Reviewed, UniProtKB/Swiss-Prot P52902 (ODPA_PEA)

Names and origin

Protein names	Recommended name:     Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial       Short name=PDHE1-A     EC=1.2.4.1
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Catalytic activity	Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate.
Enzyme regulation	E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit By similarity.
Subunit structure	Tetramer of 2 alpha and 2 beta subunits By similarity.
Subcellular location	Mitochondrion matrix.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyruvate Thiamine pyrophosphate
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	pyruvate dehydrogenase (acetyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 397		Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial		PRO_0000020454

Sequences

	Sequence		Length	Mass (Da)
	P52902-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3C0257CB0032E50B	FASTA	397	43,530
	10 20 30 40 50 60 MALSRLSSSS SSSNGSNLFN PFSAAFTLNR PISSDTTATL TIETSLPFTA HNCDPPSRSV 70 80 90 100 110 120 TTSPSELLSF FRTMALMRRM EIAADSLYKA NLIRGFCHLY DGQEAVAVGM EAGTTKKDCI 130 140 150 160 170 180 ITAYRDHCTF LGRGGTLLRV YAELMGRRDG CSKGKGGSMH FYKKDSGFYG GHGIVGAQVP 190 200 210 220 230 240 LGCGLAFGQK YLKDESVTFA LYGDGAANQG QLFEALNISA LWDLPAILVC ENNHYGMGTA 250 260 270 280 290 300 TWRSAKSPAY FKRGDYVPGL KVDGMDALAV KQACKFAKEH ALKNGPIILE MDTYRYHGHS 310 320 330 340 350 360 MSDPGSTYRT RDEISGVRQE RDPIERVRKL LLSHDIATEK ELKDTEKEVR KEVDEAIAKA 370 380 390 KDSPMPDPSD LFSNVYVKGY GVEAFGVDRK EVRVTLP

References

[1]	Luethy M.H., Miernyk J.A., Randall D.D. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U51918 mRNA. Translation: AAA97411.1.
PIR	T06531.
3D structure databases
HSSP	HSSP built from PDB template 1NI4 based on UniProtKB P08559.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52902. 1 interaction.
Enzyme and pathway databases
BRENDA	1.2.4.1. 287.
Family and domain databases
InterPro	IPR001017. DH_E1. IPR017597. Pyrv_DH_E1_asu_subgrp-y. [Graphical view]
Pfam	PF00676. E1_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03182. PDH_E1_alph_y. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODPA_PEA

Accession

Primary (citable) accession number: P52902

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)