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Protein

Diacylglycerol kinase theta

Gene

DGKQ

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity.By similarity1 Publication

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Enzyme regulationi

Inactivated by binding to RHOA. Not inhibited by phosphatidylserine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 108Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri121 – 168Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri183 – 234Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd BLAST52

GO - Molecular functioni

  • activating transcription factor binding Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: UniProtKB
  • kinase binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phospholipase binding Source: UniProtKB

GO - Biological processi

  • cAMP-mediated signaling Source: UniProtKB
  • diacylglycerol metabolic process Source: BHF-UCL
  • glycerolipid metabolic process Source: BHF-UCL
  • G-protein coupled receptor signaling pathway Source: UniProtKB
  • lipid phosphorylation Source: BHF-UCL
  • platelet activation Source: Reactome
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  • protein kinase C signaling Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • response to ATP Source: UniProtKB
  • thrombin-activated receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS07233-MONOMER.
ReactomeiR-HSA-114508. Effects of PIP2 hydrolysis.

Chemistry databases

SwissLipidsiSLP:000000740.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase theta (EC:2.7.1.107)
Short name:
DAG kinase theta
Alternative name(s):
Diglyceride kinase theta
Short name:
DGK-theta
Gene namesi
Name:DGKQ
Synonyms:DAGK4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:2856. DGKQ.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoskeleton Source: UniProtKB
  • cytosol Source: UniProtKB
  • nuclear speck Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi100C → G: Abolishes translocation to the plasma membrane. 1 Publication1
Mutagenesisi160C → G: Abolishes translocation to the plasma membrane. 1 Publication1
Mutagenesisi226C → G: Abolishes translocation to the plasma membrane. 1 Publication1
Mutagenesisi237G → R: Abolishes enzymatic activity; no effect on translocation to the plasma membrane. 2 Publications1
Mutagenesisi245P → A or L: Greatly reduces enzymatic activity. 1 Publication1
Mutagenesisi246P → L: Abolishes enzymatic activity. 1 Publication1

Organism-specific databases

DisGeNETi1609.
OpenTargetsiENSG00000145214.
PharmGKBiPA27317.

Polymorphism and mutation databases

BioMutaiDGKQ.
DMDMi257051005.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184671 – 942Diacylglycerol kinase thetaAdd BLAST942

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22PhosphoserineBy similarity1
Modified residuei26PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PRKCE and PRKCH in vitro.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP52824.
MaxQBiP52824.
PaxDbiP52824.
PeptideAtlasiP52824.
PRIDEiP52824.

PTM databases

iPTMnetiP52824.
PhosphoSitePlusiP52824.

Expressioni

Gene expression databases

BgeeiENSG00000145214.
CleanExiHS_DGKQ.
ExpressionAtlasiP52824. baseline and differential.
GenevisibleiP52824. HS.

Organism-specific databases

HPAiCAB033835.
HPA026797.

Interactioni

Subunit structurei

Interacts with RHOA (constitutively activated, GTP-bound); the interaction inhibits DGKQ. Interacts with PRKCE. Interacts with PRKCH. Interacts with PLCB1. Interacts with NR5A1; the interaction requires the LXXLL motif 1 and LXXLL motif 2 in DGKQ.5 Publications

GO - Molecular functioni

  • activating transcription factor binding Source: UniProtKB
  • kinase binding Source: UniProtKB
  • phospholipase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi107979. 9 interactors.
IntActiP52824. 1 interactor.
STRINGi9606.ENSP00000273814.

Structurei

3D structure databases

ProteinModelPortaliP52824.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini395 – 494Ras-associatingPROSITE-ProRule annotationAdd BLAST100
Domaini584 – 721DAGKcPROSITE-ProRule annotationAdd BLAST138

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi555 – 559LXXLL motif 15
Motifi574 – 578LXXLL motif 25

Domaini

The L-X-X-L-L repeats are implicated in binding to the nuclear receptor NR5A1.

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 3 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 108Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri121 – 168Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri183 – 234Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd BLAST52

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169. Eukaryota.
ENOG410XQVB. LUCA.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000007900.
HOVERGENiHBG094675.
InParanoidiP52824.
KOiK00901.
OMAiTKPTFCH.
OrthoDBiEOG091G02NN.
PhylomeDBiP52824.
TreeFamiTF312817.

Family and domain databases

CDDicd00029. C1. 2 hits.
InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
IPR000159. RA_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 3 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS50200. RA. 1 hit.
PS00479. ZF_DAG_PE_1. 3 hits.
PS50081. ZF_DAG_PE_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAEPGAR AWLGGGSPRP GSPACSPVLG SGGRARPGPG PGPGPERAGV
60 70 80 90 100
RAPGPAAAPG HSFRKVTLTK PTFCHLCSDF IWGLAGFLCD VCNFMSHEKC
110 120 130 140 150
LKHVRIPCTS VAPSLVRVPV AHCFGPRGLH KRKFCAVCRK VLEAPALHCE
160 170 180 190 200
VCELHLHPDC VPFACSDCRQ CHQDGHQDHD THHHHWREGN LPSGARCEVC
210 220 230 240 250
RKTCGSSDVL AGVRCEWCGV QAHSLCSAAL APECGFGRLR SLVLPPACVR
260 270 280 290 300
LLPGGFSKTQ SFRIVEAAEP GEGGDGADGS AAVGPGRETQ ATPESGKQTL
310 320 330 340 350
KIFDGDDAVR RSQFRLVTVS RLAGAEEVLE AALRAHHIPE DPGHLELCRL
360 370 380 390 400
PPSSQACDAW AGGKAGSAVI SEEGRSPGSG EATPEAWVIR ALPRAQEVLK
410 420 430 440 450
IYPGWLKVGV AYVSVRVTPK STARSVVLEV LPLLGRQAES PESFQLVEVA
460 470 480 490 500
MGCRHVQRTM LMDEQPLLDR LQDIRQMSVR QVSQTRFYVA ESRDVAPHVS
510 520 530 540 550
LFVGGLPPGL SPEEYSSLLH EAGATKATVV SVSHIYSSQG AVVLDVACFA
560 570 580 590 600
EAERLYMLLK DMAVRGRLLT ALVLPDLLHA KLPPDSCPLL VFVNPKSGGL
610 620 630 640 650
KGRDLLCSFR KLLNPHQVFD LTNGGPLPGL HLFSQVPCFR VLVCGGDGTV
660 670 680 690 700
GWVLGALEET RYRLACPEPS VAILPLGTGN DLGRVLRWGA GYSGEDPFSV
710 720 730 740 750
LLSVDEADAV LMDRWTILLD AHEAGSAEND TADAEPPKIV QMSNYCGIGI
760 770 780 790 800
DAELSLDFHQ AREEEPGKFT SRLHNKGVYV RVGLQKISHS RSLHKQIRLQ
810 820 830 840 850
VERQEVELPS IEGLIFINIP SWGSGADLWG SDSDTRFEKP RMDDGLLEVV
860 870 880 890 900
GVTGVVHMGQ VQGGLRSGIR IAQGSYFRVT LLKATPVQVD GEPWVQAPGH
910 920 930 940
MIISAAGPKV HMLRKAKQKP RRAGTTRDAR ADAAPAPESD PR
Length:942
Mass (Da):101,155
Last modified:September 1, 2009 - v2
Checksum:i836D4FCBC208A5B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45 – 46PE → RD in AAA98749 (PubMed:7607687).Curated2
Sequence conflicti50 – 56VRAPGPA → GVRARAR in AAA98749 (PubMed:7607687).Curated7
Sequence conflicti933A → R in AAA98749 (PubMed:7607687).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05847827P → L.1 PublicationCorresponds to variant rs17855876dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38707 mRNA. Translation: AAA98749.1.
AC019103 Genomic DNA. No translation available.
BC063801 mRNA. Translation: AAH63801.1.
CCDSiCCDS3342.1.
RefSeqiNP_001338.2. NM_001347.3.
UniGeneiHs.584858.

Genome annotation databases

EnsembliENST00000273814; ENSP00000273814; ENSG00000145214.
GeneIDi1609.
KEGGihsa:1609.
UCSCiuc003gbw.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38707 mRNA. Translation: AAA98749.1.
AC019103 Genomic DNA. No translation available.
BC063801 mRNA. Translation: AAH63801.1.
CCDSiCCDS3342.1.
RefSeqiNP_001338.2. NM_001347.3.
UniGeneiHs.584858.

3D structure databases

ProteinModelPortaliP52824.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107979. 9 interactors.
IntActiP52824. 1 interactor.
STRINGi9606.ENSP00000273814.

Chemistry databases

SwissLipidsiSLP:000000740.

PTM databases

iPTMnetiP52824.
PhosphoSitePlusiP52824.

Polymorphism and mutation databases

BioMutaiDGKQ.
DMDMi257051005.

Proteomic databases

EPDiP52824.
MaxQBiP52824.
PaxDbiP52824.
PeptideAtlasiP52824.
PRIDEiP52824.

Protocols and materials databases

DNASUi1609.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273814; ENSP00000273814; ENSG00000145214.
GeneIDi1609.
KEGGihsa:1609.
UCSCiuc003gbw.5. human.

Organism-specific databases

CTDi1609.
DisGeNETi1609.
GeneCardsiDGKQ.
HGNCiHGNC:2856. DGKQ.
HPAiCAB033835.
HPA026797.
MIMi601207. gene.
neXtProtiNX_P52824.
OpenTargetsiENSG00000145214.
PharmGKBiPA27317.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1169. Eukaryota.
ENOG410XQVB. LUCA.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000007900.
HOVERGENiHBG094675.
InParanoidiP52824.
KOiK00901.
OMAiTKPTFCH.
OrthoDBiEOG091G02NN.
PhylomeDBiP52824.
TreeFamiTF312817.

Enzyme and pathway databases

BioCyciZFISH:HS07233-MONOMER.
ReactomeiR-HSA-114508. Effects of PIP2 hydrolysis.

Miscellaneous databases

GeneWikiiDGKQ.
GenomeRNAii1609.
PROiP52824.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000145214.
CleanExiHS_DGKQ.
ExpressionAtlasiP52824. baseline and differential.
GenevisibleiP52824. HS.

Family and domain databases

CDDicd00029. C1. 2 hits.
InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
IPR000159. RA_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 3 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS50200. RA. 1 hit.
PS00479. ZF_DAG_PE_1. 3 hits.
PS50081. ZF_DAG_PE_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDGKQ_HUMAN
AccessioniPrimary (citable) accession number: P52824
Secondary accession number(s): Q6P3W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.