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Protein

Ephrin-A2

Gene

Efna2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. With the EPHA2 receptor may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.2 Publications

GO - Molecular functioni

  1. ephrin receptor binding Source: UniProtKB

GO - Biological processi

  1. axon guidance Source: GO_Central
  2. bone remodeling Source: UniProtKB
  3. ephrin receptor signaling pathway Source: UniProtKB
  4. olfactory bulb development Source: Ensembl
  5. osteoclast differentiation Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_243590. EPH-ephrin mediated repulsion of cells.
REACT_243939. EPHA-mediated growth cone collapse.
REACT_258158. EPH-Ephrin signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin-A2
Alternative name(s):
CEK7-ligand
Short name:
CEK7-L
ELF-1
EPH-related receptor tyrosine kinase ligand 6
Short name:
LERK-6
Gene namesi
Name:Efna2
Synonyms:Elf1, Epl6, Eplg6, Lerk6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:102707. Efna2.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. neuromuscular junction Source: Ensembl
  3. perikaryon Source: Ensembl
  4. plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 184164Ephrin-A2PRO_0000008363Add
BLAST
Propeptidei185 – 20925Removed in mature formSequence AnalysisPRO_0000008364Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi69 ↔ 110PROSITE-ProRule annotation
Disulfide bondi98 ↔ 159PROSITE-ProRule annotation
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis
Lipidationi184 – 1841GPI-anchor amidated asparagineSequence Analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP52801.
PRIDEiP52801.

PTM databases

PhosphoSiteiP52801.

Expressioni

Gene expression databases

BgeeiP52801.
CleanExiMM_EFNA2.
MM_ELF1.
GenevestigatoriP52801.

Interactioni

Subunit structurei

Binds to the receptor tyrosine kinases EPHA3, EPHA4 and EPHA5. Interacts with EPHA8; activates EPHA8.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003154.

Structurei

3D structure databases

ProteinModelPortaliP52801.
SMRiP52801. Positions 29-169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 170141Ephrin RBDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ephrin family.PROSITE-ProRule annotation
Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG252953.
GeneTreeiENSGT00390000015107.
HOGENOMiHOG000234373.
HOVERGENiHBG051447.
InParanoidiP52801.
KOiK05462.
OMAiCSSPGGC.
OrthoDBiEOG70W3FD.
PhylomeDBiP52801.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPAQRPLLP LLLLLLPLRA RNEDPARANA DRYAVYWNRS NPRFQVSAVG
60 70 80 90 100
DGGGYTVEVS INDYLDIYCP HYGAPLPPAE RMERYILYMV NGEGHASCDH
110 120 130 140 150
RQRGFKRWEC NRPAAPGGPL KFSEKFQLFT PFSLGFEFRP GHEYYYISAT
160 170 180 190 200
PPNLVDRPCL RLKVYVRPTN ETLYEAPEPI FTSNSSCSGL GGCHLFLTTV

PVLWSLLGS
Length:209
Mass (Da):23,586
Last modified:October 1, 1996 - v1
Checksum:iF1997545F25B9ABC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14941 mRNA. Translation: AAA53636.1.
U14752 mRNA. Translation: AAA68520.1.
BC048697 mRNA. Translation: AAH48697.1.
CCDSiCCDS24013.1.
PIRiA54984.
RefSeqiNP_031935.3. NM_007909.3.
UniGeneiMm.1478.

Genome annotation databases

EnsembliENSMUST00000003154; ENSMUSP00000003154; ENSMUSG00000003070.
GeneIDi13637.
KEGGimmu:13637.
UCSCiuc007gcg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14941 mRNA. Translation: AAA53636.1.
U14752 mRNA. Translation: AAA68520.1.
BC048697 mRNA. Translation: AAH48697.1.
CCDSiCCDS24013.1.
PIRiA54984.
RefSeqiNP_031935.3. NM_007909.3.
UniGeneiMm.1478.

3D structure databases

ProteinModelPortaliP52801.
SMRiP52801. Positions 29-169.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003154.

PTM databases

PhosphoSiteiP52801.

Proteomic databases

PaxDbiP52801.
PRIDEiP52801.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003154; ENSMUSP00000003154; ENSMUSG00000003070.
GeneIDi13637.
KEGGimmu:13637.
UCSCiuc007gcg.2. mouse.

Organism-specific databases

CTDi1943.
MGIiMGI:102707. Efna2.

Phylogenomic databases

eggNOGiNOG252953.
GeneTreeiENSGT00390000015107.
HOGENOMiHOG000234373.
HOVERGENiHBG051447.
InParanoidiP52801.
KOiK05462.
OMAiCSSPGGC.
OrthoDBiEOG70W3FD.
PhylomeDBiP52801.

Enzyme and pathway databases

ReactomeiREACT_243590. EPH-ephrin mediated repulsion of cells.
REACT_243939. EPHA-mediated growth cone collapse.
REACT_258158. EPH-Ephrin signaling.

Miscellaneous databases

ChiTaRSiEfna2. mouse.
NextBioi284324.
PROiP52801.
SOURCEiSearch...

Gene expression databases

BgeeiP52801.
CleanExiMM_EFNA2.
MM_ELF1.
GenevestigatoriP52801.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases."
    Cheng H.J., Flanagan J.G.
    Cell 79:157-168(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss Webster.
    Tissue: Brain.
  2. "cDNA cloning and characterization of a Cek7 receptor protein-tyrosine kinase ligand that is identical to the ligand (ELF-1) for the Mek-4 and Sek receptor protein-tyrosine kinases."
    Shao H., Lou L., Pandey A., Verderame M.F., Siever D.A., Dixit V.M.
    J. Biol. Chem. 270:3467-3470(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Limb.
  4. "The Eek receptor, a member of the Eph family of tyrosine protein kinases, can be activated by three different Eph family ligands."
    Park S., Sanchez M.P.
    Oncogene 14:533-542(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EPHA8 ACTIVATION, INTERACTION WITH EPHA8.
  5. "Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis."
    Irie N., Takada Y., Watanabe Y., Matsuzaki Y., Naruse C., Asano M., Iwakura Y., Suda T., Matsuo K.
    J. Biol. Chem. 284:14637-14644(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN BONE REMODELING.

Entry informationi

Entry nameiEFNA2_MOUSE
AccessioniPrimary (citable) accession number: P52801
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.