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P52893

- ALAM_YEAST

UniProt

P52893 - ALAM_YEAST

Protein

Probable alanine aminotransferase, mitochondrial

Gene

ALT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    GO - Molecular functioni

    1. L-alanine:2-oxoglutarate aminotransferase activity Source: SGD
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. alanine biosynthetic process Source: SGD
    2. alanine catabolic process Source: SGD
    3. chronological cell aging Source: SGD
    4. L-alanine catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciYEAST:YLR089C-MONOMER.
    UniPathwayiUPA00528; UER00586.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable alanine aminotransferase, mitochondrial (EC:2.6.1.2)
    Alternative name(s):
    Glutamate pyruvate transaminase
    Short name:
    GPT
    Glutamic--alanine transaminase
    Glutamic--pyruvic transaminase
    Gene namesi
    Name:ALT1
    Ordered Locus Names:YLR089C
    ORF Names:L9449.15
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR089c.
    SGDiS000004079. ALT1.

    Subcellular locationi

    Mitochondrion matrix 1 Publication

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 592Probable alanine aminotransferase, mitochondrialPRO_0000001220
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei77 – 771Phosphoserine1 Publication
    Modified residuei412 – 4121N6-(pyridoxal phosphate)lysineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP52893.
    PaxDbiP52893.
    PeptideAtlasiP52893.

    Expressioni

    Gene expression databases

    GenevestigatoriP52893.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi31362. 154 interactions.
    IntActiP52893. 5 interactions.
    MINTiMINT-2492558.
    STRINGi4932.YLR089C.

    Structurei

    3D structure databases

    ProteinModelPortaliP52893.
    SMRiP52893. Positions 111-591.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0436.
    GeneTreeiENSGT00650000093331.
    HOGENOMiHOG000215020.
    KOiK00814.
    OMAiFPANPQD.
    OrthoDBiEOG7GXPMR.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52893-1 [UniParc]FASTAAdd to Basket

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    MLSLSAKNHF TVSNSITHVI KSYHIRTLTS SAEKMPHITT PFSTSASSTK    50
    LKAFRKVRPV LQRHSSSWIV AQNHRRSLSG QSSLNDLRHL NRFPHHTLKT 100
    SNNEFYPAEQ LTLEDVNENV LKAKYAVRGA IPMRAEELKA QLEKDPQSLP 150
    FDRIINANIG NPQQLQQKPL TYYRQVLSLL QYPELLNQNE QQLVDSKLFK 200
    LDAIKRAKSL MEDIGGSVGA YSSSQGVEGI RKSVAEFITK RDEGEISYPE 250
    DIFLTAGASA AVNYLLSIFC RGPETGVLIP IPQYPLYTAT LALNNSQALP 300
    YYLDENSGWS TNPEEIETVV KEAIQNEIKP TVLVVINPGN PTGAVLSPES 350
    IAQIFEVAAK YGTVVIADEV YQENIFPGTK FHSMKKILRH LQREHPGKFD 400
    NVQLASLHST SKGVSGECGQ RGGYMELTGF SHEMRQVILK LASISLCPVV 450
    TGQALVDLMV RPPVEGEESF ESDQAERNSI HEKLITRAMT LYETFNSLEG 500
    IECQKPQGAM YLFPKIDLPF KAVQEARHLE LTPDEFYCKK LLESTGICTV 550
    PGSGFGQEPG TYHLRTTFLA PGLEWIKKWE SFHKEFFDQY RD 592
    Length:592
    Mass (Da):66,422
    Last modified:October 1, 1996 - v1
    Checksum:iEE94F059BE7BAF0B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53880 Genomic DNA. Translation: AAB67593.1.
    Z73261 Genomic DNA. Translation: CAA97650.1.
    BK006945 Genomic DNA. Translation: DAA09405.1.
    PIRiS64923.
    RefSeqiNP_013190.1. NM_001181976.1.

    Genome annotation databases

    EnsemblFungiiYLR089C; YLR089C; YLR089C.
    GeneIDi850778.
    KEGGisce:YLR089C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53880 Genomic DNA. Translation: AAB67593.1 .
    Z73261 Genomic DNA. Translation: CAA97650.1 .
    BK006945 Genomic DNA. Translation: DAA09405.1 .
    PIRi S64923.
    RefSeqi NP_013190.1. NM_001181976.1.

    3D structure databases

    ProteinModelPortali P52893.
    SMRi P52893. Positions 111-591.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31362. 154 interactions.
    IntActi P52893. 5 interactions.
    MINTi MINT-2492558.
    STRINGi 4932.YLR089C.

    Proteomic databases

    MaxQBi P52893.
    PaxDbi P52893.
    PeptideAtlasi P52893.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR089C ; YLR089C ; YLR089C .
    GeneIDi 850778.
    KEGGi sce:YLR089C.

    Organism-specific databases

    CYGDi YLR089c.
    SGDi S000004079. ALT1.

    Phylogenomic databases

    eggNOGi COG0436.
    GeneTreei ENSGT00650000093331.
    HOGENOMi HOG000215020.
    KOi K00814.
    OMAi FPANPQD.
    OrthoDBi EOG7GXPMR.

    Enzyme and pathway databases

    UniPathwayi UPA00528 ; UER00586 .
    BioCyci YEAST:YLR089C-MONOMER.

    Miscellaneous databases

    NextBioi 966960.

    Gene expression databases

    Genevestigatori P52893.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
      Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
      Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiALAM_YEAST
    AccessioniPrimary (citable) accession number: P52893
    Secondary accession number(s): D6VY89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 9960 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3