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Protein

High mobility group protein HMGI-C

Gene

Hmga2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi24 – 3411A.T hook 1Add
BLAST
DNA bindingi44 – 5411A.T hook 2Add
BLAST
DNA bindingi71 – 8212A.T hook 3Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA condensation, Growth regulation, Meiosis, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_341685. Formation of Senescence-Associated Heterochromatin Foci (SAHF).

Names & Taxonomyi

Protein namesi
Recommended name:
High mobility group protein HMGI-C
Alternative name(s):
High mobility group AT-hook protein 2
Gene namesi
Name:Hmga2
Synonyms:Hmgic
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:101761. Hmga2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 108107High mobility group protein HMGI-CPRO_0000206712Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei40 – 401PhosphothreonineBy similarity
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei104 – 1041Phosphoserine1 Publication

Post-translational modificationi

Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP52927.
PaxDbiP52927.
PRIDEiP52927.

PTM databases

PhosphoSiteiP52927.

Expressioni

Tissue specificityi

Expressed in mitotic spermatogonia, meiotic spermatocytes, and postmeiotic round spermatids (at protein level).1 Publication

Developmental stagei

Expressed predominantly during embryogenesis.

Gene expression databases

BgeeiP52927.
CleanExiMM_HMGA2.
ExpressionAtlasiP52927. baseline and differential.
GenevisibleiP52927. MM.

Interactioni

Subunit structurei

Interacts with E4F1 (By similarity). Interacts with NEK2.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RB1P064005EBI-912574,EBI-491274From a different organism.

Protein-protein interaction databases

BioGridi200341. 7 interactions.
IntActiP52927. 476 interactions.
MINTiMINT-1847926.
STRINGi10090.ENSMUSP00000072556.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni44 – 6320Interaction with E4F1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the HMGA family.Curated
Contains 3 A.T hook DNA-binding domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG295015.
GeneTreeiENSGT00730000111387.
HOGENOMiHOG000076308.
HOVERGENiHBG051913.
InParanoidiP52927.
KOiK09283.
OMAiNKGPKTA.
OrthoDBiEOG7QRQXW.
PhylomeDBiP52927.
TreeFamiTF351623.

Family and domain databases

InterProiIPR020478. AT_hook-like.
IPR017956. AT_hook_DNA-bd_motif.
IPR000116. HMGA.
[Graphical view]
PfamiPF02178. AT_hook. 3 hits.
[Graphical view]
PRINTSiPR00929. ATHOOK.
PR00930. HIGHMOBLTYIY.
SMARTiSM00384. AT_hook. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARGEGAGQ PSTSAQGQPA APVPQKRGRG RPRKQQQEPT CEPSPKRPRG
60 70 80 90 100
RPKGSKNKSP SKAAQKKAET IGEKRPRGRP RKWPQQVVQK KPAQETEETS

SQESAEED
Length:108
Mass (Da):11,819
Last modified:October 1, 1996 - v1
Checksum:i40A3E38F62268C09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41621
, L41617, L41618, L41619, L41620 Genomic DNA. Translation: AAC69244.1.
X58380 mRNA. Translation: CAA41270.1.
X99915
, X99916, X99917, X99918, X99919 Genomic DNA. Translation: CAA68187.1.
AK028346 mRNA. Translation: BAC25896.1.
AK142059 mRNA. Translation: BAE24928.1.
BC052158 mRNA. Translation: AAH52158.1.
BC085085 mRNA. Translation: AAH85085.1.
CCDSiCCDS36073.1.
PIRiJC4575.
RefSeqiNP_034571.1. NM_010441.2.
UniGeneiMm.157190.

Genome annotation databases

EnsembliENSMUST00000072777; ENSMUSP00000072556; ENSMUSG00000056758.
GeneIDi15364.
KEGGimmu:15364.
UCSCiuc007hfb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41621
, L41617, L41618, L41619, L41620 Genomic DNA. Translation: AAC69244.1.
X58380 mRNA. Translation: CAA41270.1.
X99915
, X99916, X99917, X99918, X99919 Genomic DNA. Translation: CAA68187.1.
AK028346 mRNA. Translation: BAC25896.1.
AK142059 mRNA. Translation: BAE24928.1.
BC052158 mRNA. Translation: AAH52158.1.
BC085085 mRNA. Translation: AAH85085.1.
CCDSiCCDS36073.1.
PIRiJC4575.
RefSeqiNP_034571.1. NM_010441.2.
UniGeneiMm.157190.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200341. 7 interactions.
IntActiP52927. 476 interactions.
MINTiMINT-1847926.
STRINGi10090.ENSMUSP00000072556.

PTM databases

PhosphoSiteiP52927.

Proteomic databases

MaxQBiP52927.
PaxDbiP52927.
PRIDEiP52927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072777; ENSMUSP00000072556; ENSMUSG00000056758.
GeneIDi15364.
KEGGimmu:15364.
UCSCiuc007hfb.1. mouse.

Organism-specific databases

CTDi8091.
MGIiMGI:101761. Hmga2.

Phylogenomic databases

eggNOGiNOG295015.
GeneTreeiENSGT00730000111387.
HOGENOMiHOG000076308.
HOVERGENiHBG051913.
InParanoidiP52927.
KOiK09283.
OMAiNKGPKTA.
OrthoDBiEOG7QRQXW.
PhylomeDBiP52927.
TreeFamiTF351623.

Enzyme and pathway databases

ReactomeiREACT_341685. Formation of Senescence-Associated Heterochromatin Foci (SAHF).

Miscellaneous databases

ChiTaRSiHmga2. mouse.
NextBioi287988.
PROiP52927.
SOURCEiSearch...

Gene expression databases

BgeeiP52927.
CleanExiMM_HMGA2.
ExpressionAtlasiP52927. baseline and differential.
GenevisibleiP52927. MM.

Family and domain databases

InterProiIPR020478. AT_hook-like.
IPR017956. AT_hook_DNA-bd_motif.
IPR000116. HMGA.
[Graphical view]
PfamiPF02178. AT_hook. 3 hits.
[Graphical view]
PRINTSiPR00929. ATHOOK.
PR00930. HIGHMOBLTYIY.
SMARTiSM00384. AT_hook. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of the HMGI-C phosphoprotein, a nuclear protein associated with neoplastic and undifferentiated phenotypes."
    Manfioletti G., Giancotti V., Bandiera A., Buratti E., Sautiere P., Cary P., Crane-Robinson C., Coles B., Goodwin G.H.
    Nucleic Acids Res. 19:6793-6797(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of the gene coding for murine high-mobility-group protein HMGI-C."
    Manfioletti G., Rustighi A., Mantovani F., Goodwin G.H., Giancotti V.
    Gene 167:249-253(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Genomic structure and expression of the murine Hmgi-c gene."
    Zhou X., Benson K.F., Przybysz K., Liu J., Hou Y., Cherath L., Chada K.
    Nucleic Acids Res. 24:4071-4077(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo and Limb.
  6. "Phosphorylation of high-mobility group protein A2 by Nek2 kinase during the first meiotic division in mouse spermatocytes."
    Di Agostino S., Fedele M., Chieffi P., Fusco A., Rossi P., Geremia R., Sette C.
    Mol. Biol. Cell 15:1224-1232(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH NEK2, TISSUE SPECIFICITY.
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiHMGA2_MOUSE
AccessioniPrimary (citable) accession number: P52927
Secondary accession number(s): Q3UQW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.