Reviewed, UniProtKB/Swiss-Prot P52800 (EFNB2_MOUSE)

Names and origin

Protein names

Recommended name:
    Ephrin-B2
Alternative name(s):
    EPH-related receptor tyrosine kinase ligand 5
      Short name=LERK-5
      Short name=HTK ligand
      Short name=HTK-L
    ELF-2

Gene names

Name:	Efnb2
Synonyms:	Elf2, Epl5, Eplg5, Htkl, Lerk5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to the receptor tyrosine kinases EPHB2 and EPHB4. May play a role in constraining the orientation of longitudinally projecting axons. Ref.5
Subunit structure	Interacts with PDZRN3 By similarity. Binds to the receptor tyrosine kinase EPHB4.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed on lateral floor plate cells, specifically on commissural axon segments that have passed through the floor plate. Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk.
Developmental stage	Expressed in the floor plate throughout the period of commissural axon pathfinding.
Post-translational modification	Inducible phosphorylation of tyrosine residues in the cytoplasmic domain By similarity.
Sequence similarities	Belongs to the ephrin family.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Membrane
Domain	Signal Transmembrane
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from direct assay. Source: UniProtKB cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW lymph vessel development Inferred from mutant phenotype. Source: MGI nervous system development Inferred from electronic annotation. Source: UniProtKB-KW organ morphogenesis Inferred from mutant phenotype. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: MGI
Molecular function	receptor binding Ref.8 Inferred from physical interaction. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Epha4	Q03137	1	EBI-1032676,EBI-1539152

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

28

Potential

Chain

29 – 336

308

Ephrin-B2

PRO_0000008393

Regions

Topological domain

29 – 232

204

Extracellular Potential

Transmembrane

233 – 253

21

Potential

Topological domain

254 – 336

83

Cytoplasmic Potential

Motif

334 – 336

3

PDZ-binding Potential

Amino acid modifications

Modified residue

307

1

Phosphotyrosine Ref.6

Modified residue

328

1

Phosphoserine By similarity

Modified residue

333

1

Phosphotyrosine By similarity

Glycosylation

39

1

N-linked (GlcNAc...)

Glycosylation

142

1

N-linked (GlcNAc...) Potential

Disulfide bond

65 ↔ 104

Disulfide bond

92 ↔ 156

Experimental info

Sequence conflict

3 – 4

2

Missing Ref.3

Sequence conflict

177

1

A → T in AAA99708. Ref.1

Secondary structure

1

.

.

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336

Helix Strand Turn

Details...

Turn

47 – 49

3

Beta strand

50 – 53

4

Beta strand

60 – 64

5

Beta strand

70 – 72

3

Beta strand

78 – 84

7

Helix

86 – 91

6

Beta strand

101 – 104

4

Beta strand

112 – 116

5

Beta strand

133 – 138

6

Helix

145 – 147

3

Helix

154 – 158

5

Beta strand

162 – 167

6

Sequences

	Sequence		Length	Mass (Da)
	P52800-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D08894996E399554	FASTA	336	37,202
	10 20 30 40 50 60 MAMARSRRDS VWKYCWGLLM VLCRTAISRS IVLEPIYWNS SNSKFLPGQG LVLYPQIGDK 70 80 90 100 110 120 LDIICPKVDS KTVGQYEYYK VYMVDKDQAD RCTIKKENTP LLNCARPDQD VKFTIKFQEF 130 140 150 160 170 180 SPNLWGLEFQ KNKDYYIIST SNGSLEGLDN QEGGVCQTRA MKILMKVGQD ASSAGSARNH 190 200 210 220 230 240 GPTRRPELEA GTNGRSSTTS PFVKPNPGSS TDGNSAGHSG NNLLGSEVAL FAGIASGCII 250 260 270 280 290 300 FIVIIITLVV LLLKYRRRHR KHSPQHTTTL SLSTLATPKR GGNNNGSEPS DVIIPLRTAD 310 320 330 SVFCPHYEKV SGDYGHPVYI VQEMPPQSPA NIYYKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of LERK-5: a ligand of the eph-related receptor tyrosine kinases." Cerretti D.P., Vanden Bos T., Nelson N., Kozlosky C.J., Reddy P., Maraskovsky E., Park L.S., Lyman S.D., Copeland N.G., Gilbert D.J., Jenkins N.A., Fletcher R.A. Mol. Immunol. 32:1197-1205(1995) [PubMed: 8559144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk." Bennett B.D., Zeigler F.C., Gu Q., Fendly B., Goddard A.D., Gillett N., Matthews W. Proc. Natl. Acad. Sci. U.S.A. 92:1866-1870(1995) [PubMed: 7534404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CB57BL/6J X SJL/J.
[3]	"ELF-2, a new member of the Eph ligand family, is segmentally expressed in mouse embryos in the region of the hindbrain and newly forming somites." Bergemann A.D., Cheng H.J., Brambilla R., Klein R., Flanagan J.G. Mol. Cell. Biol. 15:4921-4929(1995) [PubMed: 7651410] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[5]	"Complementary expression of transmembrane ephrins and their receptors in the mouse spinal cord: a possible role in constraining the orientation of longitudinally projecting axons." Imondi R., Wideman C., Kaprielian Z. Development 127:1397-1410(2000) [PubMed: 10704386] [Abstract] Cited for: FUNCTION.
[6]	"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M. Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-307, MASS SPECTROMETRY.
[7]	"Crystal structure of an ephrin ectodomain." Toth J., Cutforth T., Gelinas A.D., Bethoney K.A., Bard J., Harrison C.J. Dev. Cell 1:83-92(2001) [PubMed: 11703926] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 30-170.
[8]	"Crystal structure of an Eph receptor-ephrin complex." Himanen J.-P., Rajashankar K.R., Lackmann M., Cowan C.A., Henkemeyer M., Nikolov D.B. Nature 414:933-938(2001) [PubMed: 11780069] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 31-168 IN COMPLEX WITH EPHB2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U16819 mRNA. Translation: AAA99708.1.
L38847 mRNA. Translation: AAC42052.1.
U30244 mRNA. Translation: AAA82934.1.
BC057009 mRNA. Translation: AAH57009.1.

IPI

IPI00762720.

PIR

I49766.

RefSeq

NP_034241.2.

UniGene

Mm.209813

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IKO	X-ray	1.92	P	30-207	[»]
1KGY	X-ray	2.70	E/F/G/H	31-168	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:29208N.

IntAct

P52800. 2 interactions.

PTM databases

PhosphoSite

P52800.

Proteomic databases

PRIDE

P52800.

Genome annotation databases

Ensembl

ENSMUSG00000001300. Mus musculus. [Contig view]

GeneID

13642.

KEGG

mmu:13642.

Organism-specific databases

MGI

MGI:105097. Efnb2.

Phylogenomic databases

HOVERGEN

P52800.

Gene expression databases

ArrayExpress

P52800.

Bgee

P52800.

CleanEx

MM_EFNB2.
MM_ELF2.

GermOnline

ENSMUSG00000001300. Mus musculus.

Family and domain databases

InterPro

IPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]

Gene3D

G3DSA:2.60.40.420. Cupredoxin. 1 hit.

PANTHER

PTHR11304. Ephrin. 1 hit.

Pfam

PF00812. Ephrin. 1 hit.
[Graphical view]

PRINTS

PR01347. EPHRIN.

ProDom

PD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS01299. EPHRIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

284346.

SOURCE

Search...

Entry information

Entry name

EFNB2_MOUSE

Accession

Primary (citable) accession number: P52800

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 5, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)