P52797 (EFNA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ephrin-A3

Alternative name(s):
EFL-2
EHK1 ligand
Short name=EHK1-L
EPH-related receptor tyrosine kinase ligand 3
Short name=LERK-3

Gene names

Name:	EFNA3
Synonyms:	EFL2, EPLG3, LERK3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling By similarity.
Subunit structure	Interacts with EPHA8; activates EPHA8.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Expressed in brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes.
Sequence similarities	Belongs to the ephrin family. Contains 1 ephrin RBD (ephrin receptor-binding) domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement PubMed 8660976. Source: ProtInc
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement PubMed 8660976. Source: ProtInc
Molecular_function	transmembrane-ephrin receptor activity Traceable author statement PubMed 8660976. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 214	192	Ephrin-A3		PRO_0000008369
	Propeptide	215 – 238	24	Removed in mature form Potential		PRO_0000008370
Regions
	Domain	30 – 169	140	Ephrin RBD
Amino acid modifications
	Lipidation	214	1	GPI-anchor amidated glycine Potential
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	67	1	N-linked (GlcNAc...) Potential
	Glycosylation	100	1	N-linked (GlcNAc...) Potential
	Disulfide bond	63 ↔ 110		By similarity
	Disulfide bond	99 ↔ 158		By similarity
Natural variations
	Natural variant	190	1	V → M. Corresponds to variant rs17723260 [ dbSNP | Ensembl ].		VAR_048937
Experimental info
	Sequence conflict	71 – 74	4	Missing in AAA52368. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P52797 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8EFD6AE8FE33FDDA	FASTA	238	26,350
	10 20 30 40 50 60 MAAAPLLLLL LLVPVPLLPL LAQGPGGALG NRHAVYWNSS NQHLRREGYT VQVNVNDYLD 70 80 90 100 110 120 IYCPHYNSSG VGPGAGPGPG GGAEQYVLYM VSRNGYRTCN ASQGFKRWEC NRPHAPHSPI 130 140 150 160 170 180 KFSEKFQRYS AFSLGYEFHA GHEYYYISTP THNLHWKCLR MKVFVCCAST SHSGEKPVPT 190 200 210 220 230 LPQFTMGPNV KINVLEDFEG ENPQVPKLEK SISGTSPKRE HLPLAVGIAF FLMTFLAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ligands for the receptor tyrosine kinases hek and elk: isolation of cDNAs encoding a family of proteins." Kozlosky C.J., Maraskovsky E., McGrew J.T., Vanden Bos T., Teepe M., Lyman S.D., Srinivasan S., Fletcher F.A., Gayle R.B. III, Cerretti D.P., Beckmann M.P. Oncogene 10:299-306(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity." Davis S., Gale N.W., Aldrich T.H., Maisonpierre P.C., Lhotak V., Pawson T., Goldfarb M., Yancopoulos G.D. Science 266:816-819(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Duodenum.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14187 mRNA. Translation: AAC50078.1. L37360 mRNA. Translation: AAA52368.1. AL691442 Genomic DNA. Translation: CAI15318.1. CH471121 Genomic DNA. Translation: EAW53137.1. BC017722 mRNA. Translation: AAH17722.1. BC110406 mRNA. Translation: AAI10407.1.
IPI	IPI00005123.
PIR	I38849.
RefSeq	NP_004943.1. NM_004952.4.
UniGene	Hs.516656.
3D structure databases
ProteinModelPortal	P52797.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52797. 1 interaction.
MINT	MINT-1378559.
STRING	9606.ENSP00000357393.
Polymorphism databases
DMDM	1706671.
Proteomic databases
PRIDE	P52797.
Protocols and materials databases
DNASU	1944.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000368408; ENSP00000357393; ENSG00000143590.
GeneID	1944.
KEGG	hsa:1944.
UCSC	uc001fhf.3. human.
Organism-specific databases
CTD	1944.
GeneCards	GC01P155051.
HGNC	HGNC:3223. EFNA3.
HPA	CAB010494.
MIM	601381. gene.
neXtProt	NX_P52797.
PharmGKB	PA27658.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG295575.
HOGENOM	HOG000234373.
HOVERGEN	HBG051447.
InParanoid	P52797.
KO	K05462.
OrthoDB	EOG4GB77B.
PhylomeDB	P52797.
Enzyme and pathway databases
Pathway_Interaction_DB	epha_fwdpathway. EPHA forward signaling.
SignaLink	P52797.
Gene expression databases
ArrayExpress	P52797.
Bgee	P52797.
CleanEx	HS_EFNA3.
Genevestigator	P52797.
GermOnline	ENSG00000143590. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.420. 1 hit.
InterPro	IPR008972. Cupredoxin. IPR001799. Ephrin. IPR019765. Ephrin_CS. [Graphical view]
PANTHER	PTHR11304. PTHR11304. 1 hit.
Pfam	PF00812. Ephrin. 1 hit. [Graphical view]
PRINTS	PR01347. EPHRIN.
ProDom	PD002533. Ephrin. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF49503. Cupredoxin. 1 hit.
PROSITE	PS01299. EPHRIN_RBD_1. 1 hit. PS51551. EPHRIN_RBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1944.
NextBio	7875.
SOURCE	Search...

Entry information

Entry name

EFNA3_HUMAN

Accession

Primary (citable) accession number: P52797
Secondary accession number(s): D3DV85, Q0VGC9, Q5SR70

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.