Reviewed, UniProtKB/Swiss-Prot P52948 (NUP98_HUMAN)

Names and origin

Protein names

Recommended name:
    Nuclear pore complex protein Nup98-Nup96
Cleaved into the following 2 chains:
    1- Recommended name:
            Nuclear pore complex protein Nup98
        Alternative name(s):
            Nucleoporin Nup98
            98 kDa nucleoporin
    2- Recommended name:
            Nuclear pore complex protein Nup96
        Alternative name(s):
            Nucleoporin Nup96
            96 kDa nucleoporin

Gene names

Name:	NUP98
Synonyms:	ADAR2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1729 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
Subunit structure	Nup98 interacts directly with Nup96. Nup96 is part of the Nup160 subcomplex in the nuclear pore which is composed of Nup160, Nup133, Nup107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and Nup153 to the nucleus. May interact with RAE1. Interacts with vesicular stomatitis virus protein M. Ref.11 Ref.12 Ref.14 Ref.27
Subcellular location	Nucleus › nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
Domain	Contains G-L-F-G repeats.
Post-translational modification	Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
Involvement in disease	A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23. A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1. A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1. A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1. A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
Sequence similarities	Belongs to the nucleoporin GLFG family. Contains 1 peptidase S59 domain.
Sequence caution	The sequence AAF19342.1 differs from that shown. Reason: Frameshift at position 1551.

Ontologies

Keywords
Biological process	Host-virus interaction Protein transport Translocation Transport mRNA transport
Cellular component	Membrane Nuclear pore complex Nucleus
Coding sequence diversity	Alternative splicing Chromosomal rearrangement Polymorphism
Domain	Repeat
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA replication Inferred from mutant phenotype. Source: UniProtKB interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW nuclear pore organization Non-traceable author statement. Source: UniProtKB protein import into nucleus, docking Non-traceable author statement. Source: UniProtKB
Cellular component	nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear pore Ref.3 Inferred from direct assay. Source: UniProtKB nucleoplasm Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB structural constituent of nuclear pore Non-traceable author statement. Source: UniProtKB transporter activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MOBKL1B	Q9H8S9	1	EBI-295727,EBI-748229
QRICH2	Q9H0J4	1	EBI-295727,EBI-1053637

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P52948-1)

Also known as: Nup98-Nup96 precursor;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P52948-2)

Also known as: Nup98-Nup96 precursor splice variant 1;

The sequence of this isoform differs from the canonical sequence as follows:
     393-409: Missing.
     1502-1576: RHYDLNQLLE...FVLLHIDNSG → S

Isoform 3 (identifier: P52948-3)

Also known as: Nup98-specific 1;

The sequence of this isoform differs from the canonical sequence as follows:
     932-937: SQSPEV → VEKKGQ
     938-1729: Missing.

Isoform 4 (identifier: P52948-4)

The sequence of this isoform differs from the canonical sequence as follows:
     393-409: Missing.
     932-937: SQSPEV → VEKKGQ
     938-1729: Missing.

Isoform 5 (identifier: P52948-5)

Also known as: Nup196; ADIR2;

The sequence of this isoform differs from the canonical sequence as follows:
     393-409: Missing.
     1710-1729: EHFNDSNIVQVMTWSSYTSK → DCSGNDLEQL...YLRELAVGSL

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 880

880

Nuclear pore complex protein Nup98

PRO_0000019929

Chain

881 – 1729

849

Nuclear pore complex protein Nup96

PRO_0000019930

Regions

Domain

738 – 880

143

Peptidase S59

Compositional bias

7 – 480

474

Gly/Thr-rich

Compositional bias

890 – 894

5

Poly-Glu

Sites

Site

531 – 532

2

Breakpoint for translocation to form NUP98-PHF23 oncogene

Amino acid modifications

Modified residue

608

1

Phosphoserine Ref.18 Ref.20 Ref.23 Ref.25

Modified residue

612

1

Phosphoserine Ref.18 Ref.20 Ref.23 Ref.25

Modified residue

623

1

Phosphoserine Ref.18 Ref.20 Ref.25 Ref.16 Ref.22

Modified residue

839

1

Phosphoserine Ref.18 Ref.25

Modified residue

888

1

Phosphoserine Ref.18 Ref.25 Ref.21 Ref.24 Ref.26

Modified residue

934

1

Phosphoserine Ref.25 Ref.24

Modified residue

1000

1

Phosphothreonine Ref.25

Modified residue

1023

1

Phosphoserine Ref.25

Modified residue

1028

1

Phosphoserine Ref.20 Ref.25

Modified residue

1043

1

Phosphoserine Ref.25

Modified residue

1060

1

Phosphoserine Ref.20 Ref.23 Ref.25

Modified residue

1064

1

Phosphoserine Ref.23

Modified residue

1070

1

Phosphothreonine Ref.25

Natural variations

Alternative sequence

393 – 409

17

Missing in isoform 2, isoform 4 and isoform 5.

VSP_003619

Alternative sequence

932 – 937

6

SQSPEV → VEKKGQ in isoform 3 and isoform 4.

VSP_007942

Alternative sequence

938 – 1729

792

Missing in isoform 3 and isoform 4.

VSP_007943

Alternative sequence

1502 – 1576

75

RHYDL…IDNSG → S in isoform 2.

VSP_007944

Alternative sequence

1710 – 1729

20

EHFND…SYTSK → DCSGNDLEQLHIKVTSLCSR IEQIQCYSAKDRLAQSDMAK RVANLLRVVLSLHHPPDRTS DSTPDPQRVPLRLLAPHIGR LPMPEDYAMDELRSLTQSYL RELAVGSL in isoform 5.

VSP_007945

Natural variant

1669

1

G → V in a breast cancer sample; somatic mutation. Ref.28

VAR_035859

Experimental info

Sequence conflict

318

1

L → S in AAH41136. Ref.6

Sequence conflict

376

1

S → G in AAH41136. Ref.6

Sequence conflict

756 – 757

2

EK → VF in AAD22395. Ref.3

Sequence conflict

756 – 757

2

EK → VF in AAD22396. Ref.3

Sequence conflict

1281

1

G → A in AAD22395. Ref.3

Sequence conflict

1281

1

G → A in AAD22396. Ref.3

Sequence conflict

1281

1

G → A in AAL56659. Ref.4

Sequence conflict

1534 – 1536

3

ALN → DLK in AAD22395. Ref.3

Sequence conflict

1594

1

E → D in AAF19342. Ref.7

Sequence conflict

1598

1

S → T in AAL56659. Ref.4

Sequence conflict

1639

1

K → N in AAF19342. Ref.7

Sequence conflict

1680

1

S → T in AAF19342. Ref.7

Secondary structure

1

.

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1729

Helix Strand Turn

Details...

Beta strand

741 – 745

5

Helix

747 – 753

7

Beta strand

761 – 769

9

Turn

770 – 772

3

Beta strand

773 – 777

5

Helix

788 – 791

4

Beta strand

792 – 795

4

Beta strand

798 – 804

7

Beta strand

819 – 823

5

Turn

831 – 833

3

Helix

840 – 846

7

Helix

848 – 858

11

Beta strand

862 – 867

6

Helix

868 – 870

3

Beta strand

872 – 878

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Nup98-Nup96 precursor) [UniParc]. Last modified August 15, 2003. Version 3. Checksum: A2037AAB8A1342B5	FASTA	1,729	187,790
	10 20 30 40 50 60 MFNKSFGTPF GGGTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG 70 80 90 100 110 120 GLFGTSSFSQ PATSTSTGFG FGTSTGTANT LFGTASTGTS LFSSQNNAFA QNKPTGFGNF 130 140 150 160 170 180 GTSTSSGGLF GTTNTTSNPF GSTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST 190 200 210 220 230 240 NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG AGTTTGLFGS SPATSSATGL 250 260 270 280 290 300 FSSSTTNSGF AYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTQNTGF 310 320 330 340 350 360 SFGNTSTIGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQTNTGFGAV 370 380 390 400 410 420 GSTLFGNNKL TTFGSSTTSA PSFGTTSGGL FGNKPTLTLG TNTNTSNFGF GTNTSGNSIF 430 440 450 460 470 480 GSKPAPGTLG TGLGAGFGTA LGAGQASLFG NNQPKIGGPL GTGAFGAPGF NTTTATLGFG 490 500 510 520 530 540 APQAPVALTD PNASAAQQAV LQQHINSLTY SPFGDSPLFR NPMSDPKKKE ERLKPTNPAA 550 560 570 580 590 600 QKALTTPTHY KLTPRPATRV RPKALQTTGT AKSHLFDGLD DDEPSLANGA FMPKKSIKKL 610 620 630 640 650 660 VLKNLNNSNL FSPVNRDSEN LASPSEYPEN GERFSFLSKP VDENHQQDGD EDSLVSHFYT 670 680 690 700 710 720 NPIAKPIPQT PESAGNKHSN SNSVDDTIVA LNMRAALRNG LEGSSEETSF HDESLQDDRE 730 740 750 760 770 780 EIENNSYHMH PAGIILTKVG YYTIPSMDDL AKITNEKGEC IVSDFTIGRK GYGSIYFEGD 790 800 810 820 830 840 VNLTNLNLDD IVHIRRKEVV VYLDDNQKPP VGEGLNRKAE VTLDGVWPTD KTSRCLIKSP 850 860 870 880 890 900 DRLADINYEG RLEAVSRKQG AQFKEYRPET GSWVFKVSHF SKYGLQDSDE EEEEHPSKTS 910 920 930 940 950 960 TKKLKTAPLP PASQTTPLQM ALNGKPAPPP QSQSPEVEQL GRVVELDSDM VDITQEPVLD 970 980 990 1000 1010 1020 TMLEESMPED QEPVSASTHI ASSLGINPHV LQIMKASLLT DEEDVDMALD QRFSRLPSKA 1030 1040 1050 1060 1070 1080 DTSQEICSPR LPISASHSSK TRSLVGGLLQ SKFTSGAFLS PSVSVQECRT PRAASLMNIP 1090 1100 1110 1120 1130 1140 STSSWSVPPP LTSVFTMPSP APEVPLKTVG TRRQLGLVPR EKSVTYGKGK LLMDMALFMG 1150 1160 1170 1180 1190 1200 RSFRVGWGPN WTLANSGEQL NGSHELENHQ IADSMEFGFL PNPVAVKPLT ESPFKVHLEK 1210 1220 1230 1240 1250 1260 LSLRQRKPDE DMKLYQTPLE LKLKHSTVHV DELCPLIVPN LGVAVIHDYA DWVKEASGDL 1270 1280 1290 1300 1310 1320 PEAQIVKHWS LTWTLCEALW GHLKELDSQL NEPREYIQIL ERRRAFSRWL SCTATPQIEE 1330 1340 1350 1360 1370 1380 EVSLTQKNSP VEAVFSYLTG KRISEACSLA QQSGDHRLAL LLSQFVGSQS VRELLTMQLV 1390 1400 1410 1420 1430 1440 DWHQLQADSF IQDERLRIFA LLAGKPVWQL SEKKQINVCS QLDWKRSLAI HLWYLLPPTA 1450 1460 1470 1480 1490 1500 SISRALSMYE EAFQNTSDSD RYACSPLPSY LEGSGCVIAE EQNSQTPLRD VCFHLLKLYS 1510 1520 1530 1540 1550 1560 DRHYDLNQLL EPRSITADPL DYRLSWHLWE VLRALNYTHL SAQCEGVLQA SYAGQLESEG 1570 1580 1590 1600 1610 1620 LWEWAIFVLL HIDNSGIREK AVRELLTRHC QLLETPESWA KETFLTQKLR VPAKWIHEAK 1630 1640 1650 1660 1670 1680 AVRAHMESDK HLEALCLFKA EHWNRCHKLI IRHLASDAII NENYDYLKGF LEDLAPPERS 1690 1700 1710 1720 SLIQDWETSG LVYLDYIRVI EMLRHIQQVE HFNDSNIVQV MTWSSYTSK
	Isoform 2 (Nup98-Nup96 precursor splice variant 1). Checksum: B7A54EDE0A21A98D	FASTA	1,638	177,409
	10 20 30 40 50 60 MFNKSFGTPF GGGTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG 70 80 90 100 110 120 GLFGTSSFSQ PATSTSTGFG FGTSTGTANT LFGTASTGTS LFSSQNNAFA QNKPTGFGNF 130 140 150 160 170 180 GTSTSSGGLF GTTNTTSNPF GSTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST 190 200 210 220 230 240 NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG AGTTTGLFGS SPATSSATGL 250 260 270 280 290 300 FSSSTTNSGF AYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTQNTGF 310 320 330 340 350 360 SFGNTSTIGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQTNTGFGAV 370 380 390 400 410 420 GSTLFGNNKL TTFGSSTTSA PSFGTTSGGL FGFGTNTSGN SIFGSKPAPG TLGTGLGAGF 430 440 450 460 470 480 GTALGAGQAS LFGNNQPKIG GPLGTGAFGA PGFNTTTATL GFGAPQAPVA LTDPNASAAQ 490 500 510 520 530 540 QAVLQQHINS LTYSPFGDSP LFRNPMSDPK KKEERLKPTN PAAQKALTTP THYKLTPRPA 550 560 570 580 590 600 TRVRPKALQT TGTAKSHLFD GLDDDEPSLA NGAFMPKKSI KKLVLKNLNN SNLFSPVNRD 610 620 630 640 650 660 SENLASPSEY PENGERFSFL SKPVDENHQQ DGDEDSLVSH FYTNPIAKPI PQTPESAGNK 670 680 690 700 710 720 HSNSNSVDDT IVALNMRAAL RNGLEGSSEE TSFHDESLQD DREEIENNSY HMHPAGIILT 730 740 750 760 770 780 KVGYYTIPSM DDLAKITNEK GECIVSDFTI GRKGYGSIYF EGDVNLTNLN LDDIVHIRRK 790 800 810 820 830 840 EVVVYLDDNQ KPPVGEGLNR KAEVTLDGVW PTDKTSRCLI KSPDRLADIN YEGRLEAVSR 850 860 870 880 890 900 KQGAQFKEYR PETGSWVFKV SHFSKYGLQD SDEEEEEHPS KTSTKKLKTA PLPPASQTTP 910 920 930 940 950 960 LQMALNGKPA PPPQSQSPEV EQLGRVVELD SDMVDITQEP VLDTMLEESM PEDQEPVSAS 970 980 990 1000 1010 1020 THIASSLGIN PHVLQIMKAS LLTDEEDVDM ALDQRFSRLP SKADTSQEIC SPRLPISASH 1030 1040 1050 1060 1070 1080 SSKTRSLVGG LLQSKFTSGA FLSPSVSVQE CRTPRAASLM NIPSTSSWSV PPPLTSVFTM 1090 1100 1110 1120 1130 1140 PSPAPEVPLK TVGTRRQLGL VPREKSVTYG KGKLLMDMAL FMGRSFRVGW GPNWTLANSG 1150 1160 1170 1180 1190 1200 EQLNGSHELE NHQIADSMEF GFLPNPVAVK PLTESPFKVH LEKLSLRQRK PDEDMKLYQT 1210 1220 1230 1240 1250 1260 PLELKLKHST VHVDELCPLI VPNLGVAVIH DYADWVKEAS GDLPEAQIVK HWSLTWTLCE 1270 1280 1290 1300 1310 1320 ALWGHLKELD SQLNEPREYI QILERRRAFS RWLSCTATPQ IEEEVSLTQK NSPVEAVFSY 1330 1340 1350 1360 1370 1380 LTGKRISEAC SLAQQSGDHR LALLLSQFVG SQSVRELLTM QLVDWHQLQA DSFIQDERLR 1390 1400 1410 1420 1430 1440 IFALLAGKPV WQLSEKKQIN VCSQLDWKRS LAIHLWYLLP PTASISRALS MYEEAFQNTS 1450 1460 1470 1480 1490 1500 DSDRYACSPL PSYLEGSGCV IAEEQNSQTP LRDVCFHLLK LYSDSIREKA VRELLTRHCQ 1510 1520 1530 1540 1550 1560 LLETPESWAK ETFLTQKLRV PAKWIHEAKA VRAHMESDKH LEALCLFKAE HWNRCHKLII 1570 1580 1590 1600 1610 1620 RHLASDAIIN ENYDYLKGFL EDLAPPERSS LIQDWETSGL VYLDYIRVIE MLRHIQQVEH 1630 FNDSNIVQVM TWSSYTSK
	Isoform 3 (Nup98-specific 1). Checksum: 2CB6CE36734F0B82	FASTA	937	97,836
	10 20 30 40 50 60 MFNKSFGTPF GGGTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG 70 80 90 100 110 120 GLFGTSSFSQ PATSTSTGFG FGTSTGTANT LFGTASTGTS LFSSQNNAFA QNKPTGFGNF 130 140 150 160 170 180 GTSTSSGGLF GTTNTTSNPF GSTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST 190 200 210 220 230 240 NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG AGTTTGLFGS SPATSSATGL 250 260 270 280 290 300 FSSSTTNSGF AYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTQNTGF 310 320 330 340 350 360 SFGNTSTIGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQTNTGFGAV 370 380 390 400 410 420 GSTLFGNNKL TTFGSSTTSA PSFGTTSGGL FGNKPTLTLG TNTNTSNFGF GTNTSGNSIF 430 440 450 460 470 480 GSKPAPGTLG TGLGAGFGTA LGAGQASLFG NNQPKIGGPL GTGAFGAPGF NTTTATLGFG 490 500 510 520 530 540 APQAPVALTD PNASAAQQAV LQQHINSLTY SPFGDSPLFR NPMSDPKKKE ERLKPTNPAA 550 560 570 580 590 600 QKALTTPTHY KLTPRPATRV RPKALQTTGT AKSHLFDGLD DDEPSLANGA FMPKKSIKKL 610 620 630 640 650 660 VLKNLNNSNL FSPVNRDSEN LASPSEYPEN GERFSFLSKP VDENHQQDGD EDSLVSHFYT 670 680 690 700 710 720 NPIAKPIPQT PESAGNKHSN SNSVDDTIVA LNMRAALRNG LEGSSEETSF HDESLQDDRE 730 740 750 760 770 780 EIENNSYHMH PAGIILTKVG YYTIPSMDDL AKITNEKGEC IVSDFTIGRK GYGSIYFEGD 790 800 810 820 830 840 VNLTNLNLDD IVHIRRKEVV VYLDDNQKPP VGEGLNRKAE VTLDGVWPTD KTSRCLIKSP 850 860 870 880 890 900 DRLADINYEG RLEAVSRKQG AQFKEYRPET GSWVFKVSHF SKYGLQDSDE EEEEHPSKTS 910 920 930 TKKLKTAPLP PASQTTPLQM ALNGKPAPPP QVEKKGQ
	Isoform 4. Checksum: 129DABAFB69253AD	FASTA	920	96,075
	10 20 30 40 50 60 MFNKSFGTPF GGGTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG 70 80 90 100 110 120 GLFGTSSFSQ PATSTSTGFG FGTSTGTANT LFGTASTGTS LFSSQNNAFA QNKPTGFGNF 130 140 150 160 170 180 GTSTSSGGLF GTTNTTSNPF GSTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST 190 200 210 220 230 240 NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG AGTTTGLFGS SPATSSATGL 250 260 270 280 290 300 FSSSTTNSGF AYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTQNTGF 310 320 330 340 350 360 SFGNTSTIGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQTNTGFGAV 370 380 390 400 410 420 GSTLFGNNKL TTFGSSTTSA PSFGTTSGGL FGFGTNTSGN SIFGSKPAPG TLGTGLGAGF 430 440 450 460 470 480 GTALGAGQAS LFGNNQPKIG GPLGTGAFGA PGFNTTTATL GFGAPQAPVA LTDPNASAAQ 490 500 510 520 530 540 QAVLQQHINS LTYSPFGDSP LFRNPMSDPK KKEERLKPTN PAAQKALTTP THYKLTPRPA 550 560 570 580 590 600 TRVRPKALQT TGTAKSHLFD GLDDDEPSLA NGAFMPKKSI KKLVLKNLNN SNLFSPVNRD 610 620 630 640 650 660 SENLASPSEY PENGERFSFL SKPVDENHQQ DGDEDSLVSH FYTNPIAKPI PQTPESAGNK 670 680 690 700 710 720 HSNSNSVDDT IVALNMRAAL RNGLEGSSEE TSFHDESLQD DREEIENNSY HMHPAGIILT 730 740 750 760 770 780 KVGYYTIPSM DDLAKITNEK GECIVSDFTI GRKGYGSIYF EGDVNLTNLN LDDIVHIRRK 790 800 810 820 830 840 EVVVYLDDNQ KPPVGEGLNR KAEVTLDGVW PTDKTSRCLI KSPDRLADIN YEGRLEAVSR 850 860 870 880 890 900 KQGAQFKEYR PETGSWVFKV SHFSKYGLQD SDEEEEEHPS KTSTKKLKTA PLPPASQTTP 910 920 LQMALNGKPA PPPQVEKKGQ
	Isoform 5 (Nup196) (ADIR2). Checksum: 4BCA483C2E9E1A8E	FASTA	1,800	195,817
	10 20 30 40 50 60 MFNKSFGTPF GGGTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG 70 80 90 100 110 120 GLFGTSSFSQ PATSTSTGFG FGTSTGTANT LFGTASTGTS LFSSQNNAFA QNKPTGFGNF 130 140 150 160 170 180 GTSTSSGGLF GTTNTTSNPF GSTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST 190 200 210 220 230 240 NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG AGTTTGLFGS SPATSSATGL 250 260 270 280 290 300 FSSSTTNSGF AYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTQNTGF 310 320 330 340 350 360 SFGNTSTIGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQTNTGFGAV 370 380 390 400 410 420 GSTLFGNNKL TTFGSSTTSA PSFGTTSGGL FGFGTNTSGN SIFGSKPAPG TLGTGLGAGF 430 440 450 460 470 480 GTALGAGQAS LFGNNQPKIG GPLGTGAFGA PGFNTTTATL GFGAPQAPVA LTDPNASAAQ 490 500 510 520 530 540 QAVLQQHINS LTYSPFGDSP LFRNPMSDPK KKEERLKPTN PAAQKALTTP THYKLTPRPA 550 560 570 580 590 600 TRVRPKALQT TGTAKSHLFD GLDDDEPSLA NGAFMPKKSI KKLVLKNLNN SNLFSPVNRD 610 620 630 640 650 660 SENLASPSEY PENGERFSFL SKPVDENHQQ DGDEDSLVSH FYTNPIAKPI PQTPESAGNK 670 680 690 700 710 720 HSNSNSVDDT IVALNMRAAL RNGLEGSSEE TSFHDESLQD DREEIENNSY HMHPAGIILT 730 740 750 760 770 780 KVGYYTIPSM DDLAKITNEK GECIVSDFTI GRKGYGSIYF EGDVNLTNLN LDDIVHIRRK 790 800 810 820 830 840 EVVVYLDDNQ KPPVGEGLNR KAEVTLDGVW PTDKTSRCLI KSPDRLADIN YEGRLEAVSR 850 860 870 880 890 900 KQGAQFKEYR PETGSWVFKV SHFSKYGLQD SDEEEEEHPS KTSTKKLKTA PLPPASQTTP 910 920 930 940 950 960 LQMALNGKPA PPPQSQSPEV EQLGRVVELD SDMVDITQEP VLDTMLEESM PEDQEPVSAS 970 980 990 1000 1010 1020 THIASSLGIN PHVLQIMKAS LLTDEEDVDM ALDQRFSRLP SKADTSQEIC SPRLPISASH 1030 1040 1050 1060 1070 1080 SSKTRSLVGG LLQSKFTSGA FLSPSVSVQE CRTPRAASLM NIPSTSSWSV PPPLTSVFTM 1090 1100 1110 1120 1130 1140 PSPAPEVPLK TVGTRRQLGL VPREKSVTYG KGKLLMDMAL FMGRSFRVGW GPNWTLANSG 1150 1160 1170 1180 1190 1200 EQLNGSHELE NHQIADSMEF GFLPNPVAVK PLTESPFKVH LEKLSLRQRK PDEDMKLYQT 1210 1220 1230 1240 1250 1260 PLELKLKHST VHVDELCPLI VPNLGVAVIH DYADWVKEAS GDLPEAQIVK HWSLTWTLCE 1270 1280 1290 1300 1310 1320 ALWGHLKELD SQLNEPREYI QILERRRAFS RWLSCTATPQ IEEEVSLTQK NSPVEAVFSY 1330 1340 1350 1360 1370 1380 LTGKRISEAC SLAQQSGDHR LALLLSQFVG SQSVRELLTM QLVDWHQLQA DSFIQDERLR 1390 1400 1410 1420 1430 1440 IFALLAGKPV WQLSEKKQIN VCSQLDWKRS LAIHLWYLLP PTASISRALS MYEEAFQNTS 1450 1460 1470 1480 1490 1500 DSDRYACSPL PSYLEGSGCV IAEEQNSQTP LRDVCFHLLK LYSDRHYDLN QLLEPRSITA 1510 1520 1530 1540 1550 1560 DPLDYRLSWH LWEVLRALNY THLSAQCEGV LQASYAGQLE SEGLWEWAIF VLLHIDNSGI 1570 1580 1590 1600 1610 1620 REKAVRELLT RHCQLLETPE SWAKETFLTQ KLRVPAKWIH EAKAVRAHME SDKHLEALCL 1630 1640 1650 1660 1670 1680 FKAEHWNRCH KLIIRHLASD AIINENYDYL KGFLEDLAPP ERSSLIQDWE TSGLVYLDYI 1690 1700 1710 1720 1730 1740 RVIEMLRHIQ QVDCSGNDLE QLHIKVTSLC SRIEQIQCYS AKDRLAQSDM AKRVANLLRV 1750 1760 1770 1780 1790 1800 VLSLHHPPDR TSDSTPDPQR VPLRLLAPHI GRLPMPEDYA MDELRSLTQS YLRELAVGSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The t(7;11)(p15;p15) translocation in acute myeloid leukaemia fuses the genes for nucleoporin NUP98 and class I homeoprotein HOXA9." Borrow J., Shearman A.M., Stanton V.P., Becher R., Collins T., Williams A.J., Dube I., Katz F., Kwong Y.L., Morris C., Ohyashiki K., Toyama K., Rowley J., Housman D.E. Nat. Genet. 12:159-167(1996) [PubMed: 8563754] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), CHROMOSOMAL TRANSLOCATION WITH HOXA9.
[2]	"Molecular analysis of the chromosomal breakpoints and identification of the repetitive sequences near the breakpoints of NUP98 in therapy-related leukemia with inv(11)(p15q22)." Arai Y., Kaneko Y., Kubo T., Arai K., Hosoda F., Ohki M. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	"A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96." Fontoura B.M.A., Blobel G., Matunis M.J. J. Cell Biol. 144:1097-1112(1999) [PubMed: 10087256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF N-TERMINUS OF NUP96, PROTEIN SEQUENCE OF 1648-1664.
[4]	"An alternative splice form of NUP98 encodes a 196kDa NUP196 isoform." Borrow J., Housman D.E. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
[5]	"A novel NUP98-PHF23 fusion resulting from a cryptic translocation t(11;17)(p15;p13) in acute myeloid leukemia." Reader J.C., Meekins J.S., Gojo I., Ning Y. Leukemia 21:842-844(2007) [PubMed: 17287853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHROMOSOMAL REARRANGEMENT. Tissue: Peripheral blood.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Brain, Lung and Testis.
[7]	Xu Y.H., Guo B.C., Yu Y.L. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1176-1729 (ISOFORM 5). Tissue: Liver.
[8]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1212-1729 (ISOFORM 5).
[9]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1227-1729 (ISOFORM 5). Tissue: Testis.
[10]	"The t(11;20)(p15;q11) chromosomal translocation associated with therapy-related myelodysplastic syndrome results in an NUP98-TOP1 fusion." Ahuja H.G., Felix C.A., Aplan P.D. Blood 94:3258-3261(1999) [PubMed: 10556215] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION WITH TOP1.
[11]	"RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains." Pritchard C.E., Fornerod M., Kasper L.H., van Deursen J.M. J. Cell Biol. 145:237-254(1999) [PubMed: 10209021] [Abstract] Cited for: INTERACTION WITH RAE1.
[12]	"Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98." von Kobbe C., van Deursen J.M., Rodrigues J.P., Sitterlin D., Bachi A., Wu X., Wilm M., Carmo-Fonseca M., Izaurralde E. Mol. Cell 6:1243-1252(2000) [PubMed: 11106761] [Abstract] Cited for: INTERACTION WITH VESICULAR STOMATITIS VIRUS PROTEIN M.
[13]	"A novel gene, NSD1, is fused to NUP98 in the t(5;11)(q35;p15.5) in de novo childhood acute myeloid leukemia." Jaju R.J., Fidler C., Haas O.A., Strickson A.J., Watkins F., Clark K., Cross N.C., Cheng J.F., Aplan P.D., Kearney L., Boultwood J., Wainscoat J.S. Blood 98:1264-1267(2001) [PubMed: 11493482] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION WITH NSD1.
[14]	"Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export." Vasu S., Shah S., Orjalo A., Park M., Fischer W.H., Forbes D.J. J. Cell Biol. 155:339-354(2001) [PubMed: 11684705] [Abstract] Cited for: SUBUNIT.
[15]	"NUP98 is fused to the NSD3 gene in acute myeloid leukemia associated with t(8;11)(p11.2;p15)." Rosati R., La Starza R., Veronese A., Aventin A., Schwienbacher C., Vallespi T., Negrini M., Martelli M.F., Mecucci C. Blood 99:3857-3860(2002) [PubMed: 11986249] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION WITH WHSC1L1.
[16]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-623, MASS SPECTROMETRY. Tissue: Epithelium.
[17]	"t(9;11)(p22;p15) with NUP98-LEDGF fusion gene in pediatric acute myeloid leukemia." Morerio C., Acquila M., Rosanda C., Rapella A., Tassano E., Micalizzi C., Panarello C. Leuk. Res. 29:467-470(2005) [PubMed: 15725483] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION WITH PSIP1/LEDGF.
[18]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-608; SER-612; SER-623; SER-839 AND SER-888, MASS SPECTROMETRY. Tissue: Epithelium.
[19]	"NUP98 rearrangements in hematopoietic malignancies: a study of the Groupe Francophone de Cytogenetique Hematologique." Romana S.P., Radford-Weiss I., Ben Abdelali R., Schluth C., Petit A., Dastugue N., Talmant P., Bilhou-Nabera C., Mugneret F., Lafage-Pochitaloff M., Mozziconacci M.-J., Andrieu J., Lai J.-L., Terre C., Rack K., Cornillet-Lefebvre P., Luquet I., Nadal N. , Nguyen-Khac F., Perot C., Van den Akker J., Fert-Ferrer S., Cabrol C., Charrin C., Tigaud I., Poirel H., Vekemans M., Bernard O.A., Berger R. Leukemia 20:696-706(2006) [PubMed: 16467868] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION WITH LNP1.
[20]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-608; SER-612; SER-623; SER-1028 AND SER-1060, MASS SPECTROMETRY. Tissue: Epithelium.
[21]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-888, MASS SPECTROMETRY. Tissue: Epithelium.
[22]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-623, MASS SPECTROMETRY.
[23]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-608; SER-612; SER-1060 AND SER-1064, MASS SPECTROMETRY.
[24]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-888 AND SER-934, MASS SPECTROMETRY.
[25]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-608; SER-612; SER-623; SER-839; SER-888; SER-934; THR-1000; SER-1023; SER-1028; SER-1043; SER-1060 AND THR-1070, MASS SPECTROMETRY.
[26]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-888, MASS SPECTROMETRY. Tissue: Liver.
[27]	"The three-dimensional structure of the autoproteolytic, nuclear pore-targeting domain of the human nucleoporin Nup98." Hodel A.E., Hodel M.R., Griffis E.R., Hennig K.A., Ratner G.A., Xu S., Powers M.A. Mol. Cell 10:347-358(2002) [PubMed: 12191480] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 710-870 OF NUP98, INTERACTION WITH NUP96.
[28]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-1669.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

U41815 mRNA. Translation: AAC50366.1.
AB040538 mRNA. Translation: BAB18537.1.
AF071076 mRNA. Translation: AAD22395.1.
AF071077 mRNA. Translation: AAD22396.1.
AF231130 mRNA. Translation: AAL56659.1.
BC041136 mRNA. Translation: AAH41136.1.
BC012906 mRNA. Translation: AAH12906.1. Different initiation.
AF116074 mRNA. Translation: AAF19342.1. Sequence problems.
BT007349 mRNA. Translation: AAP36013.1.
AL133601 mRNA. Translation: CAB63736.1.
AL137613 mRNA. Translation: CAB70842.1.

IPI

IPI00006038.
IPI00216688.
IPI00337395.
IPI00337396.
IPI00337397.

PIR

T43443.

RefSeq

NP_005378.4.
NP_057404.2.
NP_624357.1.
NP_624358.2.

UniGene

Hs.524750

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KO6	X-ray	3.00	A/C	695-880	[»]
			B/D	881-931	[»]
2Q5X	X-ray	1.90	A	733-887	[»]
2Q5Y	X-ray	2.30	A/C	729-880	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P52948. 6 interactions.

PTM databases

PhosphoSite

P52948.

Proteomic databases

PRIDE

P52948.

Genome annotation databases

Ensembl

ENSG00000110713. Homo sapiens. [Contig view]

GeneID

4928.

KEGG

hsa:4928.

Organism-specific databases

GeneCards

GC11M003652.

H-InvDB

HIX0009377.

HGNC

HGNC:8068. NUP98.

MIM

601021. gene.

PharmGKB

PA31856.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P52948.

OMA

P52948. SLTESPF.

Enzyme and pathway databases

Reactome

REACT_152. Cell Cycle, Mitotic.
REACT_6185. HIV Infection.

Gene expression databases

ArrayExpress

P52948.

Bgee

P52948.

GermOnline

ENSG00000110713. Homo sapiens.

Family and domain databases

InterPro

IPR007230. Peptidase_S59.
[Graphical view]

Gene3D

G3DSA:3.30.1610.10. Peptidase_S59. 1 hit.

Pfam

PF04096. Nucleoporin2. 1 hit.
[Graphical view]

PROSITE

PS51434. NUP_C. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

18979.

PMAP-CutDB

P52948.

SOURCE

Search...

Entry information

Entry name

NUP98_HUMAN

Accession

Primary (citable) accession number: P52948
Secondary accession number(s): Q8IUT2 , Q8WYB0, Q96E54, Q9H3Q4, Q9NT02, Q9UF57, Q9UHX0, Q9Y6J4, Q9Y6J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	August 15, 2003
Last modified:	June 16, 2009
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)