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P52868

- CWC23_YEAST

UniProt

P52868 - CWC23_YEAST

Protein

Pre-mRNA-splicing factor CWC23

Gene

CWC23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Involved in pre-mRNA splicing. May be involved in endoplasmic reticulum-associated protein degradation (ERAD) and required for growth at low and high temperatures.3 Publications

    GO - Biological processi

    1. spliceosomal complex disassembly Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30624-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor CWC23
    Alternative name(s):
    Complexed with CEF1 protein 23
    Gene namesi
    Name:CWC23
    Ordered Locus Names:YGL128C
    ORF Names:G2861
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL128c.
    SGDiS000003096. CWC23.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. U2-type spliceosomal complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 283283Pre-mRNA-splicing factor CWC23PRO_0000071129Add
    BLAST

    Proteomic databases

    MaxQBiP52868.
    PaxDbiP52868.
    PeptideAtlasiP52868.

    Expressioni

    Gene expression databases

    GenevestigatoriP52868.

    Interactioni

    Subunit structurei

    Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

    Protein-protein interaction databases

    BioGridi33123. 33 interactions.
    DIPiDIP-6831N.
    IntActiP52868. 16 interactions.
    MINTiMINT-636455.
    STRINGi4932.YGL128C.

    Structurei

    3D structure databases

    ProteinModelPortaliP52868.
    SMRiP52868. Positions 38-85.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 8773JPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DnaJ family.Curated
    Contains 1 J domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2214.
    OMAiTNDIERN.
    OrthoDBiEOG7WT4DJ.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    InterProiIPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    [Graphical view]
    PfamiPF00226. DnaJ. 1 hit.
    [Graphical view]
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    PROSITEiPS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52868-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGHELEDVI NQRLNLYDVL ELPTPLDVHT IYDDLPQIKR KYRTLALKYH    50
    PDKHPDNPSI IHKFHLLSTA TNILTNADVR PHYDRWLIEF LRKTNDIERN 100
    KLIQKLEESE SSTIPTTTPH PDLLQIQRHG ELLRKLKHFN LPYGDWKHLN 150
    TQDQENASQH PYYDCSTLRI VLDNFLQSNN KSNCLSHLRN QVFITLSANE 200
    IYDIYFSERN NYSKDDSIII YTVFDTPITA QHVFRNWSSG NLIPTVKDIS 250
    PLIPLHYYSD FNLETELNDD IARLVSNEPI LLD 283
    Length:283
    Mass (Da):33,237
    Last modified:August 30, 2005 - v2
    Checksum:iAA1D0894328CAEA5
    GO

    Sequence cautioni

    The sequence AAT93027.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA60703.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA96837.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87252 Genomic DNA. Translation: CAA60703.1. Different initiation.
    Z72650 Genomic DNA. Translation: CAA96837.1. Different initiation.
    AY693008 Genomic DNA. Translation: AAT93027.1. Different initiation.
    BK006941 Genomic DNA. Translation: DAA07981.1.
    PIRiS64139.
    RefSeqiNP_011387.2. NM_001180993.1.

    Genome annotation databases

    EnsemblFungiiYGL128C; YGL128C; YGL128C.
    GeneIDi852749.
    KEGGisce:YGL128C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87252 Genomic DNA. Translation: CAA60703.1 . Different initiation.
    Z72650 Genomic DNA. Translation: CAA96837.1 . Different initiation.
    AY693008 Genomic DNA. Translation: AAT93027.1 . Different initiation.
    BK006941 Genomic DNA. Translation: DAA07981.1 .
    PIRi S64139.
    RefSeqi NP_011387.2. NM_001180993.1.

    3D structure databases

    ProteinModelPortali P52868.
    SMRi P52868. Positions 38-85.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33123. 33 interactions.
    DIPi DIP-6831N.
    IntActi P52868. 16 interactions.
    MINTi MINT-636455.
    STRINGi 4932.YGL128C.

    Proteomic databases

    MaxQBi P52868.
    PaxDbi P52868.
    PeptideAtlasi P52868.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL128C ; YGL128C ; YGL128C .
    GeneIDi 852749.
    KEGGi sce:YGL128C.

    Organism-specific databases

    CYGDi YGL128c.
    SGDi S000003096. CWC23.

    Phylogenomic databases

    eggNOGi COG2214.
    OMAi TNDIERN.
    OrthoDBi EOG7WT4DJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30624-MONOMER.

    Miscellaneous databases

    NextBioi 972177.

    Gene expression databases

    Genevestigatori P52868.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    InterProi IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    [Graphical view ]
    Pfami PF00226. DnaJ. 1 hit.
    [Graphical view ]
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    PROSITEi PS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 10 kb DNA fragment from yeast chromosome VII reveals a novel member of the DnaJ family."
      Rodriguez-Belmonte E., Rodriguez Torres A.M., Tizon B., Cadahia J.L., Gonzalez-Siso I., Ramil E., Becerra M., Gonzalez-Dominguez M., Cerdan E.
      Yeast 12:145-148(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Disruption of six novel Saccharomyces cerevisiae genes reveals that YGL129c is necessary for growth in non-fermentable carbon sources, YGL128c for growth at low or high temperatures and YGL125w is implicated in the biosynthesis of methionine."
      Tizon B., Rodriguez-Torres A.M., Cerdan M.E.
      Yeast 15:145-154(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Use of modular substrates demonstrates mechanistic diversity and reveals differences in chaperone requirement of ERAD."
      Taxis C., Hitt R., Park S.-H., Deak P.M., Kostova Z., Wolf D.H.
      J. Biol. Chem. 278:35903-35913(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "The J-protein family: modulating protein assembly, disassembly and translocation."
      Walsh P., Bursac D., Law Y.C., Cyr D., Lithgow T.
      EMBO Rep. 5:567-571(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCWC23_YEAST
    AccessioniPrimary (citable) accession number: P52868
    Secondary accession number(s): D6VU20, Q6B1S2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3