Reviewed,
UniProtKB/Swiss-Prot P52901 (ODPA1_ARATH)
Last modified
June 16, 2009.
Version 80.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial Short name=PDHE1-A EC=1.2.4.1 | ||||
| Gene names |
| ||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||
| Taxonomic identifier | 3702 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 389 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). |
| Catalytic activity | Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2. |
| Cofactor | Thiamine pyrophosphate. |
| Enzyme regulation | E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit By similarity. |
| Subunit structure | Tetramer of 2 alpha and 2 beta subunits By similarity. |
| Subcellular location |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | Pyruvate Thiamine pyrophosphate |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW response to cadmium ionInferred from expression pattern. Source: TAIR |
| Cellular component | cytosol Inferred from direct assay. Source: TAIR mitochondrial matrixInferred from electronic annotation. Source: UniProtKB-SubCell nucleusInferred from direct assay. Source: TAIR |
| Molecular function | pyruvate dehydrogenase (acetyl-transferring) activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 32 | 32 | Mitochondrion Potential | ||||||
| Chain | 33 – 389 | 357 | Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial | PRO_0000020453 | |||||
Experimental info | |||||||||
| Sequence conflict | 83 – 84 | 2 | KL → NV in AAA86507. Ref.1 | ||||||
| Sequence conflict | 362 | 1 | E → D in AAK26016. Ref.3 | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cDNA encoding the Arabidopsis thaliana E1 alpha-subunit." Luethy M.H., Miernyk J.A., Randall D.D. Gene 164:251-254(1995) [PubMed: 7590338] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
Cross-references
Sequence databases | |
|---|---|
| U21214 mRNA. Translation: AAA86507.1. AC007258 Genomic DNA. Translation: AAD39331.1. AF360306 mRNA. Translation: AAK26016.1. BT000974 mRNA. Translation: AAN41374.1. AY087667 mRNA. Translation: AAM65205.1. | |
| IPI | IPI00531318. |
| PIR | B96623. JC4358. |
| RefSeq | NP_176198.1. |
| UniGene | At.23186 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1NI4 based on UniProtKB P08559. |
| ModBase | Search... |
2-D gel databases | |
| SWISS-2DPAGE | P52901. |
Proteomic databases | |
| PRIDE | P52901. |
Genome annotation databases | |
| GeneID | 842284. |
| GenomeReviews | Gene locus AT1G59900 in contig CT485782_GR. |
| KEGG | ath:AT1G59900. |
| NMPDR | fig|3702.1.peg.5381. |
Organism-specific databases | |
| GeneFarm | 4372. 441. |
| TAIR | At1g59900. |
Phylogenomic databases | |
| OMA | P52901. LEYETYR. |
Enzyme and pathway databases | |
| BRENDA | 1.2.4.1. 302. |
Gene expression databases | |
| ArrayExpress | P52901. |
| GermOnline | AT1G59900. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001017. DH_E1. IPR017597. Pyrv_DH_E1_asu_subgrp-y. [Graphical view] |
| Pfam | PF00676. E1_dh. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR03182. PDH_E1_alph_y. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | ODPA1_ARATH | ||||||||
| Accession | Primary (citable) accession number: P52901 Secondary accession number(s): Q9C5E3, Q9SXC2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||

Clusters with


