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P52824

- DGKQ_HUMAN

UniProt

P52824 - DGKQ_HUMAN

Protein

Diacylglycerol kinase theta

Gene

DGKQ

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor By similarity. May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity.By similarity1 Publication

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

    Enzyme regulationi

    Inactivated by binding to RHOA. Not inhibited by phosphatidylserine.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri60 – 10849Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri121 – 16848Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri183 – 23452Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. activating transcription factor binding Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. diacylglycerol kinase activity Source: UniProtKB
    4. kinase binding Source: UniProtKB
    5. metal ion binding Source: UniProtKB-KW
    6. NAD+ kinase activity Source: InterPro
    7. phospholipase binding Source: UniProtKB
    8. protein binding Source: UniProtKB

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. cAMP-mediated signaling Source: UniProtKB
    3. G-protein coupled receptor signaling pathway Source: UniProtKB
    4. platelet activation Source: Reactome
    5. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
    6. protein kinase C signaling Source: UniProtKB
    7. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    8. response to ATP Source: UniProtKB
    9. thrombin receptor signaling pathway Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_2202. Effects of PIP2 hydrolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase theta (EC:2.7.1.107)
    Short name:
    DAG kinase theta
    Alternative name(s):
    Diglyceride kinase theta
    Short name:
    DGK-theta
    Gene namesi
    Name:DGKQ
    Synonyms:DAGK4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:2856. DGKQ.

    Subcellular locationi

    Cytoplasm. Cell membrane. Cytoplasmcytoskeleton. Nucleus. Nucleus speckle
    Note: Translocates to the nucleus in response to thrombin stimulation By similarity. Translocates to the plasma membrane in response to steroid hormone receptor stimulation. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytoskeleton Source: UniProtKB
    3. cytosol Source: UniProtKB
    4. nuclear speck Source: UniProtKB
    5. nucleus Source: UniProtKB
    6. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi100 – 1001C → G: Abolishes translocation to the plasma membrane. 1 Publication
    Mutagenesisi160 – 1601C → G: Abolishes translocation to the plasma membrane. 1 Publication
    Mutagenesisi226 – 2261C → G: Abolishes translocation to the plasma membrane. 1 Publication
    Mutagenesisi237 – 2371G → R: Abolishes enzymatic activity; no effect on translocation to the plasma membrane. 2 Publications
    Mutagenesisi245 – 2451P → A or L: Greatly reduces enzymatic activity. 1 Publication
    Mutagenesisi246 – 2461P → L: Abolishes enzymatic activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA27317.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 942942Diacylglycerol kinase thetaPRO_0000218467Add
    BLAST

    Post-translational modificationi

    Phosphorylated by PRKCE and PRKCH in vitro.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP52824.
    PaxDbiP52824.
    PRIDEiP52824.

    PTM databases

    PhosphoSiteiP52824.

    Expressioni

    Gene expression databases

    ArrayExpressiP52824.
    BgeeiP52824.
    CleanExiHS_DGKQ.
    GenevestigatoriP52824.

    Organism-specific databases

    HPAiCAB033835.
    HPA026797.

    Interactioni

    Subunit structurei

    Interacts with RHOA (constitutively activated, GTP-bound); the interaction inhibits DGKQ. Interacts with PRKCE. Interacts with PRKCH. Interacts with PLCB1. Interacts with NR5A1; the interaction requires the LXXLL motif 1 and LXXLL motif 2 in DGKQ.5 Publications

    Protein-protein interaction databases

    BioGridi107979. 8 interactions.
    STRINGi9606.ENSP00000273814.

    Structurei

    3D structure databases

    ProteinModelPortaliP52824.
    SMRiP52824. Positions 54-108, 184-234.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini395 – 494100Ras-associatingPROSITE-ProRule annotationAdd
    BLAST
    Domaini584 – 721138DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi555 – 5595LXXLL motif 1
    Motifi574 – 5785LXXLL motif 2

    Domaini

    The L-X-X-L-L repeats are implicated in binding to the nuclear receptor NR5A1.

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 3 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 Ras-associating domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri60 – 10849Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri121 – 16848Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri183 – 23452Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    HOGENOMiHOG000007900.
    HOVERGENiHBG094675.
    InParanoidiP52824.
    KOiK00901.
    OMAiSDFIWGL.
    OrthoDBiEOG71VSS6.
    PhylomeDBiP52824.
    TreeFamiTF312817.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000159. Ras-assoc.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00788. RA. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 3 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00314. RA. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    PS50200. RA. 1 hit.
    PS00479. ZF_DAG_PE_1. 3 hits.
    PS50081. ZF_DAG_PE_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52824-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAAEPGAR AWLGGGSPRP GSPACSPVLG SGGRARPGPG PGPGPERAGV    50
    RAPGPAAAPG HSFRKVTLTK PTFCHLCSDF IWGLAGFLCD VCNFMSHEKC 100
    LKHVRIPCTS VAPSLVRVPV AHCFGPRGLH KRKFCAVCRK VLEAPALHCE 150
    VCELHLHPDC VPFACSDCRQ CHQDGHQDHD THHHHWREGN LPSGARCEVC 200
    RKTCGSSDVL AGVRCEWCGV QAHSLCSAAL APECGFGRLR SLVLPPACVR 250
    LLPGGFSKTQ SFRIVEAAEP GEGGDGADGS AAVGPGRETQ ATPESGKQTL 300
    KIFDGDDAVR RSQFRLVTVS RLAGAEEVLE AALRAHHIPE DPGHLELCRL 350
    PPSSQACDAW AGGKAGSAVI SEEGRSPGSG EATPEAWVIR ALPRAQEVLK 400
    IYPGWLKVGV AYVSVRVTPK STARSVVLEV LPLLGRQAES PESFQLVEVA 450
    MGCRHVQRTM LMDEQPLLDR LQDIRQMSVR QVSQTRFYVA ESRDVAPHVS 500
    LFVGGLPPGL SPEEYSSLLH EAGATKATVV SVSHIYSSQG AVVLDVACFA 550
    EAERLYMLLK DMAVRGRLLT ALVLPDLLHA KLPPDSCPLL VFVNPKSGGL 600
    KGRDLLCSFR KLLNPHQVFD LTNGGPLPGL HLFSQVPCFR VLVCGGDGTV 650
    GWVLGALEET RYRLACPEPS VAILPLGTGN DLGRVLRWGA GYSGEDPFSV 700
    LLSVDEADAV LMDRWTILLD AHEAGSAEND TADAEPPKIV QMSNYCGIGI 750
    DAELSLDFHQ AREEEPGKFT SRLHNKGVYV RVGLQKISHS RSLHKQIRLQ 800
    VERQEVELPS IEGLIFINIP SWGSGADLWG SDSDTRFEKP RMDDGLLEVV 850
    GVTGVVHMGQ VQGGLRSGIR IAQGSYFRVT LLKATPVQVD GEPWVQAPGH 900
    MIISAAGPKV HMLRKAKQKP RRAGTTRDAR ADAAPAPESD PR 942
    Length:942
    Mass (Da):101,155
    Last modified:September 1, 2009 - v2
    Checksum:i836D4FCBC208A5B6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 462PE → RD in AAA98749. (PubMed:7607687)Curated
    Sequence conflicti50 – 567VRAPGPA → GVRARAR in AAA98749. (PubMed:7607687)Curated
    Sequence conflicti933 – 9331A → R in AAA98749. (PubMed:7607687)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti27 – 271P → L.1 Publication
    Corresponds to variant rs17855876 [ dbSNP | Ensembl ].
    VAR_058478

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38707 mRNA. Translation: AAA98749.1.
    AC019103 Genomic DNA. No translation available.
    BC063801 mRNA. Translation: AAH63801.1.
    CCDSiCCDS3342.1.
    RefSeqiNP_001338.2. NM_001347.3.
    UniGeneiHs.584858.

    Genome annotation databases

    EnsembliENST00000273814; ENSP00000273814; ENSG00000145214.
    GeneIDi1609.
    KEGGihsa:1609.
    UCSCiuc003gbw.4. human.

    Polymorphism databases

    DMDMi257051005.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38707 mRNA. Translation: AAA98749.1 .
    AC019103 Genomic DNA. No translation available.
    BC063801 mRNA. Translation: AAH63801.1 .
    CCDSi CCDS3342.1.
    RefSeqi NP_001338.2. NM_001347.3.
    UniGenei Hs.584858.

    3D structure databases

    ProteinModelPortali P52824.
    SMRi P52824. Positions 54-108, 184-234.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107979. 8 interactions.
    STRINGi 9606.ENSP00000273814.

    PTM databases

    PhosphoSitei P52824.

    Polymorphism databases

    DMDMi 257051005.

    Proteomic databases

    MaxQBi P52824.
    PaxDbi P52824.
    PRIDEi P52824.

    Protocols and materials databases

    DNASUi 1609.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000273814 ; ENSP00000273814 ; ENSG00000145214 .
    GeneIDi 1609.
    KEGGi hsa:1609.
    UCSCi uc003gbw.4. human.

    Organism-specific databases

    CTDi 1609.
    GeneCardsi GC04M000942.
    HGNCi HGNC:2856. DGKQ.
    HPAi CAB033835.
    HPA026797.
    MIMi 601207. gene.
    neXtProti NX_P52824.
    PharmGKBi PA27317.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG47311.
    HOGENOMi HOG000007900.
    HOVERGENi HBG094675.
    InParanoidi P52824.
    KOi K00901.
    OMAi SDFIWGL.
    OrthoDBi EOG71VSS6.
    PhylomeDBi P52824.
    TreeFami TF312817.

    Enzyme and pathway databases

    Reactomei REACT_2202. Effects of PIP2 hydrolysis.

    Miscellaneous databases

    GeneWikii DGKQ.
    GenomeRNAii 1609.
    NextBioi 6614.
    PROi P52824.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52824.
    Bgeei P52824.
    CleanExi HS_DGKQ.
    Genevestigatori P52824.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000159. Ras-assoc.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00788. RA. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 3 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00314. RA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    PS50200. RA. 1 hit.
    PS00479. ZF_DAG_PE_1. 3 hits.
    PS50081. ZF_DAG_PE_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chromosomal localization of three mouse diacylglycerol kinase (DAGK) genes: genes sharing sequence homology to the Drosophila retinal degeneration A (rdgA) gene."
      Pilz A., Schaap D., Hunt D., Fitzgibbon J.
      Genomics 26:599-601(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-27.
      Tissue: Uterus.
    4. "Diacylglycerol kinase theta binds to and is negatively regulated by active RhoA."
      Houssa B., de Widt J., Kranenburg O., Moolenaar W.H., van Blitterswijk W.J.
      J. Biol. Chem. 274:6820-6822(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, INTERACTION WITH RHOA, SUBCELLULAR LOCATION.
    5. Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PLCB1.
    6. Cited for: INTERACTION WITH RHOA, MUTAGENESIS OF GLY-237; PRO-245 AND PRO-246.
    7. "Translocation of diacylglycerol kinase theta from cytosol to plasma membrane in response to activation of G protein-coupled receptors and protein kinase C."
      van Baal J., de Widt J., Divecha N., van Blitterswijk W.J.
      J. Biol. Chem. 280:9870-9878(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRKCE AND PRKCH, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-100; CYS-160; CYS-226 AND GLY-237.
    8. "Cyclic AMP-stimulated interaction between steroidogenic factor 1 and diacylglycerol kinase theta facilitates induction of CYP17."
      Li D., Urs A.N., Allegood J., Leon A., Merrill A.H. Jr., Sewer M.B.
      Mol. Cell. Biol. 27:6669-6685(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NR5A1.

    Entry informationi

    Entry nameiDGKQ_HUMAN
    AccessioniPrimary (citable) accession number: P52824
    Secondary accession number(s): Q6P3W4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3