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P52804 (EFNA5_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ephrin-A5
Alternative name(s):
EPH-related receptor tyrosine kinase ligand 7
Short name=LERK-7
Repulsive axon guidance signal protein
Gene names
Name:EFNA5
Synonyms:RAGS
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation By similarity. Induces growth cone collapse and repulsion of retinal ganglion cell axons.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Potential.

Tissue specificity

Expressed in a graded fashion across the tectum being more strongly expressed towards the posterior pole.

Sequence similarities

Belongs to the ephrin family.

Contains 1 ephrin RBD (ephrin receptor-binding) domain.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
   Cellular componentCell membrane
Membrane
   DomainSignal
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbrain development

Inferred from sequence or structural similarity. Source: AgBase

ephrin receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

negative chemotaxis

Inferred from sequence or structural similarity. Source: GOC

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of Rac GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of Rho GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of actin cytoskeleton organization

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cell-cell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of focal adhesion assembly

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of microtubule cytoskeleton organization

Inferred from sequence or structural similarity. Source: UniProtKB

retinal ganglion cell axon guidance

Inferred from sequence or structural similarity. Source: AgBase

   Cellular_componentanchored component of external side of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionchemorepellent activity

Inferred from sequence or structural similarity. Source: AgBase

ephrin receptor binding

Inferred from sequence or structural similarity. Source: AgBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 203183Ephrin-A5
PRO_0000008383
Propeptide204 – 22825Removed in mature form Potential
PRO_0000008384

Regions

Domain29 – 162134Ephrin RBD

Amino acid modifications

Lipidation2031GPI-anchor amidated asparagine Potential
Glycosylation371N-linked (GlcNAc...) Potential
Disulfide bond62 ↔ 102 By similarity
Disulfide bond90 ↔ 151 By similarity

Sequences

Sequence LengthMass (Da)Tools
P52804 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 56D8E4FBDECF18AD

FASTA22826,206
        10         20         30         40         50         60 
MPHVEMLLLA VAALWVCVRG QEPGRKAVAD RYAVYWNSTN PRFQQGDYHI DVCINDYLDV 

        70         80         90        100        110        120 
FCPHYEDSVP EDKTERYVLY MVNFDGYSSC DHISKGFKRW ECNRPHSPNG PLKFSEKFQL 

       130        140        150        160        170        180 
FTPFSLGFEF RPGREYFYIS SAIPDNGRRS CLKLKVFVRP ANSCMKTIGV HDRVFDVNDK 

       190        200        210        220 
VENSLEPADD TVRESAEPSR GENAAQTPRI PIRLLATLLF LLAMLLIL 

« Hide

References

[1]"In vitro guidance of retinal ganglion cell axons by RAGS, a 25 kDa tectal protein related to ligands for Eph receptor tyrosine kinases."
Drescher U., Kremoser C., Handwerker C., Loschinger J., Noda M., Bonhoeffer F.
Cell 82:359-370(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Posterior tectum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X90377 mRNA. Translation: CAA62027.1.
PIRA57084.
RefSeqNP_990515.1. NM_205184.2.
UniGeneGga.651.

3D structure databases

ProteinModelPortalP52804.
SMRP52804. Positions 28-165.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000000368.

Proteomic databases

PaxDbP52804.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396100.
KEGGgga:396100.

Organism-specific databases

CTD1946.

Phylogenomic databases

eggNOGNOG241965.
HOGENOMHOG000234373.
HOVERGENHBG051447.
InParanoidP52804.
KOK05462.
PhylomeDBP52804.

Family and domain databases

Gene3D2.60.40.420. 1 hit.
InterProIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERPTHR11304. PTHR11304. 1 hit.
PfamPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSPR01347. EPHRIN.
ProDomPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF49503. SSF49503. 1 hit.
PROSITEPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20816158.
PROP52804.

Entry information

Entry nameEFNA5_CHICK
AccessionPrimary (citable) accession number: P52804
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families