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Protein

Alanine aminotransferase 2

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transfer of C3 units between the cytosol of mesophyll and bundle sheath cells to maintain a nitrogen-carbon balance in the C4-dicarboxylic pathway.

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathway: C4 acid pathway

This protein is involved in the pathway C4 acid pathway, which is part of Photosynthesis.
View all proteins of this organism that are known to be involved in the pathway C4 acid pathway and in Photosynthesis.

Pathway: L-alanine degradation via transaminase pathway

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine aminotransferase 2
This subpathway is part of the pathway L-alanine degradation via transaminase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from L-alanine, the pathway L-alanine degradation via transaminase pathway and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.6.1.2. 2687.
SABIO-RKP52894.
UniPathwayiUPA00322.
UPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine aminotransferase 2 (EC:2.6.1.2)
Short name:
ALAAT-2
Alternative name(s):
Glutamate pyruvate transaminase 2
Short name:
GPT
Glutamic--alanine transaminase 2
Glutamic--pyruvic transaminase 2
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP52894.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482Alanine aminotransferase 2PRO_0000123939Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei299 – 2991N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1
482
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Helixi12 – 165Combined sources
Helixi20 – 223Combined sources
Helixi24 – 3815Combined sources
Beta strandi42 – 476Combined sources
Helixi56 – 583Combined sources
Helixi65 – 7511Combined sources
Helixi77 – 815Combined sources
Helixi85 – 884Combined sources
Helixi91 – 10111Combined sources
Beta strandi109 – 1113Combined sources
Helixi119 – 13315Combined sources
Helixi139 – 1413Combined sources
Beta strandi142 – 1487Combined sources
Helixi149 – 15911Combined sources
Beta strandi165 – 1728Combined sources
Helixi176 – 1838Combined sources
Beta strandi187 – 1926Combined sources
Turni195 – 1984Combined sources
Helixi203 – 21513Combined sources
Beta strandi219 – 22810Combined sources
Turni230 – 2323Combined sources
Helixi238 – 25114Combined sources
Beta strandi254 – 2585Combined sources
Turni260 – 2634Combined sources
Helixi275 – 2817Combined sources
Beta strandi285 – 2873Combined sources
Beta strandi291 – 2999Combined sources
Turni300 – 3023Combined sources
Helixi305 – 3073Combined sources
Beta strandi310 – 3167Combined sources
Helixi321 – 33010Combined sources
Helixi337 – 34711Combined sources
Beta strandi352 – 3543Combined sources
Helixi356 – 38328Combined sources
Beta strandi395 – 3995Combined sources
Helixi407 – 41610Combined sources
Helixi420 – 43213Combined sources
Beta strandi437 – 4393Combined sources
Turni440 – 4423Combined sources
Beta strandi450 – 4556Combined sources
Turni459 – 4613Combined sources
Helixi462 – 48019Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TCMX-ray2.71A/B1-482[»]
ProteinModelPortaliP52894.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

P52894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATVAVDNL NPKVLKCEYA VRGEIVIHAQ RLQEQLKTQP GSLPFDEILY
60 70 80 90 100
CNIGNPQSLG QQPVTFFREV LALCDHPDLL QREEIKTLFS ADSISRAKQI
110 120 130 140 150
LAMIPGRATG AYSHSQGIKG LRDAIASGIA SRDGFPANAD DIFLTDGASP
160 170 180 190 200
GVHLMMQLLI RNEKDGILVP IPQYPLYSAS IALHGGALVP YYLNESTGWG
210 220 230 240 250
LETSDVKKQL EDARSRGINV RALVVINPGN PTGQVLAEEN QYDIVKFCKN
260 270 280 290 300
EGLVLLADEV YQENIYVDNK KFHSFKKIVR SLGYGEEDLP LVSYQSVSKG
310 320 330 340 350
YYGECGKRGG YFEITGFSAP VREQIYKIAS VNLCSNITGQ ILASLVMNPP
360 370 380 390 400
KASDESYASY KAEKDGILAS LARRAKALEH AFNKLEGITC NEAEGAMYVF
410 420 430 440 450
PQICLPQKAI EAAKAANKAP DAFYALRLLE STGIVVVPGS GFGQVPGTWH
460 470 480
FRCTILPQED KIPAVISRFT VFHEAFMSEY RD
Length:482
Mass (Da):52,877
Last modified:October 1, 1996 - v1
Checksum:iE84176F7DFB6B2FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26322 mRNA. Translation: CAA81231.1.
PIRiS42535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26322 mRNA. Translation: CAA81231.1.
PIRiS42535.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TCMX-ray2.71A/B1-482[»]
ProteinModelPortaliP52894.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiP52894.

Enzyme and pathway databases

UniPathwayiUPA00322.
UPA00528; UER00586.
BRENDAi2.6.1.2. 2687.
SABIO-RKP52894.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Hypoxically inducible barley alanine aminotransferase: cDNA cloning and expression analysis."
    Muench D.G., Good A.G.
    Plant Mol. Biol. 24:417-427(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Himalaya.
    Tissue: Root.

Entry informationi

Entry nameiALA2_HORVU
AccessioniPrimary (citable) accession number: P52894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.