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Protein

Probable alanine aminotransferase, mitochondrial

Gene

ALT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 9960 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathwayi: L-alanine degradation via transaminase pathway

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable alanine aminotransferase (ALT2), Probable alanine aminotransferase, mitochondrial (ALT1)
This subpathway is part of the pathway L-alanine degradation via transaminase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from L-alanine, the pathway L-alanine degradation via transaminase pathway and in Amino-acid degradation.

GO - Molecular functioni

  • L-alanine:2-oxoglutarate aminotransferase activity Source: SGD
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • alanine biosynthetic process Source: SGD
  • alanine catabolic process Source: SGD
  • chronological cell aging Source: SGD
  • L-alanine catabolic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YLR089C-MONOMER.
ReactomeiR-SCE-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alanine aminotransferase, mitochondrial (EC:2.6.1.2)
Alternative name(s):
Glutamate pyruvate transaminase
Short name:
GPT
Glutamic--alanine transaminase
Glutamic--pyruvic transaminase
Gene namesi
Name:ALT1
Ordered Locus Names:YLR089C
ORF Names:L9449.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR089C.
SGDiS000004079. ALT1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000001220? – 592Probable alanine aminotransferase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77PhosphoserineCombined sources1
Modified residuei412N6-(pyridoxal phosphate)lysineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP52893.
PRIDEiP52893.

PTM databases

iPTMnetiP52893.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi31362. 400 interactors.
IntActiP52893. 5 interactors.
MINTiMINT-2492558.
STRINGi4932.YLR089C.

Structurei

3D structure databases

ProteinModelPortaliP52893.
SMRiP52893.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00650000093331.
HOGENOMiHOG000215020.
InParanoidiP52893.
KOiK00814.
OMAiVQGAMYV.
OrthoDBiEOG092C291F.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiView protein in InterPro
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_sub2.
PfamiView protein in Pfam
PF00155. Aminotran_1_2. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLSAKNHF TVSNSITHVI KSYHIRTLTS SAEKMPHITT PFSTSASSTK
60 70 80 90 100
LKAFRKVRPV LQRHSSSWIV AQNHRRSLSG QSSLNDLRHL NRFPHHTLKT
110 120 130 140 150
SNNEFYPAEQ LTLEDVNENV LKAKYAVRGA IPMRAEELKA QLEKDPQSLP
160 170 180 190 200
FDRIINANIG NPQQLQQKPL TYYRQVLSLL QYPELLNQNE QQLVDSKLFK
210 220 230 240 250
LDAIKRAKSL MEDIGGSVGA YSSSQGVEGI RKSVAEFITK RDEGEISYPE
260 270 280 290 300
DIFLTAGASA AVNYLLSIFC RGPETGVLIP IPQYPLYTAT LALNNSQALP
310 320 330 340 350
YYLDENSGWS TNPEEIETVV KEAIQNEIKP TVLVVINPGN PTGAVLSPES
360 370 380 390 400
IAQIFEVAAK YGTVVIADEV YQENIFPGTK FHSMKKILRH LQREHPGKFD
410 420 430 440 450
NVQLASLHST SKGVSGECGQ RGGYMELTGF SHEMRQVILK LASISLCPVV
460 470 480 490 500
TGQALVDLMV RPPVEGEESF ESDQAERNSI HEKLITRAMT LYETFNSLEG
510 520 530 540 550
IECQKPQGAM YLFPKIDLPF KAVQEARHLE LTPDEFYCKK LLESTGICTV
560 570 580 590
PGSGFGQEPG TYHLRTTFLA PGLEWIKKWE SFHKEFFDQY RD
Length:592
Mass (Da):66,422
Last modified:October 1, 1996 - v1
Checksum:iEE94F059BE7BAF0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53880 Genomic DNA. Translation: AAB67593.1.
Z73261 Genomic DNA. Translation: CAA97650.1.
BK006945 Genomic DNA. Translation: DAA09405.1.
PIRiS64923.
RefSeqiNP_013190.1. NM_001181976.1.

Genome annotation databases

EnsemblFungiiYLR089C; YLR089C; YLR089C.
GeneIDi850778.
KEGGisce:YLR089C.

Similar proteinsi

Entry informationi

Entry nameiALAM_YEAST
AccessioniPrimary (citable) accession number: P52893
Secondary accession number(s): D6VY89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names