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P52927

- HMGA2_MOUSE

UniProt

P52927 - HMGA2_MOUSE

Protein

High mobility group protein HMGI-C

Gene

Hmga2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi24 – 3411A.T hook 1Add
    BLAST
    DNA bindingi44 – 5411A.T hook 2Add
    BLAST
    DNA bindingi71 – 8212A.T hook 3Add
    BLAST

    GO - Molecular functioni

    1. 5'-deoxyribose-5-phosphate lyase activity Source: UniProtKB
    2. AT DNA binding Source: UniProtKB
    3. C2H2 zinc finger domain binding Source: UniProtKB
    4. cAMP response element binding Source: UniProtKB
    5. core promoter binding Source: UniProtKB
    6. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB
    7. DNA binding, bending Source: UniProtKB
    8. DNA-dependent protein kinase activity Source: UniProtKB
    9. MH1 domain binding Source: UniProtKB
    10. MH2 domain binding Source: UniProtKB
    11. nucleosomal DNA binding Source: UniProtKB
    12. protein binding Source: IntAct
    13. protein binding transcription factor activity Source: Ensembl
    14. regulatory region DNA binding Source: UniProtKB
    15. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: UniProtKB
    16. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI

    GO - Biological processi

    1. adipose tissue development Source: MGI
    2. adrenal gland development Source: MGI
    3. base-excision repair Source: UniProtKB
    4. cell proliferation Source: MGI
    5. cell proliferation in forebrain Source: MGI
    6. chondrocyte differentiation Source: UniProtKB
    7. chondrocyte proliferation Source: UniProtKB
    8. chromosome breakage Source: UniProtKB
    9. chromosome condensation Source: UniProtKB-KW
    10. DNA catabolic process, endonucleolytic Source: GOC
    11. DNA damage response, detection of DNA damage Source: UniProtKB
    12. endodermal cell differentiation Source: UniProtKB
    13. epithelial to mesenchymal transition Source: UniProtKB
    14. fat cell differentiation Source: UniProtKB
    15. fat pad development Source: MGI
    16. heterochromatin assembly Source: UniProtKB
    17. histone H2A-S139 phosphorylation Source: UniProtKB
    18. male gonad development Source: MGI
    19. meiotic nuclear division Source: UniProtKB-KW
    20. mesenchymal cell differentiation Source: UniProtKB
    21. mesodermal cell differentiation Source: UniProtKB
    22. mesodermal-endodermal cell signaling Source: UniProtKB
    23. mitotic G2 DNA damage checkpoint Source: UniProtKB
    24. mitotic nuclear division Source: UniProtKB-KW
    25. negative regulation by host of viral transcription Source: UniProtKB
    26. negative regulation of apoptotic process Source: UniProtKB
    27. negative regulation of astrocyte differentiation Source: MGI
    28. negative regulation of DNA binding Source: UniProtKB
    29. negative regulation of double-strand break repair via nonhomologous end joining Source: UniProtKB
    30. negative regulation of intracellular steroid hormone receptor signaling pathway Source: Ensembl
    31. negative regulation of JAK-STAT cascade Source: Ensembl
    32. negative regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: UniProtKB
    33. negative regulation of transcription, DNA-templated Source: UniProtKB
    34. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    35. oncogene-induced cell senescence Source: UniProtKB
    36. pituitary gland development Source: MGI
    37. positive regulation of apoptotic process Source: UniProtKB
    38. positive regulation of cell cycle arrest Source: UniProtKB
    39. positive regulation of cell proliferation Source: MGI
    40. positive regulation of cellular response to X-ray Source: UniProtKB
    41. positive regulation of cellular senescence Source: UniProtKB
    42. positive regulation of gene expression Source: UniProtKB
    43. positive regulation of multicellular organism growth Source: MGI
    44. positive regulation of response to DNA damage stimulus Source: UniProtKB
    45. positive regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
    46. positive regulation of stem cell proliferation Source: UniProtKB
    47. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    48. positive regulation of transcription regulatory region DNA binding Source: UniProtKB
    49. regulation of cell cycle process Source: UniProtKB
    50. regulation of cellular response to drug Source: UniProtKB
    51. regulation of growth hormone secretion Source: MGI
    52. regulation of peptide hormone secretion Source: MGI
    53. regulation of stem cell maintenance Source: UniProtKB
    54. regulation of transcription, DNA-templated Source: UniProtKB
    55. response to virus Source: Ensembl
    56. senescence-associated heterochromatin focus assembly Source: UniProtKB
    57. signal transduction Source: Ensembl
    58. somatic stem cell maintenance Source: MGI
    59. spermatogenesis Source: MGI

    Keywords - Biological processi

    Cell cycle, Cell division, DNA condensation, Growth regulation, Meiosis, Mitosis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_209428. Formation of Senescence-Associated Heterochromatin Foci (SAHF).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High mobility group protein HMGI-C
    Alternative name(s):
    High mobility group AT-hook protein 2
    Gene namesi
    Name:Hmga2
    Synonyms:Hmgic
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:101761. Hmga2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nuclear chromosome Source: MGI
    2. nucleus Source: UniProtKB
    3. protein-DNA complex Source: UniProtKB
    4. senescence-associated heterochromatin focus Source: UniProtKB
    5. SMAD protein complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 108107High mobility group protein HMGI-CPRO_0000206712Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei40 – 401PhosphothreonineBy similarity
    Modified residuei44 – 441PhosphoserineBy similarity
    Modified residuei104 – 1041Phosphoserine2 Publications

    Post-translational modificationi

    Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2.2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP52927.
    PRIDEiP52927.

    PTM databases

    PhosphoSiteiP52927.

    Expressioni

    Tissue specificityi

    Expressed in mitotic spermatogonia, meiotic spermatocytes, and postmeiotic round spermatids (at protein level).1 Publication

    Developmental stagei

    Expressed predominantly during embryogenesis.

    Gene expression databases

    BgeeiP52927.
    CleanExiMM_HMGA2.
    GenevestigatoriP52927.

    Interactioni

    Subunit structurei

    Interacts with E4F1 By similarity. Interacts with NEK2.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RB1P064005EBI-912574,EBI-491274From a different organism.

    Protein-protein interaction databases

    BioGridi200341. 7 interactions.
    IntActiP52927. 5 interactions.
    MINTiMINT-1847926.
    STRINGi10090.ENSMUSP00000072556.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni44 – 6320Interaction with E4F1By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HMGA family.Curated
    Contains 3 A.T hook DNA-binding domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG295015.
    GeneTreeiENSGT00730000111387.
    HOGENOMiHOG000076308.
    HOVERGENiHBG051913.
    InParanoidiP52927.
    KOiK09283.
    OMAiNSIKRSH.
    OrthoDBiEOG7QRQXW.
    PhylomeDBiP52927.
    TreeFamiTF351623.

    Family and domain databases

    InterProiIPR020478. AT_hook-like.
    IPR017956. AT_hook_DNA-bd_motif.
    IPR000116. HMGI/HMGY.
    [Graphical view]
    PfamiPF02178. AT_hook. 3 hits.
    [Graphical view]
    PRINTSiPR00929. ATHOOK.
    PR00930. HIGHMOBLTYIY.
    SMARTiSM00384. AT_hook. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52927-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSARGEGAGQ PSTSAQGQPA APVPQKRGRG RPRKQQQEPT CEPSPKRPRG    50
    RPKGSKNKSP SKAAQKKAET IGEKRPRGRP RKWPQQVVQK KPAQETEETS 100
    SQESAEED 108
    Length:108
    Mass (Da):11,819
    Last modified:October 1, 1996 - v1
    Checksum:i40A3E38F62268C09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L41621
    , L41617, L41618, L41619, L41620 Genomic DNA. Translation: AAC69244.1.
    X58380 mRNA. Translation: CAA41270.1.
    X99915
    , X99916, X99917, X99918, X99919 Genomic DNA. Translation: CAA68187.1.
    AK028346 mRNA. Translation: BAC25896.1.
    AK142059 mRNA. Translation: BAE24928.1.
    BC052158 mRNA. Translation: AAH52158.1.
    BC085085 mRNA. Translation: AAH85085.1.
    CCDSiCCDS36073.1.
    PIRiJC4575.
    RefSeqiNP_034571.1. NM_010441.2.
    UniGeneiMm.157190.

    Genome annotation databases

    EnsembliENSMUST00000072777; ENSMUSP00000072556; ENSMUSG00000056758.
    GeneIDi15364.
    KEGGimmu:15364.
    UCSCiuc007hfb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L41621
    , L41617 , L41618 , L41619 , L41620 Genomic DNA. Translation: AAC69244.1 .
    X58380 mRNA. Translation: CAA41270.1 .
    X99915
    , X99916 , X99917 , X99918 , X99919 Genomic DNA. Translation: CAA68187.1 .
    AK028346 mRNA. Translation: BAC25896.1 .
    AK142059 mRNA. Translation: BAE24928.1 .
    BC052158 mRNA. Translation: AAH52158.1 .
    BC085085 mRNA. Translation: AAH85085.1 .
    CCDSi CCDS36073.1.
    PIRi JC4575.
    RefSeqi NP_034571.1. NM_010441.2.
    UniGenei Mm.157190.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200341. 7 interactions.
    IntActi P52927. 5 interactions.
    MINTi MINT-1847926.
    STRINGi 10090.ENSMUSP00000072556.

    PTM databases

    PhosphoSitei P52927.

    Proteomic databases

    PaxDbi P52927.
    PRIDEi P52927.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000072777 ; ENSMUSP00000072556 ; ENSMUSG00000056758 .
    GeneIDi 15364.
    KEGGi mmu:15364.
    UCSCi uc007hfb.1. mouse.

    Organism-specific databases

    CTDi 8091.
    MGIi MGI:101761. Hmga2.

    Phylogenomic databases

    eggNOGi NOG295015.
    GeneTreei ENSGT00730000111387.
    HOGENOMi HOG000076308.
    HOVERGENi HBG051913.
    InParanoidi P52927.
    KOi K09283.
    OMAi NSIKRSH.
    OrthoDBi EOG7QRQXW.
    PhylomeDBi P52927.
    TreeFami TF351623.

    Enzyme and pathway databases

    Reactomei REACT_209428. Formation of Senescence-Associated Heterochromatin Foci (SAHF).

    Miscellaneous databases

    ChiTaRSi HMGA2. mouse.
    NextBioi 287988.
    PROi P52927.
    SOURCEi Search...

    Gene expression databases

    Bgeei P52927.
    CleanExi MM_HMGA2.
    Genevestigatori P52927.

    Family and domain databases

    InterProi IPR020478. AT_hook-like.
    IPR017956. AT_hook_DNA-bd_motif.
    IPR000116. HMGI/HMGY.
    [Graphical view ]
    Pfami PF02178. AT_hook. 3 hits.
    [Graphical view ]
    PRINTSi PR00929. ATHOOK.
    PR00930. HIGHMOBLTYIY.
    SMARTi SM00384. AT_hook. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of the HMGI-C phosphoprotein, a nuclear protein associated with neoplastic and undifferentiated phenotypes."
      Manfioletti G., Giancotti V., Bandiera A., Buratti E., Sautiere P., Cary P., Crane-Robinson C., Coles B., Goodwin G.H.
      Nucleic Acids Res. 19:6793-6797(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation and characterization of the gene coding for murine high-mobility-group protein HMGI-C."
      Manfioletti G., Rustighi A., Mantovani F., Goodwin G.H., Giancotti V.
      Gene 167:249-253(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Genomic structure and expression of the murine Hmgi-c gene."
      Zhou X., Benson K.F., Przybysz K., Liu J., Hou Y., Cherath L., Chada K.
      Nucleic Acids Res. 24:4071-4077(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo and Limb.
    6. "Phosphorylation of high-mobility group protein A2 by Nek2 kinase during the first meiotic division in mouse spermatocytes."
      Di Agostino S., Fedele M., Chieffi P., Fusco A., Rossi P., Geremia R., Sette C.
      Mol. Biol. Cell 15:1224-1232(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH NEK2, TISSUE SPECIFICITY.
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiHMGA2_MOUSE
    AccessioniPrimary (citable) accession number: P52927
    Secondary accession number(s): Q3UQW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3