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P52894 (ALA2_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alanine aminotransferase 2

Short name=ALAAT-2
EC=2.6.1.2
Alternative name(s):
Glutamate pyruvate transaminase 2
Short name=GPT
Glutamic--alanine transaminase 2
Glutamic--pyruvic transaminase 2
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfer of C3 units between the cytosol of mesophyll and bundle sheath cells to maintain a nitrogen-carbon balance in the C4-dicarboxylic pathway.

Catalytic activity

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactor

Pyridoxal phosphate.

Pathway

Photosynthesis; C4 acid pathway.

Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 482482Alanine aminotransferase 2
PRO_0000123939

Amino acid modifications

Modified residue2991N6-(pyridoxal phosphate)lysine By similarity

Secondary structure

.................................................................................. 482
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P52894 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: E84176F7DFB6B2FC

FASTA48252,877
        10         20         30         40         50         60 
MAATVAVDNL NPKVLKCEYA VRGEIVIHAQ RLQEQLKTQP GSLPFDEILY CNIGNPQSLG 

        70         80         90        100        110        120 
QQPVTFFREV LALCDHPDLL QREEIKTLFS ADSISRAKQI LAMIPGRATG AYSHSQGIKG 

       130        140        150        160        170        180 
LRDAIASGIA SRDGFPANAD DIFLTDGASP GVHLMMQLLI RNEKDGILVP IPQYPLYSAS 

       190        200        210        220        230        240 
IALHGGALVP YYLNESTGWG LETSDVKKQL EDARSRGINV RALVVINPGN PTGQVLAEEN 

       250        260        270        280        290        300 
QYDIVKFCKN EGLVLLADEV YQENIYVDNK KFHSFKKIVR SLGYGEEDLP LVSYQSVSKG 

       310        320        330        340        350        360 
YYGECGKRGG YFEITGFSAP VREQIYKIAS VNLCSNITGQ ILASLVMNPP KASDESYASY 

       370        380        390        400        410        420 
KAEKDGILAS LARRAKALEH AFNKLEGITC NEAEGAMYVF PQICLPQKAI EAAKAANKAP 

       430        440        450        460        470        480 
DAFYALRLLE STGIVVVPGS GFGQVPGTWH FRCTILPQED KIPAVISRFT VFHEAFMSEY 


RD 

« Hide

References

[1]"Hypoxically inducible barley alanine aminotransferase: cDNA cloning and expression analysis."
Muench D.G., Good A.G.
Plant Mol. Biol. 24:417-427(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Himalaya.
Tissue: Root.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z26322 mRNA. Translation: CAA81231.1.
PIRS42535.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3TCMX-ray2.71A/B1-482[»]
ProteinModelPortalP52894.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP52894.

Enzyme and pathway databases

UniPathwayUPA00322.
UPA00528; UER00586.

Gene expression databases

GenevestigatorP52894.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Entry information

Entry nameALA2_HORVU
AccessionPrimary (citable) accession number: P52894
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 16, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways