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P52893

- ALAM_YEAST

UniProt

P52893 - ALAM_YEAST

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Protein

Probable alanine aminotransferase, mitochondrial

Gene
ALT1, YLR089C, L9449.15
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pyridoxal phosphate.

Pathwayi

GO - Molecular functioni

  1. L-alanine:2-oxoglutarate aminotransferase activity Source: SGD
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. alanine biosynthetic process Source: SGD
  2. alanine catabolic process Source: SGD
  3. chronological cell aging Source: SGD
  4. L-alanine catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YLR089C-MONOMER.
UniPathwayiUPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alanine aminotransferase, mitochondrial (EC:2.6.1.2)
Alternative name(s):
Glutamate pyruvate transaminase
Short name:
GPT
Glutamic--alanine transaminase
Glutamic--pyruvic transaminase
Gene namesi
Name:ALT1
Ordered Locus Names:YLR089C
ORF Names:L9449.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR089c.
SGDiS000004079. ALT1.

Subcellular locationi

Mitochondrion matrix 1 Publication

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 592Probable alanine aminotransferase, mitochondrialPRO_0000001220
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771Phosphoserine1 Publication
Modified residuei412 – 4121N6-(pyridoxal phosphate)lysine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP52893.
PaxDbiP52893.
PeptideAtlasiP52893.

Expressioni

Gene expression databases

GenevestigatoriP52893.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

BioGridi31362. 154 interactions.
IntActiP52893. 5 interactions.
MINTiMINT-2492558.
STRINGi4932.YLR089C.

Structurei

3D structure databases

ProteinModelPortaliP52893.
SMRiP52893. Positions 111-591.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0436.
GeneTreeiENSGT00650000093331.
HOGENOMiHOG000215020.
KOiK00814.
OMAiFPANPQD.
OrthoDBiEOG7GXPMR.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52893-1 [UniParc]FASTAAdd to Basket

« Hide

MLSLSAKNHF TVSNSITHVI KSYHIRTLTS SAEKMPHITT PFSTSASSTK    50
LKAFRKVRPV LQRHSSSWIV AQNHRRSLSG QSSLNDLRHL NRFPHHTLKT 100
SNNEFYPAEQ LTLEDVNENV LKAKYAVRGA IPMRAEELKA QLEKDPQSLP 150
FDRIINANIG NPQQLQQKPL TYYRQVLSLL QYPELLNQNE QQLVDSKLFK 200
LDAIKRAKSL MEDIGGSVGA YSSSQGVEGI RKSVAEFITK RDEGEISYPE 250
DIFLTAGASA AVNYLLSIFC RGPETGVLIP IPQYPLYTAT LALNNSQALP 300
YYLDENSGWS TNPEEIETVV KEAIQNEIKP TVLVVINPGN PTGAVLSPES 350
IAQIFEVAAK YGTVVIADEV YQENIFPGTK FHSMKKILRH LQREHPGKFD 400
NVQLASLHST SKGVSGECGQ RGGYMELTGF SHEMRQVILK LASISLCPVV 450
TGQALVDLMV RPPVEGEESF ESDQAERNSI HEKLITRAMT LYETFNSLEG 500
IECQKPQGAM YLFPKIDLPF KAVQEARHLE LTPDEFYCKK LLESTGICTV 550
PGSGFGQEPG TYHLRTTFLA PGLEWIKKWE SFHKEFFDQY RD 592
Length:592
Mass (Da):66,422
Last modified:October 1, 1996 - v1
Checksum:iEE94F059BE7BAF0B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U53880 Genomic DNA. Translation: AAB67593.1.
Z73261 Genomic DNA. Translation: CAA97650.1.
BK006945 Genomic DNA. Translation: DAA09405.1.
PIRiS64923.
RefSeqiNP_013190.1. NM_001181976.1.

Genome annotation databases

EnsemblFungiiYLR089C; YLR089C; YLR089C.
GeneIDi850778.
KEGGisce:YLR089C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U53880 Genomic DNA. Translation: AAB67593.1 .
Z73261 Genomic DNA. Translation: CAA97650.1 .
BK006945 Genomic DNA. Translation: DAA09405.1 .
PIRi S64923.
RefSeqi NP_013190.1. NM_001181976.1.

3D structure databases

ProteinModelPortali P52893.
SMRi P52893. Positions 111-591.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31362. 154 interactions.
IntActi P52893. 5 interactions.
MINTi MINT-2492558.
STRINGi 4932.YLR089C.

Proteomic databases

MaxQBi P52893.
PaxDbi P52893.
PeptideAtlasi P52893.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR089C ; YLR089C ; YLR089C .
GeneIDi 850778.
KEGGi sce:YLR089C.

Organism-specific databases

CYGDi YLR089c.
SGDi S000004079. ALT1.

Phylogenomic databases

eggNOGi COG0436.
GeneTreei ENSGT00650000093331.
HOGENOMi HOG000215020.
KOi K00814.
OMAi FPANPQD.
OrthoDBi EOG7GXPMR.

Enzyme and pathway databases

UniPathwayi UPA00528 ; UER00586 .
BioCyci YEAST:YLR089C-MONOMER.

Miscellaneous databases

NextBioi 966960.

Gene expression databases

Genevestigatori P52893.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALAM_YEAST
AccessioniPrimary (citable) accession number: P52893
Secondary accession number(s): D6VY89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9960 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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