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P52868

- CWC23_YEAST

UniProt

P52868 - CWC23_YEAST

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Protein

Pre-mRNA-splicing factor CWC23

Gene

CWC23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. May be involved in endoplasmic reticulum-associated protein degradation (ERAD) and required for growth at low and high temperatures.3 Publications

GO - Biological processi

  1. spliceosomal complex disassembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30624-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC23
Alternative name(s):
Complexed with CEF1 protein 23
Gene namesi
Name:CWC23
Ordered Locus Names:YGL128C
ORF Names:G2861
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL128c.
SGDiS000003096. CWC23.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. U2-type spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Pre-mRNA-splicing factor CWC23PRO_0000071129Add
BLAST

Proteomic databases

MaxQBiP52868.
PaxDbiP52868.
PeptideAtlasiP52868.

Expressioni

Gene expression databases

GenevestigatoriP52868.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Protein-protein interaction databases

BioGridi33123. 34 interactions.
DIPiDIP-6831N.
IntActiP52868. 16 interactions.
MINTiMINT-636455.
STRINGi4932.YGL128C.

Structurei

3D structure databases

ProteinModelPortaliP52868.
SMRiP52868. Positions 38-85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 8773JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DnaJ family.Curated
Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2214.
InParanoidiP52868.
OMAiTNDIERN.
OrthoDBiEOG7WT4DJ.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52868-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGHELEDVI NQRLNLYDVL ELPTPLDVHT IYDDLPQIKR KYRTLALKYH
60 70 80 90 100
PDKHPDNPSI IHKFHLLSTA TNILTNADVR PHYDRWLIEF LRKTNDIERN
110 120 130 140 150
KLIQKLEESE SSTIPTTTPH PDLLQIQRHG ELLRKLKHFN LPYGDWKHLN
160 170 180 190 200
TQDQENASQH PYYDCSTLRI VLDNFLQSNN KSNCLSHLRN QVFITLSANE
210 220 230 240 250
IYDIYFSERN NYSKDDSIII YTVFDTPITA QHVFRNWSSG NLIPTVKDIS
260 270 280
PLIPLHYYSD FNLETELNDD IARLVSNEPI LLD
Length:283
Mass (Da):33,237
Last modified:August 30, 2005 - v2
Checksum:iAA1D0894328CAEA5
GO

Sequence cautioni

The sequence AAT93027.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA60703.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA96837.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87252 Genomic DNA. Translation: CAA60703.1. Different initiation.
Z72650 Genomic DNA. Translation: CAA96837.1. Different initiation.
AY693008 Genomic DNA. Translation: AAT93027.1. Different initiation.
BK006941 Genomic DNA. Translation: DAA07981.1.
PIRiS64139.
RefSeqiNP_011387.2. NM_001180993.1.

Genome annotation databases

EnsemblFungiiYGL128C; YGL128C; YGL128C.
GeneIDi852749.
KEGGisce:YGL128C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87252 Genomic DNA. Translation: CAA60703.1 . Different initiation.
Z72650 Genomic DNA. Translation: CAA96837.1 . Different initiation.
AY693008 Genomic DNA. Translation: AAT93027.1 . Different initiation.
BK006941 Genomic DNA. Translation: DAA07981.1 .
PIRi S64139.
RefSeqi NP_011387.2. NM_001180993.1.

3D structure databases

ProteinModelPortali P52868.
SMRi P52868. Positions 38-85.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33123. 34 interactions.
DIPi DIP-6831N.
IntActi P52868. 16 interactions.
MINTi MINT-636455.
STRINGi 4932.YGL128C.

Proteomic databases

MaxQBi P52868.
PaxDbi P52868.
PeptideAtlasi P52868.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL128C ; YGL128C ; YGL128C .
GeneIDi 852749.
KEGGi sce:YGL128C.

Organism-specific databases

CYGDi YGL128c.
SGDi S000003096. CWC23.

Phylogenomic databases

eggNOGi COG2214.
InParanoidi P52868.
OMAi TNDIERN.
OrthoDBi EOG7WT4DJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-30624-MONOMER.

Miscellaneous databases

NextBioi 972177.

Gene expression databases

Genevestigatori P52868.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
InterProi IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view ]
Pfami PF00226. DnaJ. 1 hit.
[Graphical view ]
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 10 kb DNA fragment from yeast chromosome VII reveals a novel member of the DnaJ family."
    Rodriguez-Belmonte E., Rodriguez Torres A.M., Tizon B., Cadahia J.L., Gonzalez-Siso I., Ramil E., Becerra M., Gonzalez-Dominguez M., Cerdan E.
    Yeast 12:145-148(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Disruption of six novel Saccharomyces cerevisiae genes reveals that YGL129c is necessary for growth in non-fermentable carbon sources, YGL128c for growth at low or high temperatures and YGL125w is implicated in the biosynthesis of methionine."
    Tizon B., Rodriguez-Torres A.M., Cerdan M.E.
    Yeast 15:145-154(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Use of modular substrates demonstrates mechanistic diversity and reveals differences in chaperone requirement of ERAD."
    Taxis C., Hitt R., Park S.-H., Deak P.M., Kostova Z., Wolf D.H.
    J. Biol. Chem. 278:35903-35913(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. "The J-protein family: modulating protein assembly, disassembly and translocation."
    Walsh P., Bursac D., Law Y.C., Cyr D., Lithgow T.
    EMBO Rep. 5:567-571(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCWC23_YEAST
AccessioniPrimary (citable) accession number: P52868
Secondary accession number(s): D6VU20, Q6B1S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: August 30, 2005
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3