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P52867

- PMT5_YEAST

UniProt

P52867 - PMT5_YEAST

Protein

Dolichyl-phosphate-mannose--protein mannosyltransferase 5

Gene

PMT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.By similarity

    Catalytic activityi

    Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

    GO - Molecular functioni

    1. dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. protein O-linked mannosylation Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YDL093W-MONOMER.
    BRENDAi2.4.1.109. 984.

    Protein family/group databases

    CAZyiGT39. Glycosyltransferase Family 39.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dolichyl-phosphate-mannose--protein mannosyltransferase 5 (EC:2.4.1.109)
    Gene namesi
    Name:PMT5
    Ordered Locus Names:YDL093W
    ORF Names:D2399
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL093w.
    SGDiS000002251. PMT5.

    Subcellular locationi

    GO - Cellular componenti

    1. dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt2p dimer complex Source: SGD
    2. dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt3p dimer complex Source: SGD
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 743743Dolichyl-phosphate-mannose--protein mannosyltransferase 5PRO_0000121495Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi380 – 3801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi386 – 3861N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP52867.
    PeptideAtlasiP52867.

    Expressioni

    Gene expression databases

    GenevestigatoriP52867.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PMT2P313822EBI-13591,EBI-13573

    Protein-protein interaction databases

    BioGridi31967. 25 interactions.
    DIPiDIP-5025N.
    IntActiP52867. 4 interactions.
    MINTiMINT-563573.
    STRINGi4932.YDL093W.

    Structurei

    3D structure databases

    ProteinModelPortaliP52867.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4646LumenalSequence AnalysisAdd
    BLAST
    Topological domaini68 – 12962CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini151 – 1588LumenalSequence Analysis
    Topological domaini180 – 1801CytoplasmicSequence Analysis
    Topological domaini202 – 23130LumenalSequence AnalysisAdd
    BLAST
    Topological domaini253 – 26412CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini286 – 583298LumenalSequence AnalysisAdd
    BLAST
    Topological domaini605 – 62319CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini645 – 6462LumenalSequence Analysis
    Topological domaini668 – 68316CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini705 – 74339LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei47 – 6721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei130 – 15021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei159 – 17921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei181 – 20121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei232 – 25221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei265 – 28521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei584 – 60421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei624 – 64421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei647 – 66721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei684 – 70421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini320 – 37455MIR 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini384 – 44461MIR 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini454 – 51057MIR 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 39 family.Curated
    Contains 3 MIR domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1928.
    GeneTreeiENSGT00740000116239.
    HOGENOMiHOG000157526.
    KOiK00728.
    OMAiMEFANDI.
    OrthoDBiEOG7BP89X.

    Family and domain databases

    InterProiIPR027005. GlyclTrfase_39_like.
    IPR003342. Glyco_trans_39.
    IPR016093. MIR_motif.
    IPR027004. PMT1/PTM5.
    [Graphical view]
    PANTHERiPTHR10050. PTHR10050. 1 hit.
    PTHR10050:SF28. PTHR10050:SF28. 1 hit.
    PfamiPF02815. MIR. 1 hit.
    PF02366. PMT. 1 hit.
    [Graphical view]
    SMARTiSM00472. MIR. 3 hits.
    [Graphical view]
    SUPFAMiSSF82109. SSF82109. 1 hit.
    PROSITEiPS50919. MIR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52867-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKEHLLKVD PIPDVTIKRG PLRSFLITKP CDNLSSLRTV TSSKEKLLVG    50
    CLLIFTAIVR LHNISLPNSV VFGENEVGTF VSQYVNNIFF TDVHPPLVAM 100
    LYATVSSVFG YKGLFNYGNI GTEYTANVPY VAMRFFSATL GIVSVLVLYL 150
    TLRVSGVKIA VAAICAVCFA IENSFVTLSR FTLIEGPFVF FMACAVYFFR 200
    RSELYLPNSC KANKSLLAAS IALGFAVSSK WAGLFTIAWA GIIVLWRVWF 250
    MIGDLSRPIG SSIKYMAFQF TCLLAIPAFI YFLIFSVHIK TLNVNGISSS 300
    FFPAEFRKTL KYNNVIKETV AEVAVGSAVS LNHVGTAGGY LHSHLHNYPA 350
    GSMQQQVTLY PHIDQNNKWI IELAEHPNEN VTSFQNLTDG TIIKLRQLKN 400
    GCRLHSHDHK PPVSQNADWQ KEVSCYGYEG FEGDINDDWI IEIDKKRSEP 450
    GPAQEHIRAI ETKFRLKHYL TGCYLFSHPE KLPEWGFGQQ EVTCAYFARE 500
    DLTSWYIEEN ENEISLPNPE KVSYKKMSFW QKFVAIHKFM FYLNNYMDTS 550
    HAYSSEPKTW PLMLRGIDFW NENGREVYFL GNAVLWWSVT AFICTFIIGV 600
    AVELLAWKLG VNILRDKHII NFHYQVFQYL LGFAAHYFPY FFVGQKLFLY 650
    DYLPAYYFGI LAFGHALDLI STYISNKRNN TGYIVVAIFM VVCFYFFSEH 700
    SPLIYATGWS SNLCKRSKWL GSWDFYCNSL LLSDSHYELN AES 743
    Length:743
    Mass (Da):84,776
    Last modified:October 1, 1996 - v1
    Checksum:iE75625C236AC6A68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92759 Genomic DNA. Translation: CAA63414.1.
    X95644 Genomic DNA. Translation: CAA64918.1.
    Z74142 Genomic DNA. Translation: CAA98661.1.
    BK006938 Genomic DNA. Translation: DAA11765.1.
    PIRiS67635.
    RefSeqiNP_010190.1. NM_001180152.1.

    Genome annotation databases

    EnsemblFungiiYDL093W; YDL093W; YDL093W.
    GeneIDi851464.
    KEGGisce:YDL093W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92759 Genomic DNA. Translation: CAA63414.1 .
    X95644 Genomic DNA. Translation: CAA64918.1 .
    Z74142 Genomic DNA. Translation: CAA98661.1 .
    BK006938 Genomic DNA. Translation: DAA11765.1 .
    PIRi S67635.
    RefSeqi NP_010190.1. NM_001180152.1.

    3D structure databases

    ProteinModelPortali P52867.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31967. 25 interactions.
    DIPi DIP-5025N.
    IntActi P52867. 4 interactions.
    MINTi MINT-563573.
    STRINGi 4932.YDL093W.

    Protein family/group databases

    CAZyi GT39. Glycosyltransferase Family 39.

    Proteomic databases

    MaxQBi P52867.
    PeptideAtlasi P52867.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL093W ; YDL093W ; YDL093W .
    GeneIDi 851464.
    KEGGi sce:YDL093W.

    Organism-specific databases

    CYGDi YDL093w.
    SGDi S000002251. PMT5.

    Phylogenomic databases

    eggNOGi COG1928.
    GeneTreei ENSGT00740000116239.
    HOGENOMi HOG000157526.
    KOi K00728.
    OMAi MEFANDI.
    OrthoDBi EOG7BP89X.

    Enzyme and pathway databases

    BioCyci YEAST:YDL093W-MONOMER.
    BRENDAi 2.4.1.109. 984.

    Miscellaneous databases

    NextBioi 968747.

    Gene expression databases

    Genevestigatori P52867.

    Family and domain databases

    InterProi IPR027005. GlyclTrfase_39_like.
    IPR003342. Glyco_trans_39.
    IPR016093. MIR_motif.
    IPR027004. PMT1/PTM5.
    [Graphical view ]
    PANTHERi PTHR10050. PTHR10050. 1 hit.
    PTHR10050:SF28. PTHR10050:SF28. 1 hit.
    Pfami PF02815. MIR. 1 hit.
    PF02366. PMT. 1 hit.
    [Graphical view ]
    SMARTi SM00472. MIR. 3 hits.
    [Graphical view ]
    SUPFAMi SSF82109. SSF82109. 1 hit.
    PROSITEi PS50919. MIR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Dommaschk U.
      Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames."
      Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A.
      Yeast 12:1377-1384(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiPMT5_YEAST
    AccessioniPrimary (citable) accession number: P52867
    Secondary accession number(s): D6VRQ5, Q92181
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3