P52918 (MSN5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein MSN5

Gene names

Name:	MSN5
Ordered Locus Names:	YDR335W
ORF Names:	D9651.5

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with CEX1. Ref.5
Miscellaneous	Present with 3500 molecules/cell in log phase SD medium.

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA re-export from nucleus Inferred from genetic interaction PubMed 20032305. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 17020589. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD nucleus Inferred from direct assay PubMed 10485850PubMed 10545454PubMed 17020589. Source: SGD
Molecular_function	importin-alpha export receptor activity Inferred from direct assay PubMed 9214382. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1224	1224	Protein MSN5		PRO_0000096599
Experimental info
	Sequence conflict	886	1	D → G in CAA63705. Ref.1
	Sequence conflict	1011	1	S → R in CAA63705. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52918 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C47A6767CBA44486	FASTA	1,224	142,118
	10 20 30 40 50 60 MDSTGASQIV SALDVIYSPK SNNSQRQEAQ KFLDEVKLCS ESPFWGYEIA LQNPTNSILK 70 80 90 100 110 120 YFGLGLLDHA VKKNWNDYDE GKRVALRKWV MELNFGVQDY DTRYIKEKLA TLWVEVAKRT 130 140 150 160 170 180 WGEALKQTNP TEEQLLTSWV DMDNNLFELW NINQSSRELA LIIFRILFED VFLLDDLIVL 190 200 210 220 230 240 KRMTVIQPLC VMIVCPIEVF AIKYKFSDKW TKFKANEEGW FSVWIPELNN ALQQNNSEYI 250 260 270 280 290 300 IRLLETLKTC LNWPLTEVIV RNDVLSSLLT CLSSNIPRAQ SMALDSIHIL LTRPYSNESH 310 320 330 340 350 360 YQMTIDRVFD NMDLLDSVYE SLLFDPTDDI DETKYPIIKK FVDMISCLYV CVPKIKETNG 370 380 390 400 410 420 QIQKYFKLVL KTTYNPSLIV SGLTLDLWCT CLRNDEYLPK LEKYVIPDLL QFAADALVYY 430 440 450 460 470 480 EQIDGHISKK FAEIDFQSKS EFQTFCSTYR KRIRDIIRLI SCVELDLTYD WLNNRLNNYF 490 500 510 520 530 540 SSPFGQQVLS STFLDHKLEP YLGALSQYMI VECFINGCIR WKIWYPTGDD YDEKLDSILQ 550 560 570 580 590 600 KLEILSNQLI ALNLREPLLL KKQIQNFALF LTMLKDNVLF TLLEKIITSA TMDYPEINLE 610 620 630 640 650 660 ERGAESDAVR DLRYACGIEL NRMALLMPES LKKIYPDLES VIARIMPNLS YHEKISFKSF 670 680 690 700 710 720 LLIIVLKSSL DMKEERFAAI VDPELLAWSD KTTVVGLSDL HWFMERLGIV QIAEYFQRRD 730 740 750 760 770 780 IDENSDLLSI PIDDEGKELK SELTKRWQSL FPVRATRMFI HYSMQSIKTD EEFKMLQDLW 790 800 810 820 830 840 RPRIVPILPY ITRLLYQLQS YHDPDNWKGL PTVVQSFVKY STIERFWEAG ASNKSKDEFI 850 860 870 880 890 900 DEHMKAMQTL RDFADSVGHI IRYTREYTLL VLSAISSLGS VFYLLDESPD LLLNSIAIFK 910 920 930 940 950 960 PGSNEISPGV STHGWKHIMN IAIRPILKGC PKDCLGKFMP AFLPKLFEIL DLLLCQKWSS 970 980 990 1000 1010 1020 HMNDMDMNPV PTDDDQMTEE ILEENLLRQL TTVVVRIVID CVGQGNANPN SAKSRLNNHQ 1030 1040 1050 1060 1070 1080 MEMRKIIFND LNTLAPFLKL LNHLISFKDT KCSFNSILVM KCCLTSVLNQ NNTVDEYFTF 1090 1100 1110 1120 1130 1140 EVMKNLLLNV LCNSAFKDSF HEALYAFTVI FLTLCKEYPS ARAFLFEISN GYNIDELYRN 1150 1160 1170 1180 1190 1200 LRSVDEYKTQ RALMIDFIDW VKSTSGKEDG NVDHAGDERK RQEKREAILK KANERLIKKN 1210 1220 KENGDMLDDP NIEDGAVGNL FDDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Alepuz P.M., Estruch F. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Cex1p is a novel cytoplasmic component of the Saccharomyces cerevisiae nuclear tRNA export machinery." McGuire A.T., Mangroo D. EMBO J. 26:288-300(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CEX1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X93302 mRNA. Translation: CAA63705.1. U51032 Genomic DNA. Translation: AAB64771.1. BK006938 Genomic DNA. Translation: DAA12177.1.
PIR	S70100.
RefSeq	NP_010622.1. NM_001180643.1.
3D structure databases
ProteinModelPortal	P52918.
SMR	P52918. Positions 1-711.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1459N.
IntAct	P52918. 24 interactions.
MINT	MINT-388409.
STRING	4932.YDR335W.
Protein family/group databases
TCDB	9.A.50.1.1. nuclear t-RNA exporter (t-Exporter) family.
Proteomic databases
PaxDb	P52918.
PeptideAtlas	P52918.
PRIDE	P52918.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR335W; YDR335W; YDR335W.
GeneID	851935.
KEGG	sce:YDR335W.
Organism-specific databases
CYGD	YDR335w.
SGD	S000002743. MSN5.
Phylogenomic databases
eggNOG	NOG296759.
GeneTree	ENSGT00390000013979.
HOGENOM	HOG000248154.
KO	K14289.
OMA	FADSVGH.
OrthoDB	EOG46T68M.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29891-MONOMER.
Gene expression databases
Genevestigator	P52918.
GermOnline	YDR335W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	970004.

Entry information

Entry name

MSN5_YEAST

Accession

Primary (citable) accession number: P52918
Secondary accession number(s): D6VSW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program