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P52795

- EFNB1_MOUSE

UniProt

P52795 - EFNB1_MOUSE

Protein

Ephrin-B1

Gene

Efnb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to the receptor tyrosine kinases EPHB3 (preferred), EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.1 Publication

    GO - Molecular functioni

    1. ephrin receptor binding Source: MGI
    2. protein binding Source: IntAct

    GO - Biological processi

    1. axon guidance Source: MGI
    2. embryonic pattern specification Source: MGI
    3. ephrin receptor signaling pathway Source: RefGenome
    4. neural crest cell migration Source: MGI
    5. positive regulation of T cell proliferation Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ephrin-B1
    Alternative name(s):
    CEK5 receptor ligand
    Short name:
    CEK5-L
    ELK ligand
    Short name:
    ELK-L
    EPH-related receptor tyrosine kinase ligand 2
    Short name:
    LERK-2
    Stimulated by retinoic acid gene 1 protein
    Gene namesi
    Name:Efnb1
    Synonyms:Epl2, Eplg2, Lerk2, Stra1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:102708. Efnb1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane raft Source: MGI
    4. nucleus Source: MGI
    5. plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 345321Ephrin-B1PRO_0000008388Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi64 ↔ 101PROSITE-ProRule annotation
    Disulfide bondi89 ↔ 153PROSITE-ProRule annotation
    Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence Analysis
    Modified residuei286 – 2861PhosphoserineBy similarity

    Post-translational modificationi

    Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP52795.
    PaxDbiP52795.
    PRIDEiP52795.

    PTM databases

    PhosphoSiteiP52795.

    Expressioni

    Tissue specificityi

    Expressed on lateral floor plate cells, specifically on commissural axon segments that have passed through the floor plate. Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disc.

    Developmental stagei

    Expressed in the floor plate throughout the period of commissural axon pathfinding.

    Gene expression databases

    ArrayExpressiP52795.
    BgeeiP52795.
    CleanExiMM_EFNB1.
    GenevestigatoriP52795.

    Interactioni

    Subunit structurei

    Interacts with GRIP1 and GRIP2.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Psen1P497692EBI-8107507,EBI-990067
    SdcbpO089923EBI-8107507,EBI-538265

    Protein-protein interaction databases

    BioGridi199394. 1 interaction.
    DIPiDIP-29206N.
    IntActiP52795. 5 interactions.
    MINTiMINT-1793539.
    STRINGi10090.ENSMUSP00000050716.

    Structurei

    3D structure databases

    ProteinModelPortaliP52795.
    SMRiP52795. Positions 32-167.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 236212ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini258 – 34588CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 164135Ephrin RBDPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi343 – 3453PDZ-bindingSequence Analysis

    Sequence similaritiesi

    Belongs to the ephrin family.PROSITE-ProRule annotation
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG262190.
    HOGENOMiHOG000220931.
    HOVERGENiHBG051448.
    InParanoidiP52795.
    KOiK05463.
    OMAiPDSFFNS.
    OrthoDBiEOG7288S5.
    PhylomeDBiP52795.

    Family and domain databases

    Gene3Di2.60.40.420. 1 hit.
    InterProiIPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view]
    PANTHERiPTHR11304. PTHR11304. 1 hit.
    PfamiPF00812. Ephrin. 1 hit.
    [Graphical view]
    PRINTSiPR01347. EPHRIN.
    ProDomiPD002533. Ephrin. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF49503. SSF49503. 1 hit.
    PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52795-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARPGQRWLS KWLVAMVVLT LCRLATPLAK NLEPVSWSSL NPKFLSGKGL    50
    VIYPKIGDKL DIICPRAEAG RPYEYYKLYL VRPEQAAACS TVLDPNVLVT 100
    CNKPHQEIRF TIKFQEFSPN YMGLEFKKYH DYYITSTSNG SLEGLENREG 150
    GVCRTRTMKI VMKVGQDPNA VTPEQLTTSR PSKESDNTVK TATQAPGRGS 200
    QGDSDGKHET VNQEEKSGPG AGGGGSGDSD SFFNSKVALF AAVGAGCVIF 250
    LLIIIFLTVL LLKLRKRHRK HTQQRAAALS LSTLASPKGG SGTAGTEPSD 300
    IIIPLRTTEN NYCPHYEKVS GDYGHPVYIV QEMPPQSPAN IYYKV 345
    Length:345
    Mass (Da):37,859
    Last modified:October 1, 1996 - v1
    Checksum:i8C96FD3DC5CBC405
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti90 – 901S → T in AAA53231. (PubMed:7649373)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07602
    , U07598, U07599, U07600 Genomic DNA. Translation: AAC53247.1.
    Z48781 mRNA. Translation: CAA88695.1.
    U12983 mRNA. Translation: AAA53231.1.
    BC006797 mRNA. Translation: AAH06797.1.
    BC021656 mRNA. Translation: AAH21656.1.
    CCDSiCCDS30298.1.
    PIRiI48780.
    RefSeqiNP_034240.1. NM_010110.4.
    UniGeneiMm.3374.

    Genome annotation databases

    EnsembliENSMUST00000052839; ENSMUSP00000050716; ENSMUSG00000031217.
    GeneIDi13641.
    KEGGimmu:13641.
    UCSCiuc009tvi.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07602
    , U07598 , U07599 , U07600 Genomic DNA. Translation: AAC53247.1 .
    Z48781 mRNA. Translation: CAA88695.1 .
    U12983 mRNA. Translation: AAA53231.1 .
    BC006797 mRNA. Translation: AAH06797.1 .
    BC021656 mRNA. Translation: AAH21656.1 .
    CCDSi CCDS30298.1.
    PIRi I48780.
    RefSeqi NP_034240.1. NM_010110.4.
    UniGenei Mm.3374.

    3D structure databases

    ProteinModelPortali P52795.
    SMRi P52795. Positions 32-167.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199394. 1 interaction.
    DIPi DIP-29206N.
    IntActi P52795. 5 interactions.
    MINTi MINT-1793539.
    STRINGi 10090.ENSMUSP00000050716.

    PTM databases

    PhosphoSitei P52795.

    Proteomic databases

    MaxQBi P52795.
    PaxDbi P52795.
    PRIDEi P52795.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000052839 ; ENSMUSP00000050716 ; ENSMUSG00000031217 .
    GeneIDi 13641.
    KEGGi mmu:13641.
    UCSCi uc009tvi.1. mouse.

    Organism-specific databases

    CTDi 1947.
    MGIi MGI:102708. Efnb1.

    Phylogenomic databases

    eggNOGi NOG262190.
    HOGENOMi HOG000220931.
    HOVERGENi HBG051448.
    InParanoidi P52795.
    KOi K05463.
    OMAi PDSFFNS.
    OrthoDBi EOG7288S5.
    PhylomeDBi P52795.

    Miscellaneous databases

    NextBioi 284342.
    PROi P52795.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52795.
    Bgeei P52795.
    CleanExi MM_EFNB1.
    Genevestigatori P52795.

    Family and domain databases

    Gene3Di 2.60.40.420. 1 hit.
    InterProi IPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view ]
    PANTHERi PTHR11304. PTHR11304. 1 hit.
    Pfami PF00812. Ephrin. 1 hit.
    [Graphical view ]
    PRINTSi PR01347. EPHRIN.
    ProDomi PD002533. Ephrin. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF49503. SSF49503. 1 hit.
    PROSITEi PS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic organization and chromosomal localization of mouse Eplg2, a gene encoding a binding protein for the receptor tyrosine kinase elk."
      Fletcher F.A., Renshaw B., Hollingsworth T., Baum P., Lyman S.D., Jenkins N.A., Gilbert D.J., Copeland N.G., Davison B.L.
      Genomics 24:127-132(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    2. "Efficient cloning of cDNAs of retinoic acid-responsive genes in P19 embryonal carcinoma cells and characterization of a novel mouse gene, Stra1 (mouse LERK-2/Eplg2)."
      Bouillet P., Oulad-Abdelghani M., Vicaire S., Garnier J.-M., Schuhbaur B., Dolle P., Chambon P.
      Dev. Biol. 170:420-433(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "cDNA cloning and characterization of a ligand for the Cek5 receptor protein-tyrosine kinase."
      Shao H., Lou L., Pandey A., Pasquale E.B., Dixit V.M.
      J. Biol. Chem. 269:26606-26609(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. "Complementary expression of transmembrane ephrins and their receptors in the mouse spinal cord: a possible role in constraining the orientation of longitudinally projecting axons."
      Imondi R., Wideman C., Kaprielian Z.
      Development 127:1397-1410(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiEFNB1_MOUSE
    AccessioniPrimary (citable) accession number: P52795
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3