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P52897 (PGFS2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Prostaglandin F synthase 2
Short name=PGF 2
Short name=PGF synthase 2
Short name=PGFS2
EC=1.1.1.188
Alternative name(s):
Prostaglandin F synthase II
Short name=PGFSII
Prostaglandin-D2 11 reductase 2
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the reduction of PGD2 and PGH2 to PGF(2 alpha) and a stereoisomer, respectively. It has a broad substrate specificity and reduces also other carbonyl compounds.

Catalytic activity

(5Z,13E)-(15S)-9-alpha,11-alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH.

Pathway

Lipid metabolism; prostaglandin biosynthesis.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the aldo/keto reductase family.

Ontologies

Keywords
   Biological processFatty acid biosynthesis
Lipid synthesis
Prostaglandin biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processprostaglandin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionprostaglandin-F synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Prostaglandin F synthase 2
PRO_0000124647

Regions

Nucleotide binding13 – 2210NADP Potential
Nucleotide binding217 – 28064NADP By similarity

Sites

Active site551Proton donor By similarity
Binding site1171Substrate By similarity
Site841Lowers pKa of active site Tyr By similarity

Sequences

Sequence LengthMass (Da)Tools
P52897 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 21A5BE0668D07A2C

FASTA32336,742
        10         20         30         40         50         60 
MDPKSQRVKF NDGHFIPVLG FGTYAPEEVP KSEALEATKF AIEVGFRHVD SAHLYQNEEQ 

        70         80         90        100        110        120 
VGQAIRSKIA DGTVKREDIF YTSKLWCNSL QPELVRPALE KSLQNLQLDY VDLYIIHSPV 

       130        140        150        160        170        180 
SLKPGNKFVP KDESGKLIFD SVDLCHTWEA LEKCKDAGLT KSIGVSNFNH KQLEKILNKP 

       190        200        210        220        230        240 
GLKYKPVCNQ VECHPYLNQS KLLEFCKSHD IVLVAYAALG AQLLSEWVNS NNPVLLEDPV 

       250        260        270        280        290        300 
LCAIAKKHKQ TPALVALRYQ VQRGVVVLAK SFNKKRIKEN MQVFDFELTP EDMKAIDGLN 

       310        320 
RNTRYYDFQQ GIGHPEYPFS EEY

« Hide

References

[1]"A lung type prostaglandin F synthase is expressed in bovine liver: cDNA sequence and expression in E. coli."
Kuchinke W., Barski O., Watanabe K., Hayaishi O.
Biochem. Biophys. Res. Commun. 183:1238-1246(1992) [PubMed: 1339268] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M86544 mRNA. Translation: AAA30730.1.
IPIIPI00701050.
PIRJH0575. E75572.
UniGeneBt.64820.

3D structure databases

ProteinModelPortalP52897.
SMRP52897. Positions 2-323.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000024086; ENSBTAP00000024086; ENSBTAG00000022570.

Phylogenomic databases

GeneTreeENSGT00550000074107.
HOVERGENHBG000020.
InParanoidP52897.
PhylomeDBP52897.

Family and domain databases

InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
Gene3DG3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHERPTHR11732. Aldo/ket_red. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFPIRSF000097. AKR. 1 hit.
PRINTSPR00069. ALDKETRDTASE.
SUPFAMSSF51430. Aldo/ket_red. 1 hit.
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGFS2_BOVIN
AccessionPrimary (citable) accession number: P52897
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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