P52925 (HMGB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High mobility group protein B2

Alternative name(s):
High mobility group protein 2
Short name=HMG-2

Gene names

Name:	Hmgb2
Synonyms:	Hmg2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) By similarity.
Subunit structure	Component of the SET complex, which also contains SET, ANP32A, APEX1 and NME1. Directly interacts with SET. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2 By similarity.
Subcellular location	Nucleus By similarity. Chromosome By similarity.
Sequence similarities	Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains.

Ontologies

Keywords
Cellular component	Chromosome Nucleus
Domain	Repeat
Ligand	DNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	base-excision repair, DNA ligation Inferred from sequence or structural similarity. Source: UniProtKB cell cycle Inferred from expression pattern PubMed 7763232. Source: RGD nervous system development Inferred from direct assay PubMed 11279268. Source: RGD organ regeneration Inferred from expression pattern PubMed 457681. Source: RGD positive regulation of DNA metabolic process Inferred from mutant phenotype PubMed 8432381. Source: RGD positive regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 1525238. Source: RGD regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	condensed chromosome Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	DNA binding, bending Inferred from sequence or structural similarity. Source: UniProtKB double-stranded DNA binding Traceable author statement PubMed 11279268. Source: RGD lipid binding Inferred from direct assay PubMed 11279268. Source: RGD nucleosomal histone binding Inferred from direct assay PubMed 2719962. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 210	209	High mobility group protein B2		PRO_0000048537
Regions
	DNA binding	9 – 79	71	HMG box 1
	DNA binding	95 – 163	69	HMG box 2
	Compositional bias	186 – 210	25	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	30	1	N6-acetyllysine By similarity
	Modified residue	35	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52925 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 3B5CBAF61415C6F3	FASTA	210	24,159
	10 20 30 40 50 60 MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF 70 80 90 100 110 120 EDLAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS AFFLFCSEHR PKIKSEHPGL 130 140 150 160 170 180 SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK LKEKYEKDIA AYRAKGKSEV GKKGPGRPTG 190 200 210 SKKKNEPEDE EEEEEEEDDE DEEEEDEDEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat mRNA for chromosomal protein HMG2, complete cds." Yoshida M. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen, Testis and Thymus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D84418 mRNA. Translation: BAA12350.1. BC078866 mRNA. Translation: AAH78866.1. BC089854 mRNA. Translation: AAH89854.1. BC107455 mRNA. Translation: AAI07456.1.
IPI	IPI00876582.
RefSeq	NP_058883.1. NM_017187.2. XP_001063065.2. XM_001063065.3. XP_573272.3. XM_573272.4.
UniGene	Rn.218982. Rn.2874.
3D structure databases
ProteinModelPortal	P52925.
SMR	P52925. Positions 5-166.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000017635.
Proteomic databases
PaxDb	P52925.
Protocols and materials databases
DNASU	29395.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000017635; ENSRNOP00000017635; ENSRNOG00000013167. ENSRNOT00000046874; ENSRNOP00000039358; ENSRNOG00000033321.
GeneID	29395. 498072.
KEGG	rno:29395. rno:498072.
UCSC	RGD:69291. rat.
Organism-specific databases
CTD	3148. 498072.
RGD	69291. Hmgb2.
Phylogenomic databases
eggNOG	COG5648.
GeneTree	ENSGT00560000076717.
HOGENOM	HOG000197861.
HOVERGEN	HBG009000.
InParanoid	P52925.
KO	K11295.
OMA	KIKNDHP.
OrthoDB	EOG4TTGK2.
Gene expression databases
Genevestigator	P52925.
GermOnline	ENSRNOG00000013167. Rattus norvegicus. ENSRNOG00000030313. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.30.10. 2 hits.
InterPro	IPR009071. HMG_box_dom. IPR017967. HMG_boxA_CS. [Graphical view]
Pfam	PF09011. DUF1898. 1 hit. PF00505. HMG_box. 1 hit. [Graphical view]
SMART	SM00398. HMG. 2 hits. [Graphical view]
SUPFAM	SSF47095. HMG-box. 2 hits.
PROSITE	PS00353. HMG_BOX_1. 1 hit. PS50118. HMG_BOX_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	609027.

Entry information

Entry name

HMGB2_RAT

Accession

Primary (citable) accession number: P52925
Secondary accession number(s): Q5FVP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program