P52872 (DAD1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit dad-1
Short name=Oligosaccharyl transferase subunit dad-1
EC=2.4.1.119

Alternative name(s):
Defender against cell death 1
Short name=Protein dad-1

Gene names

Name:	dad-1
ORF Names:	F57B10.10

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity. Possesses cell death-inhibiting activity. Suppresses some programmed cell death in C.elegans.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the DAD/OST2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase negative regulation of apoptotic process Inferred from mutant phenotype Ref.1. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase oogenesis Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase receptor-mediated endocytosis Inferred from mutant phenotype. Source: WormBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 113	113	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit dad-1		PRO_0000124017
Regions
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	93 – 113	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P52872 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 933057F18CBB6DE6	FASTA	113	12,747
	10 20 30 40 50 60 MAAQVVPVLS KLFDDYQKTT SSKLKIIDAY MTYILFTGIF QFIYCLLVGT FPFNSFLSGF 70 80 90 100 110 ISTVTSFVLA SCLRMQVNQE NRSEFTAVST ERAFADFIFA NLILHLVVVN FLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"dad-1, an endogenous programmed cell death suppressor in Caenorhabditis elegans and vertebrates." Sugimoto A., Hozak R.R., Nakashima T., Nishimoto T., Rothman J.H. EMBO J. 14:4434-4441(1995) [PubMed: 7556086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89080 mRNA. Translation: CAA61451.1. FO081416 Genomic DNA. Translation: CCD71478.1.
PIR	S59116.
RefSeq	NP_491889.1. NM_059488.5.
UniGene	Cel.19725.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24414N.
IntAct	P52872. 1 interaction.
MINT	MINT-1129927.
STRING	P52872.
Proteomic databases
PRIDE	P52872.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F57B10.10; F57B10.10; F57B10.10.
GeneID	172370.
KEGG	cel:F57B10.10.
UCSC	F57B10.10.2. c. elegans.
Organism-specific databases
CTD	172370.
WormBase	F57B10.10; CE11316; WBGene00000896; dad-1.
Phylogenomic databases
eggNOG	meNOG20347.
GeneTree	EMGT00050000017603.
HOGENOM	HBG329225.
InParanoid	P52872.
OMA	YCCLVGT.
PhylomeDB	P52872.
Gene expression databases
ArrayExpress	P52872.
Family and domain databases
InterPro	IPR003038. DAD. [Graphical view]
KO	K12668.
PANTHER	PTHR10705. DAD. 1 hit.
Pfam	PF02109. DAD. 1 hit. [Graphical view]
PIRSF	PIRSF005588. DAD. 1 hit.
ProtoNet	Search...
Other
NextBio	875237.

Entry information

Entry name

DAD1_CAEEL

Accession

Primary (citable) accession number: P52872

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project