Reviewed, UniProtKB/Swiss-Prot P52919 (NBP1_YEAST)

Names and origin

Protein names

Recommended name:
    NAP1-binding protein

Gene names

Name:	NBP1
Ordered Locus Names:	YLR457C
ORF Names:	L9122.6

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Interacts with NDC1 and MPS2. Ref.4
Miscellaneous	Present with 339 molecules/cell in log phase SD medium. Ref.3

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	spindle pole body duplication in nuclear envelope Ref.4 Inferred from genetic interaction. Source: SGD
Cellular component	central plaque of spindle pole body Ref.4 Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P53159	1	EBI-11886,EBI-23834
BBP1	Q12365	1	EBI-11886,EBI-3448

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	NAP1-binding protein		PRO_0000096745
Amino acid modifications
	Modified residue	234	1	Phosphoserine Ref.5
	Modified residue	251	1	Phosphoserine Ref.6
	Modified residue	260	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P52919-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CFC1BE0F7295A495	FASTA	319	37,354
	10 20 30 40 50 60 MLKSVQGLWK DFFGIRDDGR KREYGSLDEV RKRSALRSRR KQMRPTGKSV LKRPRKVTDR 70 80 90 100 110 120 KTEEKIRTNR RKTPKRRLTK IFQTIRDVFS NDNENMSKMQ NVCGDMTRIL KKRSQGRPSY 130 140 150 160 170 180 MDTDTAKSRI LRSDAFKRKI SELKYNKQRI SELRSGSSDG SSGKDRNQSL YLDREILLQR 190 200 210 220 230 240 QIKKRDEKIK ALESKLQSLQ EALNYSNEKY RILEDLLDSS NIHPSYTKSR RTMSNLAREN 250 260 270 280 290 300 DEIKPLKIDL SPSPIRRTNS LFTSSPMKTY NRDGNIPEMQ PLQENISPAC PTPPYRSRET 310 EKEDETLSPI SVDFSSYLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Okuda A., Fujii-Nakata T., Kikuchi A. Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SFY526.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p." Araki Y., Lau C.K., Maekawa H., Jaspersen S.L., Giddings T.H. Jr., Schiebel E., Winey M. Mol. Biol. Cell 17:1959-1970(2006) [PubMed: 16436507] [Abstract] Cited for: INTERACTION WITH NDC1 AND MPS2.
[5]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251 AND SER-260, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D43632 Genomic DNA. Translation: BAA07740.1. U22383 Genomic DNA. Translation: AAB64721.1.
PIR	S59416.
RefSeq	NP_013562.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3852N.
IntAct	P52919. 8 interactions.
STRING	P52919.
Genome annotation databases
Ensembl	YLR457C; YLR457C; YLR457C; Saccharomyces cerevisiae. [Genome view]
GeneID	851180.
KEGG	sce:YLR457C.
NMPDR	fig|4932.3.peg.4593.
Organism-specific databases
CYGD	YLR457c.
SGD	S000004449. NBP1.
Phylogenomic databases
HOGENOM	P52919.
OMA	FTSSPIR
OrthoDB	EOG9QNPCN
Gene expression databases
ArrayExpress	P52919.
Genevestigator	P52919.
GermOnline	YLR457C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013743. NBP1_fun. [Graphical view]
Pfam	PF08537. NBP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968004.

Entry information

Entry name

NBP1_YEAST

Accession

Primary (citable) accession number: P52919

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)