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Protein

High mobility group protein HMGI-C

Gene

Hmga2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (PubMed:27446912).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi24 – 34A.T hook 1Add BLAST11
DNA bindingi44 – 54A.T hook 2Add BLAST11
DNA bindingi71 – 82A.T hook 3Add BLAST12

GO - Molecular functioni

GO - Biological processi

  • adipose tissue development Source: MGI
  • adrenal gland development Source: MGI
  • base-excision repair Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • cell proliferation Source: MGI
  • cell proliferation in forebrain Source: MGI
  • chondrocyte differentiation Source: UniProtKB
  • chondrocyte proliferation Source: UniProtKB
  • chromosome breakage Source: UniProtKB
  • chromosome condensation Source: UniProtKB-KW
  • DNA damage response, detection of DNA damage Source: UniProtKB
  • endodermal cell differentiation Source: UniProtKB
  • epithelial to mesenchymal transition Source: UniProtKB
  • epithelial tube branching involved in lung morphogenesis Source: MGI
  • fat cell differentiation Source: UniProtKB
  • fat pad development Source: MGI
  • heterochromatin assembly Source: UniProtKB
  • histone H2A-S139 phosphorylation Source: UniProtKB
  • lung epithelium development Source: MGI
  • male gonad development Source: MGI
  • meiotic cell cycle Source: UniProtKB-KW
  • mesenchymal cell differentiation Source: UniProtKB
  • mesodermal cell differentiation Source: UniProtKB
  • mesodermal-endodermal cell signaling Source: UniProtKB
  • mitotic G2 DNA damage checkpoint Source: UniProtKB
  • negative regulation by host of viral transcription Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of astrocyte differentiation Source: MGI
  • negative regulation of DNA binding Source: UniProtKB
  • negative regulation of double-strand break repair via nonhomologous end joining Source: UniProtKB
  • negative regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • negative regulation of Wnt signaling pathway Source: MGI
  • oncogene-induced cell senescence Source: UniProtKB
  • pituitary gland development Source: MGI
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of cell cycle arrest Source: UniProtKB
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of cellular response to X-ray Source: UniProtKB
  • positive regulation of cellular senescence Source: UniProtKB
  • positive regulation of epithelial cell proliferation involved in lung morphogenesis Source: MGI
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of multicellular organism growth Source: MGI
  • positive regulation of response to DNA damage stimulus Source: UniProtKB
  • positive regulation of stem cell proliferation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription regulatory region DNA binding Source: UniProtKB
  • regulation of cell cycle process Source: UniProtKB
  • regulation of cellular response to drug Source: UniProtKB
  • regulation of growth hormone secretion Source: MGI
  • regulation of peptide hormone secretion Source: MGI
  • regulation of stem cell population maintenance Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • senescence-associated heterochromatin focus assembly Source: UniProtKB
  • somatic stem cell population maintenance Source: MGI
  • spermatogenesis Source: MGI

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division, DNA condensation, Growth regulation, Meiosis, Mitosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2559584. Formation of Senescence-Associated Heterochromatin Foci (SAHF).

Names & Taxonomyi

Protein namesi
Recommended name:
High mobility group protein HMGI-C
Alternative name(s):
High mobility group AT-hook protein 2
Gene namesi
Name:Hmga2
Synonyms:Hmgic
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:101761. Hmga2.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromosome Source: MGI
  • nucleus Source: MGI
  • protein-DNA complex Source: UniProtKB
  • senescence-associated heterochromatin focus Source: UniProtKB
  • SMAD protein complex Source: UniProtKB

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002067122 – 108High mobility group protein HMGI-CAdd BLAST107

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei40PhosphothreonineBy similarity1
Modified residuei44PhosphoserineBy similarity1
Modified residuei100PhosphoserineCombined sources1
Modified residuei104PhosphoserineCombined sources1

Post-translational modificationi

Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP52927.
PaxDbiP52927.
PeptideAtlasiP52927.
PRIDEiP52927.

PTM databases

iPTMnetiP52927.
PhosphoSitePlusiP52927.

Expressioni

Tissue specificityi

Expressed in mitotic spermatogonia, meiotic spermatocytes, and postmeiotic round spermatids (at protein level) (PubMed:14668482). Expressed in embronic myogenic progenitor cells (PubMed:27446912).2 Publications

Developmental stagei

Expressed predominantly during embryogenesis. In myogenic progenitor cells, expressed during early myogenic development (E11.5) to be gradually down-regulated during the fetal stages (E17.5) (PubMed:27446912).1 Publication

Gene expression databases

BgeeiENSMUSG00000056758.
CleanExiMM_HMGA2.
ExpressionAtlasiP52927. baseline and differential.
GenevisibleiP52927. MM.

Interactioni

Subunit structurei

Interacts with E4F1 (By similarity). Interacts with NEK2 (PubMed:14668482).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RB1P064005EBI-912574,EBI-491274From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200341. 7 interactors.
IntActiP52927. 476 interactors.
MINTiMINT-1847926.
STRINGi10090.ENSMUSP00000072556.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni44 – 63Interaction with E4F1By similarityAdd BLAST20

Sequence similaritiesi

Belongs to the HMGA family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IZGR. Eukaryota.
ENOG410YWK3. LUCA.
GeneTreeiENSGT00730000111387.
HOGENOMiHOG000076308.
HOVERGENiHBG051913.
InParanoidiP52927.
KOiK09283.
OMAiPRKWPRK.
PhylomeDBiP52927.
TreeFamiTF351623.

Family and domain databases

InterProiView protein in InterPro
IPR017956. AT_hook_DNA-bd_motif.
IPR000116. HMGA.
PfamiView protein in Pfam
PF02178. AT_hook. 3 hits.
PRINTSiPR00929. ATHOOK.
PR00930. HIGHMOBLTYIY.
SMARTiView protein in SMART
SM00384. AT_hook. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARGEGAGQ PSTSAQGQPA APVPQKRGRG RPRKQQQEPT CEPSPKRPRG
60 70 80 90 100
RPKGSKNKSP SKAAQKKAET IGEKRPRGRP RKWPQQVVQK KPAQETEETS

SQESAEED
Length:108
Mass (Da):11,819
Last modified:October 1, 1996 - v1
Checksum:i40A3E38F62268C09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41621
, L41617, L41618, L41619, L41620 Genomic DNA. Translation: AAC69244.1.
X58380 mRNA. Translation: CAA41270.1.
X99915
, X99916, X99917, X99918, X99919 Genomic DNA. Translation: CAA68187.1.
AK028346 mRNA. Translation: BAC25896.1.
AK142059 mRNA. Translation: BAE24928.1.
BC052158 mRNA. Translation: AAH52158.1.
BC085085 mRNA. Translation: AAH85085.1.
CCDSiCCDS36073.1.
PIRiJC4575.
RefSeqiNP_001334099.1. NM_001347170.1.
NP_034571.1. NM_010441.3.
UniGeneiMm.157190.

Genome annotation databases

EnsembliENSMUST00000072777; ENSMUSP00000072556; ENSMUSG00000056758.
GeneIDi15364.
KEGGimmu:15364.
UCSCiuc007hfb.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHMGA2_MOUSE
AccessioniPrimary (citable) accession number: P52927
Secondary accession number(s): Q3UQW0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families