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P52901

- ODPA1_ARATH

UniProt

P52901 - ODPA1_ARATH

Protein

Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial

Gene

E1 ALPHA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (10 Jan 2003)
      Previous versions | rss
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    Functioni

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

    Catalytic activityi

    Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

    Cofactori

    Thiamine pyrophosphate.

    Enzyme regulationi

    E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.By similarity

    GO - Molecular functioni

    1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-KW
    2. response to cadmium ion Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Pyruvate, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciARA:AT1G59900-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial (EC:1.2.4.1)
    Short name:
    PDHE1-A
    Gene namesi
    Name:E1 ALPHA
    Ordered Locus Names:At1g59900
    ORF Names:F23H11.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G59900.

    Subcellular locationi

    Mitochondrion matrix 1 Publication

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. mitochondrial matrix Source: UniProtKB-SubCell
    3. mitochondrion Source: TAIR
    4. nucleus Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3232MitochondrionSequence AnalysisAdd
    BLAST
    Chaini33 – 389357Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrialPRO_0000020453Add
    BLAST

    Proteomic databases

    PaxDbiP52901.
    PRIDEiP52901.

    2D gel databases

    SWISS-2DPAGEP52901.

    Expressioni

    Tissue specificityi

    Expressed in roots, rosettes and flowers.1 Publication

    Gene expression databases

    ArrayExpressiP52901.
    GenevestigatoriP52901.

    Interactioni

    Subunit structurei

    Tetramer of 2 alpha and 2 beta subunits.By similarity

    Protein-protein interaction databases

    BioGridi27509. 1 interaction.
    IntActiP52901. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP52901.
    SMRiP52901. Positions 47-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1071.
    HOGENOMiHOG000281336.
    InParanoidiP52901.
    KOiK00161.
    OMAiIANGPIV.
    PhylomeDBiP52901.

    Family and domain databases

    Gene3Di3.40.50.970. 1 hit.
    InterProiIPR001017. DH_E1.
    IPR017597. Pyrv_DH_E1_asu_subgrp-y.
    IPR029061. THDP-binding.
    [Graphical view]
    PfamiPF00676. E1_dh. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 1 hit.
    TIGRFAMsiTIGR03182. PDH_E1_alph_y. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52901-1 [UniParc]FASTAAdd to Basket

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    MALSRLSSRS NIITRPFSAA FSRLISTDTT PITIETSLPF TAHLCDPPSR    50
    SVESSSQELL DFFRTMALMR RMEIAADSLY KAKLIRGFCH LYDGQEAVAI 100
    GMEAAITKKD AIITAYRDHC IFLGRGGSLH EVFSELMGRQ AGCSKGKGGS 150
    MHFYKKESSF YGGHGIVGAQ VPLGCGIAFA QKYNKEEAVT FALYGDGAAN 200
    QGQLFEALNI SALWDLPAIL VCENNHYGMG TAEWRAAKSP SYYKRGDYVP 250
    GLKVDGMDAF AVKQACKFAK QHALEKGPII LEMDTYRYHG HSMSDPGSTY 300
    RTRDEISGVR QERDPIERIK KLVLSHDLAT EKELKDMEKE IRKEVDDAIA 350
    KAKDCPMPEP SELFTNVYVK GFGTESFGPD RKEVKASLP 389
    Length:389
    Mass (Da):43,059
    Last modified:January 10, 2003 - v2
    Checksum:i637E5BE5E86DCCAE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti83 – 842KL → NV in AAA86507. (PubMed:7590338)Curated
    Sequence conflicti362 – 3621E → D in AAK26016. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21214 mRNA. Translation: AAA86507.1.
    AC007258 Genomic DNA. Translation: AAD39331.1.
    CP002684 Genomic DNA. Translation: AEE33638.1.
    AF360306 mRNA. Translation: AAK26016.1.
    BT000974 mRNA. Translation: AAN41374.1.
    AY087667 mRNA. Translation: AAM65205.1.
    PIRiB96623.
    JC4358.
    RefSeqiNP_176198.1. NM_104682.2.
    UniGeneiAt.23186.

    Genome annotation databases

    EnsemblPlantsiAT1G59900.1; AT1G59900.1; AT1G59900.
    GeneIDi842284.
    KEGGiath:AT1G59900.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21214 mRNA. Translation: AAA86507.1 .
    AC007258 Genomic DNA. Translation: AAD39331.1 .
    CP002684 Genomic DNA. Translation: AEE33638.1 .
    AF360306 mRNA. Translation: AAK26016.1 .
    BT000974 mRNA. Translation: AAN41374.1 .
    AY087667 mRNA. Translation: AAM65205.1 .
    PIRi B96623.
    JC4358.
    RefSeqi NP_176198.1. NM_104682.2.
    UniGenei At.23186.

    3D structure databases

    ProteinModelPortali P52901.
    SMRi P52901. Positions 47-368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 27509. 1 interaction.
    IntActi P52901. 1 interaction.

    2D gel databases

    SWISS-2DPAGE P52901.

    Proteomic databases

    PaxDbi P52901.
    PRIDEi P52901.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G59900.1 ; AT1G59900.1 ; AT1G59900 .
    GeneIDi 842284.
    KEGGi ath:AT1G59900.

    Organism-specific databases

    GeneFarmi 4372. 441.
    TAIRi AT1G59900.

    Phylogenomic databases

    eggNOGi COG1071.
    HOGENOMi HOG000281336.
    InParanoidi P52901.
    KOi K00161.
    OMAi IANGPIV.
    PhylomeDBi P52901.

    Enzyme and pathway databases

    BioCyci ARA:AT1G59900-MONOMER.

    Miscellaneous databases

    PROi P52901.

    Gene expression databases

    ArrayExpressi P52901.
    Genevestigatori P52901.

    Family and domain databases

    Gene3Di 3.40.50.970. 1 hit.
    InterProi IPR001017. DH_E1.
    IPR017597. Pyrv_DH_E1_asu_subgrp-y.
    IPR029061. THDP-binding.
    [Graphical view ]
    Pfami PF00676. E1_dh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 1 hit.
    TIGRFAMsi TIGR03182. PDH_E1_alph_y. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cDNA encoding the Arabidopsis thaliana E1 alpha-subunit."
      Luethy M.H., Miernyk J.A., Randall D.D.
      Gene 164:251-254(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiODPA1_ARATH
    AccessioniPrimary (citable) accession number: P52901
    Secondary accession number(s): Q9C5E3, Q9SXC2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 10, 2003
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names

    External Data

    Dasty 3