Reviewed, UniProtKB/Swiss-Prot P52887 (ALG8_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
    Asparagine-linked glycosylation protein 8

Gene names

ORF Names:

C08H9.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG6/ALG8 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: InterPro embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	endonuclease activity Inferred from electronic annotation. Source: InterPro transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P52887-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: P52887-2)

The sequence of this isoform differs from the canonical sequence as follows:
     111-123: YIAVCALYSFRSP → LLSALYTPSALHV
     124-758: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase		PRO_0000174164
Regions
	Transmembrane	6 – 26	21	Potential
	Transmembrane	60 – 80	21	Potential
	Transmembrane	96 – 116	21	Potential
	Transmembrane	156 – 176	21	Potential
	Transmembrane	190 – 210	21	Potential
	Transmembrane	229 – 249	21	Potential
	Transmembrane	324 – 344	21	Potential
	Transmembrane	350 – 370	21	Potential
	Transmembrane	395 – 415	21	Potential
	Transmembrane	423 – 443	21	Potential
	Transmembrane	474 – 494	21	Potential
Natural variations
	Alternative sequence	111 – 123	13	YIAVC…SFRSP → LLSALYTPSALHV in isoform b.		VSP_012307
	Alternative sequence	124 – 758	635	Missing in isoform b.		VSP_012308

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified December 21, 2004. Version 2. Checksum: 48964311CEC6DF6F	FASTA	758	85,867
	10 20 30 40 50 60 MGEVQLVLAV TAILISFKCL LIPAYVSTDF EVHRNWMAVT WQRPLCEWYT EATSEWTLDY 70 80 90 100 110 120 PPFFAYFELG LASVAHFFGF DECLVISKTP RFSRRILIFQ RFSVIFCDIL YIAVCALYSF 130 140 150 160 170 180 RSPRLVSRIP KKLQQNGREA CFVLLASLQA LIICDSIHFQ YNSMLTAIFL MSLFFIDTER 190 200 210 220 230 240 YLMAALSYSI LLNFKHIYVY YALGYVFYYL VNYFQFSGNV LLANTPKAIS LAIALLIPFC 250 260 270 280 290 300 ASIFPFIHAS GVQGLQNIAT RLFPVSRGLT HAYWAPNFWA LYNFADLCLY RVLSLLKIGK 310 320 330 340 350 360 FDAPTYTSGL VQEYSHSVLP NVSPMGTLCL VVISSMIVLT GLVIRRKDSA DFSLFAVFSA 370 380 390 400 410 420 FCFFYFGYHV HEKAIILVTV PMTVFAIKNP KYHSILIHLT CIASFSLFPL LFTPFETLLK 430 440 450 460 470 480 YAICVSYFFI QLVFLKRVTL MPLSDLIPTR HVASWLLMGM VEWLWTSRLP FAPLMAISIL 490 500 510 520 530 540 TAIELTGLIG ALIWSTFGDG IFEIWWAKAT CQIRERLIRD STYSVQAVED LDDVKLVAGI 550 560 570 580 590 600 DTSAAKLNSD MVYISVSFWT YPDLKHVATI SDTRMLELPY IPQYLAVREA EVMADFLKSV 610 620 630 640 650 660 ITERPELRPD VILCDGFGEF HSRGCGMACH VGALSGIASI GVAKNLTLHH TYETIGMENK 670 680 690 700 710 720 SKVDSFVEHC REVYKNNKTS PGFIPFDIVE PVVLNILRMG SSMNGVFVSA GYGIDLELST 730 740 750 EICSQLLLNN TTIEPIRAAD LESRRLVREN FDGNEKLE
	Isoform b. Checksum: 1B2BCB1492090259	FASTA	123	14,252
	10 20 30 40 50 60 MGEVQLVLAV TAILISFKCL LIPAYVSTDF EVHRNWMAVT WQRPLCEWYT EATSEWTLDY 70 80 90 100 110 120 PPFFAYFELG LASVAHFFGF DECLVISKTP RFSRRILIFQ RFSVIFCDIL LLSALYTPSA LHV

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z54342 Genomic DNA. Translation: CAA91145.2. Z54342 Genomic DNA. Translation: CAH60747.1.
PIR	T19107.
RefSeq	NP_001021940.1.
UniGene	Cel.15926
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT57. Glycosyltransferase Family 57.
Proteomic databases
PRIDE	P52887.
Genome annotation databases
Ensembl	C08H9.3. Caenorhabditis elegans. [Contig view]
GeneID	174542.
KEGG	cel:C08H9.3.
NMPDR	fig|6239.3.peg.7064.
Organism-specific databases
WormBase	WBGene00007464. C08H9.3.
WormPep	C08H9.3a. CE36914. [WorfDB] C08H9.3b. CE37608. [WorfDB]
Phylogenomic databases
OMA	P52887. NIATRLF.
Gene expression databases
ArrayExpress	P52887.
Family and domain databases
InterPro	IPR007581. Endonuclease-V. IPR004856. Glycosyltransferase_ALG6/ALG8. [Graphical view]
PANTHER	PTHR12413. Alg6_Alg8. 1 hit.
Pfam	PF03155. Alg6_Alg8. 1 hit. PF04493. Endonuclease_5. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	884478.

Entry information

Entry name

ALG8_CAEEL

Accession

Primary (citable) accession number: P52887
Secondary accession number(s): Q5WRQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 21, 2004
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project