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Protein

Probable alanine aminotransferase

Gene

ALT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 3480 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathwayi: L-alanine degradation via transaminase pathway

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable alanine aminotransferase (ALT2), Probable alanine aminotransferase, mitochondrial (ALT1)
This subpathway is part of the pathway L-alanine degradation via transaminase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from L-alanine, the pathway L-alanine degradation via transaminase pathway and in Amino-acid degradation.

GO - Molecular functioni

  • pyridoxal phosphate binding Source: InterPro
  • transaminase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YDR111C-MONOMER
ReactomeiR-SCE-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00528; UER00586

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alanine aminotransferase (EC:2.6.1.2)
Alternative name(s):
Glutamate pyruvate transaminase
Short name:
GPT
Glutamic--alanine transaminase
Glutamic--pyruvic transaminase
Gene namesi
Name:ALT2
Ordered Locus Names:YDR111C
ORF Names:YD9727.07C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR111C
SGDiS000002518 ALT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001239371 – 507Probable alanine aminotransferaseAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei327N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

MaxQBiP52892
PaxDbiP52892
PRIDEiP52892

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi32169, 65 interactors
DIPiDIP-4931N
IntActiP52892, 2 interactors
MINTiP52892
STRINGi4932.YDR111C

Structurei

3D structure databases

ProteinModelPortaliP52892
SMRiP52892
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00650000093331
HOGENOMiHOG000215020
InParanoidiP52892
KOiK00814
OMAiQMLKLMS
OrthoDBiEOG092C291F

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequencei

Sequence statusi: Complete.

P52892-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMTHQQDLK GVFTAKDLDF KPAGKITKKD LNTGVTKAEY AVRGAIPTRA
60 70 80 90 100
DELKEELKKN PEVLPFDDII NANIGNPQQL DQKPLTFTRQ VLAILEYPEI
110 120 130 140 150
LRVGHNELAS LNLFSRDALE RAERLLNDIG GSIGAYSHSQ GVPGIRQTVA
160 170 180 190 200
DFITRRDGGE PATPEDIYLT TGASSAATSL LSLLCKDSQT GLLIPIPQYP
210 220 230 240 250
LYTASASLFN AQVLPYYLDE ESNWSTNSDE IEKVVQDALK KQIRPSVLIV
260 270 280 290 300
INPGNPTGAV LSEETIARIC LIAAKYGITI ISDEVYQENI FNDVKFHSMK
310 320 330 340 350
KVLRKLQHLY PGKFDNVQLA SLHSISKGFM DECGQRGGYM EIIGFSQEIR
360 370 380 390 400
DALFKLMSIS ICSVVTGQAV VDLMVKPPQP GDESYEQDHD ERLKIFHEMR
410 420 430 440 450
TRANLLYETF KELEGIECQK PQGAMYLFPR LVLPKKALCE SERLGIEPDE
460 470 480 490 500
FYCTSLLEST GICTVPGSGF GQRPGTYHVR TTFLAPGTKW IQDWKEFHQD

FFSKYRN
Length:507
Mass (Da):56,770
Last modified:October 1, 1996 - v1
Checksum:iCB4DCD137CF26085
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti420K → E in AAU09694 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48758 Genomic DNA Translation: CAA88665.1
AY723777 Genomic DNA Translation: AAU09694.1
BK006938 Genomic DNA Translation: DAA11957.1
PIRiS52677
RefSeqiNP_010396.1, NM_001180419.1

Genome annotation databases

EnsemblFungiiYDR111C; YDR111C; YDR111C
GeneIDi851690
KEGGisce:YDR111C

Similar proteinsi

Entry informationi

Entry nameiALAT_YEAST
AccessioniPrimary (citable) accession number: P52892
Secondary accession number(s): D6VS97, Q66RF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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