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P52793

- EFNA1_MOUSE

UniProt

P52793 - EFNA1_MOUSE

Protein

Ephrin-A1

Gene

Efna1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.3 Publications

    GO - Molecular functioni

    1. ephrin receptor binding Source: RefGenome
    2. protein binding Source: IntAct

    GO - Biological processi

    1. activation of MAPK activity Source: MGI
    2. angiogenesis Source: UniProtKB-KW
    3. aortic valve morphogenesis Source: BHF-UCL
    4. axon guidance Source: RefGenome
    5. cell migration Source: Ensembl
    6. endocardial cushion to mesenchymal transition involved in heart valve formation Source: BHF-UCL
    7. ephrin receptor signaling pathway Source: UniProtKB
    8. mitral valve morphogenesis Source: BHF-UCL
    9. negative regulation of dendritic spine morphogenesis Source: UniProtKB
    10. negative regulation of epithelial to mesenchymal transition Source: BHF-UCL
    11. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    12. neuron differentiation Source: MGI
    13. notochord formation Source: MGI
    14. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    15. regulation of angiogenesis Source: UniProtKB
    16. regulation of axonogenesis Source: MGI
    17. regulation of blood vessel endothelial cell migration Source: UniProtKB
    18. regulation of cell adhesion mediated by integrin Source: Ensembl
    19. regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
    20. substrate adhesion-dependent cell spreading Source: Ensembl

    Keywords - Biological processi

    Angiogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ephrin-A1
    Alternative name(s):
    EPH-related receptor tyrosine kinase ligand 1
    Short name:
    LERK-1
    Immediate early response protein B61
    Cleaved into the following chain:
    Gene namesi
    Name:Efna1
    Synonyms:Epgl1, Epl1, Lerk1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:103236. Efna1.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: UniProtKB
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 182165Ephrin-A1PRO_0000008355Add
    BLAST
    Chaini18 – ?Ephrin-A1, secreted formPRO_0000389631
    Propeptidei183 – 20523Removed in mature formSequence AnalysisPRO_0000008356Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi51 ↔ 92PROSITE-ProRule annotation
    Disulfide bondi80 ↔ 140PROSITE-ProRule annotation
    Lipidationi182 – 1821GPI-anchor amidated serineSequence Analysis

    Post-translational modificationi

    Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiP52793.
    PRIDEiP52793.

    PTM databases

    PhosphoSiteiP52793.

    Expressioni

    Gene expression databases

    ArrayExpressiP52793.
    BgeeiP52793.
    CleanExiMM_EFNA1.
    GenevestigatoriP52793.

    Interactioni

    Subunit structurei

    Monomer. Homodimer. Forms heterodimers with EPHA2. Binds to the receptor tyrosine kinases EPHA2, EPHA3, EPHA4, EPHA5, EPHA6 and EPHA7. Also binds with low affinity to EPHA1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EPHA7Q153752EBI-5241529,EBI-1383428From a different organism.

    Protein-protein interaction databases

    BioGridi199389. 3 interactions.
    IntActiP52793. 1 interaction.
    STRINGi10090.ENSMUSP00000029566.

    Structurei

    3D structure databases

    ProteinModelPortaliP52793.
    SMRiP52793. Positions 18-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 161144Ephrin RBDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ephrin family.PROSITE-ProRule annotation
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG296825.
    GeneTreeiENSGT00390000015107.
    HOGENOMiHOG000234373.
    HOVERGENiHBG051447.
    InParanoidiP52793.
    KOiK05462.
    OMAiPIHHQED.
    OrthoDBiEOG70W3FD.
    PhylomeDBiP52793.

    Family and domain databases

    Gene3Di2.60.40.420. 1 hit.
    InterProiIPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view]
    PANTHERiPTHR11304. PTHR11304. 1 hit.
    PfamiPF00812. Ephrin. 1 hit.
    [Graphical view]
    PRINTSiPR01347. EPHRIN.
    ProDomiPD002533. Ephrin. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF49503. SSF49503. 1 hit.
    PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52793-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFLWAPLLG LCCSLAAADR HIVFWNSSNP KFREEDYTVH VQLNDYLDII    50
    CPHYEDDSVA DAAMERYTLY MVEHQEYVAC QPQSKDQVRW NCNRPSAKHG 100
    PEKLSEKFQR FTPFILGKEF KEGHSYYYIS KPIYHQESQC LKLKVTVNGK 150
    ITHNPQAHVN PQEKRLQADD PEVQVLHSIG YSAAPRLFPL VWAVLLLPLL 200
    LLQSQ 205
    Length:205
    Mass (Da):23,802
    Last modified:October 1, 1996 - v1
    Checksum:i5A8F3A6E2091E868
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 741H → Y in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti79 – 791A → T in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti81 – 811Q → E in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti91 – 911N → K in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti94 – 941R → Q in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti112 – 1121T → S in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti115 – 1151I → T in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti138 – 1381S → T in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti154 – 1541N → S in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti156 – 1561Q → H in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti159 – 1591V → A in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti181 – 1811Y → H in BAA07344. (PubMed:7675446)Curated
    Sequence conflicti204 – 2041S → T in BAA07344. (PubMed:7675446)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38146 mRNA. Translation: BAA07344.1.
    U26188 mRNA. Translation: AAA67563.1.
    U90662 mRNA. Translation: AAB50237.1.
    BC002046 mRNA. Translation: AAH02046.1.
    CCDSiCCDS17501.1.
    RefSeqiNP_034237.3. NM_010107.4.
    UniGeneiMm.15675.

    Genome annotation databases

    EnsembliENSMUST00000029566; ENSMUSP00000029566; ENSMUSG00000027954.
    GeneIDi13636.
    KEGGimmu:13636.
    UCSCiuc008pyo.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38146 mRNA. Translation: BAA07344.1 .
    U26188 mRNA. Translation: AAA67563.1 .
    U90662 mRNA. Translation: AAB50237.1 .
    BC002046 mRNA. Translation: AAH02046.1 .
    CCDSi CCDS17501.1.
    RefSeqi NP_034237.3. NM_010107.4.
    UniGenei Mm.15675.

    3D structure databases

    ProteinModelPortali P52793.
    SMRi P52793. Positions 18-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199389. 3 interactions.
    IntActi P52793. 1 interaction.
    STRINGi 10090.ENSMUSP00000029566.

    PTM databases

    PhosphoSitei P52793.

    Proteomic databases

    PaxDbi P52793.
    PRIDEi P52793.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029566 ; ENSMUSP00000029566 ; ENSMUSG00000027954 .
    GeneIDi 13636.
    KEGGi mmu:13636.
    UCSCi uc008pyo.2. mouse.

    Organism-specific databases

    CTDi 1942.
    MGIi MGI:103236. Efna1.

    Phylogenomic databases

    eggNOGi NOG296825.
    GeneTreei ENSGT00390000015107.
    HOGENOMi HOG000234373.
    HOVERGENi HBG051447.
    InParanoidi P52793.
    KOi K05462.
    OMAi PIHHQED.
    OrthoDBi EOG70W3FD.
    PhylomeDBi P52793.

    Miscellaneous databases

    NextBioi 284320.
    PROi P52793.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52793.
    Bgeei P52793.
    CleanExi MM_EFNA1.
    Genevestigatori P52793.

    Family and domain databases

    Gene3Di 2.60.40.420. 1 hit.
    InterProi IPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view ]
    PANTHERi PTHR11304. PTHR11304. 1 hit.
    Pfami PF00812. Ephrin. 1 hit.
    [Graphical view ]
    PRINTSi PR01347. EPHRIN.
    ProDomi PD002533. Ephrin. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF49503. SSF49503. 1 hit.
    PROSITEi PS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of rat and mouse B61 gene: implications on organogenesis."
      Takahashi H., Ikeda T.
      Oncogene 11:879-883(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
    2. Morris J.C., Ciarletta A., Morris G.E., Giannotti J., Caruso A., Hammett D.J., Finnerty H., Turner K., Wood C.R.
      Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    3. "Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis."
      Flenniken A.M., Gale N.W., Yancopoulos G.D., Wilkinson D.G.
      Dev. Biol. 179:382-401(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    5. "Essential role of Vav family guanine nucleotide exchange factors in EphA receptor-mediated angiogenesis."
      Hunter S.G., Zhuang G., Brantley-Sieders D.M., Swat W., Cowan C.W., Chen J.
      Mol. Cell. Biol. 26:4830-4842(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Cdk5 regulates EphA4-mediated dendritic spine retraction through an ephexin1-dependent mechanism."
      Fu W.Y., Chen Y., Sahin M., Zhao X.S., Shi L., Bikoff J.B., Lai K.O., Yung W.H., Fu A.K., Greenberg M.E., Ip N.Y.
      Nat. Neurosci. 10:67-76(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DENDRITIC SPINE MORPHOGENESIS.
    7. "Identification and functional analysis of phosphorylated tyrosine residues within EphA2 receptor tyrosine kinase."
      Fang W.B., Brantley-Sieders D.M., Hwang Y., Ham A.-J.L., Chen J.
      J. Biol. Chem. 283:16017-16026(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiEFNA1_MOUSE
    AccessioniPrimary (citable) accession number: P52793
    Secondary accession number(s): P97331
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3