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P52894

- ALA2_HORVU

UniProt

P52894 - ALA2_HORVU

Protein

Alanine aminotransferase 2

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Transfer of C3 units between the cytosol of mesophyll and bundle sheath cells to maintain a nitrogen-carbon balance in the C4-dicarboxylic pathway.

    Catalytic activityi

    L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    GO - Molecular functioni

    1. L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. L-alanine catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00322.
    UPA00528; UER00586.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine aminotransferase 2 (EC:2.6.1.2)
    Short name:
    ALAAT-2
    Alternative name(s):
    Glutamate pyruvate transaminase 2
    Short name:
    GPT
    Glutamic--alanine transaminase 2
    Glutamic--pyruvic transaminase 2
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiP52894.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482Alanine aminotransferase 2PRO_0000123939Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei299 – 2991N6-(pyridoxal phosphate)lysineBy similarity

    Expressioni

    Gene expression databases

    GenevestigatoriP52894.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    Secondary structure

    1
    482
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 93
    Helixi12 – 165
    Helixi20 – 223
    Helixi24 – 3815
    Beta strandi42 – 476
    Helixi56 – 583
    Helixi65 – 7511
    Helixi77 – 815
    Helixi85 – 884
    Helixi91 – 10111
    Beta strandi109 – 1113
    Helixi119 – 13315
    Helixi139 – 1413
    Beta strandi142 – 1487
    Helixi149 – 15911
    Beta strandi165 – 1728
    Helixi176 – 1838
    Beta strandi187 – 1926
    Turni195 – 1984
    Helixi203 – 21513
    Beta strandi219 – 22810
    Turni230 – 2323
    Helixi238 – 25114
    Beta strandi254 – 2585
    Turni260 – 2634
    Helixi275 – 2817
    Beta strandi285 – 2873
    Beta strandi291 – 2999
    Turni300 – 3023
    Helixi305 – 3073
    Beta strandi310 – 3167
    Helixi321 – 33010
    Helixi337 – 34711
    Beta strandi352 – 3543
    Helixi356 – 38328
    Beta strandi395 – 3995
    Helixi407 – 41610
    Helixi420 – 43213
    Beta strandi437 – 4393
    Turni440 – 4423
    Beta strandi450 – 4556
    Turni459 – 4613
    Helixi462 – 48019

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TCMX-ray2.71A/B1-482[»]
    ProteinModelPortaliP52894.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P52894-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAATVAVDNL NPKVLKCEYA VRGEIVIHAQ RLQEQLKTQP GSLPFDEILY    50
    CNIGNPQSLG QQPVTFFREV LALCDHPDLL QREEIKTLFS ADSISRAKQI 100
    LAMIPGRATG AYSHSQGIKG LRDAIASGIA SRDGFPANAD DIFLTDGASP 150
    GVHLMMQLLI RNEKDGILVP IPQYPLYSAS IALHGGALVP YYLNESTGWG 200
    LETSDVKKQL EDARSRGINV RALVVINPGN PTGQVLAEEN QYDIVKFCKN 250
    EGLVLLADEV YQENIYVDNK KFHSFKKIVR SLGYGEEDLP LVSYQSVSKG 300
    YYGECGKRGG YFEITGFSAP VREQIYKIAS VNLCSNITGQ ILASLVMNPP 350
    KASDESYASY KAEKDGILAS LARRAKALEH AFNKLEGITC NEAEGAMYVF 400
    PQICLPQKAI EAAKAANKAP DAFYALRLLE STGIVVVPGS GFGQVPGTWH 450
    FRCTILPQED KIPAVISRFT VFHEAFMSEY RD 482
    Length:482
    Mass (Da):52,877
    Last modified:October 1, 1996 - v1
    Checksum:iE84176F7DFB6B2FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26322 mRNA. Translation: CAA81231.1.
    PIRiS42535.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26322 mRNA. Translation: CAA81231.1 .
    PIRi S42535.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TCM X-ray 2.71 A/B 1-482 [» ]
    ProteinModelPortali P52894.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P52894.

    Enzyme and pathway databases

    UniPathwayi UPA00322 .
    UPA00528 ; UER00586 .

    Gene expression databases

    Genevestigatori P52894.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Hypoxically inducible barley alanine aminotransferase: cDNA cloning and expression analysis."
      Muench D.G., Good A.G.
      Plant Mol. Biol. 24:417-427(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Himalaya.
      Tissue: Root.

    Entry informationi

    Entry nameiALA2_HORVU
    AccessioniPrimary (citable) accession number: P52894
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3