P52870 (SC6B1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein transport protein SBH1

Alternative name(s):
Sec61 complex subunit SBH1
Sec61 complex subunit beta

Gene names

Name:	SBH1
Synonyms:	SEB1
Ordered Locus Names:	YER087C-B
ORF Names:	YER087BC

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	82 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the Sec61 complex, which is the major component of a channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). The functional states of the translocon complex include co- and post-translational ER import, cotranslational membrane protein integration and retrograde transport of misfolded proteins out of the ER. In the cotranslational pathway, ribosomes synthesizing presecretory proteins are targeted to the translocon by the cytosolic signal recognition particle (SRP) and its ER-localized receptor. The association of the Sec61 complex with the ribosome is mediated by the 28S rRNA of the large ribosomal subunit. SRP-independent post-translational translocation requires the association of additional factors, such as the Sec62/63 complex and KAR2.
Subunit structure	Component of the heterotrimeric Sec61 complex, which is composed of SSH1, SBH1 and SSS1. Presumably three to four Sec61 heterotrimers assemble into an oligomeric ring with a central aeqeous pore. In cotranslational ER import, the pore diameter varies from 9-15 A in a ribosome-free resting state to 40-60 A in a functional state when associated with the ribosome. The Sec61 complex is part of a channel-forming translocon complex whose composition seem to change dependent upon different functional states. During post-translational ER import the Sec61 complex associates with the Sec62/63 complex to form the Sec complex. SBH1 interacts OST2, OST4 and WBP1 components of the OT complex. Ref.8
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the SEC61-beta family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	SRP-dependent cotranslational protein targeting to membrane, translocation Inferred from mutant phenotype. Source: SGD posttranslational protein targeting to membrane, translocation Inferred from direct assay. Source: SGD
Cellular component	Sec61 translocon complex Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ARF guanyl-nucleotide exchange factor activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction Ref.8. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
OST2	P46964	2	EBI-16410,EBI-12673
OST4	Q99380	2	EBI-16410,EBI-12689
WBP1	P33767	2	EBI-16410,EBI-12658

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 82	82	Protein transport protein SBH1		PRO_0000157261
Regions
	Topological domain	1 – 53	53	Cytoplasmic Potential
	Transmembrane	54 – 74	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P52870 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 23B0313EB37744FA	FASTA	82	8,711
	10 20 30 40 50 60 MSSPTPPGGQ RTLQKRKQGS SQKVAASAPK KNTNSNNSIL KIYSDEATGL RVDPLVVLFL 70 80 AVGFIFSVVA LHVISKVAGK LF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast protein translocation complex: isolation of two genes SEB1 and SEB2 encoding proteins homologous to the Sec61 beta subunit." Toikkanen J., Gatti E., Takei K., Saloheimo M., Olkkonen V.M., Soederlund H., de Camilli P., Keraenen S. Yeast 12:425-438(1996) [PubMed: 8740416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation." Hanein D., Matlack K.E., Jungnickel B., Plath K., Kalies K.-U., Miller K.R., Rapoport T.A., Akey C.W. Cell 87:721-732(1996) [PubMed: 8929540] [Abstract] Cited for: ELECTRON MICRSOCOPY OF THE SEC61 COMPLEX.
[5]	"The aqueous pore through the translocon has a diameter of 40-60 A during cotranslational protein translocation at the ER membrane." Hamman B.D., Chen J.C., Johnson E.E., Johnson A.E. Cell 89:535-544(1997) [PubMed: 9160745] [Abstract] Cited for: TRANSLOCON COMPLEX PORE.
[6]	"The translocon: a dynamic gateway at the ER membrane." Johnson A.E., van Waes M.A. Annu. Rev. Cell Dev. Biol. 15:799-842(1999) [PubMed: 10611978] [Abstract] Cited for: REVIEW ON THE TRANSLOCON COMPLEX.
[7]	"Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA." Prinz A., Behrens C., Rapoport T.A., Hartmann E., Kalies K.-U. EMBO J. 19:1900-1906(2000) [PubMed: 10775273] [Abstract] Cited for: ASSOCIATION OF THE SEC61 COMPLEX WITH RIBOSOMES.
[8]	"Subunits of the translocon interact with components of the oligosaccharyl transferase complex." Chavan M., Yan A., Lennarz W.J. J. Biol. Chem. 280:22917-22924(2005) [PubMed: 15831493] [Abstract] Cited for: INTERACTION WITH OST2; OST4 AND WBP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47789 mRNA. Translation: CAA87710.1. U18839 Genomic DNA. Translation: AAB64663.1. BK006939 Genomic DNA. Translation: DAA07748.1.
PIR	S68162.
RefSeq	NP_011011.1. NM_001180032.1.
3D structure databases
ProteinModelPortal	P52870.
SMR	P52870. Positions 45-80.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5681N.
IntAct	P52870. 13 interactions.
MINT	MINT-529808.
STRING	P52870.
Protein family/group databases
TCDB	3.A.5.8.1. general secretory pathway (Sec) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER087C-B; YER087C-B; YER087C-B.
GeneID	856821.
KEGG	sce:YER087C-B.
NMPDR	fig|4932.3.peg.2083.
Organism-specific databases
CYGD	YER087c-b.
SGD	S000002128. SBH1.
Phylogenomic databases
eggNOG	fuNOG12116.
GeneTree	EFGT00050000003437.
HOGENOM	HBG749385.
OrthoDB	EOG4SXRPB.
Gene expression databases
ArrayExpress	P52870.
Genevestigator	P52870.
GermOnline	YER087C-B. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005609. Sec61_beta. IPR016482. Sec61_beta_euk. [Graphical view]
KO	K09481.
PANTHER	PTHR13509. PTHR13509. 1 hit.
Pfam	PF03911. Sec61_beta. 1 hit. [Graphical view]
PIRSF	PIRSF006398. Sec61_beta_euk. 1 hit.
ProtoNet	Search...
Other
NextBio	983103.

Entry information

Entry name

SC6B1_YEAST

Accession

Primary (citable) accession number: P52870
Secondary accession number(s): D3DLZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program