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P52892

- ALAT_YEAST

UniProt

P52892 - ALAT_YEAST

Protein

Probable alanine aminotransferase

Gene

ALT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. transaminase activity Source: SGD

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. L-alanine catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciYEAST:YDR111C-MONOMER.
    UniPathwayiUPA00528; UER00586.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable alanine aminotransferase (EC:2.6.1.2)
    Alternative name(s):
    Glutamate pyruvate transaminase
    Short name:
    GPT
    Glutamic--alanine transaminase
    Glutamic--pyruvic transaminase
    Gene namesi
    Name:ALT2
    Ordered Locus Names:YDR111C
    ORF Names:YD9727.07C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR111c.
    SGDiS000002518. ALT2.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 507507Probable alanine aminotransferasePRO_0000123937Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei327 – 3271N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    MaxQBiP52892.
    PaxDbiP52892.

    Expressioni

    Gene expression databases

    GenevestigatoriP52892.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi32169. 30 interactions.
    DIPiDIP-4931N.
    IntActiP52892. 2 interactions.
    MINTiMINT-550889.
    STRINGi4932.YDR111C.

    Structurei

    3D structure databases

    ProteinModelPortaliP52892.
    SMRiP52892. Positions 30-506.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0436.
    GeneTreeiENSGT00650000093331.
    HOGENOMiHOG000215020.
    KOiK00814.
    OMAiEESNWST.
    OrthoDBiEOG7GXPMR.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P52892-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTMTHQQDLK GVFTAKDLDF KPAGKITKKD LNTGVTKAEY AVRGAIPTRA    50
    DELKEELKKN PEVLPFDDII NANIGNPQQL DQKPLTFTRQ VLAILEYPEI 100
    LRVGHNELAS LNLFSRDALE RAERLLNDIG GSIGAYSHSQ GVPGIRQTVA 150
    DFITRRDGGE PATPEDIYLT TGASSAATSL LSLLCKDSQT GLLIPIPQYP 200
    LYTASASLFN AQVLPYYLDE ESNWSTNSDE IEKVVQDALK KQIRPSVLIV 250
    INPGNPTGAV LSEETIARIC LIAAKYGITI ISDEVYQENI FNDVKFHSMK 300
    KVLRKLQHLY PGKFDNVQLA SLHSISKGFM DECGQRGGYM EIIGFSQEIR 350
    DALFKLMSIS ICSVVTGQAV VDLMVKPPQP GDESYEQDHD ERLKIFHEMR 400
    TRANLLYETF KELEGIECQK PQGAMYLFPR LVLPKKALCE SERLGIEPDE 450
    FYCTSLLEST GICTVPGSGF GQRPGTYHVR TTFLAPGTKW IQDWKEFHQD 500
    FFSKYRN 507
    Length:507
    Mass (Da):56,770
    Last modified:October 1, 1996 - v1
    Checksum:iCB4DCD137CF26085
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti420 – 4201K → E in AAU09694. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48758 Genomic DNA. Translation: CAA88665.1.
    AY723777 Genomic DNA. Translation: AAU09694.1.
    BK006938 Genomic DNA. Translation: DAA11957.1.
    PIRiS52677.
    RefSeqiNP_010396.1. NM_001180419.1.

    Genome annotation databases

    EnsemblFungiiYDR111C; YDR111C; YDR111C.
    GeneIDi851690.
    KEGGisce:YDR111C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48758 Genomic DNA. Translation: CAA88665.1 .
    AY723777 Genomic DNA. Translation: AAU09694.1 .
    BK006938 Genomic DNA. Translation: DAA11957.1 .
    PIRi S52677.
    RefSeqi NP_010396.1. NM_001180419.1.

    3D structure databases

    ProteinModelPortali P52892.
    SMRi P52892. Positions 30-506.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32169. 30 interactions.
    DIPi DIP-4931N.
    IntActi P52892. 2 interactions.
    MINTi MINT-550889.
    STRINGi 4932.YDR111C.

    Proteomic databases

    MaxQBi P52892.
    PaxDbi P52892.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR111C ; YDR111C ; YDR111C .
    GeneIDi 851690.
    KEGGi sce:YDR111C.

    Organism-specific databases

    CYGDi YDR111c.
    SGDi S000002518. ALT2.

    Phylogenomic databases

    eggNOGi COG0436.
    GeneTreei ENSGT00650000093331.
    HOGENOMi HOG000215020.
    KOi K00814.
    OMAi EESNWST.
    OrthoDBi EOG7GXPMR.

    Enzyme and pathway databases

    UniPathwayi UPA00528 ; UER00586 .
    BioCyci YEAST:YDR111C-MONOMER.

    Miscellaneous databases

    NextBioi 969341.

    Gene expression databases

    Genevestigatori P52892.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiALAT_YEAST
    AccessioniPrimary (citable) accession number: P52892
    Secondary accession number(s): D6VS97, Q66RF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3480 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3