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Protein

Probable alanine aminotransferase

Gene

ALT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathway: L-alanine degradation via transaminase pathway

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable alanine aminotransferase (ALT2), Probable alanine aminotransferase, mitochondrial (ALT1)
This subpathway is part of the pathway L-alanine degradation via transaminase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from L-alanine, the pathway L-alanine degradation via transaminase pathway and in Amino-acid degradation.

GO - Molecular functioni

  • pyridoxal phosphate binding Source: InterPro
  • transaminase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YDR111C-MONOMER.
ReactomeiREACT_288999. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alanine aminotransferase (EC:2.6.1.2)
Alternative name(s):
Glutamate pyruvate transaminase
Short name:
GPT
Glutamic--alanine transaminase
Glutamic--pyruvic transaminase
Gene namesi
Name:ALT2
Ordered Locus Names:YDR111C
ORF Names:YD9727.07C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR111c.
EuPathDBiFungiDB:YDR111C.
SGDiS000002518. ALT2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507Probable alanine aminotransferasePRO_0000123937Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei327 – 3271N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

MaxQBiP52892.
PaxDbiP52892.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi32169. 31 interactions.
DIPiDIP-4931N.
IntActiP52892. 2 interactions.
MINTiMINT-550889.
STRINGi4932.YDR111C.

Structurei

3D structure databases

ProteinModelPortaliP52892.
SMRiP52892. Positions 30-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0436.
GeneTreeiENSGT00650000093331.
HOGENOMiHOG000215020.
InParanoidiP52892.
KOiK00814.
OMAiTIARICL.
OrthoDBiEOG7GXPMR.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

P52892-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMTHQQDLK GVFTAKDLDF KPAGKITKKD LNTGVTKAEY AVRGAIPTRA
60 70 80 90 100
DELKEELKKN PEVLPFDDII NANIGNPQQL DQKPLTFTRQ VLAILEYPEI
110 120 130 140 150
LRVGHNELAS LNLFSRDALE RAERLLNDIG GSIGAYSHSQ GVPGIRQTVA
160 170 180 190 200
DFITRRDGGE PATPEDIYLT TGASSAATSL LSLLCKDSQT GLLIPIPQYP
210 220 230 240 250
LYTASASLFN AQVLPYYLDE ESNWSTNSDE IEKVVQDALK KQIRPSVLIV
260 270 280 290 300
INPGNPTGAV LSEETIARIC LIAAKYGITI ISDEVYQENI FNDVKFHSMK
310 320 330 340 350
KVLRKLQHLY PGKFDNVQLA SLHSISKGFM DECGQRGGYM EIIGFSQEIR
360 370 380 390 400
DALFKLMSIS ICSVVTGQAV VDLMVKPPQP GDESYEQDHD ERLKIFHEMR
410 420 430 440 450
TRANLLYETF KELEGIECQK PQGAMYLFPR LVLPKKALCE SERLGIEPDE
460 470 480 490 500
FYCTSLLEST GICTVPGSGF GQRPGTYHVR TTFLAPGTKW IQDWKEFHQD

FFSKYRN
Length:507
Mass (Da):56,770
Last modified:October 1, 1996 - v1
Checksum:iCB4DCD137CF26085
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti420 – 4201K → E in AAU09694 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48758 Genomic DNA. Translation: CAA88665.1.
AY723777 Genomic DNA. Translation: AAU09694.1.
BK006938 Genomic DNA. Translation: DAA11957.1.
PIRiS52677.
RefSeqiNP_010396.1. NM_001180419.1.

Genome annotation databases

EnsemblFungiiYDR111C; YDR111C; YDR111C.
GeneIDi851690.
KEGGisce:YDR111C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48758 Genomic DNA. Translation: CAA88665.1.
AY723777 Genomic DNA. Translation: AAU09694.1.
BK006938 Genomic DNA. Translation: DAA11957.1.
PIRiS52677.
RefSeqiNP_010396.1. NM_001180419.1.

3D structure databases

ProteinModelPortaliP52892.
SMRiP52892. Positions 30-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32169. 31 interactions.
DIPiDIP-4931N.
IntActiP52892. 2 interactions.
MINTiMINT-550889.
STRINGi4932.YDR111C.

Proteomic databases

MaxQBiP52892.
PaxDbiP52892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR111C; YDR111C; YDR111C.
GeneIDi851690.
KEGGisce:YDR111C.

Organism-specific databases

CYGDiYDR111c.
EuPathDBiFungiDB:YDR111C.
SGDiS000002518. ALT2.

Phylogenomic databases

eggNOGiCOG0436.
GeneTreeiENSGT00650000093331.
HOGENOMiHOG000215020.
InParanoidiP52892.
KOiK00814.
OMAiTIARICL.
OrthoDBiEOG7GXPMR.

Enzyme and pathway databases

UniPathwayiUPA00528; UER00586.
BioCyciYEAST:YDR111C-MONOMER.
ReactomeiREACT_288999. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

NextBioi969341.
PROiP52892.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALAT_YEAST
AccessioniPrimary (citable) accession number: P52892
Secondary accession number(s): D6VS97, Q66RF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3480 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.