ID   CWC23_YEAST             Reviewed;         283 AA.
AC   P52868; D6VU20; Q6B1S2;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2005, sequence version 2.
DT   24-JAN-2024, entry version 161.
DE   RecName: Full=Pre-mRNA-splicing factor CWC23;
DE   AltName: Full=Complexed with CEF1 protein 23;
GN   Name=CWC23; OrderedLocusNames=YGL128C; ORFNames=G2861;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8686378;
RX   DOI=10.1002/(sici)1097-0061(199602)12:2<145::aid-yea888>3.0.co;2-e;
RA   Rodriguez-Belmonte E., Rodriguez Torres A.M., Tizon B., Cadahia J.L.,
RA   Gonzalez-Siso I., Ramil E., Becerra M., Gonzalez-Dominguez M., Cerdan E.;
RT   "Sequence analysis of a 10 kb DNA fragment from yeast chromosome VII
RT   reveals a novel member of the DnaJ family.";
RL   Yeast 12:145-148(1996).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   FUNCTION.
RX   PubMed=10029993;
RX   DOI=10.1002/(sici)1097-0061(19990130)15:2<145::aid-yea346>3.0.co;2-j;
RA   Tizon B., Rodriguez-Torres A.M., Cerdan M.E.;
RT   "Disruption of six novel Saccharomyces cerevisiae genes reveals that
RT   YGL129c is necessary for growth in non-fermentable carbon sources, YGL128c
RT   for growth at low or high temperatures and YGL125w is implicated in the
RT   biosynthesis of methionine.";
RL   Yeast 15:145-154(1999).
RN   [6]
RP   IDENTIFICATION IN THE CWC COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA   Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT   "Proteomics analysis reveals stable multiprotein complexes in both fission
RT   and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT   splicing factors, and snRNAs.";
RL   Mol. Cell. Biol. 22:2011-2024(2002).
RN   [7]
RP   FUNCTION.
RX   PubMed=12847107; DOI=10.1074/jbc.m301080200;
RA   Taxis C., Hitt R., Park S.-H., Deak P.M., Kostova Z., Wolf D.H.;
RT   "Use of modular substrates demonstrates mechanistic diversity and reveals
RT   differences in chaperone requirement of ERAD.";
RL   J. Biol. Chem. 278:35903-35913(2003).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA   Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA   Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA   Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT   "Assigning function to yeast proteins by integration of technologies.";
RL   Mol. Cell 12:1353-1365(2003).
RN   [9]
RP   IDENTIFICATION OF PROBABLE INITIATION SITE.
RX   PubMed=12748633; DOI=10.1038/nature01644;
RA   Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT   "Sequencing and comparison of yeast species to identify genes and
RT   regulatory elements.";
RL   Nature 423:241-254(2003).
RN   [10]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [11]
RP   FUNCTION.
RX   PubMed=15170475; DOI=10.1038/sj.embor.7400172;
RA   Walsh P., Bursac D., Law Y.C., Cyr D., Lithgow T.;
RT   "The J-protein family: modulating protein assembly, disassembly and
RT   translocation.";
RL   EMBO Rep. 5:567-571(2004).
RN   [12]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. May be involved in endoplasmic
CC       reticulum-associated protein degradation (ERAD) and required for growth
CC       at low and high temperatures. {ECO:0000269|PubMed:10029993,
CC       ECO:0000269|PubMed:12847107, ECO:0000269|PubMed:15170475}.
CC   -!- SUBUNIT: Belongs to the CWC complex (or CEF1-associated complex), a
CC       spliceosome sub-complex reminiscent of a late-stage spliceosome
CC       composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2,
CC       CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24,
CC       CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9,
CC       PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3,
CC       SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
CC       {ECO:0000269|PubMed:11884590}.
CC   -!- INTERACTION:
CC       P52868; Q06411: SPP382; NbExp=6; IntAct=EBI-560, EBI-576;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC       {ECO:0000269|PubMed:14562095}.
CC   -!- SIMILARITY: Belongs to the DnaJ family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAT93027.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA60703.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA96837.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X87252; CAA60703.1; ALT_INIT; Genomic_DNA.
DR   EMBL; Z72650; CAA96837.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AY693008; AAT93027.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BK006941; DAA07981.1; -; Genomic_DNA.
DR   PIR; S64139; S64139.
DR   RefSeq; NP_011387.2; NM_001180993.1.
DR   PDB; 5Y88; EM; 3.70 A; T=1-283.
DR   PDBsum; 5Y88; -.
DR   AlphaFoldDB; P52868; -.
DR   EMDB; EMD-6817; -.
DR   SMR; P52868; -.
DR   BioGRID; 33123; 32.
DR   ComplexPortal; CPX-1651; PRP19-associated complex.
DR   DIP; DIP-6831N; -.
DR   IntAct; P52868; 17.
DR   MINT; P52868; -.
DR   STRING; 4932.YGL128C; -.
DR   MaxQB; P52868; -.
DR   PaxDb; 4932-YGL128C; -.
DR   PeptideAtlas; P52868; -.
DR   TopDownProteomics; P52868; -.
DR   EnsemblFungi; YGL128C_mRNA; YGL128C; YGL128C.
DR   GeneID; 852749; -.
DR   KEGG; sce:YGL128C; -.
DR   AGR; SGD:S000003096; -.
DR   SGD; S000003096; CWC23.
DR   VEuPathDB; FungiDB:YGL128C; -.
DR   eggNOG; KOG0716; Eukaryota.
DR   HOGENOM; CLU_063717_0_0_1; -.
DR   InParanoid; P52868; -.
DR   OMA; KHFKLPY; -.
DR   OrthoDB; 3538412at2759; -.
DR   BioCyc; YEAST:G3O-30624-MONOMER; -.
DR   BioGRID-ORCS; 852749; 3 hits in 10 CRISPR screens.
DR   PRO; PR:P52868; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P52868; Protein.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0005684; C:U2-type spliceosomal complex; IDA:SGD.
DR   GO; GO:0000390; P:spliceosomal complex disassembly; IGI:SGD.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; DnaJ domain; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR018253; DnaJ_domain_CS.
DR   InterPro; IPR036869; J_dom_sf.
DR   PANTHER; PTHR44313; DNAJ HOMOLOG SUBFAMILY C MEMBER 17; 1.
DR   PANTHER; PTHR44313:SF1; DNAJ HOMOLOG SUBFAMILY C MEMBER 17; 1.
DR   Pfam; PF00226; DnaJ; 1.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; Chaperone J-domain; 1.
DR   PROSITE; PS00636; DNAJ_1; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chaperone; Cytoplasm; mRNA processing; mRNA splicing;
KW   Nucleus; Reference proteome; Spliceosome.
FT   CHAIN           1..283
FT                   /note="Pre-mRNA-splicing factor CWC23"
FT                   /id="PRO_0000071129"
FT   DOMAIN          15..87
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
SQ   SEQUENCE   283 AA;  33237 MW;  AA1D0894328CAEA5 CRC64;
     MPGHELEDVI NQRLNLYDVL ELPTPLDVHT IYDDLPQIKR KYRTLALKYH PDKHPDNPSI
     IHKFHLLSTA TNILTNADVR PHYDRWLIEF LRKTNDIERN KLIQKLEESE SSTIPTTTPH
     PDLLQIQRHG ELLRKLKHFN LPYGDWKHLN TQDQENASQH PYYDCSTLRI VLDNFLQSNN
     KSNCLSHLRN QVFITLSANE IYDIYFSERN NYSKDDSIII YTVFDTPITA QHVFRNWSSG
     NLIPTVKDIS PLIPLHYYSD FNLETELNDD IARLVSNEPI LLD
//