P52868 (CWC23_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor CWC23

Alternative name(s):
Complexed with CEF1 protein 23

Gene names

Name:	CWC23
Ordered Locus Names:	YGL128C
ORF Names:	G2861

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing. May be involved in endoplasmic reticulum-associated protein degradation (ERAD) and required for growth at low and high temperatures. Ref.5 Ref.7 Ref.10
Subunit structure	Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.9.
Sequence similarities	Belongs to the DnaJ family. Contains 1 J domain.
Sequence caution	The sequence AAT93027.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA60703.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA96837.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus Spliceosome
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	spliceosomal complex disassembly Inferred from genetic interaction PubMed 19822657. Source: SGD
Cellular_component	U2-type spliceosomal complex Inferred from direct assay Ref.6. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Pre-mRNA-splicing factor CWC23		PRO_0000071129
Regions
	Domain	15 – 87	73	J

Sequences

	Sequence		Length	Mass (Da)
	P52868 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: AA1D0894328CAEA5	FASTA	283	33,237
	10 20 30 40 50 60 MPGHELEDVI NQRLNLYDVL ELPTPLDVHT IYDDLPQIKR KYRTLALKYH PDKHPDNPSI 70 80 90 100 110 120 IHKFHLLSTA TNILTNADVR PHYDRWLIEF LRKTNDIERN KLIQKLEESE SSTIPTTTPH 130 140 150 160 170 180 PDLLQIQRHG ELLRKLKHFN LPYGDWKHLN TQDQENASQH PYYDCSTLRI VLDNFLQSNN 190 200 210 220 230 240 KSNCLSHLRN QVFITLSANE IYDIYFSERN NYSKDDSIII YTVFDTPITA QHVFRNWSSG 250 260 270 280 NLIPTVKDIS PLIPLHYYSD FNLETELNDD IARLVSNEPI LLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 10 kb DNA fragment from yeast chromosome VII reveals a novel member of the DnaJ family." Rodriguez-Belmonte E., Rodriguez Torres A.M., Tizon B., Cadahia J.L., Gonzalez-Siso I., Ramil E., Becerra M., Gonzalez-Dominguez M., Cerdan E. Yeast 12:145-148(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Disruption of six novel Saccharomyces cerevisiae genes reveals that YGL129c is necessary for growth in non-fermentable carbon sources, YGL128c for growth at low or high temperatures and YGL125w is implicated in the biosynthesis of methionine." Tizon B., Rodriguez-Torres A.M., Cerdan M.E. Yeast 15:145-154(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[7]	"Use of modular substrates demonstrates mechanistic diversity and reveals differences in chaperone requirement of ERAD." Taxis C., Hitt R., Park S.-H., Deak P.M., Kostova Z., Wolf D.H. J. Biol. Chem. 278:35903-35913(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"The J-protein family: modulating protein assembly, disassembly and translocation." Walsh P., Bursac D., Law Y.C., Cyr D., Lithgow T. EMBO Rep. 5:567-571(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87252 Genomic DNA. Translation: CAA60703.1. Different initiation. Z72650 Genomic DNA. Translation: CAA96837.1. Different initiation. AY693008 Genomic DNA. Translation: AAT93027.1. Different initiation. BK006941 Genomic DNA. Translation: DAA07981.1.
PIR	S64139.
RefSeq	NP_011387.2. NM_001180993.1.
3D structure databases
ProteinModelPortal	P52868.
SMR	P52868. Positions 38-85.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6831N.
IntAct	P52868. 16 interactions.
MINT	MINT-636455.
STRING	4932.YGL128C.
Proteomic databases
PaxDb	P52868.
PeptideAtlas	P52868.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL128C; YGL128C; YGL128C.
GeneID	852749.
KEGG	sce:YGL128C.
Organism-specific databases
CYGD	YGL128c.
SGD	S000003096. CWC23.
Phylogenomic databases
eggNOG	COG2214.
OMA	HISRATT.
OrthoDB	EOG46T6B8.
Gene expression databases
Genevestigator	P52868.
GermOnline	YGL128C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
InterPro	IPR001623. DnaJ_domain. IPR018253. DnaJ_domain_CS. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972177.

Entry information

Entry name

CWC23_YEAST

Accession

Primary (citable) accession number: P52868
Secondary accession number(s): D6VU20, Q6B1S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	August 30, 2005
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program