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Protein

Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

C08H9.3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-CEL-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
ORF Names:C08H9.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiC08H9.3a; CE47030; WBGene00007464.
C08H9.3b; CE37608; WBGene00007464.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence analysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence analysisAdd
BLAST
Transmembranei96 – 11621HelicalSequence analysisAdd
BLAST
Transmembranei156 – 17621HelicalSequence analysisAdd
BLAST
Transmembranei190 – 21021HelicalSequence analysisAdd
BLAST
Transmembranei228 – 24821HelicalSequence analysisAdd
BLAST
Transmembranei324 – 34421HelicalSequence analysisAdd
BLAST
Transmembranei350 – 37021HelicalSequence analysisAdd
BLAST
Transmembranei395 – 41521HelicalSequence analysisAdd
BLAST
Transmembranei423 – 44321HelicalSequence analysisAdd
BLAST
Transmembranei452 – 47221HelicalSequence analysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 766766Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferasePRO_0000174164Add
BLAST

Proteomic databases

PaxDbiP52887.
PRIDEiP52887.

Expressioni

Gene expression databases

BgeeiWBGene00007464.

Interactioni

Protein-protein interaction databases

BioGridi39864. 1 interaction.
STRINGi6239.C08H9.3a.

Structurei

3D structure databases

ProteinModelPortaliP52887.
SMRiP52887. Positions 514-758.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2576. Eukaryota.
KOG4417. Eukaryota.
ENOG410XPPM. LUCA.
GeneTreeiENSGT00390000011880.
HOGENOMiHOG000016206.
InParanoidiP52887.
KOiK03849.
OMAiLVQEYSH.
OrthoDBiEOG091G04J6.

Family and domain databases

CDDicd06559. Endonuclease_V. 1 hit.
InterProiIPR021173. Dolichyl-P-Glc/endonucV.
IPR007581. Endonuclease-V.
IPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
PF04493. Endonuclease_5. 1 hit.
[Graphical view]
PIRSFiPIRSF037345. Dolichyl-P-Glc/endonucV. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P52887-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEVQLVLAV TAILISFKCL LIPAYVSTDF EVHRNWMAVT WQRPLCEWYT
60 70 80 90 100
EATSEWTLDY PPFFAYFELG LASVAHFFGF DECLVISKTP RFSRRILIFQ
110 120 130 140 150
RFSVIFCDIL YIAVCALYSF RSPRLVSRIP KKLQQNGREA CFVLLASLQA
160 170 180 190 200
LIICDSIHFQ YNSMLTAIFL MSLFFIDTER YLMAALSYSI LLNFKHIYVY
210 220 230 240 250
YALGYVFYYL VNYFQFSGNV LLANTPKAIS LAIALLIPFC ASIFPFIHAS
260 270 280 290 300
GVQGLQNIAT RLFPVSRGLT HAYWAPNFWA LYNFADLCLY RVLSLLKIGK
310 320 330 340 350
FDAPTYTSGL VQEYSHSVLP NVSPMGTLCL VVISSMIVLT GLVIRRKDSA
360 370 380 390 400
DFSLFAVFSA FCFFYFGYHV HEKAIILVTV PMTVFAIKNP KYHSILIHLT
410 420 430 440 450
CIASFSLFPL LFTPFETLLK YAICVSYFFI QLVFLKRVTL MPLSDLIPTR
460 470 480 490 500
HVASWLLMGM VEVYNTFLHK WLWTSRLPFA PLMAISILTA IELTGLIGAL
510 520 530 540 550
IWSTFGDGIF EIWWAKATCQ IRERLIRDST YSVQAVEDLD DVKLVAGIDT
560 570 580 590 600
SAAKLNSDMV YISVSFWTYP DLKHVATISD TRMLELPYIP QYLAVREAEV
610 620 630 640 650
MADFLKSVIT ERPELRPDVI LCDGFGEFHS RGCGMACHVG ALSGIASIGV
660 670 680 690 700
AKNLTLHHTY ETIGMENKSK VDSFVEHCRE VYKNNKTSPG FIPFDIVEPV
710 720 730 740 750
VLNILRMGSS MNGVFVSAGY GIDLELSTEI CSQLLLNNTT IEPIRAADLE
760
SRRLVRENFD GNEKLE
Note: No experimental confirmation available.
Length:766
Mass (Da):86,870
Last modified:October 3, 2012 - v3
Checksum:iB335E2AD83D90934
GO
Isoform b (identifier: P52887-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     111-123: YIAVCALYSFRSP → LLSALYTPSALHV
     124-766: Missing.

Note: No experimental confirmation available.
Show »
Length:123
Mass (Da):14,252
Checksum:i1B2BCB1492090259
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei111 – 12313YIAVC…SFRSP → LLSALYTPSALHV in isoform b. CuratedVSP_012307Add
BLAST
Alternative sequencei124 – 766643Missing in isoform b. CuratedVSP_012308Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54342 Genomic DNA. Translation: CAA91145.3.
Z54342 Genomic DNA. Translation: CAH60747.1.
PIRiT19107.
RefSeqiNP_001021940.2. NM_001026769.3. [P52887-1]
NP_001021941.1. NM_001026770.3. [P52887-2]
UniGeneiCel.15926.

Genome annotation databases

EnsemblMetazoaiC08H9.3a; C08H9.3a; WBGene00007464. [P52887-1]
GeneIDi174542.
KEGGicel:CELE_C08H9.3.
UCSCiC08H9.3a. c. elegans. [P52887-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54342 Genomic DNA. Translation: CAA91145.3.
Z54342 Genomic DNA. Translation: CAH60747.1.
PIRiT19107.
RefSeqiNP_001021940.2. NM_001026769.3. [P52887-1]
NP_001021941.1. NM_001026770.3. [P52887-2]
UniGeneiCel.15926.

3D structure databases

ProteinModelPortaliP52887.
SMRiP52887. Positions 514-758.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi39864. 1 interaction.
STRINGi6239.C08H9.3a.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Proteomic databases

PaxDbiP52887.
PRIDEiP52887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC08H9.3a; C08H9.3a; WBGene00007464. [P52887-1]
GeneIDi174542.
KEGGicel:CELE_C08H9.3.
UCSCiC08H9.3a. c. elegans. [P52887-1]

Organism-specific databases

CTDi174542.
WormBaseiC08H9.3a; CE47030; WBGene00007464.
C08H9.3b; CE37608; WBGene00007464.

Phylogenomic databases

eggNOGiKOG2576. Eukaryota.
KOG4417. Eukaryota.
ENOG410XPPM. LUCA.
GeneTreeiENSGT00390000011880.
HOGENOMiHOG000016206.
InParanoidiP52887.
KOiK03849.
OMAiLVQEYSH.
OrthoDBiEOG091G04J6.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-CEL-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiP52887.

Gene expression databases

BgeeiWBGene00007464.

Family and domain databases

CDDicd06559. Endonuclease_V. 1 hit.
InterProiIPR021173. Dolichyl-P-Glc/endonucV.
IPR007581. Endonuclease-V.
IPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
PF04493. Endonuclease_5. 1 hit.
[Graphical view]
PIRSFiPIRSF037345. Dolichyl-P-Glc/endonucV. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiALG8_CAEEL
AccessioniPrimary (citable) accession number: P52887
Secondary accession number(s): Q5WRQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2012
Last modified: September 7, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.