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P52927

- HMGA2_MOUSE

UniProt

P52927 - HMGA2_MOUSE

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Protein

High mobility group protein HMGI-C

Gene

Hmga2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi24 – 3411A.T hook 1Add
BLAST
DNA bindingi44 – 5411A.T hook 2Add
BLAST
DNA bindingi71 – 8212A.T hook 3Add
BLAST

GO - Molecular functioni

  1. 5'-deoxyribose-5-phosphate lyase activity Source: UniProtKB
  2. AT DNA binding Source: UniProtKB
  3. C2H2 zinc finger domain binding Source: UniProtKB
  4. cAMP response element binding Source: UniProtKB
  5. core promoter binding Source: UniProtKB
  6. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB
  7. DNA binding, bending Source: UniProtKB
  8. DNA-dependent protein kinase activity Source: UniProtKB
  9. MH1 domain binding Source: UniProtKB
  10. MH2 domain binding Source: UniProtKB
  11. nucleosomal DNA binding Source: UniProtKB
  12. protein binding transcription factor activity Source: Ensembl
  13. regulatory region DNA binding Source: UniProtKB
  14. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: UniProtKB
  15. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI

GO - Biological processi

  1. adipose tissue development Source: MGI
  2. adrenal gland development Source: MGI
  3. base-excision repair Source: UniProtKB
  4. cell proliferation Source: MGI
  5. cell proliferation in forebrain Source: MGI
  6. chondrocyte differentiation Source: UniProtKB
  7. chondrocyte proliferation Source: UniProtKB
  8. chromosome breakage Source: UniProtKB
  9. chromosome condensation Source: UniProtKB-KW
  10. DNA damage response, detection of DNA damage Source: UniProtKB
  11. endodermal cell differentiation Source: UniProtKB
  12. epithelial to mesenchymal transition Source: UniProtKB
  13. fat cell differentiation Source: UniProtKB
  14. fat pad development Source: MGI
  15. heterochromatin assembly Source: UniProtKB
  16. histone H2A-S139 phosphorylation Source: UniProtKB
  17. male gonad development Source: MGI
  18. meiotic nuclear division Source: UniProtKB-KW
  19. mesenchymal cell differentiation Source: UniProtKB
  20. mesodermal cell differentiation Source: UniProtKB
  21. mesodermal-endodermal cell signaling Source: UniProtKB
  22. mitotic G2 DNA damage checkpoint Source: UniProtKB
  23. mitotic nuclear division Source: UniProtKB-KW
  24. negative regulation by host of viral transcription Source: UniProtKB
  25. negative regulation of apoptotic process Source: UniProtKB
  26. negative regulation of astrocyte differentiation Source: MGI
  27. negative regulation of DNA binding Source: UniProtKB
  28. negative regulation of double-strand break repair via nonhomologous end joining Source: UniProtKB
  29. negative regulation of intracellular steroid hormone receptor signaling pathway Source: Ensembl
  30. negative regulation of JAK-STAT cascade Source: Ensembl
  31. negative regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: UniProtKB
  32. negative regulation of transcription, DNA-templated Source: UniProtKB
  33. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  34. oncogene-induced cell senescence Source: UniProtKB
  35. pituitary gland development Source: MGI
  36. positive regulation of apoptotic process Source: UniProtKB
  37. positive regulation of cell cycle arrest Source: UniProtKB
  38. positive regulation of cell proliferation Source: MGI
  39. positive regulation of cellular response to X-ray Source: UniProtKB
  40. positive regulation of cellular senescence Source: UniProtKB
  41. positive regulation of gene expression Source: UniProtKB
  42. positive regulation of multicellular organism growth Source: MGI
  43. positive regulation of response to DNA damage stimulus Source: UniProtKB
  44. positive regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
  45. positive regulation of stem cell proliferation Source: UniProtKB
  46. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  47. positive regulation of transcription regulatory region DNA binding Source: UniProtKB
  48. regulation of cell cycle process Source: UniProtKB
  49. regulation of cellular response to drug Source: UniProtKB
  50. regulation of growth hormone secretion Source: MGI
  51. regulation of peptide hormone secretion Source: MGI
  52. regulation of stem cell maintenance Source: UniProtKB
  53. regulation of transcription, DNA-templated Source: UniProtKB
  54. response to virus Source: Ensembl
  55. senescence-associated heterochromatin focus assembly Source: UniProtKB
  56. signal transduction Source: Ensembl
  57. somatic stem cell maintenance Source: MGI
  58. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA condensation, Growth regulation, Meiosis, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_209428. Formation of Senescence-Associated Heterochromatin Foci (SAHF).

Names & Taxonomyi

Protein namesi
Recommended name:
High mobility group protein HMGI-C
Alternative name(s):
High mobility group AT-hook protein 2
Gene namesi
Name:Hmga2
Synonyms:Hmgic
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:101761. Hmga2.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nuclear chromosome Source: MGI
  2. nucleus Source: UniProtKB
  3. protein-DNA complex Source: UniProtKB
  4. senescence-associated heterochromatin focus Source: UniProtKB
  5. SMAD protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 108107High mobility group protein HMGI-CPRO_0000206712Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei40 – 401PhosphothreonineBy similarity
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei104 – 1041Phosphoserine1 Publication

Post-translational modificationi

Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP52927.
PaxDbiP52927.
PRIDEiP52927.

PTM databases

PhosphoSiteiP52927.

Expressioni

Tissue specificityi

Expressed in mitotic spermatogonia, meiotic spermatocytes, and postmeiotic round spermatids (at protein level).1 Publication

Developmental stagei

Expressed predominantly during embryogenesis.

Gene expression databases

BgeeiP52927.
CleanExiMM_HMGA2.
ExpressionAtlasiP52927. baseline and differential.
GenevestigatoriP52927.

Interactioni

Subunit structurei

Interacts with E4F1 (By similarity). Interacts with NEK2.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RB1P064005EBI-912574,EBI-491274From a different organism.

Protein-protein interaction databases

BioGridi200341. 7 interactions.
IntActiP52927. 5 interactions.
MINTiMINT-1847926.
STRINGi10090.ENSMUSP00000072556.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni44 – 6320Interaction with E4F1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the HMGA family.Curated
Contains 3 A.T hook DNA-binding domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG295015.
GeneTreeiENSGT00730000111387.
HOGENOMiHOG000076308.
HOVERGENiHBG051913.
InParanoidiP52927.
KOiK09283.
OMAiNSIKRSH.
OrthoDBiEOG7QRQXW.
PhylomeDBiP52927.
TreeFamiTF351623.

Family and domain databases

InterProiIPR020478. AT_hook-like.
IPR017956. AT_hook_DNA-bd_motif.
IPR000116. HMGI/HMGY.
[Graphical view]
PfamiPF02178. AT_hook. 3 hits.
[Graphical view]
PRINTSiPR00929. ATHOOK.
PR00930. HIGHMOBLTYIY.
SMARTiSM00384. AT_hook. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52927-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSARGEGAGQ PSTSAQGQPA APVPQKRGRG RPRKQQQEPT CEPSPKRPRG
60 70 80 90 100
RPKGSKNKSP SKAAQKKAET IGEKRPRGRP RKWPQQVVQK KPAQETEETS

SQESAEED
Length:108
Mass (Da):11,819
Last modified:October 1, 1996 - v1
Checksum:i40A3E38F62268C09
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L41621
, L41617, L41618, L41619, L41620 Genomic DNA. Translation: AAC69244.1.
X58380 mRNA. Translation: CAA41270.1.
X99915
, X99916, X99917, X99918, X99919 Genomic DNA. Translation: CAA68187.1.
AK028346 mRNA. Translation: BAC25896.1.
AK142059 mRNA. Translation: BAE24928.1.
BC052158 mRNA. Translation: AAH52158.1.
BC085085 mRNA. Translation: AAH85085.1.
CCDSiCCDS36073.1.
PIRiJC4575.
RefSeqiNP_034571.1. NM_010441.2.
UniGeneiMm.157190.

Genome annotation databases

EnsembliENSMUST00000072777; ENSMUSP00000072556; ENSMUSG00000056758.
GeneIDi15364.
KEGGimmu:15364.
UCSCiuc007hfb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L41621
, L41617 , L41618 , L41619 , L41620 Genomic DNA. Translation: AAC69244.1 .
X58380 mRNA. Translation: CAA41270.1 .
X99915
, X99916 , X99917 , X99918 , X99919 Genomic DNA. Translation: CAA68187.1 .
AK028346 mRNA. Translation: BAC25896.1 .
AK142059 mRNA. Translation: BAE24928.1 .
BC052158 mRNA. Translation: AAH52158.1 .
BC085085 mRNA. Translation: AAH85085.1 .
CCDSi CCDS36073.1.
PIRi JC4575.
RefSeqi NP_034571.1. NM_010441.2.
UniGenei Mm.157190.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200341. 7 interactions.
IntActi P52927. 5 interactions.
MINTi MINT-1847926.
STRINGi 10090.ENSMUSP00000072556.

PTM databases

PhosphoSitei P52927.

Proteomic databases

MaxQBi P52927.
PaxDbi P52927.
PRIDEi P52927.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000072777 ; ENSMUSP00000072556 ; ENSMUSG00000056758 .
GeneIDi 15364.
KEGGi mmu:15364.
UCSCi uc007hfb.1. mouse.

Organism-specific databases

CTDi 8091.
MGIi MGI:101761. Hmga2.

Phylogenomic databases

eggNOGi NOG295015.
GeneTreei ENSGT00730000111387.
HOGENOMi HOG000076308.
HOVERGENi HBG051913.
InParanoidi P52927.
KOi K09283.
OMAi NSIKRSH.
OrthoDBi EOG7QRQXW.
PhylomeDBi P52927.
TreeFami TF351623.

Enzyme and pathway databases

Reactomei REACT_209428. Formation of Senescence-Associated Heterochromatin Foci (SAHF).

Miscellaneous databases

ChiTaRSi HMGA2. mouse.
NextBioi 287988.
PROi P52927.
SOURCEi Search...

Gene expression databases

Bgeei P52927.
CleanExi MM_HMGA2.
ExpressionAtlasi P52927. baseline and differential.
Genevestigatori P52927.

Family and domain databases

InterProi IPR020478. AT_hook-like.
IPR017956. AT_hook_DNA-bd_motif.
IPR000116. HMGI/HMGY.
[Graphical view ]
Pfami PF02178. AT_hook. 3 hits.
[Graphical view ]
PRINTSi PR00929. ATHOOK.
PR00930. HIGHMOBLTYIY.
SMARTi SM00384. AT_hook. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of the HMGI-C phosphoprotein, a nuclear protein associated with neoplastic and undifferentiated phenotypes."
    Manfioletti G., Giancotti V., Bandiera A., Buratti E., Sautiere P., Cary P., Crane-Robinson C., Coles B., Goodwin G.H.
    Nucleic Acids Res. 19:6793-6797(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of the gene coding for murine high-mobility-group protein HMGI-C."
    Manfioletti G., Rustighi A., Mantovani F., Goodwin G.H., Giancotti V.
    Gene 167:249-253(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Genomic structure and expression of the murine Hmgi-c gene."
    Zhou X., Benson K.F., Przybysz K., Liu J., Hou Y., Cherath L., Chada K.
    Nucleic Acids Res. 24:4071-4077(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo and Limb.
  6. "Phosphorylation of high-mobility group protein A2 by Nek2 kinase during the first meiotic division in mouse spermatocytes."
    Di Agostino S., Fedele M., Chieffi P., Fusco A., Rossi P., Geremia R., Sette C.
    Mol. Biol. Cell 15:1224-1232(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH NEK2, TISSUE SPECIFICITY.
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiHMGA2_MOUSE
AccessioniPrimary (citable) accession number: P52927
Secondary accession number(s): Q3UQW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3