneIDi16814.
KEGGimmu:16814.
UCSCiuc008hqv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90829 mRNA. Translation: CAA62343.1.
AB108497 mRNA. Translation: BAC75634.1.
BC119177 mRNA. Translation: AAI19178.1.
BC120586 mRNA. Translation: AAI20587.1.
AK144562 mRNA. Translation: BAE25939.1. Different initiation.
CCDSiCCDS29859.1.
RefSeqiNP_034821.2. NM_010691.5.
XP_006526785.1. XM_006526722.1.
UniGeneiMm.116815.

3D structure databases

ProteinModelPortaliP52955.
SMRiP52955. Positions 124-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201117. 1 interaction.
IntActiP52955. 2 interactions.

Proteomic databases

PRIDEiP52955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099401; ENSMUSP00000096997; ENSMUSG00000025216.
GeneIDi16814.
KEGGimmu:16814.
UCSCiuc008hqv.1. mouse.

Organism-specific databases

CTDi10660.
MGIiMGI:104867. Lbx1.

Phylogenomic databases

eggNOGiNOG240187.
GeneTreeiENSGT00780000121835.
HOGENOMiHOG000007247.
HOVERGENiHBG006244.
InParanoidiP52955.
KOiK09353.
OMAiNSEATAG.
OrthoDBiEOG7X3QRB.
PhylomeDBiP52955.
TreeFamiTF325047.

Miscellaneous databases

NextBioi290692.
PROiP52955.
SOURCEiSearch...

Gene expression databases

BgeeiP52955.
CleanExiMM_LBX1.
GenevestigatoriP52955.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Lbx1 and human LBX1 define a novel mammalian homeobox gene family related to the Drosophila lady bird genes."
    Jagla K., Dolle P., Mattei M.-G., Jagla T., Schuhbaur B., Dretzen G., Bellard F., Bellard M.
    Mech. Dev. 53:345-356(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/c.
  2. Jagla K.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Mouse homeobox gene Lbx1 in myogenesis."
    Kurose T., Endo T.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-282.
    Strain: C57BL/6J.
    Tissue: Medulla oblongata.
  6. "The role of Lbx1 in migration of muscle precursor cells."
    Brohmann H., Jagla K., Birchmeier C.
    Development 127:437-445(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "The homeodomain factor lbx1 distinguishes two major programs of neuronal differentiation in the dorsal spinal cord."
    Muller T., Brohmann H., Pierani A., Heppenstall P.A., Lewin G.R., Jessell T.M., Birchmeier C.
    Neuron 34:551-562(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Corl1, a novel neuronal lineage-specific transcriptional corepressor for the homeodomain transcription factor Lbx1."
    Mizuhara E., Nakatani T., Minaki Y., Sakamoto Y., Ono Y.
    J. Biol. Chem. 280:3645-3655(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKOR1.
  9. "Lbx1 and Tlx3 are opposing switches in determining GABAergic versus glutamatergic transmitter phenotypes."
    Cheng L., Samad O.A., Xu Y., Mizuguchi R., Luo P., Shirasawa S., Goulding M., Ma Q.
    Nat. Neurosci. 8:1510-1515(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiLBX1_MOUSE
AccessioniPrimary (citable) accession number: P52955
Secondary accession number(s): Q3UN09, Q80XA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 14, 2006
Last modified: February 4, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.