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P52887 (ALG8_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
EC=2.4.1.265
Alternative name(s):
Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene names
ORF Names:C08H9.3
OrganismCaenorhabditis elegans
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length758 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity.

Catalytic activity

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the ALG6/ALG8 glucosyltransferase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: P52887-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform b (identifier: P52887-2)

The sequence of this isoform differs from the canonical sequence as follows:
     111-123: YIAVCALYSFRSP → LLSALYTPSALHV
     124-758: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 758758Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
PRO_0000174164

Regions

Transmembrane6 – 2621Helical; Potential
Transmembrane60 – 8021Helical; Potential
Transmembrane96 – 11621Helical; Potential
Transmembrane156 – 17621Helical; Potential
Transmembrane190 – 21021Helical; Potential
Transmembrane229 – 24921Helical; Potential
Transmembrane324 – 34421Helical; Potential
Transmembrane350 – 37021Helical; Potential
Transmembrane395 – 41521Helical; Potential
Transmembrane423 – 44321Helical; Potential
Transmembrane474 – 49421Helical; Potential

Natural variations

Alternative sequence111 – 12313YIAVC…SFRSP → LLSALYTPSALHV in isoform b.
VSP_012307
Alternative sequence124 – 758635Missing in isoform b.
VSP_012308

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified December 21, 2004. Version 2.
Checksum: 48964311CEC6DF6F

FASTA75885,867
        10         20         30         40         50         60 
MGEVQLVLAV TAILISFKCL LIPAYVSTDF EVHRNWMAVT WQRPLCEWYT EATSEWTLDY 

        70         80         90        100        110        120 
PPFFAYFELG LASVAHFFGF DECLVISKTP RFSRRILIFQ RFSVIFCDIL YIAVCALYSF 

       130        140        150        160        170        180 
RSPRLVSRIP KKLQQNGREA CFVLLASLQA LIICDSIHFQ YNSMLTAIFL MSLFFIDTER 

       190        200        210        220        230        240 
YLMAALSYSI LLNFKHIYVY YALGYVFYYL VNYFQFSGNV LLANTPKAIS LAIALLIPFC 

       250        260        270        280        290        300 
ASIFPFIHAS GVQGLQNIAT RLFPVSRGLT HAYWAPNFWA LYNFADLCLY RVLSLLKIGK 

       310        320        330        340        350        360 
FDAPTYTSGL VQEYSHSVLP NVSPMGTLCL VVISSMIVLT GLVIRRKDSA DFSLFAVFSA 

       370        380        390        400        410        420 
FCFFYFGYHV HEKAIILVTV PMTVFAIKNP KYHSILIHLT CIASFSLFPL LFTPFETLLK 

       430        440        450        460        470        480 
YAICVSYFFI QLVFLKRVTL MPLSDLIPTR HVASWLLMGM VEWLWTSRLP FAPLMAISIL 

       490        500        510        520        530        540 
TAIELTGLIG ALIWSTFGDG IFEIWWAKAT CQIRERLIRD STYSVQAVED LDDVKLVAGI 

       550        560        570        580        590        600 
DTSAAKLNSD MVYISVSFWT YPDLKHVATI SDTRMLELPY IPQYLAVREA EVMADFLKSV 

       610        620        630        640        650        660 
ITERPELRPD VILCDGFGEF HSRGCGMACH VGALSGIASI GVAKNLTLHH TYETIGMENK 

       670        680        690        700        710        720 
SKVDSFVEHC REVYKNNKTS PGFIPFDIVE PVVLNILRMG SSMNGVFVSA GYGIDLELST 

       730        740        750 
EICSQLLLNN TTIEPIRAAD LESRRLVREN FDGNEKLE

« Hide

Isoform b [UniParc].

Checksum: 1B2BCB1492090259
Show »

FASTA12314,252

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z54342 Genomic DNA. Translation: CAA91145.2.
Z54342 Genomic DNA. Translation: CAH60747.1.
PIRT19107.
RefSeqNP_001021940.1. NM_001026769.2.
NP_001021941.1. NM_001026770.2.
UniGeneCel.15926.

3D structure databases

ProteinModelPortalP52887.
SMRP52887. Positions 506-751.
ModBaseSearch...

Protein-protein interaction databases

STRINGP52887.

Protein family/group databases

CAZyGT57. Glycosyltransferase Family 57.

Proteomic databases

PRIDEP52887.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC08H9.3a; C08H9.3a; C08H9.3.
GeneID174542.
KEGGcel:C08H9.3.
NMPDRfig|6239.3.peg.7064.
UCSCC08H9.3a. c. elegans.

Organism-specific databases

CTD174542.
WormBaseC08H9.3a; CE36914; WBGene00007464.
C08H9.3b; CE37608; WBGene00007464.

Phylogenomic databases

eggNOGmeNOG04131.
GeneTreeEMGT00050000012559.
HOGENOMHBG381092.
InParanoidP52887.
OMAPLMAISV.
PhylomeDBP52887.

Gene expression databases

ArrayExpressP52887.

Family and domain databases

InterProIPR021173. Dolichyl-P-Glc/endonucV.
IPR007581. Endonuclease-V.
IPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
KOK03849.
PANTHERPTHR12413. Alg6_Alg8. 1 hit.
PfamPF03155. Alg6_Alg8. 1 hit.
PF04493. Endonuclease_5. 1 hit.
[Graphical view]
PIRSFPIRSF037345. Dolichyl-P-Glc/endonucV. 1 hit.
ProtoNetSearch...

Other

NextBio884478.

Entry information

Entry nameALG8_CAEEL
AccessionPrimary (citable) accession number: P52887
Secondary accession number(s): Q5WRQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 21, 2004
Last modified: November 16, 2011
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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