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P52798

- EFNA4_HUMAN

UniProt

P52798 - EFNA4_HUMAN

Protein

Ephrin-A4

Gene

EFNA4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.

    GO - Molecular functioni

    1. ephrin receptor binding Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. transmembrane-ephrin receptor activity Source: ProtInc

    GO - Biological processi

    1. axon guidance Source: RefGenome
    2. bone remodeling Source: RefGenome
    3. cell-cell signaling Source: ProtInc
    4. ephrin receptor signaling pathway Source: RefGenome
    5. osteoclast differentiation Source: RefGenome

    Enzyme and pathway databases

    SignaLinkiP52798.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ephrin-A4
    Alternative name(s):
    EPH-related receptor tyrosine kinase ligand 4
    Short name:
    LERK-4
    Gene namesi
    Name:EFNA4
    Synonyms:EPLG4, LERK4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:3224. EFNA4.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: UniProtKB-SubCell
    3. integral component of plasma membrane Source: ProtInc
    4. plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27659.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 25251 PublicationAdd
    BLAST
    Chaini26 – 170145Ephrin-A4PRO_0000008373Add
    BLAST
    Propeptidei171 – 20131Removed in mature formSequence AnalysisPRO_0000008374Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi58 ↔ 99PROSITE-ProRule annotation
    Disulfide bondi86 ↔ 144PROSITE-ProRule annotation
    Lipidationi170 – 1701GPI-anchor amidated serineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiP52798.
    PRIDEiP52798.

    PTM databases

    PhosphoSiteiP52798.

    Expressioni

    Tissue specificityi

    Expressed in the adult spleen, lymph node, prostate, ovary, small intestine, and colon, and in fetal heart, lung, liver and kidney. Also detected in hematopoietic cell lines.

    Gene expression databases

    ArrayExpressiP52798.
    BgeeiP52798.
    CleanExiHS_EFNA4.
    GenevestigatoriP52798.

    Organism-specific databases

    HPAiCAB021350.

    Interactioni

    Protein-protein interaction databases

    BioGridi108265. 1 interaction.
    DIPiDIP-48295N.
    IntActiP52798. 1 interaction.
    STRINGi9606.ENSP00000403005.

    Structurei

    3D structure databases

    ProteinModelPortaliP52798.
    SMRiP52798. Positions 24-151.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 155130Ephrin RBDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ephrin family.PROSITE-ProRule annotation
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG266803.
    HOGENOMiHOG000234373.
    HOVERGENiHBG051447.
    InParanoidiP52798.
    KOiK05462.
    OMAiCCKESKS.
    OrthoDBiEOG70W3FD.
    PhylomeDBiP52798.

    Family and domain databases

    Gene3Di2.60.40.420. 1 hit.
    InterProiIPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view]
    PANTHERiPTHR11304. PTHR11304. 1 hit.
    PfamiPF00812. Ephrin. 1 hit.
    [Graphical view]
    PRINTSiPR01347. EPHRIN.
    ProDomiPD002533. Ephrin. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF49503. SSF49503. 1 hit.
    PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P52798-1) [UniParc]FASTAAdd to Basket

    Also known as: GPI-anchored

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRLLPLLRTV LWAAFLGSPL RGGSSLRHVV YWNSSNPRLL RGDAVVELGL    50
    NDYLDIVCPH YEGPGPPEGP ETFALYMVDW PGYESCQAEG PRAYKRWVCS 100
    LPFGHVQFSE KIQRFTPFSL GFEFLPGETY YYISVPTPES SGQCLRLQVS 150
    VCCKERKSES AHPVGSPGES GTSGWRGGDT PSPLCLLLLL LLLILRLLRI 200
    L 201
    Length:201
    Mass (Da):22,386
    Last modified:October 1, 1996 - v1
    Checksum:iABE8D5443A9AF28D
    GO
    Isoform 2 (identifier: P52798-2) [UniParc]FASTAAdd to Basket

    Also known as: Secreted

    The sequence of this isoform differs from the canonical sequence as follows:
         157-201: KSESAHPVGS...LLILRLLRIL → NLPSHPKEPE...VPIQTDKMEH

    Show »
    Length:193
    Mass (Da):21,699
    Checksum:iD0817324CC728EF5
    GO
    Isoform 3 (identifier: P52798-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         157-201: KSESAHPVGS...LLILRLLRIL → RARVLPRSPG...LAGACPLITG

    Note: Gene prediction based on EST data.

    Show »
    Length:207
    Mass (Da):22,584
    Checksum:i67767F6020B4530B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei157 – 20145KSESA…LLRIL → NLPSHPKEPESSQDPLEEEG SLLPALGVPIQTDKMEH in isoform 2. 1 PublicationVSP_001448Add
    BLAST
    Alternative sequencei157 – 20145KSESA…LLRIL → RARVLPRSPGGGGIPAACTG GANSDRQDGALMGEIRGSEV TLAGACPLITG in isoform 3. CuratedVSP_046707Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14188 mRNA. Translation: AAC50079.1.
    AJ006352 mRNA. Translation: CAA06992.1.
    AJ006353 mRNA. Translation: CAA06993.1.
    CR533569 mRNA. Translation: CAG38600.1.
    AL691442 Genomic DNA. No translation available.
    CH471121 Genomic DNA. Translation: EAW53138.1.
    CH471121 Genomic DNA. Translation: EAW53139.1.
    BC107483 mRNA. Translation: AAI07484.1.
    CCDSiCCDS1089.1. [P52798-1]
    CCDS41407.1. [P52798-2]
    CCDS44237.1. [P52798-3]
    PIRiI38850.
    RefSeqiNP_005218.1. NM_005227.2. [P52798-1]
    NP_872631.1. NM_182689.1. [P52798-3]
    NP_872632.2. NM_182690.2. [P52798-2]
    UniGeneiHs.449913.
    Hs.639422.

    Genome annotation databases

    EnsembliENST00000359751; ENSP00000352789; ENSG00000243364. [P52798-2]
    ENST00000368409; ENSP00000357394; ENSG00000243364. [P52798-1]
    ENST00000427683; ENSP00000414378; ENSG00000243364. [P52798-3]
    GeneIDi1945.
    KEGGihsa:1945.
    UCSCiuc001fhc.3. human.
    uc001fhd.3. human. [P52798-1]
    uc001fhe.3. human. [P52798-2]

    Polymorphism databases

    DMDMi1706672.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14188 mRNA. Translation: AAC50079.1 .
    AJ006352 mRNA. Translation: CAA06992.1 .
    AJ006353 mRNA. Translation: CAA06993.1 .
    CR533569 mRNA. Translation: CAG38600.1 .
    AL691442 Genomic DNA. No translation available.
    CH471121 Genomic DNA. Translation: EAW53138.1 .
    CH471121 Genomic DNA. Translation: EAW53139.1 .
    BC107483 mRNA. Translation: AAI07484.1 .
    CCDSi CCDS1089.1. [P52798-1 ]
    CCDS41407.1. [P52798-2 ]
    CCDS44237.1. [P52798-3 ]
    PIRi I38850.
    RefSeqi NP_005218.1. NM_005227.2. [P52798-1 ]
    NP_872631.1. NM_182689.1. [P52798-3 ]
    NP_872632.2. NM_182690.2. [P52798-2 ]
    UniGenei Hs.449913.
    Hs.639422.

    3D structure databases

    ProteinModelPortali P52798.
    SMRi P52798. Positions 24-151.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108265. 1 interaction.
    DIPi DIP-48295N.
    IntActi P52798. 1 interaction.
    STRINGi 9606.ENSP00000403005.

    PTM databases

    PhosphoSitei P52798.

    Polymorphism databases

    DMDMi 1706672.

    Proteomic databases

    PaxDbi P52798.
    PRIDEi P52798.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000359751 ; ENSP00000352789 ; ENSG00000243364 . [P52798-2 ]
    ENST00000368409 ; ENSP00000357394 ; ENSG00000243364 . [P52798-1 ]
    ENST00000427683 ; ENSP00000414378 ; ENSG00000243364 . [P52798-3 ]
    GeneIDi 1945.
    KEGGi hsa:1945.
    UCSCi uc001fhc.3. human.
    uc001fhd.3. human. [P52798-1 ]
    uc001fhe.3. human. [P52798-2 ]

    Organism-specific databases

    CTDi 1945.
    GeneCardsi GC01P155036.
    HGNCi HGNC:3224. EFNA4.
    HPAi CAB021350.
    MIMi 601380. gene.
    neXtProti NX_P52798.
    PharmGKBi PA27659.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG266803.
    HOGENOMi HOG000234373.
    HOVERGENi HBG051447.
    InParanoidi P52798.
    KOi K05462.
    OMAi CCKESKS.
    OrthoDBi EOG70W3FD.
    PhylomeDBi P52798.

    Enzyme and pathway databases

    SignaLinki P52798.

    Miscellaneous databases

    GeneWikii EFNA4.
    GenomeRNAii 1945.
    NextBioi 7879.
    PROi P52798.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52798.
    Bgeei P52798.
    CleanExi HS_EFNA4.
    Genevestigatori P52798.

    Family and domain databases

    Gene3Di 2.60.40.420. 1 hit.
    InterProi IPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view ]
    PANTHERi PTHR11304. PTHR11304. 1 hit.
    Pfami PF00812. Ephrin. 1 hit.
    [Graphical view ]
    PRINTSi PR01347. EPHRIN.
    ProDomi PD002533. Ephrin. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF49503. SSF49503. 1 hit.
    PROSITEi PS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ligands for the receptor tyrosine kinases hek and elk: isolation of cDNAs encoding a family of proteins."
      Kozlosky C.J., Maraskovsky E., McGrew J.T., Vanden Bos T., Teepe M., Lyman S.D., Srinivasan S., Fletcher F.A., Gayle R.B. III, Cerretti D.P., Beckmann M.P.
      Oncogene 10:299-306(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "A splice variant of human ephrin-A4 encodes a soluble molecule that is secreted by activated human B lymphocytes."
      Aasheim H.-C., Munthe E., Funderud S., Smeland E.B., Beiske K., Logtenberg T.
      Blood 95:221-230(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: B-cell.
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    7. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z., Henzel W.J.
      Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 26-40.

    Entry informationi

    Entry nameiEFNA4_HUMAN
    AccessioniPrimary (citable) accession number: P52798
    Secondary accession number(s): C9JHJ8
    , G3XAK2, O95457, Q5SR71, Q6FI57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3