P52871 (SC6B2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein transport protein SBH2

Alternative name(s):
Ssh1 complex subunit SBH2
Ssh1 complex subunit beta

Gene names

Name:	SBH2
Synonyms:	SEB2
Ordered Locus Names:	YER019C-A
ORF Names:	YER019BC

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	88 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import.
Subunit structure	Component of the heterotrimeric Ssh1 complex, which is composed of SSH1, SBH2 and SSS1.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein.
Miscellaneous	Present with 414 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SEC61-beta family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Inferred from genetic interaction PubMed 18347066. Source: SGD
Cellular_component	Ssh1 translocon complex Inferred from direct assay Ref.6. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ARF guanyl-nucleotide exchange factor activity Inferred from direct assay PubMed 12750387. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 88	88	Protein transport protein SBH2		PRO_0000157262
Regions
	Topological domain	1 – 61	61	Cytoplasmic Potential
	Transmembrane	62 – 82	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P52871 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 5A4F5B9184202E88	FASTA	88	9,606
	10 20 30 40 50 60 MAASVPPGGQ RILQKRRQAQ SIKEKQAKQT PTSTRQAGYG GSSSSILKLY TDEANGFRVD 70 80 SLVVLFLSVG FIFSVIALHL LTKFTHII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast protein translocation complex: isolation of two genes SEB1 and SEB2 encoding proteins homologous to the Sec61 beta subunit." Toikkanen J., Gatti E., Takei K., Saloheimo M., Olkkonen V.M., Soederlund H., de Camilli P., Keraenen S. Yeast 12:425-438(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Yeast Sec61 beta subunit." Finke K., Panzner S., Sommer T., Hartmann E., Rapoport T.A. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae." Finke K., Plath K., Panzner S., Prehn S., Rapoport T.A., Hartmann E., Sommer T. EMBO J. 15:1482-1494(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-32.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z50012 mRNA. Translation: CAA90305.1.
L38891 mRNA. Translation: AAT01101.1.
U18778 Genomic DNA. Translation: AAB64567.1.
AY693199 Genomic DNA. Translation: AAT93218.1.
BK006939 Genomic DNA. Translation: DAA07672.1.

PIR

S68163.

RefSeq

NP_010936.1. NM_001180031.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WW9	electron microscopy	8.60	C	1-87	[»]
2WWA	electron microscopy	8.90	C	1-87	[»]

ProteinModelPortal

P52871.

SMR

P52871. Positions 52-86.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1708N.

IntAct

P52871. 6 interactions.

MINT

MINT-396359.

STRING

4932.YER019C-A.

Protein family/group databases

TCDB

3.A.5.8.1. general secretory pathway (Sec) family.

Proteomic databases

PaxDb

P52871.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YER019C-A; YER019C-A; YER019C-A.

GeneID

856740.

KEGG

sce:YER019C-A.

Organism-specific databases

CYGD

YER019c-a.

SGD

S000002127. SBH2.

Phylogenomic databases

eggNOG

NOG330120.

GeneTree

ENSGT00390000015774.

HOGENOM

HOG000211109.

KO

K09481.

OMA

YTDEANG.

OrthoDB

EOG4SXRPB.

Gene expression databases

Genevestigator

P52871.

GermOnline

YER019C-A. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005609. Sec61_beta.
IPR016482. Sec61_beta_euk.
[Graphical view]

PANTHER

PTHR13509. PTHR13509. 1 hit.

Pfam

PF03911. Sec61_beta. 1 hit.
[Graphical view]

PIRSF

PIRSF006398. Sec61_beta_euk. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P52871.

NextBio

982870.

Entry information

Entry name

SC6B2_YEAST

Accession

Primary (citable) accession number: P52871
Secondary accession number(s): D3DLR8, Q53X22

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program